Plant cells are enclosed by a carbohydrate-rich cell wall that provides structural integrity and mediates interactions with the environment. How mechanical signals are sensed and integrated during growth remains largely unknown. Loss of the cell wall-associated receptor FERONIA (FER) causes hypersensitivity to mechanical stimulation. To investigate this mechanism, we will combine high-resolution live root imaging with microspheres-based mechanical stimulation followed by (phospho)proteomic profiling. In parallel, we will screen for and genetically characterize suppressors of the hypersensitive response of fer mutants under mechanical stress. These approaches will help define FERONIA’s upstream and downstream regulators and uncover key mechanosensory pathways.