Publications
2020
Scholarly publications
Lang, Y., Li, W., Li, Z., Koerhuis, D., van den Burg, A. C. S., Rozemuller, E., Bosch, B-J., van Kuppeveld, F. J. M., Boons, G-J., Huizinga, E. G., van der Schaar, H. M., & de Groot, R. J. (2020). Coronavirus hemagglutinin-esterase and spike proteins coevolve for functional balance and optimal virion avidity. Proceedings of the National Academy of Sciences of the United States of America, 117(41), 25759-25770. https://doi.org/10.1073/pnas.2006299117
2019
Scholarly publications
Vlieg, H. C., Huizinga, E. G., & Janssen, B. J. C. (2019). Structure and flexibility of the extracellular region of the PirB receptor. Journal of Biological Chemistry, 294(12), 4634-4643. https://doi.org/10.1074/jbc.RA118.004396
Hulswit, R. J. G., Lang, Y., Bakkers, M. J. G., Li, W., Li, Z., Schouten, A., Ophorst, B., van Kuppeveld, F. J. M., Boons, G-J., Bosch, B-J., Huizinga, E. G., & de Groot, R. J. (2019). Human coronaviruses OC43 and HKU1 bind to 9-O-acetylated sialic acids via a conserved receptor-binding site in spike protein domain A. Proceedings of the National Academy of Sciences of the United States of America, 116(7), 2681-2690. https://doi.org/10.1073/pnas.1809667116
2017
Scholarly publications
Sivapalaratnam, S., Westbury, S. K., Stephens, J. C., Greene, D., Downes, K., Kelly, A. M., Lentaigne, C., Astle, W. J., Huizinga, E. G., Nurden, P., Papadia, S., Peerlinck, K., Penkett, C. J., Perry, D. J., Roughley, C., Simeoni, I., Stirrups, K. E., Hart, D. P., Tait, R. C., ... Turro, E. (2017). Rare variants in GP1BB are responsible for autosomal dominant macrothrombocytopenia. Blood, 129(4), 520-524. https://doi.org/10.1182/blood-2016-08-732248
Bakkers, M. J. G., Lang, Y., Feitsma, L. J., Hulswit, R. J. G., de Poot, S. A. H., van Vliet, A. L. W., Margine, I., de Groot-Mijnes, J. D. F., van Kuppeveld, F. J. M., Langereis, M. A., Huizinga, E. G., & de Groot, R. J. (2017). Betacoronavirus Adaptation to Humans Involved Progressive Loss of Hemagglutinin-Esterase Lectin Activity. Cell Host and Microbe, 21(3), 356-366. https://doi.org/10.1016/j.chom.2017.02.008
2016
Scholarly publications
Bakkers, M. J. G., Zeng, Q., Feitsma, L. J., Hulswit, R. J. G., Li, Z., Westerbeke, A., van Kuppeveld, F. J. M., Boons, G-J., Langereis, M. A., Huizinga, E. G., & de Groot, R. J. (2016). Coronavirus receptor switch explained from the stereochemistry of protein-carbohydrate interactions and a single mutation. Proceedings of the National Academy of Sciences of the United States of America, 113(22), E3111-E3119. https://doi.org/10.1073/pnas.1519881113
2015
Scholarly publications
Koymans, K. J., Feitsma, L. J., Brondijk, T. H. C., Aerts, P. C., Lukkien, E., Lössl, P., van Kessel, K. P. M., de Haas, C. J. C., van Strijp, J. A. G., & Huizinga, E. G. (2015). Structural basis for inhibition of TLR2 by staphylococcal superantigen-like protein 3 (SSL3). Proceedings of the National Academy of Sciences of the United States of America, 112(35), 11018-23. [35]. https://doi.org/10.1073/pnas.1502026112
Diebolder, C. A., Halff, E. F., Koster, A. J., Huizinga, E. G., & Koning, R. I. (2015). Cryoelectron Tomography of the NAIP5/NLRC4 Inflammasome: Implications for NLR Activation. Structure, 23(12), 2349-57. https://doi.org/10.1016/j.str.2015.10.001
Langereis, M. A., Bakkers, M. J. G., Deng, L., Padler-Karavani, V., Vervoort, S. J., Hulswit, R. J. G., van Vliet, A. L. W., Gerwig, G. J., de Poot, S. A. H., Boot, W., van Ederen, A. M., Heesters, B. A., van der Loos, C. M., van Kuppeveld, F. J. M., Yu, H., Huizinga, E. G., Chen, X., Varki, A., Kamerling, J. P., & de Groot, R. J. (2015). Complexity and Diversity of the Mammalian Sialome Revealed by Nidovirus Virolectins. Cell Reports [E], 11(12), 1966-1978. https://doi.org/10.1016/j.celrep.2015.05.044
