Dr. M.P. (Marc) Baggelaar

Assistant Professor
Biomol.Mass Spectrometry & Proteom.

Bioorthogonal Ligation and Activity-based Protein Profiling

Figure 1, Workflow for site-specific activity-based protein profiling using phosphonate enrichment handles 

We are developing new enrichment strategies for the selective enrichment of posttranslational modifications and chemically tagged proteins.

Current research focus:

  • IMAC-enrichable affinity tags (PhosID) that are compatible with ABPP studies.
  • Exact drug binding site identification using phosphonate handles 
  • Fragmentation behavior of ABPs to allow identification of their protein binding sites.

Figure 2, identification of specific ABP binding sites by MS/MS fragmentation