Publications
2023
Scholarly publications
Umair, M., Scheeren, V. F. D. C., Beitsma, M. M., Colleoni, S., Galli, C., Lazzari, G., de Ruijter-Villani, M., Stout, T. A. E., & Claes, A. (2023). In Vitro-Produced Equine Blastocysts Exhibit Greater Dispersal and Intermingling of Inner Cell Mass Cells than In Vivo Embryos. International Journal of Molecular Sciences, 24(11), [9619]. https://doi.org/10.3390/ijms24119619
Umair, M., Beitsma, M., de Ruijter-Villani, M., Deelen, C., Herrera, C., Stout, T. A. E., & Claes, A. (2023). Vitrifying expanded equine embryos collapsed by blastocoel aspiration is less damaging than slow-freezing. Theriogenology, 202, 28-35. https://doi.org/10.1016/j.theriogenology.2023.02.028
Wu, J., Larreategui-Aparicio, A., Lambers, M. L. A., Bodor, D. L., Klaasen, S. J., Tollenaar, E., de Ruijter-Villani, M., & Kops, G. J. P. L. (2023). Microtubule nucleation from the fibrous corona by LIC1-pericentrin promotes chromosome congression. Current Biology, 33(5), 912-925.e6. https://doi.org/10.1016/j.cub.2023.01.010
2022
Scholarly publications
Gibson, C., de Ruijter-Villani, M., & Stout, T. A. E. (2022). Insulin-like growth factor system components expressed at the conceptus-maternal interface during the establishment of equine pregnancy. Frontiers in Veterinary Science, 9, 1-15. [912721]. https://doi.org/10.3389/fvets.2022.912721
De Coster, T., Masset, H., Tšuiko, O., Catteeuw, M., Zhao, Y., Dierckxsens, N., Aparicio, A. L., Dimitriadou, E., Debrock, S., Peeraer, K., de Ruijter-Villani, M., Smits, K., Van Soom, A., & Vermeesch, J. R. (2022). Parental genomes segregate into distinct blastomeres during multipolar zygotic divisions leading to mixoploid and chimeric blastocysts. Genome Biology, 23(1), 1-29. [201]. https://doi.org/10.1186/s13059-022-02763-2
2021
Scholarly publications
Schneider, I., de Ruijter-Villani, M., Hossain, M. J., Stout, T. A. E., & Ellenberg, J. (2021). Dual spindles assemble in bovine zygotes despite the presence of paternal centrosomes. Journal of Cell Biology, 220(11), 1-24. [e202010106]. https://doi.org/10.1083/jcb.202010106
D' Fonseca, N. M. M., Gibson, C. M. E., van Doorn, D. A., Roelfsema, E., de Ruijter-Villani, M., & Stout, T. A. E. (2021). Effect of Overfeeding Shetland Pony Mares on Embryonic Glucose and Lipid Accumulation, and Expression of Imprinted Genes. Animals, 11(9), 1-23. [2504]. https://doi.org/10.3390/ani11092504
D' Fonseca, N. M. M., Beukers, M., Wijnberg, I. D., Navas de Solis, C., de Ruijter-Villani, M., van Doorn, D. A., Stout, T. A. E., & Roelfsema, E. (2021). Effect of a long-term high-energy diet on cardiovascular parameters in Shetland pony mares. Journal of Veterinary Internal Medicine, 35(5), 2427-2436. https://doi.org/10.1111/jvim.16229
Rizzo, M., Stout, T. A. E., Cristarella, S., Quartuccio, M., Kops, G. J. P. L., & De Ruijter-Villani, M. (2021). Compromised MPS1 Activity Induces Multipolar Spindle Formation in Oocytes From Aged Mares: Establishing the Horse as a Natural Animal Model to Study Age-Induced Oocyte Meiotic Spindle Instability. Frontiers in Cell and Developmental Biology, 9, 1-10. [657366]. https://doi.org/10.3389/fcell.2021.657366
D’fonseca, N. M. M., Gibson, C. M. E., Hummel, I., van Doorn, D. A., Roelfsema, E., Stout, T. A. E., van den Broek, J., & de Ruijter-Villani, M. (2021). Overfeeding extends the period of annual cyclicity but increases the risk of early embryonic death in shetland pony mares. Animals, 11(2), 1-12. [361]. https://doi.org/10.3390/ani11020361