2014
Scholarly publications
Halff, E. F., Versteeg, M., Brondijk, T. H. C., & Huizinga, E. G. (2014). When less becomes more: Optimization of protein expression in HEK293-EBNA1 cells using plasmid titration - A case study for NLRs. Protein Expression and Purification, 99, 27-34. https://doi.org/10.1016/j.pep.2014.03.010
Volokina, E., Jakobi, A. J., Urbanus, R. T., Huizinga, E. G., Sluiter, H., Middel, C., Westra, D., van Heerde, W. L., van de Kar, N., & van den Heuvel, L. (2014). Novel sequence variation affects GPIb α in post-diarrheal hemolytic uremic syndrome. Journal of nephrology & therapeutics, S11, [007]. https://doi.org/10.4172/2161-0959.S11-007
2013
Scholarly publications
Karper, J. C., Ewing, M. M., de Vries, M. R., de Jager, S. C. A., Peters, E. A. B., de Boer, H. C., van Zonneveld, A. J., Kuiper, J., Huizinga, E. G., Brondijk, T. H. C., Jukema, J. W., & Quax, P. H. A. (2013). TLR accessory molecule RP105 (CD180) is involved in post-interventional vascular remodeling and soluble RP105 modulates neointima formation. PLoS One, 8(7), 1-9. [e67923]. https://doi.org/10.1371/journal.pone.0067923
2012
Scholarly publications
Volokhina, E. B., Jakobi, A. J., Huizinga, E. G., Sluiter, H. E., Middel, C., Westra, D., Van Heerde, W. L., Van De Kar, N. C. A. J., & Van Den Heuvel, L. P. (2012). Novel sequence variation affects GPIbα in typical HUS patient. Zoonoses and Public Health, 59, 55.
Halff, E. F., Diebolder, C. A., Versteeg, M., Schouten, A., Brondijk, T. H. C., & Huizinga, E. G. (2012). Formation and structure of a NAIP5-NLRC4 inflammasome induced by direct interactions with conserved N- and C-terminal regions of flagellin. Journal of Biological Chemistry, 287(46), 38460-38472. https://doi.org/10.1074/jbc.M112.393512
Langereis, M. A., Zeng, Q., Heesters, B., Huizinga, E. G., & de Groot, R. J. (2012). The murine coronavirus hemagglutinin-esterase receptor-binding site: a major shift in ligand specificity through modest changes in architecture. PLoS Pathogens, 8(1), 1-8. [e1002492]. https://doi.org/10.1371/journal.ppat.1002492
Brondijk, T. H. C., Bihan, D., Farndale, R. W., & Huizinga, E. G. (2012). Implications for collagen I chain registry from the structure of the collagen von Willebrand factor A3 domain complex. Proceedings of the National Academy of Sciences of the United States of America, 109(14), 5353-5258. https://doi.org/10.1073/pnas.1112388109
Bardoel, B. W., de Vos, R., Bouman, T., Aerts, P. C., Bestebroer, J., Huizinga, E. G., Brondijk, T. H. C., van Strijp, J. A. G., & de Gosselaar - de Haas, C. J. C. (2012). Evasion of Toll-like receptor 2 activation by staphylococcal superantigen-like protein 3. Journal of Molecular Medicine, 90(10), 1109-1120. http://www.ncbi.nlm.nih.gov/pubmed/22714643
Jakobi, A. J., & Huizinga, E. G. (2012). A rapid cloning method employing orthogonal end protection. PLoS One, 7(6), 1-9. [e37617]. https://doi.org/10.1371/journal.pone.0037617
2011
Scholarly publications
Jakobi, A. J., Mashaghi, A., Tans, S. J., & Huizinga, E. G. (2011). Calcium modulates force sensing by the von Willebrand factor A2 domain. Nature Communications [E], 2:385, 1-9. https://doi.org/10.1038/ncomms1385
2010
Scholarly publications
