prof. dr. J.B. (Bernd) Helms
J.B.Helms@uu.nl
Gegenereerd op 2017-09-23 23:47:50


Chair
Veterinaire Biochemie
Endowed by Utrecht University
Date of appointment 01.04.2002
Inaugural lecture date 25.09.2003
Profile

Bernd Helms studies the intracellular membrane dynamics of mammalian cells. His major expertise is in the area of protein and lipid biochemistry. Currently, his research focusses on lipid & membrane dynamics and the application of lipidomic techniques to study the function of lipids and lipid droplets in the pathogenesis of veterinary and human diseases.  

Our view of biological membrane dynamics has evolved dramatically over the last few decades. Initially a static model was proposed by Singer and Nicholson that describes proteins stochastically attached to and embedded in a lipid bilayer. During the last two decades, this view has changed and we now envision membranes as highly dynamic structures. In the cell, membranes are continuously transported between organelles. This process includes protein and lipid sorting during transport to maintain the unique membrane composition of each organelle. Within membranes, selected lipids and proteins can dynamically segregate in microdomains to regulate cellular processes.

His research goal is to study the dynamic properties of biological membranes that play a crucial role in normal cell physiology and in many diseases that result from dysfunction of membrane dynamics. Key research include (i) membrane dynamics in host – pathogen interactions, focusing on Salmonella typhimurium and on GAPR-1; (ii) membrane dynamics of lipid droplets in normal and diseased liver  focusing on the role of hepatic stellate cells in liver regeneration; (iii) membrane dynamics during fertilization, focusing on sperm and oocyte membrane dynamics during the fusion process, and (iv) the development and application of lipidomic techniques to fundamental, applied, and clinical research. Bernd Helms collaborates with national and international biochemical, biomedical and cell biological research groups in these areas. The projects provide a molecular basis for understanding diseases, which is crucial to develop new strategies to develop novel drugs for the benefit of in human and animal health.

Key publications

  • Helms, J.B. and Rothman, J.E. (1992) Inhibition by Brefeldin A of a Golgi membrane enzyme that catalyses exchange of guanine nucleotide bound to ARF. Nature 360, 352-354.
  • Helms, J.B., Palmer, D.J., and Rothman, J.E. (1993) Two distinct populations of ARF bound to Golgi membranes. J. Cell Biol. 121, 751-760.
  • Helms, J.B., Helms-Brons, D., Brügger, B., Gkantiragas, I., Eberle, H., Nickel, W., Nürnberg, B., Gerdes, H-H., and Wieland, F.T. (1998) A putative heterotrimeric G protein inhibits the fusion of COPI coated vesicles. Segregation of heterotrimeric G proteins from COPI-coated vesicles. J. Biol. Chem 273: 15203 15208.
  • Gkantiragas, I., Brügger,B., Stüven, E., Kaloyanova, D., Li, X-Y., Löhr, K., Lottspeich, F., Wieland, F.T. and Helms, J.B. Sphingomyelin-enriched microdomains at the Golgi complex. (2001) Mol. Biol Cell 12, 1819-1833.
  • Eberle, H.B., Serrano, R.L., Füllekrug, J., Schlosser, M., Lehmann, W.D., Lottspeich, F., Kaloyanova, D., Wieland, F.T., and Helms, J.B. Identification and characterization of a novel human plant pathogenesis-related protein that localizes to lipid-enriched microdomains in the Golgi complex. (2002) J. Cell Sci. 115, 827-838.
  • Stüven, E., Porat, A., Shimron, F., Fass, E., Kaloyanova, D., Brügger, B., Wieland, F.T., Elazar, Z. and Helms, J.B. Intra-Golgi protein transport depends on a cholesterol balance in the lipid membrane. (2003) J. Biol. Chem. 278, 53112-53122.
  • Helms, J.B. and Zurzolo, C. Lipids as targeting signals: lipid rafts and intracellular trafficking (2004) Traffic 5, 247-254.
  • Serrano, R.L., Kuhn A., Hendricks A., Helms J.B., Sinning I., and Groves M.R. Structural analysis of the human Golgi-associated plant pathogenesis related protein GAPR-1 implicates dimerization as a regulatory mechanism (2004) J. Mol. Biol. 339, 173-183.
  • Li, X., Kaloyanova, D., van Eijk, M., Eerland, R., van der Goot, G., Oorschot, V., Klumperman, J., Lottspeich, F., Starkuviene, V., Wieland, F.T., and Helms, J.B. (2007) Involvement of a GPI-anchored Protein in Maintenance of the Golgi Structure. Mol. Biol. Cell 18, 1261-1271.
  • Testerink, N., Van der Sanden, M.H.M., Houweling, M., Helms, J.B., and Vaandrager, A.B., Depletion of phosphatidylcholine affects endoplasmic reticulum morphology and protein traffic at the Golgi complex.  (2009) J. Lipid Res. 50, 2182-2206
  • Van der Meer-Janssen, Y.P.M., van Galen, J., Batenburg, J.J., and Helms, J.B. Lipids in host-pathogen interactions: pathogens exploit the complexity of the host cell lipidome (2010) Prog. Lipid Res. 49, 1-26
  • Van Galen, J., Van Balkom, B.W.M., Serrano, R.L., Kaloyanova, D., Eerland, R., Stüven, E., and Helms, J.B. Binding of the GAPR-1 to negatively charged phospholipid membranes: Unusual binding characteristics to phosphatidylinositol (2010) Mol. Mem. Biol. 27, 81-91
  • Vogels, M.W., van Balkom, B.W.M., Heck, A.J., de Haan, C.A.M.,  Rottier, P.J.M., , Batenburg, J.J., Kaloyanova, D.V., Helms, J.B. Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle (2011) Proteomics 11, 4477-4491
  • Testerink, M., Ajat, M.A., Houweling, M., Brouwers, J.F., Pully, V.V., van Manen, H-J., Otto, C., Helms, J.B., and Vaandrager, A.B. Replacement of retinyl esters by polyunsaturated triacylglycerol species in lipid droplets of hepatic stellate cells during activation (2012) PLoS ONE 7, e34945
  • Van Galen, J., Olrichs, N., Schouten, A., Serrano, R.L., Note-'t Hoen, E., Eerland, R., Kaloyanova, D., Gros, P. and J. Bernd Helms. Interaction of GAPR-1 with lipid bilayers is regulated by alternative homodimerization (2012) BBA-Biomembranes 1818, 2175–2183
  • Shoji-Kawata, S., Sumpter, R., Leveno, M., Campell, G.R., Zou, Z., Kinch, L., Wilkins, A.D., Sun, Q., Pallauf, K., MacDuff, D., Huerta, C., Virgin, H.W., Helms, J.B., Eerland, R., Tooze, S.A., Xavier, R., Lenschow, D.J., Yamamoto, A., King, D., Lichtarge, O., Grishin, N.V., Spector, S.A., Kaloyanova, D.V., and Beth Levine (2013) Identification of a candidate therapeutic autophagy-inducing peptide. Nature 494, 201-206 
Scientific expertise
lipids
lipid droplets
lipidomics
hepatic stellate cells
GAPR-1 protein
CAP/SCP/PR-1 superfamily of proteins
Gegenereerd op 2017-09-23 23:47:51
Curriculum vitae

