Publications
2024
Scholarly publications
Akdag, M., van Schijndel, V., & Sinnige, T. (2024). Islet amyloid polypeptide tagged with green fluorescent protein localises to mitochondria and forms filamentous aggregates in Caenorhabditis elegans. Biophysical Chemistry, 307, Article 107180. [DOI] [Repository]
2023
Scholarly publications
Elenbaas, B. O. W., Kremsreiter, S. M., Khemtemourian, L., Killian, J. A., & Sinnige, T. (2023). Fibril elongation by human islet amyloid polypeptide is the main event linking aggregation to membrane damage. BBA Advances, 3, 1-9. Article 100083. [DOI] [Repository]
2022
Scholarly publications
Elenbaas, B. O. W., Khemtemourian, L., Killian, J. A., & Sinnige, T. (2022). Membrane-Catalyzed Aggregation of Islet Amyloid Polypeptide Is Dominated by Secondary Nucleation. Biochemistry, 61(14), 1465-1472. [DOI] [Repository]
Sinnige, T. (2022). Molecular mechanisms of amyloid formation in living systems. Chemical Science, 13(24), 7080-7097. [DOI] [Repository]
2021
Scholarly publications
Molenkamp, J., den Outer, A., van Schijndel, V., & Sinnige, T. (2021). Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans. Journal of visualized experiments : JoVE, 178, 1-13. Article e63365. [DOI] [Repository]
Hardenberg, M. C., Sinnige, T., Casford, S., Dada, S. T., Poudel, C., Robinson, E. A., Fuxreiter, M., Kaminski, C. F., Kaminski Schierle, G. S., Nollen, E. A. A., Dobson, C. M., & Vendruscolo, M. (2021). Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assemblies. Journal of Molecular Cell Biology, 13(4), 282-294. [DOI]
Perni, M., van der Goot, A., Limbocker, R., van Ham, T. J., Aprile, F. A., Xu, C. K., Flagmeier, P., Thijssen, K., Sormanni, P., Fusco, G., Chen, S. W., Challa, P. K., Kirkegaard, J. B., Laine, R. F., Ma, K. Y., Müller, M. B. D., Sinnige, T., Kumita, J. R., Cohen, S. I. A., ... Dobson, C. M. (2021). Comparative Studies in the A30P and A53T α-Synuclein C. elegans Strains to Investigate the Molecular Origins of Parkinson's Disease. Frontiers in Cell and Developmental Biology, 9, 1-10. Article 552549. [DOI] [Repository]
Sinnige, T., Meisl, G., Michaels, T. C. T., Vendruscolo, M., Knowles, T. P. J., & Morimoto, R. I. (2021). Kinetic analysis reveals that independent nucleation events determine the progression of polyglutamine aggregation in C. elegans. Proceedings of the National Academy of Sciences of the United States of America, 118(11), 1-7. Article e2021888118. [DOI] [Repository]
2020
Scholarly publications
Sinnige, T., Yu, A., & Morimoto, R. I. (2020). Challenging Proteostasis: Role of the Chaperone Network to Control Aggregation-Prone Proteins in Human Disease. In M. L. Mendillo, D. Pincus, & R. Scherz-Shouval (Eds.), HSF1 and Molecular Chaperones in Biology and Cancer (1 ed., pp. 53-68). (Advances in Experimental Medicine and Biology; Vol. 1243). Springer. [DOI]
Sinnige, T., Stroobants, K., Dobson, C. M., & Vendruscolo, M. (2020). Biophysical studies of protein misfolding and aggregation in in vivo models of Alzheimer's and Parkinson's diseases. Quarterly Reviews of Biophysics, 53, 1-43. Article e10. [DOI] [Repository]
2019
Scholarly publications
Sinnige, T., Ciryam, P., Casford, S., Dobson, C. M., De Bono, M., & Vendruscolo, M. (2019). Expression of the amyloid-β peptide in a single pair of C. elegans sensory neurons modulates the associated behavioural response. PLoS One, 14(5), Article e0217746. [DOI]
Huang, C., Wagner-Valladolid, S., Stephens, A. D., Jung, R., Poudel, C., Sinnige, T., Lechler, M. C., Schörit, N., Lu, M., Laine, R. F., Michel, C. H., Vendruscolo, M., Kaminski, C. F., Schierle, G. S. K., & David, D. C. (2019). Intrinsically aggregation-prone proteins form amyloid-like aggregates and contribute to tissue aging in caenorhabditis elegans. eLife, 8, Article e43059. [DOI]
