dr. H.L.J. (Hans) Wienk
Gegenereerd op 2018-09-22 19:02:09


Currently I am the Project Manager for the structural biology project iNEXT, stimulating researchers from academia and industry to obtain access, expert supervision and training collectively offered by more than twenty European large structural biology laboratories. Here I oversee this European Union-funded project's progress, organize meetings, and oversee all networking and access activities. Other responsibilities are the project’s website, its financial and legal aspects, and the preparation of reports and publications.

In parallel, as Facility Manager I oversee the performance of the solution state NMR spectrometers in the Utrecht NMR group, ranging from 500-950 MHz. For several decades our NMR machines form a national facility (SONNMRLSF, uNMR-NL) as well as a European facility through different EU-funded initiatives (currently Instruct-ERIC, iNEXT). Besides dealing with the organization of this laboratory and reaching local research targets, I receive researchers from academia and industry, from The Netherlands and abroad. In many collaborative projects targeting mostly biochemical but often also analytical chemistry questions, I collect NMR data and am heavily involved in data interpretation, at the same time providing research training, often also in a supervisory role.

Most of the research I am currently involved in relates to the application of a wide range of solution state NMR techniques to diverse, mainly biologically relevant systems. Here, focus lies on the determination of structure, function and dynamics of proteins.

Strategic themes / Focus areas
Gegenereerd op 2018-09-22 19:02:09
Curriculum vitae

Hans Wienk (1970) studied Chemistry at Utrecht University, The Netherlands and obtained his PhD in the group Biochemistry of Membranes (Prof. Dr. B. de Kruijff) in 2000. Partly financed by an Alexander von Humboldt grant, he then stayed as a research fellow at the Goethe University in Frankfurt am Main, Germany (Biophysical Chemistry - Prof. Dr. H. Rueterjans). In 2002 he joined the NMR group at Utrecht University as a research fellow with Prof Dr. Rob Kaptein and Prof. Dr. Rolf Boelens. In 2008 he became facility manager in the same group where he is responsible for providing (inter)national guests with useful NMR data and analyses. In 2015 he became also project manager for the EU-H2020 structural biology initiative iNEXT.