Utrecht University                                                                                         1980-1986           School of Chemistry

Utrecht University                                                                                         1986-1991           Center for Biomembranes and Lipid Enzymology                                                                      Graduate study 

Post-doctoral research fellow                                                                         1991-1994           Memorial Sloan-Kettering Cancer Center (New York, USA)

Post-doctoral research fellow                                                                         1994-1996           University of Heidelberg, Germany 

Junior group leader                                                                                      1997-2002           Biochemie-Zentrum Heidelberg (BZH)                                                                                    Ruprecht-Karls-University of Heidelberg, Germany 

Professor of Veterinary Biochemistry                                                                      2002-          Faculty of Veterinary Medicine                                                                                                  Utrecht University, The Netherlands

Chairman Department of Biochemistry and Cell Biology                                           2004-          Faculty of Veterinary Medicine                                                                                                  Utrecht University, The Netherlands 

Director Institute of Biomembranes                                                                       2010-          Utrecht University 

Gegenereerd op 2017-09-23 23:47:51
All publications
  Articles - 2017
Ajat, Mokrish, Molenaar, Martijn, Brouwers, Jos F H M, Vaandrager, Arie B., Houweling, Martin & Helms, J. Bernd (01.02.2017). Hepatic stellate cells retain the capacity to synthesize retinyl esters and to store neutral lipids in small lipid droplets in the absence of LRAT. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1862 (2), (pp. 176-187) (12 p.).
Kruitwagen, Hedwig S, Oosterhoff, Loes A, Vernooij, Ingrid G W H, Schrall, Ingrid M, van Wolferen, Monique E, Bannink, Farah, Roesch, Camille, van Uden, Lisa, Molenaar, Martijn R, Helms, J Bernd, Grinwis, Guy C M, Verstegen, Monique M A, van der Laan, Luc J W, Huch, Meritxell, Geijsen, Niels, Vries, Robert G, Clevers, Hans, Rothuizen, Jan, Schotanus, Baukje A, Penning, Louis C & Spee, Bart (2017). Long-Term Adult Feline Liver Organoid Cultures for Disease Modeling of Hepatic Steatosis. Stem Cell Reports Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved..
Tuohetahuntila, Maidina, Molenaar, Martijn R, Spee, Bart, Brouwers, Jos F, Wubbolts, Richard, Houweling, Martin, Yan, Cong, Du, Hong, VanderVen, Brian C, Vaandrager, Arie B & Helms, J Bernd (14.06.2017). Lysosome-mediated degradation of a distinct pool of lipid droplets during hepatic stellate cell activation. Journal of Biological Chemistry Copyright © 2017, The American Society for Biochemistry and Molecular Biology..
Kong, Pengfei, Ufermann, Christoph-Martin, Zimmermann, Diana Lm, Yin, Qing, Suo, Xun, Helms, J Bernd, Brouwers, Jos F & Gupta, Nishith (2017). Two phylogenetically and compartmentally distinct CDP-diacylglycerol synthases cooperate for lipid biogenesis in Toxoplasma gondii. Journal of Biological Chemistry Copyright © 2017, The American Society for Biochemistry and Molecular Biology..
  Articles - 2016
