dr. R. (Rashmi) Sasidharan
R.Sasidharan@uu.nl
Gegenereerd op 2018-09-20 00:39:01


Profile

My early training as a biologist strongly biased me to think of organism responses at the cellular and molecular level. It was only during my PhD (University of Calgary, Canada) that I started to appreciate fully how carefully orchestrated cellular signaling events between tissues and organs modify and control whole plant behavior. What started as an investigation of the biochemistry of cell wall proteins, transformed serendipitously into a question that addressed whole plant ecophysiology and the fascinating shade-induced phenotypic plasticity (shade avoidance) displayed by ecotypes of the plant Stellaria longipes. It amazed me then as a novice plant biologist how identical environment signals could render completely opposite behavior in plants possessing the same genetic blueprint, but from a different habitat. Shade avoidance is considered one of the most striking examples of plant phenotypic plasticity. However, identical avoidance responses are displayed by certain plant species when flooded. The mechanisms mediating this submergence escape response in another genus Rumex displaying tremendous plasticity was the focus of my post-doctoral research at Utrecht University. Studying molecular mechanisms underlying an ecologically relevant question is a theme that has been the basis of my research.

My current work focuses on flooding survival mechanisms in plants. Climate change has greatly exacerbated the incidence and severity of flooding events and associated crop losses making this a very relevant research area. Although plant responses to flooding are well characterized, the genes and processes mediating the tremendous variation in tolerance and the molecular mechanisms underlying the survival strategies of wild species from flood-prone environments is largely unexplored. Using a combination of biochemical, molecular and genomic approaches in both wild species and Arabidopsis, we have been successful in identifying novel molecular processes and potential tolerance associated genes mediating survival strategies.

RESEARCH FUNDING:

2017 NWO-VIDI 'Microscopic lifeguards: stomatal contribution to plant resuscitation after flooding.'

2016 STW-Open technology program. Collaboration with the International Rice Research Institute (IRRI).‘Towards sustainable weed management solutions for the aggressive weed C. rotundus  

2015 NWO-ALW Crop Sciences. Collaboration with WUR and JNU New Delhi ‘Understanding responses to simultaneously and sequentially occurring abiotic stresses typical of climate change in rice and Arabidopsis.’

2015. NWO-ALW Open competition. Co-applicant. ‘A novel role for ethylene in conferring anoxia tolerance: mechanism and significance’ 

2012 NWO-Veni. 'After the rains: unravelling the molecular mechanisms driving post-flooding recovery in plants' 

2012 NWO-ALW. 'Flooding stress tolerance: an ecomolecular approach using Arabidopsis and wild relatives'.

2010 NWO-Ecogenomics ‘Escape from stress: a comparative ecogenomics approach.’ 

 

 

Involved in the following study programme(s)
Scientific expertise
plant abiotic stress
plant environmental interactions
plant responses to flooding and hypoxia
natural variation

Learning from nature. Wild plant species occupying flood prone regions are a potential source of novel tolerance mechanisms that can be translated to flood sensitive crop species. Rumex palustris and Rumex acetosa both inhabit wet areas with characteristic flooding regimes and display distinct survival strategies. Using this system, we are trying to identify the regulatory networks and novel genes and processes that mediate these adaptive growth regulatory responses to flooding. (Left) Images of Rumex palustris and Rumex acetosa which display distinct escape and quiescence survival strategies in response to flooding stress. (Centre) Gene families identified in the two Rumex species after transcriptome sequencing. The Venn diagram shows the overlap in gene families between Rumex and selected angiosperms. (Right) Schematic depiction of the regulatory network triggered by submergence and leading to shoot elongation in R. palustris.

Exploiting natural variation to understand flooding tolerance. (Left) Natural variation in submergence tolerance in 86 natural accessions of Arabidopsis thaliana. Tolerance is expressed as median lethal time-50 (LT50) or the number of days after which 50% of the population is dead. (Right-top) Scatter plots showing the overlap between transcriptome response to darkness (black dots) and submergence (in dark; red dots) of three tolerant and three intolerant accessions. Floods often severely reduce the light levels available to plants further restricting photosynthesis and depleting available carbon reserves. (Right-bottom) Natural variation in post-submergence recovery in two Arabidopsis accessions. Coping with terrestrial conditions post-flooding can be a stress in itself for flooded plants. We are using natural variation to identify genes and processes mediating faster post-submergence recovery in some genotypes.

Gegenereerd op 2018-09-20 00:39:01
Curriculum vitae

B.Sc (2001) Biochemistry. Delhi University, New Delhi. India.

