Publications
2023
Scholarly publications
Chen, X., Moran Torres, J. P., Li, Y., Lugones, L. G., & Wösten, H. A. B. (2023). Inheritable CRISPR based epigenetic modification in a fungus. Microbiological Research, 272, [127397]. https://doi.org/10.1016/j.micres.2023.127397
Lyu, J., Tegelaar, M., Post, H., Moran Torres, J., Torchia, C., Altelaar, A. F. M., Bleichrodt, R-J., de Cock, H., Lugones, L. G., & Wösten, H. A. B. (2023). Heterogeneity in Spore Aggregation and Germination Results in Different Sized, Cooperative Microcolonies in an Aspergillus niger Culture. mBio, 14(1), 1-20. [e0087022]. https://doi.org/10.1128/mbio.00870-22
2022
Scholarly publications
Marian, I. M., Vonk, P. J., Valdes, I. D., Barry, K., Bostock, B., Carver, A., Daum, C., Lerner, H., Lipzen, A., Park, H., Schuller, M. B. P., Tegelaar, M., Tritt, A., Schmutz, J., Grimwood, J., Lugones, L. G., Choi, I-G., Wösten, H. A. B., Grigoriev, I. V., & Ohm, R. A. (2022). The Transcription Factor Roc1 Is a Key Regulator of Cellulose Degradation in the Wood-Decaying Mushroom Schizophyllum commune. mBio, 13(3), 1-19. [e0062822]. https://doi.org/10.1128/mbio.00628-22
2021
Scholarly publications
Dalonso, N., Petkowicz, C. L. O., Lugones, L. G., Silveira, M. L. L., & Gern, R. M. M. (2021). Comparison of cell wall polysaccharides in schizophyllum commune after changing phenotype by mutation. Anais da Academia Brasileira de Ciencias, 93, 1-17. [e20210047]. https://doi.org/10.1590/0001-3765202120210047
Vos, A. M., Bleichrodt, R-J., Herman, K. C., Ohm, R. A., Scholtmeijer, K., Schmitt, H., Lugones, L. G., & Wösten, H. A. B. (2021). Cycling in degradation of organic polymers and uptake of nutrients by a litter-degrading fungus. Environmental Microbiology, 23(1), 224-238. https://doi.org/10.1111/1462-2920.15297
2019
Scholarly publications
Jan Vonk, P., Escobar, N., Wösten, H. A. B., Lugones, L. G., & Ohm, R. A. (2019). High-throughput targeted gene deletion in the model mushroom Schizophyllum commune using pre-assembled Cas9 ribonucleoproteins. Scientific Reports, 9(1), 1-8. [7632]. https://doi.org/10.1038/s41598-019-44133-2
2018
Scholarly publications
Vos, A. M., Jurak, E., de Gijsel, P., Ohm, R. A., Henrissat, B., Lugones, L. G., Kabel, M. A., & Wösten, H. A. B. (2018). Production of α-1,3-L-arabinofuranosidase active on substituted xylan does not improve compost degradation by Agaricus bisporus. PLoS One, 13(7), 1-12. [e0201090]. https://doi.org/10.1371/journal.pone.0201090
2017
Scholarly publications
Vos, A., Jurak, E., Pelkmans, J., Herman, K., Pels, G., Baars, J. J., Hendrix, E., Kabel, M. A., Lugones, L. G., & Wosten, H. (2017). H2O2 as a candidate bottleneck for MnP activity during cultivation of Agaricus bisporus in compost. AMB Express, 7(1), 1-9. [124]. https://doi.org/10.1186/s13568-017-0424-z
Pelkmans, J. F., Patil, M. B., Gehrmann, T., Reinders, M. J. T., Wösten, H. A. B., & Lugones, L. G. (2017). Transcription factors of Schizophyllum commune involved in mushroom formation and modulation of vegetative growth. Scientific Reports, 7(1), 1-11. [310]. https://doi.org/10.1038/s41598-017-00483-3
Vos, A., Heijboer, A., Boschker, H. T. S., Bonnet, B., Lugones, L., & Wosten, H. (2017). Microbial biomass in compost during colonization of Agaricus bisporus. AMB Express, 7, 1-7. [12]. https://doi.org/10.1186/s13568-016-0304-y
2016
Scholarly publications
Pelkmans, J. F., Vos, A. M., Scholtmeijer, K., Hendrix, E., Baars, J. J., Gehrmann, T., Reinders, M. J., Lugones, L. G., & Wösten, HAB. (2016). The transcriptional regulator c2h2 accelerates mushroom formation in Agaricus bisporus. Applied Microbiology and Biotechnology, 100(16), 7151-7159. https://doi.org/10.1007/s00253-016-7574-9