Maidina, Tuohetahuntila, Molenaar, Martijn R, Spee, Bart, Brouwers, Jos F, Houweling, Martin, Vaandrager, Arie B & Helms, J Bernd (14.05.2016). ATGL and DGAT1 are involved in the turnover of newly synthesized triacylglycerols in hepatic stellate cells. Journal of Lipid Research, 57, (pp. 1162-1174). Copyright © 2016, The American Society for Biochemistry and Molecular Biology..
Olrichs, N.K. & Helms, J.B. (21.04.2016). Novel insights into the function of the conserved domain of the CAP superfamily of proteins. AIMS Biophysics, 3 (2), (pp. 232-246).
  Articles - 2015
Brachet, Anna, Norwood, Stephanie, Brouwers, Jos F, Palomer, Ernest, Helms, J Bernd, Dotti, Carlos G & Esteban, José A (2015). LTP-triggered cholesterol redistribution activates Cdc42 and drives AMPA receptor synaptic delivery. Journal of Cell Biology, 208 (6), (pp. 791-806) (16 p.). © 2015 Brachet et al..
Arroyo-Olarte, Ruben D, Brouwers, Jos F, Kuchipudi, Arunakar, Helms, J Bernd, Biswas, Aindrila, Dunay, Ildiko R, Lucius, Richard & Gupta, Nishith (2015). Phosphatidylthreonine and Lipid-Mediated Control of Parasite Virulence. PLoS Biology, 13 (11) (24 p.).
Kaloyanova, Dora, Vogels, Mijke, van Balkom, Bas W M & Helms, J Bernd (2015). Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle. Methods in Molecular Biology, 1225, (pp. 29-45) (17 p.).
Tuohetahuntila, Maidina, Spee, Bart, Kruitwagen, Hedwig S, Wubbolts, Richard, Brouwers, Jos F, van de Lest, Chris H, Molenaar, Martijn R, Houweling, Martin, Helms, J Bernd & Vaandrager, Arie B (2015). Role of long-chain acyl-CoA synthetase 4 in formation of polyunsaturated lipid species in hepatic stellate cells. Biochimica et Biophysica Acta, 1851 (2), (pp. 220-30) (11 p.). Copyright © 2014 Elsevier B.V. All rights reserved..
Helms, Bernd, Kaloyanova, Dora V, Strating, Jeroen R P, van Hellemond, Jaap J, van der Schaar, Hilde M, Tielens, Aloysius G M, van Kuppeveld, Frank J M & Brouwers, Jos F (2015). Targeting of the hydrophobic metabolome by pathogens. Traffic, 16 (5), (pp. 439-460) (22 p.). This article is protected by copyright. All rights reserved..
Lolicato, Francesca, Brouwers, Jos F., van de Lest, Chris H.A., Wubbolts, Richard, Aardema, Hilde, Priore, Paola, Roelen, Bernard A.J., Helms, J. Bernd & Gadella, Bart M (2015). The Cumulus Cell Layer Protects Bovine Maturing Oocyte Against Fatty Acid-Induced Lipotoxicity. Biology of Reproduction, 92 (1) (16 p.).
van Gestel, Renske A, Brouwers, Jos F, Ultee, Anton, Helms, J Bernd & Gadella, Bart M (16.09.2015). Ultrastructure and lipid composition of detergent-resistant membranes derived from mammalian sperm and two types of epithelial cells. Cell and Tissue Research, 363 (1), (pp. 129-145) (17 p.).
  Articles - 2014
Olrichs, Nick K, Mahalka, Ajay K, Kaloyanova, Dora, Kinnunen, Paavo K & Helms, Bernd (2014). Golgi-Associated plant Pathogenesis Related protein 1 (GAPR-1) forms amyloid-like fibrils by interaction with acidic phospholipids and inhibits Aβ aggregation. Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis, 21 (2), (pp. 88-96) (9 p.).
Houthuijzen, Julia M, Daenen, Laura G M, Roodhart, Jeanine M L, Oosterom, Ilse, van Jaarsveld, Marijn T M, Govaert, Klaas M, Smith, Michelle E, Sadatmand, Sahar J, Rosing, Hilde, Kruse, Fabian, Helms, Bernd J, van Rooijen, Nico, Beijnen, Jos H, Haribabu, Bodduluri, van de Lest, Chris H A & Voest, Emile E (2014). Lysophospholipids secreted by splenic macrophages induce chemotherapy resistance via interference with the DNA damage response. Nature Communications, 5 (10 p.).
Boerke, Arjan, van der Lit, Joost, Lolicato, Francesca, Stout, Tom A E, Helms, J Bernd & Gadella, Bart M (2014). Removal of GPI-anchored membrane proteins causes clustering of lipid microdomains in the apical head area of porcine sperm. Theriogenology, 81 (4), (pp. 613-624) (12 p.). Copyright © 2014 Elsevier Inc. All rights reserved..
  Articles - 2013
