Rolf Sparidans was educated in Analytical Chemistry (1990) at the Eindhoven Technical University, Fac. Chemical Engineering, The Netherlands. He was further specialized in chromatographic bioanalysis as PhD (1997) at the Leiden University Medical Center, dept. Clinical Pharmacy, and as a post-doc (1997-2001) at the Utrecht University, dept. of Biomedical Analysis. Nowadays he is working as assistant professor at the Utrecht University, dept. of Pharmaceutical Sciences on bioanalysis using liquid chromatography-mass spectrometry. Research activities are focused on bioanalytical methods for quantification of drugs and especially of relevant endogenous metabolomic compounds in pharmacological (kidney, lung, intestine, and brain) research models. For example: protein-bound uremic toxins (PBUTs) and short chain fatty acids (SCFAs). In addition to teaching in analytical courses he is also well experienced in teaching basic pharmacokinetics and biotransformation.