Brondijk, T. H. C., de Ruiter, T., Ballering, J., Wienk, H., Lebbink, R. J., van Ingen, H., Boelens, R., Farndale, R. W., Meyaard, L., & Huizinga, E. G. (2010). Crystal structure and collagen-binding site of immune inhibitory receptor LAIR-1: unexpected implications for collagen binding by platelet receptor GPVI. Blood, 115(7), 1364-1373. https://doi.org/10.1182/blood-2009-10-246322
van Wijk, R., Sutherland, S., van Wesel, A. C. W., Huizinga, E. G., Percy, M. J., Bierings, M. B., & Lee, F. S. (2010). Erythrocytosis associated with a novel missense mutation in the HIF2A gene. Haematologica-The Hematology Journal, 95(5), 829-832. https://doi.org/10.3324/haematol.2009.017582
2009
Scholarly publications
van Wijk, H. A., Huizinga, E. G., van Wesel, A. C. W., van Oirschot - Hermans, B. A., Hadders, M. A., & van Solinge, W. W. (2009). Fifteen novel mutations in PKLR associated with pyruvate kinase (PK) deficiency. Structural implications of amino acid substitutions in PK. Human Mutation, 30, 446-453.
Langereis, M. A., Zeng, Q., Gerwig, G. J., Frey, B., von Itzstein, M., Kamerling, J. P., de Groot, R. J., & Huizinga, E. G. (2009). Structural basis for ligand and substrate recognition by torovirus hemagglutinin esterases. Proceedings of the National Academy of Sciences of the United States of America, 106(37), 15897-15902. https://doi.org/10.1073/pnas.0904266106
Other output
Langereis, M. A., Zeng, A., Qinghong, G., Gerwig, G. J., Frey, B., von Itzstein, M., Kamerling, J. P., Johannis, P., de Groot, R. J., & Huizinga, E. G. (2009). Structural basis for receptor and substrate recognition by torovirus hemagglutinin-esterases. Poster session presented at FEBS-EMBO lecture course on Molecular and Cellular Membrane Biology, Cargèse, Corsica, France.
2008
Scholarly publications
Zeng, Q., Langereis, M. A., van Vliet, A. L., Huizinga, E. G., & de Groot, R. J. (2008). Structure of coronavirus hemagglutinin-esterase offers insight into corona and influenza virus evolution. Proceedings of the National Academy of Sciences of the United States of America, 105(26), 9065-9069. https://doi.org/10.1073/pnas.0800502105
Marx, P. F., Brondijk, T. H. C., Plug, T., Romijn, R. A., Hemrika, W., Meijers, J. C. M., & Huizinga, E. G. (2008). Crystal structures of TAFI elucidate the inactivation mechanism of activated TAFI: a novel mechanism for enzyme autoregulation. Blood, 112, 2803-2809.
https://dspace.library.uu.nl/bitstream/handle/1874/32221/28.pdf?sequence=1
https://dspace.library.uu.nl/bitstream/handle/1874/32221/28.pdf?sequence=1
2007
Scholarly publications
Milder, F. J., Gomes, L., Schouten, A., Janssen, B. J. C., Huizinga, E. G., Romijn, R. A., Hemrika, W., Roos, A., Daha, M. R., & Gros, P. (2007). Factor B structure provides insights into activation of the central protease of the complement system. Nature Structural and Molecular Biology, 14, 224-228.
https://dspace.library.uu.nl/bitstream/handle/1874/26877/08.pdf?sequence=1
https://dspace.library.uu.nl/bitstream/handle/1874/26877/08.pdf?sequence=1
2006
Scholarly publications
Staelens, S., Hadders, M. A., Vauterin, S., Platteau, C., De Maeyer, M., Vanhoorelbeke, K., Huizinga, E. G., & Deckmyn, H. (2006). Paratope Determination of the Antithrombotic Antibody 82D6A3 Based on the Crystal Structure of Its Complex with the von Willebrand Factor A3-Domain. Journal of Biological Chemistry, 281(4), 2225-2231. https://doi.org/10.1074/jbc.M508191200
Lisman, T., Raynal, N., Groeneveld, D., Maddox, B., Peachey, A. R., Huizinga, E. G., de Groot, P. G., & Farndale, R. W. (2006). A single high-affinity binding site for von Willebrand factor in collagen III, identified using synthetic triple-helical peptides. Blood, 108, 3753-3756.