Gegenereerd op 2018-09-22 19:02:09
All publications
  2018 - Scholarly publications
Corbeski, Ivan, Dolinar, Klemen, Wienk, Hans, Boelens, Rolf & van Ingen, Hugo (14.06.2018). DNA repair factor APLF acts as a H2A-H2B histone chaperone through binding its DNA interaction surface. Nucleic Acids Research
  2016 - Scholarly publications
Dongre, Ramachandra, Folkers, Gert E, Gualerzi, Claudio O, Boelens, Rolf & Wienk, Hans (2016). A model for the interaction of the G3-subdomain of Geobacillus stearothermophilus IF2 with the 30S ribosomal subunit. Protein Science, 25 (9), (pp. 1722-33) (12 p.). © 2016 The Protein Society..
Zhang, Ruiyan, Loers, Gabriele, Schachner, Melitta, Boelens, Rolf, Wienk, Hans, Siebert, Simone, Eckert, Thomas, Kraan, Stefan, Rojas-Macias, Miguel A., Lütteke, Thomas, Galuska, Sebastian P., Scheidig, Axel, Petridis, Athanasios K., Liang, Songping, Billeter, Martin, Schauer, Roland, Steinmeyer, Jürgen, Schröder, Jens Michael & Siebert, Hans Christian (06.05.2016). Molecular Basis of the Receptor Interactions of Polysialic Acid (polySia), polySia Mimetics, and Sulfated Polysaccharides. ChemMedChem, 11 (9), (pp. 990-1002) (13 p.).
Constant, Sandra, Wienk, Hans L J, Frissen, Augustinus E., Peinder, Peter De, Boelens, Rolf, Van Es, Daan S., Grisel, Ruud J H, Weckhuysen, Bert M., Huijgen, Wouter J J, Gosselink, Richard J A & Bruijnincx, Pieter C A (2016). New insights into the structure and composition of technical lignins - A comparative characterisation study. Green Chemistry, 18 (9), (pp. 2651-2665) (15 p.).
Oliveira, Luciana Coutinho, Souza, Diorge Paulo, Oka, Gabriel Umaji, Lima, Filipe da Silva, Oliveira, Ronaldo Junio, Favaro, Denize Cristina, Wienk, Hans, Boelens, Rolf, Farah, Chuck Shaker & Salinas, Roberto Kopke (04.10.2016). VirB7 and VirB9 Interactions Are Required for the Assembly and Antibacterial Activity of a Type IV Secretion System. Structure with Folding & design, 24 (10), (pp. 1707-1718). Copyright © 2016 Elsevier Ltd. All rights reserved..
  2015 - Scholarly publications
Furse, Samuel, Wienk, Hans, Boelens, Rolf, de Kroon, Anton I. P. M. & Killian, J. Antoinette (14.09.2015). E. coli MG1655 modulates its phospholipid composition through the cell cycle. FEBS Letters, 589 (19), (pp. 2726-2730) (5 p.).
Nieto, Lidia, Tharun, Inga M, Balk, Mark, Wienk, Hans, Boelens, Rolf, Ottmann, Christian, Milroy, Lech-Gustav & Brunsveld, Luc (20.11.2015). Estrogen Receptor Folding Modulates cSrc Kinase SH2 Interaction via a Helical Binding Mode. ACS Chemical Biology, 10 (11), (pp. 2624-32) (9 p.).
Moore, Eli K, Hopmans, Ellen C, Rijpstra, W Irene C, Sánchez-Andrea, Irene, Villanueva, Laura, Wienk, Hans, Schoutsen, Frans, Stams, Alfons J M & Sinninghe Damsté, Jaap S (2015). Lysine and novel hydroxylysine lipids in soil bacteria - amino acid membrane lipid response to temperature and pH in Pseudopedobacter saltans. Frontiers in Microbiology, 6.
Schwede, Frank, Bertinetti, Daniela, Langerijs, Carianne N, Hadders, Michael A, Wienk, Hans, Ellenbroek, Johanne H, de Koning, Eelco J P, Bos, Johannes L, Herberg, Friedrich W, Genieser, Hans-Gottfried, Janssen, Richard A J & Rehmann, Holger (2015). Structure-guided design of selective epac1 and epac2 agonists. PLoS Biology, 13 (1).
Faridounnia, Maryam, Wienk, Hans, Kovacic, Lidija, Folkers, Gert E., Jaspers, Nicolaas G. J., Kaptein, R, Hoeijmakers, Jan H. J. & Boelens, Rolf (14.08.2015). The Cerebro-oculo-facio-skeletal Syndrome Point Mutation F231L in the ERCC1 DNA Repair Protein Causes Dissociation of the ERCC1-XPF Complex. Journal of Biological Chemistry, 290 (33), (pp. 20541-20555) (15 p.).