2020
Scholarly publications
Rizzo, M., du Preez, N., Ducheyne, K. D., Deelen, C., Beitsma, M. M., Stout, T. A. E., & de Ruijter-Villani, M. (2020). The horse as a natural model to study reproductive aging-induced aneuploidy and weakened centromeric cohesion in oocytes. Aging, 12(21), 22220-22232. https://doi.org/10.18632/aging.104159
Rizzo, M., Deelen, C., Beitsma, M., Stout, T. A., & de Ruijter-Villani, M. (2020). In vitro matured horse oocytes from aged mares show weakened centromeric cohesion and a higher incidence of aneuploidy. Journal of Equine Veterinary Science, 89, [103028]. https://doi.org/10.1016/j.jevs.2020.103028
d' Fonseca, N. M. M., Gibson, C. M. E., van Doorn, D. A., de Ruijter-Villani, M., Stout, T. A. E., & Roelfsema, E. (2020). Effect of long-term overfeeding of a high-energy diet on glucose tolerance in Shetland pony mares. Journal of Veterinary Internal Medicine, 34(3), 1339-1349. https://doi.org/10.1111/jvim.15788
Gibson, C., de Ruijter-Villani, M., Bauersachs, S., & Stout, T. A. E. (2020). Asynchronous Embryo Transfer Followed by Comparative Transcriptomic Analysis of Conceptus Membranes and Endometrium Identifies Processes Important to the Establishment of Equine Pregnancy. International Journal of Molecular Sciences, 21(7), [2562]. https://doi.org/10.3390/ijms21072562
2019
Scholarly publications
Ducheyne, K. D., Rizzo, M., Cuervo-Arango, J., Claes, A., Daels, P. F., Stout, T. A. E., & de Ruijter-Villani, M. (2019). Invitro production of horse embryos predisposes to micronucleus formation, whereas time to blastocyst formation affects likelihood of pregnancy. Reproduction, Fertility and Development, 31(12), 1830-1839. https://doi.org/10.1071/RD19227
Silva, E. S. M., Cuervo-Arango, J., de Ruijter-Villani, M., Klose, K., Oquendo, P. S., & Stout, T. A. E. (2019). Effect of the duration of estradiol priming prior to progesterone administration on endometrial gene expression in anestrous mares. Theriogenology, 131, 96-105. https://doi.org/10.1016/j.theriogenology.2019.03.025
Ducheyne, K. D., Rizzo, M., Daels, P. F., Stout, T. A. E., & de Ruijter-Villani, M. (2019). Vitrifying immature equine oocytes impairs their ability to correctly align the chromosomes on the MII spindle. Reproduction, Fertility and Development, 31(8), 1330-1338. https://doi.org/10.1071/RD18276
Rizzo, M., Ducheyne, K. D., Deelen, C., Beitsma, M., Cristarella, S., Quartuccio, M., Stout, T. A. E., & de Ruijter-Villani, M. (2019). Advanced mare age impairs the ability of in vitro matured oocytes to correctly align chromosomes on the metaphase plate. Equine Veterinary Journal, 252-257. https://doi.org/10.1111/evj.12995
2018
Scholarly publications
Rizzo, M., Kops, G. J. P. L., Deelen, C., Beitsma, M., Cristarella, S., Stout, T. A. E., & de Ruijter-Villani, M. (2018). Compromised Spindle Assembly Check-point Function in Oocytes From Aged Mares Impairs Correct Chromosome Alignment. Journal of Equine Veterinary Science, 66, 177. https://doi.org/10.1016/j.jevs.2018.05.069
Lawson, E. F., Gibb, Z., de Ruijter-Villani, M., Smith, N. D., Stout, T. A., Clutton-Brock, A., Aitken, J. R., & Swegen, A. (2018). Proteomic Analysis of Pregnant Mare Uterine Fluid. Journal of Equine Veterinary Science, 66, 171-172. https://doi.org/10.1016/j.jevs.2018.05.064
Gibson, C., de Ruijter-Villani, M., Rietveld, J., & Stout, T. A. E. (2018). Expression of glucose transporters in the endometrium and early conceptus membranes of the horse. Placenta, 68, 23-32. https://doi.org/10.1016/j.placenta.2018.06.308