Aardema, Hilde, Lolicato, Francesca, van de Lest, Chris H A, Brouwers, Jos F, Vaandrager, Arie B, van Tol, Helena T A, Roelen, Bernard A J, Vos, Peter L A M, Helms, J Bernd & Gadella, Bart M (2013). Bovine cumulus cells protect maturing oocytes from increased fatty acid levels by massive intracellular lipid storage. Biology of Reproduction, 88 (6), (pp. 1-15) (15 p.).
Brouwers, J.F.H.M., Aalberts, M., Jansen, J.W.A., van Niel, G., Wauben, M.H.M., Stout, T.A.E., Helms, J.B. & Stoorvogel, W. (2013). Distinct lipid compositions of two types of human prostasomes. Proteomics, 13, (pp. 1660-1666) (7 p.).
Shoji-Kawata, S., Sumpter Jr, R., Leveno, M., Campbell, G.R., Zou, Z., Kinch, L., Wilkins, A.D., Sun, Q., Pallauf, K., MacDuff, D., Huerta, C., Virgin, H.W., Helms, J.B., Eerland, R., Tooze, S.A., Xavier, R., Lenschow, D.J., Yamamoto, A., King, D., Lichtarge, O., Grishin, N.V., Spector, S.A., Kaloyanova, D.V. & Levine, B. (2013). Identification of a candidate therapeutic autophagy-inducing peptide. Nature, 494, (pp. 201-206) (6 p.).
Boerke, Arjan, Brouwers, Jos F., Olkkonen, Vesa M., van de Lest, Chris H A, Sostaric, Edita, Schoevers, Eric J., Helms, J. Bernd & Gadella, Barend M. (01.01.2013). Involvement of Bicarbonate-Induced Radical Signaling in Oxysterol Formation and Sterol Depletion of Capacitating Mammalian Sperm During In Vitro Fertilization. Biology of Reproduction, 88 (1), (pp. 1-18) (18 p.).
Helms, J.B. (31.01.2013). Klein eiwit valt virussen en Alzheimer aan. De Volkskrant [online], (pp. 20) (1 p.).
Karasawa, A., Swier, L.J.Y.M., Stuart, M.C.A., Brouwers, J.F.H.M., Helms, J.B. & Poolman, B. (2013). Physicochemical Factors Controlling the Activity and Energy Coupling of an Ionic Strength-gated ATP-binding Cassette (ABC) Transporter. Journal of Biological Chemistry, Oct 11 (288 (41)), (pp. 29862-29871) (11 p.).
  Articles - 2012
Sodero, A.O., Vriens, J., Ghosh, D., Stegner, D., Brachet, A., Pallotto, M., Sassoe-Pognetto, M., Brouwers, J.F.H.M., Helms, J.B., Nieswandt, B., Voets, T. & Dotti, C.G. (2012). Cholesterol loss during glutamate-mediated excitotoxicity. EMBO Journal, 31, (pp. 1764-1773) (10 p.).
van Galen, Josse, Olrichs, Nick K, Schouten, Arie, Serrano, Ramon L, Nolte-'t Hoen, Esther N M, Eerland, Ruud, Kaloyanova, Dora, Gros, Piet & Helms, J Bernd (2012). Interaction of GAPR-1 with lipid bilayers is regulated by alternative homodimerization. Biochimica et Biophysica Acta, 1818 (9), (pp. 2175-2183) (9 p.). Copyright © 2012 Elsevier B.V. All rights reserved..
Cebollero, E., van der Vaart, A., Zhao, M., Rieter, E.M., Klionsky, D.J., Helms, J.B. & Reggiori, F.M. (2012). Phosphatidylinositol-3-Phosphate Clearance Plays a Key Role in Autophagosome Completion. Current Biology, 22 (17), (pp. 1545-1553) (9 p.).
Testerink, N., Md Ajat, M. M., Houweling, M., Brouwers, J.F.H.M., Pully, V.V., van Manen, H.W.J., Otto, C., Helms, J.B. & Vaandrager, A.B. (2012). Replacement of Retinyl Esters by Polyunsaturated Triacylglycerol Species in Lipid Droplets of HepaticStellate Cells during Activation. PLoS One, 7 (4), (pp. 10.1371/journal.pone.0034945) (11 p.).
Helms, J.B. (2012). Smeermiddel van de Cel. C2W Life Sciences 21, (pp. 24-25) (2 p.).
  Articles - 2011
Vogels, M.W., van Balkom, B.W.M., Kaloyanova, D.V., Batenburg, J.J., Heck, A.J.R., Helms, J.B., Rottier, P.J.M. & de Haan, C.A.M. (2011). Identification of host factors involved in coronavirus replication by quantitative proteomics analysis. Proteomics, 11 (1), (pp. 64-80) (17 p.).
Brouwers, J.F.H.M., Boerke, A., da Silva, P.F.N., Garcia-Gil, N., van Gestel, R.A., Helms, J.B., van de Lest, C.H.A. & Gadella, B.M. (2011). Mass Spectrometric detection of cholestrol oxidation in bovine sperm. Biology of Reproduction, 85, (pp. 128-136) (9 p.).