Gehrmann, T., Pelkmans, J. F., Lugones, L. G., Wösten, HAB., Abeel, T., & Reinders, M. J. (2016). Schizophyllum commune has an extensive and functional alternative splicing repertoire. Scientific Reports, 6, 1-11. [33640]. https://doi.org/10.1038/srep33640
https://dspace.library.uu.nl/bitstream/handle/1874/345040/Schizophyllum.pdf?sequence=1
https://dspace.library.uu.nl/bitstream/handle/1874/345040/Schizophyllum.pdf?sequence=1
Pelkmans, J. F., Lugones, L. G., & Wosten, H. A. B. (2016). Fruiting Body Formation in Basidiomycetes. In The Mycota: Growth, Differentiation and Sexuality (3rd ed., Vol. 1, pp. 387-405) https://doi.org/10.1007/978-3-319-25844-7_15
https://dspace.library.uu.nl/bitstream/handle/1874/330405/chp_3A10.1007_2F978_3_319_25844_7_15.pdf?sequence=1
https://dspace.library.uu.nl/bitstream/handle/1874/330405/chp_3A10.1007_2F978_3_319_25844_7_15.pdf?sequence=1
van Leeuwen, M. R., Wyatt, T. T., van Doorn, T. M., Lugones, L. G., Wosten, H., & Dijksterhuis, J. (2016). Hydrophilins in the filamentous fungus Neosartorya fischeri (Aspergillus fischeri) have protective activity against several types of microbial water stress. Environmental Microbiology Reports, 8(1), 45-52. https://doi.org/10.1111/1758-2229.12349
2014
Scholarly publications
Scholtmeijer, K., Cankar, K., Beekwilder, J., Lugones, L. G., Bosch, D., & Wosten, H. (2014). Production of (+)-valencene in the mushroom-forming fungus S. commune. Applied Microbiology and Biotechnology, 98(11), 5059-5068. https://doi.org/10.1007/s00253-014-5581-2
Other output
Wösten, H. A. B., Lugones, L. G., Pelkmans, J. F., & Wiegers, I. E. H. (2014). DST2 of Schizophyllum commune is involved in the transition of primordia into mature fruiting bodies. 202. Poster session presented at 12th European Conference on Fungal Genetics, Sevilla.
Vos, A. M., Wösten, H. A. B., & Lugones, L. G. (2014). The effect of hydrophobins on water repellency of soil and enzyme activity. 314. Poster session presented at 12th European Conference on Fungal Genetics, Sevilla.
2013
Scholarly publications
Berends, E., Lehle, L., Henquet, M., Hesselink, T., Wösten, H. A. B., Lugones, L. G., & Bosch, D. (2013). Identification of alg3 in the mushroom-forming fungus Schizophyllum commune and analysis of the Δalg3 knockout mutant. Glycobiology, 23(2), 147-154. https://doi.org/10.1093/glycob/cws135
Berendsen, R. L., Kalkhove, S. I. C., Lugones, L. G., Baars, J. J., Wösten, H. A. B., & Bakker, P. A. H. M. (2013). Effects of the mushroom-volatile 1-octen-3-ol on dry bubble disease. Applied Microbiology and Biotechnology, 97(12), 5535-5543.
Berends, E., Lehle, L., Henquet, M., Hesselink, T., Wösten, H. A. B., Lugones, L. G., & Bosch, D. (2013). Identification of alg3 in the mushroom-forming fungus Schizophyllum commune and analysis of the delta-alg3 knockout mutant. Glycobiology, 23(2), 147-154.
Berendsen, R. L., Schrier, N., Kalkhove, S. I. C., Lugones, L. G., Baars, J. J. P., Zijlstra, C., de Weerdt, M., Wösten, H. A. B., & Bakker, P. A. H. M. (2013). Absence of induced resistance in Agaricus bisporus against Lecanicillium fungicola. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, 103, 539-550. https://doi.org/10.1007/s10482-012-9836-6
Ohm, R. A., Aerts, D., Wösten, H. A. B., & Lugones, L. G. (2013). The blue light receptor complex WC-1/2 of Schizophyllum commune is involved in mushroom formation and protection against phototoxicity. Environmental Microbiology, 15(3), 943-955.
