My group studies cytoskeletal organisation and trafficking processes, which contribute to cell polarisation, differentiation, vertebrate development and human disease. We are interested in understanding, at a systems level, in how different aspects of cell architecture are coordinated.
The main focus of our studies is the microtubule cytoskeleton. Our research relies on combining high-resolution live cell imaging and quantitative analysis of cytoskeletal dynamics, measurement of protein dynamics using advanced microscopic assays, in vitro reconstitution of dynamic cytoskeleton-based processes and different methods of identification of protein-protein interactions, such as mass spectrometry. In addition to cultured cells, we employ mouse knockout technologies and couple genetic modification (such as GFP knock-in technology) to live cell and tissue imaging. In collaboration with mathematicians we are working on development of automated analysis and modelling of cytoskeletal dynamics and vesicle transport.
Microtubule-based processes are essential for cell division, normal functioning of large cells such as neurons and for intracellular transport of pathogens; therefore, our studies are relevant for combating abnormal cell proliferation, neurodegeneration and infectious diseases.