M.Sc (2003) Biotechnology. Indian Institute of Technology, Bombay. India.

PhD (2009) Plant Developmental Biology. University of Calgary. Canada.

Post-doctoral researcher (2010) Plant Ecophysiology, Utrecht University, The Netherlands.

Molecular techniques in plant science (2010) Cold Spring Harbor Laboratory, USA.

Assistant professor (2010-2017) Plant Ecophysiology, Utrecht University, The Netherlands.

 

Gegenereerd op 2018-09-20 00:39:01
All publications
  2018 - Scholarly publications
Yeung, Elaine, van Veen, Hans, Vashisht, Divya, Paiva, Ana Luiza Sobral, Hummel, Maureen, Rankenberg, Tom, Steffens, Bianka, Steffen-Heins, Anja, Sauter, Margret, de Vries, Michel, Schuurink, Robert C., Bazin, Jérémie, Bailey-Serres, Julia, Voesenek, Laurentius A.C.J. & Sasidharan, Rashmi (26.06.2018). A stress recovery signaling network for enhanced flooding tolerance in Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America, 115 (26), (pp. E6085-E6094).
  2017 - Scholarly publications
Ravanbakhsh, Mohammadhossein, Sasidharan, Rashmi, Voesenek, Laurentius, Kowalchuk, George & Jousset, Alexandre (2017). ACC deaminase-producing rhizosphere bacteria modulate plant responses to flooding. Journal of Ecology, 105 (4), (pp. 979-986) (8 p.).
Sasidharan, Rashmi, Hartman, Sjon, Liu, Zeguang, Martopawiro, Shanice, Sajeev, Nikita, van Veen, Hans, Yeung, Elaine & Voesenek, Laurentius A C J (02.11.2017). Signal dynamics and interactions during flooding stress. Plant Physiology
  2016 - Scholarly publications
Das, Debatosh, St. Onge, Kate R., Voesenek, Laurentius A C J, Pierik, Ronald & Sasidharan, Rashmi (01.10.2016). Ethylene- and shade-induced hypocotyl elongation share transcriptome patterns and functional regulators. Plant Physiology, 172 (2), (pp. 718-733) (16 p.).
Voesenek, Laurentius, Sasidharan, Rashmi, Visser, Eric J W & Bailey, Julia (2016). Flooding stress signaling through perturbations in oxygen, ethylene, nitric oxide and light. New Phytologist, 209 (1), (pp. 39-43) (5 p.).
Sasidharan, R. (2016) Invited speaker GRC Salt & Water stress. Les Diablerets (01.01.2016)
Sasidharan, R. (2016) Invited speaker IEB symposium Utrecht (01.01.2016) Lecture: Braving the deluge: how plants cope with flooding stress.
Sasidharan, R. (2016) Invited speaker Scuola Superiore Santa Anna. Pisa (01.01.2016) Lecture: Flooding stress survival in plants: the importance of foresight and recovery.
Sasidharan, R. (2016) Invited speaker ISPA conference Elsinor (06.09.2016 - 08.09.2016) Lecture: Flooding stress survival: the importance of foresight.
Sasidharan, R. (2016) Invited speaker Future Deltas meets Future Food workshop. (01.01.2016) Lecture: Waterproof: plant survival strategies in a wetter world.
van Veen, Hans, Vashisht, Divya, Akman, Melis, Girke, Thomas, Mustroph, Angelika, Reinen, Emilie, Hartman, Sjon, Kooiker, Maarten, van Tienderen, Peter, Schranz, M. Eric, Bailey-Serres, Julia, Voesenek, Laurentius & Sasidharan, Rashmi (2016). Transcriptomes of Eight Arabidopsis thaliana Accessions Reveal Core Conserved, Genotype- and Organ-Specific Responses to Flooding Stress. Plant Physiology, 172 (2), (pp. 668-689) (22 p.). © 2016 American Society of Plant Biologists. All Rights Reserved..
Vashisht, Divya, van Veen, Hans, Akman, Melis & Sasidharan, Rashmi (2016). Variation in Arabidopsis flooding responses identifies numerous putative "tolerance genes". Plant Signaling and Behavior, 11 (11).
  2015 - Scholarly publications
Sasidharan, R. () Invited speaker 15th New Phytologist Workshop: ‘Flooding stress: signaling through perturbations in oxygen, ethylene, nitric oxide and light.’ Ravenstein (20.06.2015 - 21.06.2015)