https://dspace.library.uu.nl/bitstream/handle/1874/19339/10.1182-blood-2006-03-011965.pdf?sequence=1
https://dspace.library.uu.nl/bitstream/handle/1874/19339/10.1182-blood-2006-03-011965.pdf?sequence=1
Milder, F. J., Raaijmakers, H. C. A., Vandeputte, D. A. A., Schouten, A., Huizinga, E. G., Romijn, R. A., Hemrika, W., Roos, A., Daha, M. R., & Gros, P. (2006). Structure of complement component C2a: implications for convertase formation and substrate binding. Structure, 14(10), 1587-1597.
https://dspace.library.uu.nl/bitstream/handle/1874/20068/10.1016-j.str.2006.08.008.pdf?sequence=1
https://dspace.library.uu.nl/bitstream/handle/1874/20068/10.1016-j.str.2006.08.008.pdf?sequence=1
Obert, B., Romijn, R. A. P., Houllier, A., Huizinga, E. G., & Girma, J. P. (2006). Characterization of bitiscetin-2, a second form of bitiscetin from the venom of Bitis Arietans: comparison of its binding site with the collagen-binding site on the von Willebrand Factor A3-domain. Journal of Thrombosis and Haemostasis, 4, 1596-1601.
https://dspace.library.uu.nl/bitstream/handle/1874/19465/10.1111-j.1538-7836.2006.01994.x.pdf?sequence=1
https://dspace.library.uu.nl/bitstream/handle/1874/19465/10.1111-j.1538-7836.2006.01994.x.pdf?sequence=1
2005
Scholarly publications
Janssen, B. J. C., Huizinga, E. G., Raaijmakers, H. C. A., Roos, A., Daha, M. R., Nilsson-Ekdahl, K., Nilsson, B., & Gros, P. (2005). Structures of complement component C3 provide insights into the function and evolution of immunity. Nature, 437, 505-511.
https://dspace.library.uu.nl/bitstream/handle/1874/14832/nature04005.pdf?sequence=2
https://dspace.library.uu.nl/bitstream/handle/1874/14832/nature04005.pdf?sequence=2
2004
Scholarly publications
Lenting, P. J., Westein, E., Terraube, V., Ribba, A-S., Huizinga, E. G., Meyer, D., de Groot, P. G., & Denis, C. V. (2004). An experimental model to study in vivo survival of Von Willebrand factor - Basic aspects and application to the R1205H mutation. Journal of Biological Chemistry, 279, 12102-12109.
van Wijk, R., Huizinga, E. G., Prins, I., Kors, W. A., Rijksen, G., Bierings, M. B., & van Solinge, W. W. (2004). Distinct phenotypic expression of two de novo missense mutations affecting the dimer interface of glucose-6-phosphate. Blood Cells, Molecules, & Diseases, 32, 112-117.
Vanhoorelbeke, K., Ulrichts, H., Romijn, R. A. P., Huizinga, E. G., & Deckmyn, H. (2004). The GPlbα-thrombin interaction: far from crystal clear. Trends in Molecular Medicine, 10(1), 33-39.
https://dspace.library.uu.nl/bitstream/handle/1874/14596/10.1016-j.molmed.2003.11.009.pdf?sequence=1
https://dspace.library.uu.nl/bitstream/handle/1874/14596/10.1016-j.molmed.2003.11.009.pdf?sequence=1
2003
Scholarly publications
Vanhoorelbeke, K., Depraetere, H., Romijn, R. A. P., Huizinga, E. G., Maeyeri, M. D., & Deckmyn, H. (2003). A Consensus Tetrapeptide Selected by Phage Display Adopts the Conformation of a Dominant Discontinuous Epitope of a Monoclonal Anti-VWF Antibody That Inhibits the von Willebrand Factor-Collagen Interaction. Journal of Biological Chemistry, 278(39), 37815-37821.