Gibson, C., de Ruijter-Villani, M., Rietveld, J., & Stout, T. A. E. (2018). Amino acid transporter expression in the endometrium and conceptus membranes during early equine pregnancy. Reproduction, Fertility and Development, 30(12), 1675-1688. https://doi.org/10.1071/RD17352
Siegers, E. W., Villani, M., van Doorn, D. A., Stout, T. A. E., & Roelfsema, E. (2018). Ultrasonographic measurements of localized fat accumulation in Shetland pony mares fed a normal v. a high energy diet for 2 years. Animal, 12(8), 1602-1610. https://doi.org/10.1017/S1751731117003251
Cuervo-Arango, J., Claes, A. N., Ruijter-Villani, M., & Stout, T. A. (2018). Likelihood of pregnancy after embryo transfer is reduced in recipient mares with a short preceding oestrus. Equine Veterinary Journal, 50(3), 386-390. https://doi.org/10.1111/evj.12739
2017
Scholarly publications
Gibson, C., de Ruijter-Villani, M., & Stout, T. A. E. (2017). Negative uterine asynchrony retards early equine conceptus development and upregulation of placental imprinted genes. Placenta, 57, 175-182. https://doi.org/10.1016/j.placenta.2017.07.007
Swegen, A., Grupen, C. G., Gibb, Z., Baker, M., de Ruijter - Villani, M., Smith, N. D., Stout, T., & Aitken, R. J. (2017). From Peptide Masses to Pregnancy Maintenance: A Comprehensive Proteomic Analysis of The Early Equine Embryo Secretome, Blastocoel Fluid and Capsule. Proteomics, 17(17-18), [1600433]. https://doi.org/10.1002/pmic.201600433
2016
Scholarly publications
Brogan, P. T., Henning, H., Stout, T. A. E., & de Ruijter-Villani, M. (2016). Relationship between colour flow Doppler sonographic assessment of corpus luteum activity and progesterone concentrations in mares after embryo transfer. Animal Reproduction Science, 166(March), 22-27. https://doi.org/10.1016/j.anireprosci.2015.12.010
De Ruijter-Villani, M., Deelen, C., & Stout, T. A. E. (2016). Expression of leukaemia inhibitory factor at the conceptus-maternal interface during preimplantation development and in the endometrium during the oestrous cycle in the mare. Reproduction, Fertility and Development, 28(10), 1642-1651. https://doi.org/10.1071/RD14334
2015
Scholarly publications
Siegers, E. W., Villani, M., & Roelfsema, E. (2015). Ultrasonographic measurements of localized fat accumulation in normal and high energy fed Shetland pony mares over time. Abstract from ECEIM congress 2015, Utrecht, Netherlands.
de Ruijter-Villani, M., van Tol, H. T. A., & Stout, T. A. E. (2015). Effect of pregnancy on endometrial expression of luteolytic pathway components in the mare. Reproduction, Fertility and Development, 27(5), 834-845. https://doi.org/10.1071/RD13381
de Ruijter-Villani, M., & Stout, T. A. E. (2015). The Role of Conceptus-maternal Signalling in the Acquisition of Uterine Receptivity to Implantation in Mammals. Reproduction in Domestic Animals, 50 (Suppl 3), 7-14. https://doi.org/10.1111/rda.12527
Other output
Wijnberg, I. D., Roelfsema, E., & Villani, M. (2015). Residual effects of high energy diet on blood pressure in obese Shetland ponies.. 124. Abstract from ECEIM congress 2015, Utrecht, Netherlands.
kerbert, P. M. P., Sonneveld, D., voskamp, J. P., de Graaf - Roelfsema, E., de Ruijter - Villani, M., & Back, W. (2015). Does an increased body weight influence the sagittal and carpa l range of motion in Shetland ponies at walk and trot?. Poster session presented at Voorjaarsdagen 2015, Amsterdam, Netherlands.