Aardema, H., Vos, P.L.A.M., Lolicato, F., Roelen, B.A.J., Knijn, H.M., Vaandrager, A.B., Helms, J.B. & Gadella, B.M. (2011). Oleic Acid Prevents Detrimental Effects of Saturated Fatty Acids on Bovine Oocyte. Biology of Reproduction, 85 (1), (pp. 62-69) (8 p.).
Vogels, M.W., van Balkom, B.W.M., Heck, A.J.R., de Haan, C.A.M., Rottier, P.J.M., Batenburg, J.J., Kaloyanova, D.V. & Helms, J.B. (2011). Quantitative proteomic identification of host factors involved in the Salmonella syphimuruim infectin cycle. Proteomics, 11, (pp. 4477-4491) (14 p.).
  Articles - 2010
Bot, M., Bot, I., Lopez-Vales, R., van de Lest, C.H.A., Saulnier-Blache, J.S., Helms, J.B., David, S., van Berkel, T.J.C. & Biessen, E.A.L. (2010). Atherosclerotic lesion progression changes lysophosphatidic acid homeostasis to favor ist accumulation. American Journal of Pathology, 176 (6), (pp. 3073-3084) (12 p.).
van Galen, J., van Balkom, B.W.M., Serrano, R.L., Kaloyanova, D.V., Eerland, R., Stuven, E. & Helms, J.B. (2010). Binding of GAPR-1 to negatively charged phospholipid membranes: unusual binding characteristics to phosphatidylinositol. Molecular Membrane Biology, 27 (2-3), (pp. 81-91) (10 p.).
van der Meer, Y.P.M., van Galen, J., Batenburg, J.J. & Helms, J.B. (2010). Lipids in host–pathogen interactions: Pathogens exploit the complexity of the host cell lipidome. Progress in Lipid Research, 49, (pp. 1-26) (27 p.).
  Articles - 2009
Testerink, N., van der Sanden, M.H.M., Houweling, M., Helms, J.B. & Vaandrager, A.B. (2009). Depletion of phosphatidylcholine affects ER morphology and protein traffic at the Golgi complex. Journal of Lipid Research, 50, (pp. 2182-2206) (24 p.).
Verheijen, M.H.G., Camargo, N., Verdier, V., Nadra, K., de Preux, A., Medard, J., Luoma, A., Crowther, M., Inouye, H., Shimano, H., Chen, S., Brouwers, J.F.H.M., Helms, J.B., Feltri, M.L., Wrabetz, L., Kirschner, D., Chrast, R. & Smit, A.B. (2009). SREBP cleavage activation protein (SCAP) is required for timely synthesis of a Schwann cell myelin membrane. Proceedings of the National Academy of Sciences of the United States of America, 106, (pp. 21383-21388) (6 p.).
van de Wetering, K., Feddema, W., Helms, J.B., Brouwers, J.F.H.M. & Borst, P. (2009). Targeted metabolomics iIdentifies glucuronides of dietary phytoestrogens as a major class of MRP3 substrates in vivo. Gastroenterology, 137, (pp. 1725-1735) (11 p.).
  Articles - 2007
Li, X., Kaloyanova, D.V., van Eijk, M., Eerland, R., van der Goot, G., Oorschot, V.M.J., Klumperman, J., Lottspeich, F., Starkuviene, V., Wieland, F.T. & Helms, J.B. (2007). Involvement of a Golgi-resident GPI-anchored protein in maintenance of the Golgi structure. Molecular Biology of the Cell, 18 (4), (pp. 1261-1271) (11 p.).
Huitema, L.F.A., van Weeren, R., van Balkom, B.W.M., Visser, T., van de Lest, C.H.A., Barneveld, A., Helms, J.B. & Vaandrager, A.B. (2007). Soluble factors released by ATDC5 cells affect the formation of calcium phosphate crystals. Biochimica et Biophysica Acta - Proteins and Proteomics, 1774, (pp. 1108-1117) (10 p.).
Bleijerveld, O.B., Brouwers, J.F.H.M., Vaandrager, A.B., Helms, J.B. & Houweling, M. (2007). The CDP-ethanolamine pathway and phosphatidylserine decarboxylation generate different phosphatidylethanolamine molecular species. Journal of Biological Chemistry, 282, (pp. 28362-28372) (11 p.).
  Articles - 2006
Helms, J.B. (2006). Host-Pathogen interactions: Lipids grease the way. European Journal of Lipid Science and Technology, 108, (pp. 895-897) (3 p.).
Huitema, L.F.A., van Weeren, P.R., Barneveld, A., van de Lest, C.H.A., Helms, J.B. & Vaandrager, A.B. (2006). Iron ions derived from the nitric oxide donor sodium nitroprusside inhibit mineralization. European Journal of Pharmacology, 542, (pp. 48-53) (6 p.).