2012
Scholarly publications
Berendsen, R. L., Kalkhove, S. I. C., Lugones, L. G., Baars, J. J. P., & Wösten, H. A. B. (2012). Effects of fluorescent Pseudomonas spp. isolated from mushroom cultures on Lecanicillium fungicola. Biological Control, 63(2), 210-221. https://doi.org/10.1016/j.biocontrol.2012.07.012
Berendsen, R. L., Kalkhove, S. I. C., Lugones, L. G., Wösten, H. A. B., & Bakker, P. A. H. M. (2012). Germination of Lecanicillium fungicola in the mycosphere of Agaricus bisporus. Environmental Microbiology Reports, 4(2), 227-233. https://doi.org/10.1111/j.1758-2229.2011.00325.x
Other output
Ohm, R. A., Aerts, D., Lugones, L. G., & Wösten, H. A. B. (2012). The WC1/WC2 complex is the fructification master switch in Schizophyllum commune. Abstract from The 18th Congress of the International Society for Mushroom Science, Beijng, China.
2011
Scholarly publications
Berends, E., Schmidt, K., Lugones, L., Wosten, H., & Bosch, D. (2011). Mushroom glycoengineering for the production of therapeutic glycoproteins. Glycoconjugate Journal, 28(5), 239-240.
Ohm, R. A., de Jong, J. F., de Bekker, A. M., Wösten, H. A. B., & Lugones, L. G. (2011). Transcription factor genes of Schizophyllum commune involved in regulation of mushroom formation. Molecular Microbiology, 81(6), 1433-1445. https://doi.org/10.1111/j.1365-2958
Other output
Bleichrodt, R., van Peer, A. F., Recter, B., Muller, W. H., Boekhout, T., Maruyama, J-I., Kitamoto, K., Lugones, L. G., & Wösten, H. A. B. (2011). Septal plugging is a dynamic process which depends on the environmental conditions. Abstract from 26th Fungal Genetics Conference Asilomar 2011, Asilomar, USA.
Ohm, R. A., de Jong, J. F., Lugones, L. G., & Wösten, H. A. B. (2011). Regulation of mushroom development. Abstract from 26th Fungal Genetics Conference Asilomar 2011, Asilomar, USA.
Hu, Z., Wösten, H. A. B., & Lugones, L. G. (2011). Optimization of transformation of the mushroom Agaricus bisporus. Poster session presented at Voorjaarsvergadering van de Nederlandse Vereniging voor Medische Microbiologie (NVMM) en de Nederlandse Vereniging voor Microbiologie (NVvM), Papendal (Arnhem).
Berendsen, R. L., Kalkhove, S. I. C., Lugones, L. G., Wösten, H. A. B., & Bakker, P. A. H. M. (2011). Control and ecology of dry bubble disease of the white button mushrooms. Paper presented at EPS automn school Host-microbe interactions, Wageningen.
Hu, Z., Wösten, H. A. B., & Lugones, L. G. (2011). Optimization of transformation of the mushroom Agaricus bisporus. Abstract from Voorjaarsvergadering van de Nederlandse Vereniging voor Medische Microbiologie (NVMM) en de Nederlandse Vereniging voor Microbiologie (NVvM), Papendal (Arnhem).
2010
Scholarly publications
Ohm, R. A., De Jong, J. F., Lugones, L. G., Aerts, A., Kothe, E., Stajich, J. E., De Vries, R. P., Record, E., Levasseur, A., Baker, S. E., Bartholomew, K. A., Coutinho, P. M., Erdmann, S., Fowler, T. J., Gathman, A. C., Lombard, V., Henrissat, B., Knabe, N., Kües, U., ... Wösten, H. A. B. (2010). Genome sequence of the model mushroom Schizophyllum commune. Nature Biotechnology, 28(9), 957-963. https://doi.org/10.1038/nbt.1643
Ohm, R. A., de Jong, J. F., Lugones, L. G., Aerts, A., Kothe, E., Stajich, J. E., de Vries, R. P., Record, E., Levasseur, A., Baker, S. E., Bartolomew, K. A., Coutinho, P. M., Erdmann, S., Fowler, T. J., Gathman, A. C., Lombard, V., Henrissat, B., Knabe, N., Kües, U., ... Wösten, H. A. B. (2010). Genome sequence of the model mushroom Schizophillum commune. Nature Biotechnology, 28(9), 957-963.
de Jong, J. F., Ohm, R. A., de Bekker, A. M., Wösten, H. A. B., & Lugones, L. G. (2010). Inactivation of ku80 in the mushroom-forming fungus Schizophyllum commune increases the relative incidence of homologous recombination. FEMS Microbiology Letters, 310(1), 91-95.