Sasidharan, R. (01.01.2015 01.06.2015) Organiser 15th New Phytologist Workshop: ‘Flooding stress: signaling through perturbations in oxygen, ethylene, nitric oxide and light.’ Ravenstein (20.06.2015 - 21.06.2015) Organising committee: Rens Voesenek (Utrecht University, Utrecht, the Netherlands) Rashmi Sasidharan (Utrecht University, Utrecht, the Netherlands) Julia Bailey-Serres (University of California Riverside, CA, USA) Eric Visser (Radboud University Nij
Sasidharan, R. (20.06.2015) Invited speaker 15th New Phytologist Workshop: ‘Flooding stress: signaling through perturbations in oxygen, ethylene, nitric oxide and light.’ Ravenstein (20.06.2015 - 21.06.2015)
Sasidharan, R. (20.09.2015) Invited speaker FEBS workshop Plant Organeller Signaling Primosten (16.09.2015) Braving the deluge: molecular signaling mechanisms mediating plant responses to flooding stress. Closing lecture
Sasidharan, R. () Keynote speaker FEBS workshop Plant Organeller Signaling Primosten (16.09.2015) Braving the deluge: molecular signalling mechanisms mediating plant responses to flooding stress.
Sasidharan, Rashmi & Voesenek, Laurentius A C J (01.09.2015). Ethylene-mediated acclimations to flooding stress. Plant Physiology, 169 (1), (pp. 3-12) (10 p.).
Voesenek, L.A.C.J., Pierik, R. & Sasidharan, R. (2015). Plant Life without Ethylene. Trends in Plant Science, 20 (12), (pp. 783-786) (4 p.).
Riber, Willi, Müller, Jana T., Visser, Eric J W, Sasidharan, Rashmi, Voesenek, Laurentius A C J & Mustroph, Angelika (01.04.2015). The greening after extended darkness1 is an N-End rule pathway mutant with high tolerance to submergence and starvation. Plant Physiology, 167 (4), (pp. 1616-1629) (14 p.).
  2014 - Scholarly publications
Sasidharan, R. (15.07.2014) Participant ASPB Plant Biology Portland, OR (12.07.2014 - 16.07.2014) Downproofing: Flooding tolerance and plant survival strategies
Van Veen, Hans, Vashisht, Divya, Voesenek, Laurentius A C J & Sasidharan, Rashmi (2014). Different survival strategies amongst plants to cope with underwater conditions. In Joost T. van Dongen & Francesco Licausi (Eds.), Low-Oxygen Stress in Plants - Oxygen Sensing and Adaptive Responses to Hypoxia (pp. 329-349) (21 p.). Springer.
Akman, M., Bhikharie, A., Mustroph, A. & Sasidharan, Rashmi (2014). Extreme flooding tolerance in Rorippa. Plant Signaling and Behavior, 9 (2) (5 p.).
van Veen, Hans, Akman, V., Jamar, D.C.L., Vreugdenhil, D., Kooiker, Maarten, van Tienderen, P.H., Voesenek, Laurentius, Schranz, E. & Sasidharan, Rashmi (2014). Group VII Ethylene response factor diversification and regulation in four species from flood-prone environments. Plant, Cell and Environment, 37 (10), (pp. 2421-2432) (12 p.).
Mustroph, Angelika, Hess, Natalia & Sasidharan, Rashmi (2014). Hypoxic energy metabolism and ppi as an alternative energy currency. Plant Cell Monographs, 21, (pp. 165-184) (20 p.).
Sasidharan, Rashmi, Keuskamp, Diederik, Kooke, R., Voesenek, Laurentius & Pierik, Ronald (2014). Interactions between auxin, microtubules and XTHs mediate green shade-induced petiole elogation in Arabidopsis. PLoS One, 9 (3) (13 p.).
Voesenek, Laurentius, van Veen, Hans & Sasidharan, Rashmi (2014). Learning from Nature: the use of non-model species to identify novel acclimations to flooding stress. AoB PLANTS, 6 (6 p.).
  2014 - Popularising publications
Sasidharan, R. () Invited speaker Invited talk Taipei (07.05.2014) Flooding tolerance: exploiting natural variation in Arabidopsis
  2013 - Scholarly publications
Voesenek, L.A.C.J. & Sasidharan, R. (2013). Ethylene - and oxygen signaling-drive survival during flooding. Plant Biology, 15, (pp. 426-435) (10 p.).
Sasidharan, R. & Voesenek, L.A.C.J. (2013). Lowland rice: high-end submergence tolerance. New Phytologist, 197, (pp. 2229-2031) (199 p.).
Sasidharan, R. (01.10.2013) Invited speaker Meeting of the International society for Plant Anaerobiosis Los Banos, The Philippines (01.01.2013) Natural variation and flooding stress tolerance: lessons from Arabidopsis