  2014 - Scholarly publications
Scheepstra, Marcel, Nieto, Lidia, Hirsch, Anna K H, Fuchs, Sascha, Leysen, Seppe, Lam, Chan Vinh, In Het Panhuis, Leslie, Van Boeckel, Constant A A, Wienk, Hans, Boelens, Rolf, Ottmann, Christian, Milroy, Lech Gustav & Brunsveld, Luc (16.06.2014). A natural-product switch for a dynamic protein interface. Angewandte Chemie-International Edition, 53 (25), (pp. 6443-6448) (6 p.).
Ferguson, Fleur M., Dias, David M., Garcia Lopes Maia Rodrigues, João, Wienk, Hans, Boelens, Rolf, Bonvin, Alexandre M. J. J., Abell, Chris & Ciulli, Alessio (28.10.2014). Binding Hotspots of BAZ2B Bromodomain - Histone Interaction Revealed by Solution NMR Driven Docking. Biochemistry, 53 (42), (pp. 6706-6716) (11 p.).
Weiss, Jan, Wienk, Hans, Boelens, Rolf & Laschewsky, Andre (2014). Block Copolymer Micelles with an Intermediate Star-/Flower-Like Structure Studied by H-1 NMR Relaxometry. Macromolecular Chemistry and Physics, 215 (9), (pp. 915-919) (5 p.).
Jamalian, Azadeh, Sneekes, Evert-Jan, Wienk, Hans, Dekker, Lennard J. M., Ruttink, Paul J. A., Ursem, Mario, Luider, Theo M. & Burgers, Peter C. (2014). Identifying Ca2+-Binding Sites in Proteins by Liquid Chromatography-Mass Spectrometry Using Ca2+-Directed Dissociations. Molecular and Cellular Proteomics, 13 (11), (pp. 3177-3183) (7 p.).
Tuppo, Lisa, Spadaccini, Roberta, Alessandri, Claudia, Wienk, Hans, Boelens, Rolf, Giangrieco, Ivana, Tamburrini, Maurizio, Mari, Adriano, Picone, Delia & Ciardiello, Maria Antonietta (2014). Structure, Stability, and IgE Binding of the Peach Allergen Peamaclein (Pru p 7). Biopolymers, 102 (5), (pp. 416-425) (10 p.).
Popovic, Matija, Wienk, Hans, Coglievina, Maristella, Boelens, Rolf, Pongor, Sándor & Pintar, Alessandro (01.01.2014). The basic helix-loop-helix region of the transcriptional repressor hairy and enhancer of split 1 is preorganized to bind DNA. Proteins: Structure, Function and Genetics, 82 (4), (pp. 537-545) (9 p.).
  2013 - Scholarly publications
Augustyniak, W., Wienk, H.L.J., Boelens, R. & Reetz, M.T. (2013). 1H, 13C and 15N resonance assignments of wild-type Bacillus subtilis Lipase A and its mutant evolved towards thermostability. Biomolecular NMR Assignments, 7, (pp. 249-252) (4 p.).
Moore, E.K., Hopmans, E.C., Rijpstra, W.I.C., Villanueva, L., Dedysh, S.N., Kulichevskaya, I.S., Wienk, H.L.J., Schoutsen, F. & Sinninghe Damsté, J.S. (2013). Novel Mono-, Di-, and Trimethylornithine Membrane Lipids in Northern Wetland Planctomycetes. Applied and Environmental Microbiology, 79 (22), (pp. 6874-6884) (11 p.).
Wienk, H., Slootweg, J.C., Speerstra, S., Kaptein, R., Boelens, R. & Folkers, G.E. (2013). Solution structure of the helix hairpin helix domain of FAAP24 reveals that substrate specificity depends on an evolutionary conserved domain. Nucleic Acids Research, 41, (pp. 6739-6749) (11 p.).
Michalek, M., Sönnichsen, F.D., Wechselberger, R.W., Wienk, H.L.J., Leippe, M. & et al., [No Value] (2013). Structure and function of a unique pore-forming protein from a pathogenic acanthamoeba. Nature Chemical Biology, 9, (pp. 37-42) (6 p.).
  2012 - Scholarly publications
Augustyniak, W., Brzezinska, A.A., Pijning, Tjaard, Wienk, H.L.J., Boelens, R., Dijkstra, Bauke W. & Reetz, M.T. (01.04.2012). Biophysical characterization of mutants of Bacillus subtilis lipase evolved for thermostability: Factors contributing to increased activity retention. Protein Science, 21 (4), (pp. 487-497) (11 p.).