https://dspace.library.uu.nl/bitstream/handle/1874/14402/10.1074-jbc.M304289200.pdf?sequence=1
https://dspace.library.uu.nl/bitstream/handle/1874/14402/10.1074-jbc.M304289200.pdf?sequence=1
van Wijk, H. A., Rijksen, G., Huizinga, E. G., Nieuwenhuis, H. K., & van Solinge, W. W. (2003). HK Utrecht: missense mutation in the active site of human hexokinase associated with hexokinase deficiency and severe nonspherocytic hemolytic anemia. Blood, 101, 345-347.
https://dspace.library.uu.nl/bitstream/handle/1874/14148/10.1182-blood-2002-06-1851.pdf?sequence=1
https://dspace.library.uu.nl/bitstream/handle/1874/14148/10.1182-blood-2002-06-1851.pdf?sequence=1
Tsuji, S., & Huizinga, E. G. (2003). Mechanism of the Glycoprotein Iba-von Willebrand Factor interaction based on crystal structure analysis. Japanese Journal of Thrombosis and Hemostasis, 14, 101-106.
Romijn, R. A. P., Westein, E., Bouma, B., Schiphorst, M. E., Sixma, J. J., Lenting, P. J., & Huizinga, E. G. (2003). Mapping the collagen-binding site in the von Willebrand Factor-A3 domain. Journal of Biological Chemistry, 278(17), 15035-15039.
https://dspace.library.uu.nl/bitstream/handle/1874/14401/10.1074-jbc.M208977200.pdf?sequence=1
https://dspace.library.uu.nl/bitstream/handle/1874/14401/10.1074-jbc.M208977200.pdf?sequence=1
2002
Scholarly publications
Oubrie, A., Rozeboom, H. J., Kalk, K. H., Huizinga, E. G., & Dijkstra, B. W. (2002). Crystal structure of quinohemoprotein alcohol dehydrogenase from Comamonas testosteroni: Structural basis for substrate oxidation and electron transfer. Journal of Biological Chemistry, 277(5), 3727-3732. https://doi.org/10.1074/jbc.M109403200
Huizinga, E. G., Tsuji, S., Romijn, R. A. P., Schiphorst, M. E., de Groot, P. G., Sixma, J. J., & Gros, P. (2002). Structures of glycoprotein Ibα and its complex with von Willebrand Factor A1 domain. Science, 297, 1176-1179.
https://dspace.library.uu.nl/bitstream/handle/1874/14142/Huizinga_p1176-1179_02.pdf?sequence=1
https://dspace.library.uu.nl/bitstream/handle/1874/14142/Huizinga_p1176-1179_02.pdf?sequence=1
2001
Scholarly publications
Huizinga, E. G., Schouten, A., Connolly, T. M., Kroon, J., Sixma, J. J., & Gros, P. (2001). The structure of leech anti-platelet protein, an inhibitor of haemostasis. Acta Crystallographica Section D: Biological Crystallography, 57(8), 1071-1078. https://doi.org/10.1107/S0907444901007405
Cauwenberghs, N., Vanhoorelbeke, K., Vauterin, S., Westra, D. F., Romo, G., Huizinga, E. G., Lopez, J. A., Berndt, M. C., Harsfalvi, J., & Deckmyn, H. (2001). Epitope mapping of inhibitory antibodies against platelet glycoprotein Ibα reveals interaction between the leucine-rich repeat N-terminal and C-terminal flanking domains of glycoprotein Ibα. Blood, 98(3), 652-660. https://doi.org/10.1182/blood.V98.3.652
Oubrie, A., Huizinga, E. G., Rozeboom, H. J., Kalk, K. H., De Jong, G. A. H., Duine, J. A., & Dijkstra, B. W. (2001). Crystallization of quinohaemoprotein alcohol dehydrogenase from Comamonas testosteroni: Crystals with unique optical properties. Acta crystallographica. Section D, biological crystallography, 57(11), 1732-1734. https://doi.org/10.1107/S0907444901013002
Romijn, R. A. P., Bouma, B., Wuyster, W., Gros, P., Kroon, J., Sixma, J. J., & Huizinga, E. G. (2001). Identification of the collagen-binding site of the von Willebrand Factor A3-domain. Journal of Biological Chemistry, 276, 9985-9991.
van Wijk, R., Nieuwenhuis, K., van den Berg, H. M., Huizinga, E. G., van der Meijden, B. B., Kraaijenhagen, R. J., & van Solinge, W. W. (2001). Five novel mutations in the gene for human blood coagulation factor V associated with type I factor V deficiency. Blood, 98(2), 358-365.