Helms, J.B. & van Meer, G. (2006). Lipidome and disease. FEBS Letters, 580, (pp. 5429) (1 p.).
Huitema, L.F.A., Vaandrager, A.B., van Weeren, P.R., Barneveld, A., Helms, J.B. & van de Lest, C.H.A. (2006). The nitric oxide donor sodium nitroprusside inhibits mineralization in ATDC5 cells. Calcified Tissue International, 78 (3), (pp. 171-177) (7 p.).
  Articles - 2005
van Gestel, R.A., Brewis, I.A., Ashton, P.R., Helms, J.B., Brouwers, J.F.H.M. & Gadella, B.M. (2005). Capacitation-dependent concentration of lipid rafts in the apical ridge head area of porcine sperm cells. Molecular Human Reproduction, 11 (8), (pp. 583-590) (8 p.).
van Gestel, R.A., Helms, J.B., Brouwers, J.F.H.M. & Gadella, B.M. (2005). Effects of methyl-beta-cyclodextrin-mediated cholesterol depletion in porcine sperm compared to somatic cells. Molecular Reproduction and Development, 72, (pp. 386-395) (10 p.).
Kokubo, H., Kayed, R., Glabe, C.G., Saido, T.C., Iwata, N., Helms, J.B. & Yamaguchi, H. (2005). Oligomeric proteins ultrastructurally localize to cell processes, especially to axon terminals with higher density, but not to lipid rafts in Tg2576 mouse brain. Brain Research, 1045, (pp. 224-228) (5 p.).
Kokubo, H., Saido, T.C., Iwata, N., Helms, J.B., Shinohara, R. & Yamaguchi, H. (2005). Part of membrane-bound Abeta exists in rafts within senile plaques in Tg2576 mouse brain. Neurobiology of Aging, 4, (pp. 409-418) (10 p.).
  Articles - 2004
Bleijerveld, O.B., Klein, W., Vaandrager, A.B., Helms, J.B. & Houweling, M. (2004). Control of the CDPethanolamine pathway in mammalian cells: effect of CTP:phosphoethanolamine cytidylyltransferase overexpression and the amount of intracellular diacylglycerol. Biochemical Journal, 379 (3), (pp. 711-719) (9 p.).
Groves, M.R., Kuhn, A., Hendricks, A., Radke, S., Serrano, R.L., Helms, J.B. & Sinning, I. (2004). Crystallization of a Golgi-associated PR-1-related protein (GAPR-1) that localizes to lipid-enriched microdomains. Acta Crystallographica Section D: Biological Crystallography, 60 (4), (pp. 730-732) (3 p.).
van der Sanden, M.H.M., Meems, M.H., Houweling, M., Helms, J.B. & Vaandrager, A.B. (2004). Induction of CCAAT/Enhancer-binding protein (C/EBP)-homologous protein/growth arrest and DNA damage-inducible protein 153 expression during inhibition of phosphatidylcholine synthesis is mediated via activation of a C/EBP-activating transcription factor-responsive element. Journal of Biological Chemistry, 279 (50), (pp. 52007-52015) (9 p.).
Helms, J.B. & Zurzolo, C. (2004). Lipids as targeting signals: lipid rafts and intracellular trafficking. Traffic, 5 (4), (pp. 247-254) (8 p.).
Serrano, R.L., Kuhn, A., Hendricks, A., Helms, J.B., Sinning, I. & Groves, M.R. (2004). Structural analysis of the human Golgi-associated plant pathogenesis related protein GAPR-1 implicates dimerization as a regulatory mechanism. Journal of Molecular Biology, 339 (1), (pp. 173-183) (11 p.).
  Articles - 2003
da Silva, P.F.N., Brouwers, J.F.H.M., Helms, J.B., Colenbrander, B.A. & Gadella, B.M. (2003). Cholesterol oxidation is a common phenomenon in bovine sperm cells. Reproduction, 30, (pp. 87-88) (2 p.).
Stüven, E.P., Porat, A., Shimron, F., Fass, E., Kaloyanova, D.V., Brügger, B., Wieland, F.T., Elazar, Z. & Helms, J.B. (2003). Intra-Golgi protein transport depends on a cholesterol balance in the lipid membrane. Journal of Biological Chemistry, 278, (pp. 53112-53122) (11 p.).
Kokubo, H., Helms, J.B., Ohno-Iwashita, Y, Shimada, Y., Horikoshi, Y. & Yamaguchi, H. (2003). Ultrastructural localization of flotillin-1 to cholesterol-rich membrane microdomains, rafts, in rat brain tissue. Brain Research, 965, (pp. 83-90) (8 p.).
  Articles - 2002