2014
Scholarly publications
Jenner, F., Närväinen, J., de Ruijter-Villani, M., Stout, T. A. E., van Weeren, P. R., & Brama, P. (2014). Magnetic resonance microscopy atlas of equine embryonic development. Equine Veterinary Journal, 46(2), 210-215. https://doi.org/10.1111/evj.12102
2013
Scholarly publications
Journée, S. L., Villani, M., Hendriks, W. K., & Stout, T. A. E. (2013). Efficacy of transvaginal ultrasound-guided twin reduction in the mare by embryonic or fetal stabbing compared with yolk sac or allantoic fluid aspiration. Theriogenology, 80(4), 346-349. https://doi.org/10.1016/j.theriogenology.2013.04.021
Villani, M., Boxtel, P. R., & Stout, T. A. E. (2013). Fibroblast growth factor-2 expression in the preimplantation equine conceptus and endometrium of pregnant and cyclic mares. Theriogenology, 80(9), 979-989. [9]. https://doi.org/10.1016/j.theriogenology.2013.07.024
2012
Scholarly publications
Panzani, S., Comin, A., Galeati, G., Romano, G., Villani, M., Faustini, M., & Veronesi, M. C. (2012). How type of parturition and health status influence hormonal and metabolic profiles in newborn foals. Theriogenology, 77(6), 1167-1177.
2011
Other output
Villani, M., & Stout, T. A. E. (Accepted/In press). Early pregnancy loss in the mare: is luteal failure the major threat?. Abstract from ESDAR Congress, Anatalaya (Turkey).
2010
Scholarly publications
Veronesi, M. C., Tosi, U., Villani, M., Govoni, N., Faustini, M., Kindahl, H., Madej, A., & Carluccio, A. (2010). Oxytocin, vasopressin, prostaglandin F2a, luteinizing hormone, testosterone, estrone sulfate and cortisol plasma concentrations after sexual stimulation in stallions. Theriogenology, 73, 460-467.
Veronesi, M. C., Villani, M., Wilsher, S., Contri, A., & Carluccio, A. (2010). A comparative stereological study of the term placenta in the donkey, pony and Thoroughbred. Theriogenology, 74, 627-631.
Other output
Villani, M., & Stout, T. A. E. (Accepted/In press). Effect of early pregnancy on the expression of genes associated with endometrial prostaglandin release.. 18-18. Abstract from International Workshop on Equine Reproductive Medicine.
Villani, M., Vriend, B. A. M., Paris, D. B. B. P., & Stout, T. A. E. (Accepted/In press). A role for leukaemia inhibitory factor (LIF) during implantation in the mare.. 309-309. Abstract from 10th International Symposium on Equine Reproduction.
Villani, M., & Stout, T. A. E. (Accepted/In press). The use of colour-flow Doppler ultrasonography in broodmare practice.. 250-253. Abstract from Fifth Leipziger Tierärztekongress.
Villani, M., & Stout, T. A. E. (Accepted/In press). Osteopontin: a role in equine implantation?. Abstract from Third Annual COST-Gemini meeting.
2009
Scholarly publications
Panzani, S., Villani, M., McGladdery, A., Magri, M., Galeati, G., Martino, P. A., & Veronesi, M. C. (2009). Concentrations of 15-ketodihydro-PGF2alfa, cortisol, and progesterone in the plasma of healthy and pathologic newborm foals. Theriogenology, 72, 1032-1040.
2008
Scholarly publications
Villani, M., & Romano, G. (2008). Induction of parturition with daily low-dose oxytocin injections in pregnant mares at term: clinical applications and limitations. Reproduction in Domestic Animals, 43, 481-483.
Other output
Villani, M., van Tol, H. T. A., & Stout, T. A. E. (Accepted/In press). Expression of mRNA for cyclooxygenase-2 (COX2), oxytocin receptors (OTR) and PGF2 receptors (PGFR) in the endometrium of cycling and early pregnant mares.. Abstract from ESDAR Annual Congress, Utrecht.
Journée, S. L., Villani, M., & Stout, T. A. E. (Accepted/In press). Equine twin reduction by fetal stabbing.. Abstract from Voorjaarsdagen, Amsterdam & Utrecht.