Berendsen, R. L., Baars, J. J. P., Kalkhove, S. I. C., Lugones, L. G., Wösten, H. A. B., & Bakker, P. A. H. M. (2010). Pathogen profile: Lecanicillium fungicola, causal agent of dry bubble disease in white-button mushroom. Molecular Plant Pathology, 11, 585-595. https://doi.org/10.1111/j.1364-3703.2010.00627.x
van Peer, A. F., Wang, F., van Driel, K. G. A., de Jong, J. F., van Donselaar, E. G., Muller, W. H., Boekhout, T., Lugones, L. G., & Wösten, H. A. B. (2010). The septal pore cap is an organelle that functions in vegetative growth and mushroom formation of the wood-rot fungus Schizophyllum commune. Environmental Microbiology, 12(4), 833-844.
Ohm, R. A., de Jong, J. F., Berends, E., Wang, F., Wösten, H. A. B., & Lugones, L. G. (2010). An efficient gene deletion procedure for the mushroom-forming basidiomycete Schizophyllum commune. World Journal of Microbiology and Biotechnology.
Other output
Ohm, R. A., de Jong, J. F., Lugones, L. G., & Wösten, H. A. B. (2010). Regulatiors involved in mushroom formation. (Patent No. PCT/NL2010/050206).
Berendsen, R. L., Kalkhove, S. I. C., Baars, J. J. P., Lugones, L. G., Wosten, H. A. B., & Bakker, P. A. H. M. (2010). Microbial inhibition of Lecanicillium fungicola in thr mycosphere of Agaricus bisporus.. Paper presented at 13th International Symposium on Microbial Ecology, Seattle, USA.
Ohm, R. A., de Jong, J. F., Lugones, L. G., & Wösten, H. A. B. (2010). Genome of Schizophyllum commune yields insight into regulation of mushroom formation. Abstract from 10th European Conference on Fungal Genetics, Noordwijkerhout (NH Conference Center Leeuwenhorst).
Ohm, R. A., de Jong, J. F., Lugones, L. G., & Wösten, H. A. B. (2010). Genome of Schizophyllum commune yields insight into regulation of mushroom formation. Poster session presented at 10th European Conference on Fungal Genetics, Noordwijkerhout (NH Conference Center Leeuwenhorst).
2009
Scholarly publications
Van Peer, A. F., De Bekker, C., Vinck, A., Wösten, H. A. B., & Lugones, L. G. (2009). Phleomycin increases transformation efficiency and promotes single integrations in schizophyllum commune. Applied and Environmental Microbiology, 75(5), 1243-1247. https://doi.org/10.1128/AEM.02162-08
Berendsen, R. L., Baars, J., Kalkhove, S., Wadman, M. W., Lugones, L. G., Wösten, H. A. B., & Bakker, P. A. H. M. (2009). Selection of Pseudomonas strains that are antagonistic to Verticillium fungicola, the causal agent of dry bubble disease in Agaricus bisporus. In B. Duffy, M. Maurhofer, C. Keel, C. Gessler, Y. Elad, & S. Kiewnick (Eds.), Molecular tools for understanding and improving biocontrol. (pp. 32). (WPRS Bulletin; No. 43). IOBC.
Berends, E., Scholtmeijer, K., Wösten, H. A. B., Bosch, D., & Lugones, L. G. (2009). The use of mushroom-forming fungi for the production of N-glycosylated therapeutic proteins. Trends in Microbiology, 17(10), 439-443.
van Peer, A. F., Muller, W. H., Boekhout, T., Lugones, L. G., & Wösten, H. A. B. (2009). Cytoplasmic continuity revisited: closure of septa of the filamentous fungus Schizophyllum commune in response to environmental conditions. PLoS One, 4(6), 1-5. [e5977]. https://doi.org/10.1371/journal.pone.0005977
van Peer, A. F., de Bekker, A. M., Vinck, A., Wösten, H. A. B., & Lugones, L. G. (2009). Pleomycin increases transformation efficiency and promotes single integrations in Schizophyllum commune. Applied Environmental Microbiology, 75(5), 1243-1247.
