EDUCATION/TRAINING:
2012-2018: PhD in Experimental Hepatology & Biochemistry, Tytgat Institute for Liver and Intestinal Research, Amsterdam UMC, University of Amsterdam
2015: Visiting Scientist at Weill Cornell Medical College, New York City, U.S.
2010-2012: Internal Medicine Residency, 2010-2012, National Taiwan University Hospital, Taipei, Taiwan
2004-2010: MD degree, June 2010, National Taiwan University, Taipei, Taiwan
Positions & Employment
Oct 2024 – Present
Assistant professor, Division Cell Biology, Metabolism & Cancer, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University
Sep 2021 – Oct 2024
Postdoctoral fellow, Division Cell Biology, Metabolism & Cancer, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University
Jan 2022 – July 2023
Project leader, Tytgat Institute for Liver and Intestinal Research, Amsterdam UMC, Amsterdam. Supported by ZonMw Off-Road Grant 2020: “A cholestatic itch mouse model with state-of-the-art scratch monitoring for the identification of pruritogens in cholestatic liver diseases” (Personal grant)
Sep 2020 – Sep 2021
Manager of Seahorse Facility, Amsterdam UMC, Location AMC
Sep 2020 – Sep 2021
Postdoctoral fellow, Tytgat Institute for Liver and Intestinal Research, Amsterdam UMC, Amsterdam, the Netherlands. Supported by Talent Development Grant of Research Institute Amsterdam Gastroenterology, Endocrinology & Metabolism (Personal grant).
Sep 2018 – Sep 2020
Postdoctoral fellow, Tytgat Institute for Liver and Intestinal Research, Amsterdam UMC, Amsterdam, the Netherlands. Supported by KWF Unique High-Risk Project #11652 (Team member).
May 2012 – Sep 2018
PhD program, Tytgat Institute for Liver and Intestinal Research, Amsterdam UMC, Amsterdam, the Netherlands
Aug 2010 – Feb 2012
Medical Doctor, Internal Medicine Resident, National Taiwan University Hospital, Taipei, Taiwan
Grants and Awards
1. NWO-ENW Veni, €280,000, 2022
“NASH-in-a-dish: Identifying targetable metabolite crosstalk for NASH treatment”
ZonMw Off-Road grant, €100,000, 2021
“A cholestatic itch mouse model with state-of-the-art scratch monitoring for the identification of pruritogens in cholestatic liver diseases”
AG&M talent development grant, €75,000, 2019
“Metabolic regulation of macrophage polarization by the emerging bioenergetic regulator soluble adenylyl cyclase”
Young investigator bursary, EASL Monothematic Conference “Cholangiocytes in Health and Disease”, 2017
“NADH/NAD+ redox status represents an abridged metabolic index that regulates intrinsic apoptosis in human cholangiocytes”
AMC Young Talent Fund Travel Grant, €5000, 2015
“Effect of bile salts on soluble adenylyl cyclase activity in human cholangiocyte cell line H69”
First Prize for teamwork project Scientific-BREAKOUT, Dutch Liver Retreat, 2016
“Development of an anti-viral peptide from NTCP fragments to block HBV entry”
First Prize Speed-date project, Dutch Liver Retreat, 2014
“Role of soluble adenylyl cyclase in activation of hepatic stellate cells”
Scientific Output
Pre-prints
Marsee, A., Ritchie, A., Myszczyszyn, A., Ye S., Chang, J.-C., Ardisasmita, A.I., Joore, I.P., Castro-Alpízar, J., Fuchs, S.A., Schneeberger, K., Spee, B. (2024) Mass Generation and Long-term Expansion of Hepatobiliary Organoids from Adult Primary Human Hepatocytes. bioRxiv https://doi.org/10.1101/2024.06.10.598262
Original research articles
Bizerra, P.F.V., Gilglioni, E.H., Li, H.J., Go, S., Oude Elferink, R.P.J., Verhoeven, A.J., Chang, J.-C.# (2024) Opposite regulation of glycogen metabolism by cAMP produced in the cytosol and at the plasma membrane. Biochimica et Biophysica Acta — Mol Cell Research. 1871(1):119585
S. Go, Li, H.L., Chang, J.-C., Verhoeven, A.J., Oude Elferink, R.P.J. (2023) Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles. Traffic. 24(9):413-430.
Montoya, M.M., Van Slobbe, G.J.J., Chang, J.-C., Zaal, E.A., Berkers, C.R. (2023) Metabolic changes underlying drug resistance in the multiple myeloma tumor microenvironment. Frontiers in Oncology. 6:13:1155621.
Herta, T.*, Kersten, R.*, Chang, J.-C., Hubers, L., Go, S., Tolenaars, D., Paulusma, C.C., Nathanson, M.H., Oude Elferink, R.P.J., Van de Graaf, S.F.J., Beuers, U. (2022) Role of the IgG4-related cholangitis autoantigen annexin A11 in cholangiocyte protection. Hepatology. 76(2):319-331.
Van Dijk, A., Anten, J., Bakker, A., Evers, N., Hoekstra, A.T., Chang, J.-C., Scheenstra, M.R., Veldhuizen, E.J.A., Netea, M.G., Berkers, C.R., Haagsman, H.P. (2022) Innate Immune Training of Human Macrophages by Cathelicidin Analogs. Frontiers in Immunology. 26:13:777530.