Eberle, Heike B, Serrano, Ramon L, Füllekrug, Joachim, Schlosser, Andreas, Lehmann, Wolf D, Lottspeich, Friedrich, Kaloyanova, Dora, Wieland, Felix T & Helms, J Bernd (15.02.2002). Identification and characterization of a novel human plant pathogenesis-related protein that localizes to lipid-enriched microdomains in the Golgi complex. Journal of Cell Science, 115 (Pt 4), (pp. 827-38) (12 p.).
  Articles - 2001
Gommel, D U, Memon, A R, Heiss, A, Lottspeich, F, Pfannstiel, J, Lechner, J, Reinhard, C, Helms, J B, Nickel, W & Wieland, F T (03.12.2001). Recruitment to Golgi membranes of ADP-ribosylation factor 1 is mediated by the cytoplasmic domain of p23. EMBO Journal, 20 (23), (pp. 6751-60) (10 p.).
Gkantiragas, I, Brügger, B, Stüven, E, Kaloyanova, D, Li, X Y, Löhr, K, Lottspeich, F, Wieland, F T & Helms, J B (2001). Sphingomyelin-enriched microdomains at the Golgi complex. Molecular Biology of the Cell, 12 (6), (pp. 1819-33) (15 p.).
  Articles - 2000
Brügger, B, Sandhoff, R, Wegehingel, S, Gorgas, K, Malsam, J, Helms, J B, Lehmann, W D, Nickel, W & Wieland, F T (2000). Evidence for segregation of sphingomyelin and cholesterol during formation of COPI-coated vesicles. Journal of Cell Biology, 151 (3), (pp. 507-18) (12 p.).
Fischer, K D, Helms, J B, Zhao, L & Wieland, F T (2000). Site-specific photocrosslinking to probe interactions of Arf1 with proteins involved in budding of COPI vesicles. Methods in molecular medicine, 20 (4), (pp. 455-64) (10 p.). Copyright 2000 Academic Press..
  Articles - 1999
Zhao, L, Helms, J B, Brunner, J & Wieland, F T (1999). GTP-dependent binding of ADP-ribosylation factor to coatomer in close proximity to the binding site for dilysine retrieval motifs and p23. Journal of Biological Chemistry, 274 (20), (pp. 14198-203) (6 p.).
Gommel, D, Orci, L, Emig, E M, Hannah, M J, Ravazzola, M, Nickel, W, Helms, J B, Wieland, F T & Sohn, K (1999). p24 and p23, the major transmembrane proteins of COPI-coated transport vesicles, form hetero-oligomeric complexes and cycle between the organelles of the early secretory pathway. FEBS Letters, 447 (2-3), (pp. 179-85) (7 p.).
Reinhard, C, Harter, C, Bremser, M, Brügger, B, Sohn, K, Helms, J B & Wieland, F (16.02.1999). Receptor-induced polymerization of coatomer. Proceedings of the National Academy of Sciences of the United States of America, 96 (4), (pp. 1224-8) (5 p.).
  Articles - 1998
Helms, J B, Helms-Brons, D, Brügger, B, Gkantiragas, I, Eberle, H, Nickel, W, Nürnberg, B, Gerdes, H H & Wieland, F T (12.06.1998). A putative heterotrimeric G protein inhibits the fusion of COPI-coated vesicles. Segregation of heterotrimeric G proteins from COPI-coated vesicles. Journal of Biological Chemistry, 273 (24), (pp. 15203-8) (6 p.).
Nickel, W, Malsam, J, Gorgas, K, Ravazzola, M, Jenne, N, Helms, J B & Wieland, F T (1998). Uptake by COPI-coated vesicles of both anterograde and retrograde cargo is inhibited by GTPgammaS in vitro. Journal of Cell Science, 111 ( Pt 20), (pp. 3081-90) (10 p.).
  Articles - 1997
Zhao, L, Helms, J B, Brügger, B, Harter, C, Martoglio, B, Graf, R, Brunner, J & Wieland, F T (29.04.1997). Direct and GTP-dependent interaction of ADP ribosylation factor 1 with coatomer subunit beta. Proceedings of the National Academy of Sciences of the United States of America, 94 (9), (pp. 4418-23) (6 p.).
Galas, M C, Helms, J B, Vitale, N, Thiersé, D, Aunis, D & Bader, M F (31.01.1997). Regulated exocytosis in chromaffin cells. A potential role for a secretory granule-associated ARF6 protein. Journal of Biological Chemistry, 272 (5), (pp. 2788-93) (6 p.).
  Articles - 1996
Sohn, K, Orci, L, Ravazzola, M, Amherdt, M, Bremser, M, Lottspeich, F, Fiedler, K, Helms, J B & Wieland, F T (1996). A major transmembrane protein of Golgi-derived COPI-coated vesicles involved in coatomer binding. Journal of Cell Biology, 135 (5), (pp. 1239-48) (10 p.).