Stötzel, S., Schurink, M., Wienk, H.L.J., Siebler, U., Burg-Roderfeld, M., Eckert, T., Kulik, B., Wechselberger, R.W., Sewing, J., Steinmeyer, J., Oesser, S., Boelens, R. & Siebert, H.-C. (2012). Molecular Organization of Various Collagen Fragments as Revealed by Atomic Force Microscopy and Diffusion-Ordered NMR Spectroscopy. ChemPhysChem, 13 (13), (pp. 3117-3125) (9 p.).
Wienk, H.L.J., Tishchenko, E., Belardinelli, R., Tomaselli, S., Dongre, R., Spurio, R., Folkers, G.E., Gualerzi, C.O. & Boelens, R. (2012). Structural Dynamics of Bacterial Translation Initiation Factor IF2. Journal of Biological Chemistry, 287 (14), (pp. 10922-10932) (19 p.).
  2011 - Scholarly publications
de Graaf, A.J., Boere, K.W.M., Kemmink, J., Fokkink, R.G., van Nostrum, C.F., Rijkers, D.T.S., van de Gucht, J., Wienk, H.L.J., Baldus, M., Mastrobattista, E., Vermonden, T. & Hennink, W.E. (2011). Looped structure of flowerlike Micelles revealed by 1H NMR relaxometry and light scattering. Langmuir, 27 (16), (pp. 9843-9848) (6 p.).
Lange, Anja, Hoeller, D., Wienk, H.L.J., Marcillat, O., Lancelin, J.-M. & Walker, O. (2011). NMR reveals a different mode of binding of the Stam2 VHS domain to ubiquitin and diubiquitin. Biochemistry, 50 (1), (pp. 48-62) (15 p.).
  2010 - Scholarly publications
Brondijk, T.H.C., de Ruiter, T., Ballering, J., Wienk, H., Lebbink, R.J., van Ingen, H., Boelens, R., Farndale, R.W., Meyaard, L. & Huizinga, E.G. (2010). Crystal structure and collagen-binding site of immune inhibitory receptor LAIR-1: unexpected implications for collagen binding by platelet receptor GPVI. Blood, 115 (7), (pp. 1364-1373) (10 p.).
  2009 - Scholarly publications
Huang, A., de Jong, R.N., Wienk, H.L.J., Winkler, G.S., Timmers, H.T.M. & Boelens, R. (2009). E2-c-Cbl recognition is necessary but not sufficient for ubiquitination activity. Journal of Molecular Biology, 385 (2), (pp. 507-519) (13 p.).
Romanuka, J., Folkers, G.E., Biris, N., Tishchenko, E., Wienk, H.L.J., Bonvin, A.M.J.J., Kaptein, R. & Boelens, R. (2009). Specificity and affinity of Lac repressor for the auxiliary operators O2 and O3 are explained by the structures of their protein–DNA complexes. Journal of Molecular Biology, 390 (3), (pp. 478-489) (12 p.).
Wienk, H.L.J., Lammers, I., Hotze, A.C.G., Wu, J., Wechselberger, R.W., Owens, R., Stammers, D.K., Stuart, D., Kaptein, R. & Folkers, G.E. (2009). The tandem zinc-finger region of human ZHX adopts a novel C2H2 zinc finger structure with a C-terminal extension. Biochemistry, 48 (21), (pp. 4431-4439) (9 p.).
  2008 - Scholarly publications
Harper, S.M., Wienk, H., Wechselberger, R.W., Bos, J.L., Boelens, R. & Rehmann, H. (2008). Structural dynamics in the activation of Epac. Journal of Biological Chemistry, 283 (10), (pp. 6501-6508) (8 p.).
van Ingen, H., van Schaik, F.M.A., Wienk, H., Ballering, J., Rehmann, H., Dechesne, A.C., Kruijzer, J.A.W., Liskamp, R.M.J., Timmers, H.T.M. & Boelens, R. (2008). Structural insight into the recognition of the H3K4me3 mark by the TFIID subunit TAF3. Structure with Folding & design, 16 (8), (pp. 1245-1256) (12 p.).
Stordeur, C., Dallüge, R., Birkenmeier, O., Wienk, H.L.J., Rudolph, R., Lange, C. & Lücke, C. (2008). The NMR solution structure of the artificial protein M7 matches the computationally designed model. Proteins: Structure, Function and Genetics, 72 (3), (pp. 1104-1107) (4 p.).
  2005 - Scholarly publications