Sasidharan, R., Mustroph, A., Boonman, A., Akman, M., Ammerlaan, J.M.H., Breit, T., Schranz, M.E., Voesenek, L.A.C.J. & van Tienderen, P.H. (2013). Root transcript profiling of two Rorippa (Brassicaceae) species reveals gene clusters associated with extreme submergence tolerance. Plant Physiology, 163, (pp. 1277-1292) (16 p.).
van Veen, H., Mustroph, A., Barding, G.A., Vergeer- van Eijk, M., Welschen, R.A.M., Pederson, O., Visser, E.J.W., Larive, C.K., Pierik, R., Bailey-Serres, J., Voesenek, L.A.C.J. & Sasidharan, R. (2013). Two Rumex species from contrasting hydrological niches, regulate flooding tolerance through distinct mechanisms. Plant Cell, 25, (pp. 4691-4707) (17 p.).
  2013 - Popularising publications
Sasidharan, R. (01.08.2013) Invited speaker Summerschool Environmental Signaling (Utrecht) (01.01.2013) Drownproofing: flooding tolerance and plant survival strategies
Sasidharan, R. (01.08.2013) Invited speaker Center for Biosystemes Genomics Summit (Wageningen) (01.01.2013) Waterproof plants: Exploiting natural variation in Arabidopsis to stydy flooding tolerance
  2012 - Scholarly publications
Sasidharan, R. & Mustroph, A. (2012). Plant oxygen sensing is mediated by the N-end rule pathway: a milestone in plant anaerobiosis. Plant Cell, 23, (pp. 4173-4183) (11 p.).
  2012 - Popularising publications
Sasidharan, R. (08.03.2012) Invited speaker Rice Research Institute (Los Banos, Filipijnene) (08.01.2012) Natural variation in the response to flodding stress among Arabidopsis accessions
Sasidharan, R. (06.03.2012) Invited speaker Kong Kong University (06.01.2012) Natural variation in the response to flooding stress among Arabidopsis accessions
  2011 - Scholarly publications
Keuskamp, D.H., Sasidharan, R., Peeters, A.J.M., Voesenek, L.A.C.J., Pierik, R. & Vos, I (2011). Blue light-meditated shade avoidance requires combined auxin and brassinosteroid action in Arabidopsis seedlings. Plant Journal, 67, (pp. 208-217) (10 p.).
Sasidharan, R., Voesenek, L.A.C.J. & Pierik, R. (2011). Cell-wall modifying proteins mediate plant acclimatization to biotic and abiotic stresses. Critical Reviews in Plant Sciences, 30, (pp. 548-562) (15 p.).
Lee, S.C., Mustroph, A., Sasidharan, R., Vashisht, D., Pederson, O., Oosumi, T., Voesenek, L.A.C.J. & Bailey-Serres, J. (2011). Molecular characterization of the submergence response of the Arabisopsis thaliana ecotype Columbia. New Phytologist, 190, (pp. 457-471) (15 p.).
Vashisht, D, Hesselink, A, Pierik, R, Ammerlaan, J M H, Bailey-Serres, J, Visser, E J W, Pedersen, O, van Zanten, M, Vreugdenhil, D, Jamar, D C L, Voesenek, L A C J & Sasidharan, R (2011). Natural variation of submergence tolerance among Arabidopsis thaliana accessions. New Phytologist, 190 (2), (pp. 299-310) (12 p.). © 2010 The Authors. New Phytologist © 2010 New Phytologist Trust..
Sasidharan, R. & Mustroph, A. (2011). Plant Oxygen Sensing is Meditated by the N-End Rule Pathway: a Milestone in Plant Anaerobiosis. Plant Cell
Sasidharan, R. (05.04.2011) Invited speaker EPS Meeting Lunteren (02.04.2012) Shoot elongation during shade avoidance. the role of cortical microtubules
  2011 - Popularising publications
Sasidharan, R. (31.01.2011) Invited speaker CBSG annual summit (Wageningen) (31.01.2011) Flooding stress in plants: exploting natural variation in Arabidopsis thaliana
  2010 - Scholarly publications
Heydarian, Z., Sasidharan, R., Cox, M.C.H., Pierik, R., Voesenek, L.A.C.J. & Peeters, A.J.M. (2010). A kinetic analysis of hyponastic growth and petiole elongation upon ethylene exposure in Rumex palustris. Annals of Botany, 106, (pp. 429-435) (7 p.).
Sasidharan, R. & Pierik, R. (2010). Cell wall modification involving XTHs controls phytochrome-mediated petiole elongation in Arabidopsis thaliana. Plant Signaling and Behavior, 5, (pp. 1491-1492) (2 p.).