Nickel, W, Helms, J B, Kneusel, R E & Wieland, F T (05.07.1996). Forskolin stimulates detoxification of brefeldin A. Journal of Biological Chemistry, 271 (27), (pp. 15870-3) (4 p.).
  Articles - 1995
Helms, J B (01.08.1995). Role of heterotrimeric GTP binding proteins in vesicular protein transport - indications for both classical and alternative G protein cycles. FEBS Letters, 369 (1), (pp. 84-8) (5 p.).
  Articles - 1993
Palmer, D J, Helms, J B, Beckers, C J, Orci, L & Rothman, J E (05.06.1993). Binding of coatomer to Golgi membranes requires ADP-ribosylation factor. Journal of Biological Chemistry, 268 (16), (pp. 12083-9) (7 p.).
Tanigawa, G, Orci, L, Amherdt, M, Ravazzola, M, Helms, J B & Rothman, J E (1993). Hydrolysis of bound GTP by ARF protein triggers uncoating of Golgi-derived COP-coated vesicles. Journal of Cell Biology, 123 (6 Pt 1), (pp. 1365-71) (7 p.).
Helms, J B, Palmer, D J & Rothman, J E (1993). Two distinct populations of ARF bound to Golgi membranes. Journal of Cell Biology, 121 (4), (pp. 751-60) (10 p.).
  Articles - 1992
Helms, J B & Rothman, J E (26.11.1992). Inhibition by brefeldin A of a Golgi membrane enzyme that catalyses exchange of guanine nucleotide bound to ARF. Nature, 360 (6402), (pp. 352-4) (3 p.).
  Articles - 1991
Helms, J B, de Vries, K J & Wirtz, K W (15.11.1991). Synthesis of phosphatidylinositol 4,5-bisphosphate in the endoplasmic reticulum of Chinese hamster ovary cells. Journal of Biological Chemistry, 266 (32), (pp. 21368-74) (7 p.).
  Articles - 1990
Helms, J B, Karrenbauer, A, Wirtz, K W, Rothman, J E & Wieland, F T (15.11.1990). Reconstitution of steps in the constitutive secretory pathway in permeabilized cells. Secretion of glycosylated tripeptide and truncated sphingomyelin. Journal of Biological Chemistry, 265 (32), (pp. 20027-32) (6 p.).
Capitani, S, Helms, B, Mazzoni, M, Previati, M, Bertagnolo, V, Wirtz, K W & Manzoli, F A (1990). Uptake and phosphorylation of phosphatidylinositol by rat liver nuclei. Role of phosphatidylinositol transfer protein. Biochimica et Biophysica Acta, 1044 (2), (pp. 193-200) (8 p.).
  Articles - 1987
van Amerongen, A, Helms, J B, van der Krift, T P, Schutgens, R B & Wirtz, K W (02.06.1987). Purification of nonspecific lipid transfer protein (sterol carrier protein 2) from human liver and its deficiency in livers from patients with cerebro-hepato-renal (Zellweger) syndrome. Biochimica et Biophysica Acta, 919 (2), (pp. 149-55) (7 p.).
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Key research areas include:

(i) membrane dynamics in host – pathogen interactions, focusing on Salmonella typhimurium and on GAPR-1.

(ii) membrane dynamics of lipid droplets in normal and diseased liver  focusing on the role of hepatic stellate cells in liver regeneration.

(iii) membrane dynamics during fertilization, focusing on sperm and oocyte membrane dynamics during the fusion process.

(iv) the development and application of lipidomic techniques to fundamental, applied, and clinical research.

For a more detailed description, please visit the website of our research group:

www.bc-uu.nl

Gegenereerd op 2017-09-23 23:47:51
Additional functions and activities

No additional functions

Gegenereerd op 2017-09-23 23:47:51
Full name
prof. dr. J.B. Helms Contact details
Nieuw Gildestein

Yalelaan 2
Room 217
3584 CM  UTRECHT
The Netherlands


Phone number (direct) +31 30 253 5375
Phone number (department) +31 30 253 5389
Nieuw Gildestein

Bolognalaan 50 / Yalelaan 2 50
Room 217
3584 CJ  UTRECHT
The Netherlands


Phone number (direct) +31 30 253 5375
Phone number (department) +31 30 253 5389
Gegenereerd op 2017-09-23 23:47:51
Last updated 31.10.2016