Wienk, H.L.J., Tomaselli, S., Bernard, C., Spurio, R., Picone, D., Gualerzi, C.O. & Boelens, R. (2005). Solution structure of the C1-subdomain of Bacillus stearothermophilus translation initiation factor IF2. Protein Science, 14, (pp. 2461-2468) (8 p.).
  2004 - Scholarly publications
Betz, Marco, Löhr, Frank, Wienk, Hans & Rüterjans, Heinz (18.05.2004). Long-Range Nature of the Interactions between Titratable Groups in Bacillus agaradhaerens Family 11 Xylanase: pH Titration of B. agaradhaerens Xylanase. Biochemistry, 43 (19), (pp. 5820-5831) (12 p.).
Lin, Yi-Jan, Dancea, Felician, Löhr, Frank, Klimmek, Oliver, Pfeiffer-Marek, Stefania, Nilges, Michael, Wienk, Hans, Kröger, Achim & Rüterjans, Heinz (17.02.2004). Solution Structure of the 30 kDa Polysulfide-Sulfur Transferase Homodimer from Wolinella succinogenes. Biochemistry, 43 (6), (pp. 1418-1424) (7 p.).
  2003 - Scholarly publications
Wienk, Hans, Maneg, Oliver, Lücke, Christian, Pristovšek, Primož, Löhr, Frank, Ludwig, Bernd & Rüterjans, Heinz (27.05.2003). Interaction of cytochrome c with cytochrome c oxidase: An NMR study on two soluble fragments derived from Paracoccus denitrificans. Biochemistry, 42 (20), (pp. 6005-6012) (8 p.).
Rogov, Vladimir V., Lücke, Christian, Muresanu, Lucia, Wienk, Hans, Kleinhaus, Ioana, Werner, Karla, Löhr, Frank, Pristovšek, Primož & Rüterjans, Heinz (01.12.2003). Solution structure and stability of the full-length excisionase from bacteriophage HK022. Biochemistry International, 270 (24), (pp. 4846-4858) (13 p.).
  2000 - Scholarly publications
Wijngaard, P., Demmers, J.A.A., Thompson, S.J., Wienk, H.L.J., de Kruijff, B. & Vredenberg, W.J. (2000). Further analysis of the involvement of the envelope anion channel PIRAC in chlorplast protein import. Biochemistry International, 267, (pp. 3812-3817) (5 p.).
Wienk, Hans L. J., Wechselberger, Rainer W., Czisch, Michael & De Kruijff, Ben (18.07.2000). Structure, dynamics, and insertion of a chloroplast targeting peptide in mixed micelles. Biochemistry, 39 (28), (pp. 8219-8227) (9 p.).
  1999 - Scholarly publications
Wienk, Hans L. J. & De Kruijff, Ben (01.12.1999). Expression, isolation, and characterization of a chloroplast targeting peptide. Protein Expression and Purification, 17 (3), (pp. 345-350) (6 p.).
Wienk, Hans L.J., Czisch, Michael & De Kruijff, Ben (25.06.1999). The structural flexibility of the preferredoxin transit peptide. FEBS Letters, 453 (3), (pp. 318-326) (9 p.).
  1996 - Scholarly publications
Robijn, Gerard W., Wienk, Hans L. J., Van Den Berg, Dick J. C., Haas, Han, Kamerling, Johannis P. & Vliegenthart, Johannes F. G. (14.05.1996). Structural studies of the exopolysaccharide produced by Lactobacillus paracasei 34-1. Carbohydrate Research, 285, (pp. 129-139) (11 p.).
  1995 - Scholarly publications
Pilon, Marinus, Wienk, Hans, Sips, Wendy, De Swaaf, Martin, Talboom, Irvin, Van 'T Hof, Ron, De Korte-Kool, Gerda, Demel, Rudy, Weisbeek, Peter & De Kruijff, Ben (24.02.1995). Functional domains of the ferredoxin transit sequence involved in chloroplast import. Journal of Biological Chemistry, 270 (8), (pp. 3882-3893) (12 p.).
  1994 - Scholarly publications
Rietveld, Ton G., Chupin, Vladimir V., Koorengevel, Martijn C., Wienk, Hans L.J., Dowhan, William & De Kruijff, Ben (18.11.1994). Regulation of lipid polymorphism is essential for the viability of phosphatidylethanolamine-deficient Escherichia coli cells. Journal of Biological Chemistry, 269 (46), (pp. 28670-28675) (6 p.).
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Gegenereerd op 2018-09-22 19:02:09