Berends, E., Ohm, R. A., de Jong, J. F., Rouwendal, G. J. A., Wösten, H. A. B., Lugones, L. G., & Bosch, D. (2009). Genomic and biochemical analysis of N glycosylation in the mushroom-forming basidiomycete Schizophyllum commune. Applied and Environmental Microbiology, 75(13), 4648-4652.
Other output
Berendsen, R., Baars, J., Kalkhove, S., Wadman, M. W., Lugones, L. G., Wösten, H. A. B., & Bakker, P. A. H. M. (2009). Selection of Pseudomonas strains that are antagonistic to Lecanicillium fungicola, the causal agent of dry bubble disease in Agaricus bisporus.. 59. Abstract from 8th International PGPR Workshop, Portland, Oregon, USA.
2008
Other output
Ohm, R. A., Sharif, S. N., Huijzer, T. C. D., Quax, T. E. F., Leeuwen, J. G. E., de Jong, J. F., Wösten, H. A. B., & Lugones, L. G. (2008). Localization of gene expression in developmental stages of Schizophyllum commune using the fluorescent marker dTomato. Abstract from Genetics and Cell Biology of Basidiomycetes Conference VII, Cape Girardeau, MO.
de Jong, J. F., Wadman, M. W., Leeuwen, J. G. E., Lugones, L. G., & Wösten, H. A. B. (2008). Birch wood volatiles induce a chemotropic response in Schizophyllum commune. Abstract from Genetics and Cell Biology of Basidiomycetes Conference VII, Cape Girardeau, MO.
2006
Scholarly publications
de Jong, J. F., Deelstra, H. J., Wösten, H. A. B., & Lugones, L. G. (2006). RNA-mediated gene silencing in monokaryons and dikaryons of Schizophyllum commune. Applied and Environmental Microbiology, 72, 1267-1269.
Other output
Alves, A. M., Lugones, L. G., & Wösten, H. A. B. (2006). Expression of monokaryon and dikaryon-specific promoters of Schizophyllum commune in Pycnoporus cinnabarinus. Poster session presented at 8th European Conference on Fungal Genetics, Vienna, Austria.
2005
Scholarly publications
Dijksterhuis, J., Samson, R., Wösten, H. A. B., & Lugones, L. G. (2005). Play, an abundant ascopore cell wall protein in Talaromyces macrosporus. Nederlands Tijdschrift voor Medische Microbiologie, 57.
2004
Scholarly publications
Lugones, L. G., de Jong, J. F., de Vries, O. M. H., Jalving, R., Dijksterhuis, J., & Wösten, H. A. B. (2004). The Sc15 protein of Schizophyllum commune mediates formation of aerial hyphae and attachment in the absence of the SC3 hydrophobin. Molecular Microbiology, 53, 707-716.
Teertstra, W. R., Lugones, L. G., & Wösten, H. A. B. (2004). In situ hybridisation in filamentous fungi using peptide nucleic acid probes. Fungal Genetics and Biology, 41, 1099-1103.
Other output
de Jong, J. F., de Vries, O. M. H., Jalving, R., Dijksterhuis, J., Wösten, H. A. B., Wessels, J. G. H., & Lugones, L. G. (2004). SC15 of Schizophyllum commune mediates formation of aerial hyphae and attachment to hydrophobic surfaces in the absence of SC3. Abstract from 7th European Conference on Fungal Genetics Copenhagen, Kopenhagen.
2003
Other output
de Jong, J. F., Wösten, H. A. B., & Lugones, L. G. (2003). The role of SC15 in the formation of aerial hyphae in Schizophyllum commune. Abstract from Voorjaarsvergadering NVMM/NVvM, Papendal.
1999
Scholarly publications
Lugones, L. G., Wösten, H. A. B., Birkenkamp, K. U., Sjollema, K. A., Zagers, J., & Wessels, J. G. H. (1999). Hydrophobins line air channels in fruiting bodies of Schizophyllum commune and Agaricus bisporus. Mycological Research, 103(5), 635-640. https://doi.org/10.1017/S0953756298007552
Wosten, H., van Wetter, M. A., Lugones, L. G., van der Mei, H. C., Busscher, H. J., & Wessels, J. G. (1999). How a fungus escapes the water to grow into the air. Current Biology, 9(2), 85-8.
1998
Scholarly publications
Lugones, L. G., Wosten, H., & Wessels, J. G. (1998). A hydrophobin (ABH3) specifically secreted by vegetatively growing hyphae of Agaricus bisporus (common white button mushroom). Microbiology, 144 ( Pt 8), 2345-53.