Roos Masereeuw obtained her M.Sc. in Biopharmaceutical Sciences from Leiden University in 1991, and a Ph.D. from Radboud University in 1997. In 1997, she joined the Radboudumc as Assistant Professor and in 2002 as Associate Professor after obtaining a prestigious Aspasia award from The Netherlands Organisation for Scientific Research (NWO).
In 1995, Masereeuw worked in the lab of Dr. D.S. Miller (Lab. Pharmacology and Chemistry, NIEHS/NIH, North Carolina, USA), and visited Mount Desert Island Biological Laboratory (Maine, USA) as principal investigator in 1997, 1998, 2010 and 2014.
Since 2014, Roos Masereeuw has been appointed as a professor at the Department of Pharmaceutical Sciences at Utrecht University, and was Scientific Director of the Utrecht Institute of Pharmaceutical Sciences (UIPS) from November 2018 to February 2023. Since February 2023, she combines her position as full professor with the position of Vice-Dean of Research at the Faculty of Science of Utrecht University.
 

Masereeuw is author of over 270 publications, book chapters and review articles, and has presented numerous lectures and workshops, including at the annual meetings of the International Union of Basic and Clinical Pharmacology (IUPHAR), the American Association of Pharmaceutical Sciences (AAPS), the International Society for the Study of Xenobiotics (ISSX) and British Pharmacological Society.
 

In 2009, she received the Dutch Pharmacological Society (NVF) Schering-Plough Pharmacology Award and in 2010 the Galenus Research prize as recognitions of her contributions to pharmacological research. In 2015 she was elected fellow of the American Association of Pharmaceutical Sciences. In 2018 she was elected Supervisor of the year at the Graduate School of Life Sciences, Utrecht University (15 PhD programs, approx. 1,800 PhD candidates). In 2022, she was elected as member of The Royal Holland Society of Sciences and Humanities (KHMW).