prof. dr. H.A.B. (Han) Wosten
H.A.B.Wosten@uu.nl
Gegenereerd op 2018-07-23 19:46:43


Chair
Microbiology
Profile

Han Wösten is hoogleraar Microbiologie, Onderwijsdirecteur Biologie en Biosciences

Han Wösten onderzoekt groei en ontwikkelingsprocessen in schimmels. De eerste onderzoekslijn richt zich op heterogeniteit in en tussen mycelia. Dit wordt met name in Aspergillus onderzocht. Het blijkt dat een mycelium niet een verzameling is van schimmeldraden die allemaal dezelfde functie en samenstelling hebben. In tegendeel, het blijkt dat er verschillende typen draden zijn, wat zich uit in een verschillende samenstelling van het cytoplasma. Het doel van deze heterogeniteit en het mechanisme hierachter wordt op dit moment onderzocht. Onlangs hebben we aangetoond dat celorganellen, die Woronin bodies worden genoemd, compartimenten binnen en tussen schimmeldraden kunnen afsluiten. Hierdoor wordt voorkomen dat het cytoplasma wordt gemengd en dat heterogeniteit blijft bestaan.  De tweede onderzoekslijn richt zich op de vorming van paddenstoelen. We bestuderen welke regulatiegenen betrokken zijn bij paddenstoelvormende schimmels, zoals de champignon en het waaiertje, en welke genen zij aansturen. Daarnaast kijken we naar de invloed van milieufactoren en hoe die door de paddenstoelvormende schimmels worden waargenomen. Onlangs hebben we een blauwlicht receptor geïdentificeerd in het waaiertje. Indien we het coderende gen inactiveerden dan werd het mycelium blind en kon het geen paddenstoelen meer vormen.

 

Onderzoeksprijzen

-Unilever Research prijs 1989 (1800 €)                                   

-Nederlandse Vereniging voor Biochemie en Moleculaire Biologie Prijs 1999 (1500 €)

-STW PIONIER 2001 (900.000 €)

-STW Simon Stevin Meester 2008 (500.000 €)

 