Laine, R. F., Sinnige, T., Ma, K. Y., Haack, A. J., Poudel, C., Gaida, P., Curry, N., Perni, M., Nollen, E. A. A., Dobson, C. M., Vendruscolo, M., Kaminski Schierle, G. S., & Kaminski, C. F. (2019). Fast Fluorescence Lifetime Imaging Reveals the Aggregation Processes of α-Synuclein and Polyglutamine in Aging Caenorhabditis elegans. ACS Chemical Biology, 14(7), 1628-1636. [DOI]
2018
Scholarly publications
de Agrela Pinto, C., Mance, D., Sinnige, T., Daniëls, M., Weingarth, M., & Baldus, M. (2018). Formation of the β-barrel assembly machinery complex in lipid bilayers as seen by solid-state NMR. Nature Communications, 9(1), Article 4135. [DOI] [Repository]
Baker, L. A., Sinnige, T., Schellenberger, P., De Keyzer, J., Siebert, C. A., Driessen, A. J. M., Baldus, M., & Grünewald, K. (2018). Combined 1 H-Detected Solid-State NMR Spectroscopy and Electron Cryotomography to Study Membrane Proteins across Resolutions in Native Environments. Structure, 26(1), 161-170.e3. [DOI] [Repository]
2015
Scholarly publications
Mance, D., Sinnige, T., Kaplan, M., Narasimhan, S., Daniëls, M., Houben, K., Baldus, M., & Weingarth, M. (2015). An Efficient Labelling Approach to Harness Backbone and Side-Chain Protons in 1H-Detected Solid-State NMR Spectroscopy. Angewandte Chemie - International Edition, 54(52), 15799-15803. [DOI] [Repository]
Sinnige, T., Weingarth, M., Daniels, M., Boelens, R., Bonvin, A. M. J. J., Houben, K., & Baldus, M. (2015). Conformational Plasticity of the POTRA 5 Domain in the Outer Membrane Protein Assembly Factor BamA. Structure, 23(7), 1317-1324. [DOI] [Repository]
Baker, L. A., Folkers, G. E., Sinnige, T., Houben, K., Kaplan, M., van der Cruijsen, E. A. W., & Baldus, M. (2015). Magic-Angle-Spinning Solid-State NMR of Membrane Proteins. Methods in Enzymology, 557, 307-328. [DOI] [Repository]
Sinnige, T., Houben, K., Pritisanac, I., Renault, M., Boelens, R., & Baldus, M. (2015). Insight into the conformational stability of membrane-embedded BamA using a combined solution and solid-state NMR approach. Journal of Biomolecular NMR, 61(3-4), 321-32. [DOI] [Repository]
2014
Scholarly publications
Sinnige, T., Weingarth, M., Renault, M., Baker, L., Tommassen, J., & Baldus, M. (2014). Solid-State NMR studies of full-length BamA in lipid bilayers suggest limited overall POTRA mobility. Journal of Molecular Biology, 426(9), 2009-2021. [DOI]
Sinnige, T., Daniëls, M., Baldus, M., & Weingarth, M. (2014). Proton clouds to measure long-range contacts between nonexchangeable side chain protons in solid-state NMR. Journal of the American Chemical Society, 136(12), 4452-4455. [DOI]
Sinnige, T. (2014). Plasticity of the β-barrel assembly machinery investigated by NMR. [Doctoral thesis 1 (Research UU / Graduation UU), Utrecht University]. Utrecht University. [Repository]
Other output
Sinnige, T., Houben, K., Weingarth, M., Baker, L., Boelens, R., & Baldus, M. (2014). Protein Plasticity and Protein-Lipid Interactions of the Beta-Barrel Assembly Machinery. Biophysical Journal, 106(2), 47A-47A. [DOI] [Repository]
2011
Scholarly publications
Karagoz, E., dos santos Duarte, A., Ippel, H., Uetrecht, C., Sinnige, T., Van Rosmalen, M., Hausmann, J., Heck, A. J. R., Boelens, R., & Rüdiger, S. G. D. (2011). N-terminal domain of human Hsp90 triggers binding to the cochaperone p23. Proceedings of the National Academy of Sciences of the United States of America, 108(2), 580-585. [DOI]
Karagöz, G. E., Sinnige, T., Hsieh, O., & Rüdiger, S. G. D. (2011). Expressed protein ligation for a large dimeric protein. Protein Engineering, Design & Selection, 24(6), 495-501. [DOI] [Repository]
2010
Scholarly publications
Sinnige, T., Karagöz, G. E., & Rüdiger, S. G. D. (2010). Protein folding and chaperones. In Encyclopedia of life sciences Wiley.