A big part of my function as researcher relates to the application of solution state NMR techniques on a diversity, mainly biologically relevant systems. Here, focus lies on the determination of structure, function and dynamics of proteins.



01.09.2015 to 30.08.2019
General project description 

The project iNEXT aims to give researchers integrated access to experimental structural biology approaches such as NMR, electron microscopy and X-ray technologies to determine new structures and functions of proteins and their complexes. The project is funded by the European Union with 10 million Euro from its Horizon 2020 Research and Innovation programme to enable European researchers to use unique, state-of-the-art infrastructures for structural biology from 23 research institutes in Europe. iNEXT is coordinated from the Netherlands by prof. Rolf Boelens (Utrecht University) with dr. Anastassis Perrakis (Netherlands Cancer Institute) as deputy coordinator.

Role Researcher & Contact Funding
EU grant
Project members UU
External project members:
  • European Molecular Biology Laboratory
  • Diamond Light Source
  • Consorzio Interuniversitario Risonanze Magnetiche di Metallo Proteine
  • Goethe University Frankfurt
  • The Netherlands Cancer Institute
  • Masaryk University / CEITEC
  • Spanish National Research Council
  • Leibniz-Institut für Molekulare Pharmakologie
  • Synchrotron SOLEIL
  • Lund University / MAXIV
  • Leiden University / NeCEN
  • Leiden University Medical Center
  • European Synchrotron Radiation Facility
  • Centre National de la Recherche Scientifique / RALF-NMR
  • ESFRI Instruct
  • Aarhus University
  • European Spallation Source
  • Eötvös Loránd University
  • Instituto de Tecnologia Química e Biológica
  • University of Oulu
  • University of Patras
  • Weizmann Institute of Science
01.06.2013 to 30.06.2021
General project description 

The project uNMR-NL was awarded 18,5 million euro from NWO to be established as a large-scale research facility for ultra-high field NMR spectroscopy (1.2 GHz). In the new uNMR-NL facility, researchers will use magnetic nuclear resonance (NMR) and MR Imaging (MRI) to study the origin of diseases like Alzheimer’s and Parkinson’s, to identify new potential drugs, to develop new materials for batteries and solar cells and to enhance the production of crops and food quality. The project is coordinated by prof. Marc Baldus of Utrecht University and also involves the Radboud University NIjmegen, Leiden University, Wageningen University,  Eindhoven University of Technology and the public-private partnership for the analytical sciences, COAST.

Role Researcher & Contact Funding
NWO grant
Project members UU
External project members:
  • prof. dr. Arno Kentgens (RU)
  • prof. dr. Marcellus Ubbink (LU)
  • prof. dr. Huub de Groot (LU)
  • prof. dr. John van Duynhoven (WUR)
  • prof. dr. Aldrik Velders (WUR)
  • dr. Henk van As (WUR)
  • Klaas Nicolay (TU/e)
  • Oscar van den Brink (COAST)

Completed projects

Bio-NMR 01.09.2010 to 31.08.2014
General project description

Bio-NMR is a project financed by the European Commission’s Framework Programme 7 (FP7) that aims to further the structuring of the Biological NMR infrastructures, their user community and biological NMR research in Europe into a coherent research community prepared to tackle scientific and biomedical challenges of increasing complexity at the forefront of research worldwide. Bio-NMR involves a comprehensive group of top NMR research infrastructures (RI) providing access in Europe and related stakeholders. Bio-NMR completely subsidizes access by European scientists of the instrumentation and expertise available at the partner infrastructures. 

Role Researcher & Contact Funding
EU grant
Project members UU
Gegenereerd op 2018-09-22 19:02:09
Additional functions and activities

No additional functions

Gegenereerd op 2018-09-22 19:02:09
Full name
dr. H.L.J. Wienk Contact details

Visiting address:
Nicolaas Bloembergengebouw, room 1.15
Padualaan 8, 3584 CH Utrecht
Accessible via Kruyt Building --> first floor East --> bridge to Sjoerd Groenman Building --> left
See http://www.uu.nl/en/research/nmr/contact

Postal address:
Utrecht University, NMR
Padualaan 8, 3584 CH Utrecht

Phone: +31 30 253 9928

Phone (secr): +31 30 253 2652



Gegenereerd op 2018-09-22 19:02:09
Last updated 20.08.2018