Go, S., Kramer, T.K., Verhoeven, A.J., Oude Elferink, R.P.J., Chang, J.-C.# (2021) The extracellular lactate-to-pyruvate ratio modulates the sensitivity for oxidative stress-induced apoptosis via the cytosolic NADH/NAD+ redox state. Apoptosis. 26:38-51
Chang, J.-C.#, Go, S., Gilglioni E.H., Duijst, S., Panneman, D.M., Rodenburg, R.J., Li, H.L., Huang, H.-L., Levin, L.R., Buck J., Verhoeven, A.J., Oude Elferink, R.P.J. (2021) Soluble adenylyl cyclase regulates the cytosolic NADH/NAD+ redox state and the bioenergetic switch between glycolysis and oxidative phosphorylation. Biochimica et Biophysica Acta — Bioenergetics. 1862(4):148367
Van der Mark, V.A., Adam, A.A.A., Chang, J.-C., Oude Elferink, R.P.J., Chamuleau, R.A.F.M., Hoekstra, R. (2020) Overexpression of the constitutive androstane receptor and shaken 3D-culturing increase biotransformation and oxidative phosphorylation and sensitivity to mitochondrial amiodarone toxicity of HepaRG cells. Toxicology and Applied Pharmacology. 399:115055.
Gilglioni, E.H.*, Chang, J.C.*, Duijst S., Go, S., Adam, A.A.A., Hoekstra, R., Verhoeven, A.J., Ishii-Iwamoto, E.L., Oude Elferink, R.P.J. (2018). Improved oxygenation dramatically alters metabolism and gene expression in cultured primary mouse hepatocytes. Hepatology Communications. 2(3):299-312.
Van Wenum, M., Adam, A.A.A., Van der Mark, V.A., Chang, J.C., Wildenberg, M.E., Hendriks E.J., Jongejan A., Moerland, P.D., Van Gulik, T.M., Oude Elferink, R.P.J., Chamuleau, R.A.F.M., Hoekstra, R. (2018). Oxygen drives hepatocyte differentiation and phenotype stability in liver cell lines. Journal of Cell Communication and Signaling. 12(3):575-588.
Van der Mark, V.A., De Jonge H.R., Chang, J.C., Ho-Mok K.S., Duijst S., Vidović, D., Carlon, M.S., Oude Elferink, R.P.J., Paulusma C.C. (2016) The phospholipid flippase ATP8B1 mediates apical localization of the cystic fibrosis transmembrane regulator. Biochimica et Biophysica Acta. 1863(9):2280-2288.
Chang, J.C., Go, S., De Waart, D.R., Munoz-Garrido, P., Beuers, U., Paulusma, C.C., Oude Elferink, R.P.J. (2016). Soluble adenylyl cyclase regulates bile salt-Induced apoptosis in human cholangiocytes. Hepatology. 64(2):522-534.
Zweers, S.J., Shiryaev, A., Komuta, M., Vesterhus, M., Hov, J.R., Perugorria, M.J., De Waart, D.R., Chang, J.C., Tol, S., Te Velde, A.A., De Jonge, W.J., Banales, J.M., Roskams, T., Beuers, U., Karlsen, T.H., Jansen, P.L., Schaap, F.G. (2016). Elevated interleukin-8 in bile of patients with primary sclerosing cholangitis. Liver International. 36(9):1370-1377.
Mardones, P.*, Chang, J.C.*, Oude Elferink, R.P.J. (2014). Cyclic AMP and alkaline pH downregulate carbonic anhydrase 2 in mouse fibroblasts. Biochimica et Biophysica Acta. 1840(6):1765-1770.
Review articles
Verheijen, F.W.M., Tran T.N.M., Chang, J.-C., Broere, F., Zaal, E.A., Berkers, C.R. (2024) Deciphering metabolic crosstalk in context: lessons from inflammatory diseases. Molecular Oncology. 2024 Jan 26. doi: 10.1002/1878-0261.13588.
Li, H.L., Go, S., Chang, J.-C., Verhoeven, A.J., Oude Elferink, R.P.J. (2024) Soluble adenylyl cyclase, the cell-autonomous member of the family. Biochimica et Biophysica Acta — Molecular Basis of Disease. 1870(2):166936.
Chang, J.C., Go, S., Verhoeven, A.J., Beuers, U., Oude Elferink, R.P.J. (2018). Role of the bicarbonate-responsive soluble adenylyl cyclase in cholangiocyte apoptosis in primary biliary cholangitis; a new hypothesis. Biochimica et Biophysica Acta. 1864(4 Pt B):1232-1239.
Chang, J.C., Beuers U., Oude Elferink, R.P.J. (2017). The emerging role of soluble adenylyl cyclase in primary biliary cholangitis. Digestive Diseases. 35(3):217-223.
Chang, J.C., Oude Elferink, R.P.J. (2014). Role of the bicarbonate-responsive soluble adenylyl cyclase in pH sensing and metabolic regulation. Frontiers in Physiology. 10;5:42
Magazine interview:
“Coral’s pH Sensor Identified” The Scientist, Nov 1, 2017 by Ashley P. Taylor
(https://www.the-scientist.com/daily-news/corals-ph-sensor-identified-30666)