Shared Space, Shared Instruments, Shared Knowledge, Shared Science

a small painting of two proteins interacting with terminal beta sheets on a blue background.

The Protein Research Centre is an expertise platform for protein research at Utrecht University, providing a shared lab space for protein production, purification and characterization. Access is available for state-of-the-art equipment for all life science researchers.

Instruments

Our instruments are divided into techniques used for Biophysical analysis of proteins and protein-interactions, and techniques to purify the proteins. 

For more information on the Biophysical analysis techniques you can look under the "Techniques" tab. For selected publications where our techniques have been used, you can look at the bottom of this page below contact information. 

Biophysical Analysis

  • Fida 1

    Fida 1 (Flow Induced Dispersion Analysis) by FidaBio.

    See Techniques tap for more information.

  • Fida Neo

    Fida Neo (Flow Induced Dispersion Analysis) by FidaBio.

    See Techniques tap for more information.

  • Clariostar Plus

    Clariostar Plus (Fluorescence, Luminescence & Absorbance Plate Reader) by Isogen Life Sciences.

    See Techniques tap for more information.

  • Monolith

    Monolith (Microscale Thermophoresis) by NanoTemper.

    See Techniques tap for more information.

  • Prometheus Panta

    Prometheus Panta (Dynamic Light Scattering & Nano Differential Scanning Fluorimetry) by NanoTemper.

    See Techniques tap for more information.

  • Nanodrop one-C

    For accurately determining the protein concentration, DNA concentration or degree of labelling we have a nanodrop one-C available at our lab. 

Protein Purification

  • Akta Pure

    For protein purification we have 4 Akta pure M from Cytiva available as well as normal and high speed centrifuges, and SDS-page material. 

  • Akta Pure Micro

    For advanced protein purification we have 4 Akta Micro from Cytiva available as well as normal, high speed and ultra centrifuges, and SDS-page material. 

Contact

The Protein Research Centre is located at the Hugo R. Kruyt Building, on the east wing at the 8th floor. 

Address:
Padualaan 8,
3584 CH Utrecht

For questions, training and booking, please contact Renée Koopman:
Email: e.r.koopman@uu.nl
Phone nr.: 030 253 6454

Selected Publications

Fibril fragmentation generates diversity in seed population. Júlia Aragonès Pedrola, Marija Todorovska, Shalini Singh, Assaf Friedler, Stefan G. D. Rüdiger, bioRxiv 2024.11.12.623277; doi: https://doi.org/10.1101/2024.11.12.623277

FibrilPaint to determine the length of Tau amyloids in fluids. Júlia Aragonès Pedrola, Françoise A. Dekker, Tommaso Garfagnini, Guy Mayer, Margreet B. Koopman, Menno Bergmeijer, Friedrich Förster, Jeroen J. M. Hoozemans, Henrik Jensen, Assaf Friedler, Stefan G. D. Rüdiger, bioRxiv 2023.10.13.562220; doi: https://doi.org/10.1101/2023.10.13.562220

GAPR-1 Interferes with Condensate Formation of Beclin 1 in Saccharomyces cerevisiae. Sirati N, Shen Z, Olrichs NK, Popova B, Verhoek IC, Lagerwaard IM, Braus GH, Kaloyanova DV, Helms J, B.J Mol Biol. 2023 Feb 28;435(4):167935. 167935. Epub 2022 Dec 28. PMID: 36586462. doi: 10.1016/j.jmb.2022.