Sasidharan, R., Chinnappa, C.C., Staal, M., Elzenga, J.T.M., Yokoyama, R., Nishitani, K., Voesenek, L.A.C.J. & Pierik, R. (2010). Light quality-mediated petiole elongation in Arabidopsis during shade avoidance involves cell wall modification by XTHs. Plant Physiology, 154, (pp. 978-990) (13 p.).
Keuskamp, D.H., Sasidharan, R. & Pierik, R. (2010). Physiological regulation and functional significance of shade avoidance responses to neighbours. Plant Signaling and Behavior, 5 (6), (pp. 655-662) (8 p.).
Sasidharan, R. (09.02.2010) Invited speaker Netherlands Annual Ecology Meeting Lunteren (07.02.2012 - 08.02.2012) The regulation of cell wall extensibility during shade avoidance.
  2009 - Scholarly publications
Sasidharan, R., Chinnappa, C.C., Voesenek, L.A.C.J. & Pierik, R. (2009). A molecular basis for the physiological variation in shade avoidance responses : a tale of two ecotypes. Plant Signaling and Behavior, 4, (pp. 528-529) (2 p.).
Sasidharan, R. (01.09.2009) Invited speaker Experimental Plant Sciences Meeting Wageningen, The Netherlands (01.01.2009) Growth regulation during shade avoidance: the role of the cell wall.
Li, W., Song, Z., Sasidharan, R. & Chinnappa, C.C. (2009). Light and shade signals regulate four phytochrome A genes in Stellaria longipe. International Journal of Plant Sciences, 170, (pp. 164-173) (10 p.).
Pierik, R., Keuskamp, D.H., Sasidharan, R., Djakovic-Petrovic, T., de Wit, M. & Voesenek, L.A.C.J. (2009). Light quality controls shoot elongation through regulation of multiple hormones. Plant Signaling and Behavior, 4, (pp. 755-756) (2 p.).
Sasidharan, R. (01.02.2009) Invited speaker Netherlands Annual Ecology Meeting Lunteren, The Netherlands (11.02.2009) The regulation of cell wall extensibility during shade avoidance: a study using two contrasting ecotypes of Stellaria longpipes.
Sasidharan, R. (01.06.2009) Invited speaker Ethylene Conference Cornell Ithaca, NY, USA (01.01.2009) The role of ethylene during flooding responses in Rumex palustris.
  2009 - Popularising publications
Sasidharan, R. (01.02.2009) Invited speaker Invited lecture Netherlands ecology research network (NERN). (Lunteren, The Netherlands) (01.01.2009) Shade avoidance and the regulation of cell wall extensibility in two ecotypes of Stellaria longipes.
Sasidharan, R. (01.06.2009) Invited speaker Keynote lecture 8th International symposium on the plant hormone ethylene. Cornell University (Ithaca, NY, USA) (01.01.2009) Struggling for light: regulation of plant-plant interactions.
  2008 - Scholarly publications
Sasidharan, R., Chinnappa, C.C., Voesenek, L.A.C.J. & Pierik, R. (2008). The regulation of cell wall extensibility during shade avoidance: a study using two contrasting ecotypes of Stellaria longipes. Plant Physiology, 148, (pp. 1557-1569) (13 p.).
  2008 - Popularising publications
Sasidharan, R. (07.04.2008) Invited speaker ALW Discussieplatform Experimentele Plantenwetenschappen (EPW) (Lunteren) (07.01.2008) The regulation of cell wall extensibility during shade avoidance.
  2007 - Scholarly publications
Pierik, R., Sasidharan, R. & Voesenek, L.A.C.J. (2007). Growth control by ethylene: Adjusting phenotypes to the environment. Journal of Plant Growth Regulation, 26, (pp. 188-200) (13 p.).
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Gegenereerd op 2018-09-20 00:39:02
Additional functions and activities

 

Editorial board member Scientific Reports

Department Advice Committee member 2015-current

Young Investigator Forum within Science for Life. https://tinyurl.com/ycddd29h

 

 

Gegenereerd op 2018-09-20 00:39:02
Full name
dr. R. Sasidharan Contact details
Hugo R. Kruytgebouw

Padualaan 8
Room Z306
3584 CH  UTRECHT
The Netherlands


Phone number (direct) +31 30 253 6871
Phone number (department) +31 30 253 6871
Gegenereerd op 2018-09-20 00:39:02
Last updated 16.05.2018