Huizinga, E. G., Schouten, A., Connolly, T. M., Kroon, J., Sixma, J. J., & Gros, P. (2001). The structure of leech anti-plated protein, an inhibitor of haemostasis. Acta crystallographica. Section D, biological crystallography, D 57, 1071-1078.
2000
Scholarly publications
Van Der Plas, R. M., Gomes, L., Marquart, J. A., Vink, T., Meijers, J. C. M., De, G. G., Sixma, J. J., & Huizinga, E. G. (2000). Binding of von Willebrand factor to collagen type III: Role of specific amino acids in the collagen binding domain of vWF and effects of neighboring domains. Thrombosis and Haemostasis, 84(6), 1005-1011.
Vanhoorelbeke, K., van der Plas, R. M., Vandecasteele, G., Vauterin, S., Huizinga, E. G., Sixma, J. J., & Deckmijn, H. (2000). Sequence alignment between vWF and peptides inhibiting the vWF-collagen interaction does not result in the identification of a collagen-binding site in vWF. Thrombosis and Haemostasis, 84, 621-625.
1999
Scholarly publications
Van Der Plas, R. M., Schiphorst, M. E., Huizinga, E. G., Hené, R. J., Verdonck, L. F., Sixma, J. J., & Fijnheer, R. (1999). von Willebrand factor proteolysis is deficient in classic, but not in bone marrow transplantation-associated, thrombotic thrombocytopenic purpura. Blood, 93(11), 3798-3802.
Verkley, M. W., IJsseldijk, M. J. W., Heijnen-Snyder, G. J., Huizinga, E. G., Morton, L. F., Knight, C. G., Sixma, J. J., de Groot, P. G., & Barnes, M. J. (1999). Adhesive domains in the collagen III fragment a1 (III)CB4 that support a2b1- and von Willebrand factor-mediated platelet adhesion under flow conditions. Thrombosis and Haemostasis, 82, 1137-1144.
Other output
Rump, E. T., Rijkers, D. T. S., Huizinga, E. G., Liskamp, R. M. J., & de Groot, P. G. (1999). Synthesis and analysis of collagen structures. Abstract from Biomed. Meeting Debrecen, Hongarije.
1997
Scholarly publications
Sixma, J. J., van Zanten, G. H., Huizinga, E. G., van der Plas, R. M., Verkleij, M., Wu, Y. P., Gros, P., & de Groot, P. G. (1997). Platelet adhesion to collagen: an update. Thrombosis and Haemostasis, 78, 434-438.
Huizinga, E. G., van der Plas, R. M., Kroon, J., Sixma, J. J., & Gros, P. (1997). Crystal structure of the A3 domain of human von Willebrand factor: implications for collagen binding. Structure, 5, 1147-1156.
1992
Scholarly publications
Chen, L., Mathews, F. S., Davidson, V. L., Huizinga, E. G., Vellieux, F. M. D., & Hol, W. G. J. (1992). Three-dimensional structure of the quinoprotein methylamine dehydrogenase from Paracoccus denitrificans determined by molecular replacement at 2.8 Å resolution. Proteins: Structure function and bioinformatics, 14(2), 288-299.
Huizinga, E. G., Van Zanten, B. A. M., Duine, J. A., Jongejan, J. A., Huitema, F., Wilson, K. S., & Hol, W. G. J. (1992). Active site structure of methylamine dehydrogenase: Hydrazines identify C6 as the reactive site of the tryptophan-derived quinone cofactor. Biochemistry, 31(40), 9789-9795. https://doi.org/10.1021/bi00155a036
1991
Scholarly publications
Chen, L., Mathews, F. S., Davidson, V. L., Huizinga, E. G., Vellieux, F. M. D., Duine, J. A., & Hol, W. G. J. (1991). Crystallographic investigations of the tryptophan-derived cofactor in the quinoprotein methylamine dehydrogenase. FEBS Letters, 287(1-2), 163-166. https://doi.org/10.1016/0014-5793(91)80041-Z