Documentaire: Schimmels, het plastic van de toekomst

Gegenereerd op 2018-07-23 19:46:43
All publications
  2018 - Scholarly publications
Segers, Frank J J, Wösten, Han A B & Dijksterhuis, Jan (2018). Aspergillus niger mutants affected in conidial pigmentation do not have an increased susceptibility to water stress during growth at low water activity. Letters in Applied Microbiology, 66 (3), (pp. 238-243). This article is protected by copyright. All rights reserved..
Escobar, Natalia, Valdes, Ivan D, Keizer, Esther M, Ordonez, Soledad R, Ohm, Robin A, Wösten, Han A B & de Cock, Hans (13.07.2018). Expression profile analysis reveals that Aspergillus fumigatus but not Aspergillus niger makes type II epithelial lung cells less immunological alert. BMC Genomics, 19 (1), (pp. 534).
Appels, Freek V W, Dijksterhuis, Jan, Lukasiewicz, Catherine E, Jansen, Kaspar M B, Wösten, Han A B & Krijgsheld, Pauline (2018). Hydrophobin gene deletion and environmental growth conditions impact mechanical properties of mycelium by affecting the density of the material. Scientific Reports, 8.
Gehrmann, Thies, Pelkmans, Jordi F, Ohm, Robin A, Vos, Aurin M, Sonnenberg, Anton S.M., Baars, Johan J.P., Wösten, Han A B, Reinders, Marcel J T & Abeel, Thomas (2018). Nucleus-specific expression in the multinuclear mushroom-forming fungus reveals different nuclear regulatory programs. Proceedings of the National Academy of Sciences of the United States of America, 115 (17), (pp. 4429-4434) (6 p.). Copyright © 2018 the Author(s). Published by PNAS..
Gerritsen, M G, Brinkman, P, Escobar Salazar, Natalia, Bos, L D, de Heer, K, Meijer, M, Janssen, H-G, de Cock, H, Wösten, H A B, Visser, C.E., van Oers, M H J & Sterk, P J (2018). Profiling of volatile organic compounds produced by clinical Aspergillus isolates using gas chromatography-mass spectrometry. Medical Mycology, 56 (2), (pp. 253–256) (4 p.). © The Author 2017. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com..
  2017 - Scholarly publications
Celis Ramirez, A.M., Wösten, HAB, Triana, Sergio, Restrepo, Silvia & de Cock, J.J.P.A. (06.11.2017). <i>Malassezia</i> spp. beyond The Mycobiota. SM Dermatology Journal, 3 (3) (10 p.).
Steinberg, Gero, Peñalva, Miguel A, Riquelme, Meritxell, Wosten, Han & Harris, Steven D (2017). Cell Biology of Hyphal Growth. Microbiology spectrum, 5 (2), (pp. 1-34) (34 p.).
Tegelaar, M. & Wösten, HAB (2017). Functional distinction of hyphal compartments. Scientific Reports, 7.
Wösten, HAB, Gurr, S.J., van Doorn, J., Luirink, J., van der Oost, J., Oudega, B., Robertson , L., Smit, H., Willemsen, P. & Biemans , C (2017). Fungi: friends and foes. In John van der Oost (Eds.), Mighty Microbes - The amazing world of microorganisms (pp. 103-105). Castricum: Stichting Microcanon.
Segers, Frank J J, van Laarhoven, Karel A, Wosten, Han & Dijksterhuis, Jan (2017). Growth of indoor fungi on gypsum. Journal of Applied Microbiology, 123 (2), (pp. 429–435). This article is protected by copyright. All rights reserved..
Vos, Aurin, Jurak, Edita, Pelkmans, Jordi, Herman, Koen, Pels, Gill, Baars, Johan J, Hendrix, Ed, Kabel, Mirjam A, Lugones, Luis G & Wosten, Han (2017). H2O2 as a candidate bottleneck for MnP activity during cultivation of Agaricus bisporus in compost. AMB Express, 7 (1).
Celis, A.M., Vos, Aurin, Triana, S., Medina, C.A., Escobar Salazar, Natalia, Restrepo, S., Wosten, Han & de Cock, Hans (01.03.2017). Highly efficient transformation system for Malassezia furfur and Malassezia pachydermatis using Agrobacterium tumefaciens-mediated transformation. Journal of Microbiological Methods, 134, (pp. 1-6) (6 p.).
Triana, Sergio, de Cock, Hans, Ohm, Robin A, Danies, Giovanna, Wösten, Han A B, Restrepo, Silvia, González Barrios, Andrés F & Celis Ramirez, Adriana (14.09.2017). Lipid Metabolic Versatility in Malassezia spp. Yeasts Studied through Metabolic Modeling. Frontiers in Microbiology, 8 (18 p.).
Vos, Aurin, Heijboer, Amber, Boschker, Henricus T. S., Bonnet, Barbara, Lugones, Luis & Wosten, Han (01.12.2017). Microbial biomass in compost during colonization of Agaricus bisporus. AMB Express, 7.
Benoit-Gelber, I, Gruntjes, T, Vinck, A, van Veluw, Jerre, Wosten, Han, Boeren, S, Vervoort, J J M & de Vries, R P (2017). Mixed colonies of Aspergillus niger and Aspergillus oryzae cooperatively degrading wheat bran. Fungal Genetics and Biology, 102, (pp. 31-37) (7 p.). Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved..
Wösten, HAB, Hoekstra, W. & de Graaf, Anje (2017). Schimmels; van plaag tot bouwmeester. (92 p.). Stichting Biowetenschapen en Maatschappij.
Aerts, David, Hauer, Esther E., Ohm, Robin A., Arentshorst, Mark, Teertstra, Wieke R., Phippen, Christopher, Ram, Arthur F J, Frisvad, Jens C. & Wösten, Han A. B. (30.09.2017). The FlbA-regulated predicted transcription factor Fum21 of Aspergillus niger is involved in fumonisin production. Antonie van Leeuwenhoek, (pp. 1-12) (12 p.).
Pelkmans, Jordi F, Patil, Mohini B, Gehrmann, Thies, Reinders, Marcel J T, Wösten, Han A B & Lugones, Luis G (2017). Transcription factors of Schizophyllum commune involved in mushroom formation and modulation of vegetative growth. Scientific Reports, 7 (1).
  2016 - Scholarly publications
Zacchetti, B, Willemse, J, Recter, B., van Dissel, D, van Wezel, GP, Wösten, HAB & Claessen, D (31.05.2016). Aggregation of germlings is a major contributing factor towards mycelial heterogeneity of Streptomyces. Scientific Reports, 6.
Pelkmans, J.F., Lugones, L.G. & Wosten, H.A.B. (16.01.2016). Fruiting Body Formation in Basidiomycetes. The Mycota - Growth, Differentiation and Sexuality (pp. 387-405) (18 p.).
Escobar, Natalia, Ordonez Alvarez, Soledad, Wosten, Han, Haas, Pieter-Jan A, de Cock, Hans & Haagsman, Henk P (2016). Hide, Keep Quiet, and Keep Low - Properties That Make Aspergillus fumigatus a Successful Lung Pathogen. Frontiers in Microbiology, 7.
van Leeuwen, M R, Wyatt, T T, van Doorn, T M, Lugones, L G, Wosten, Han & Dijksterhuis, J (2016). Hydrophilins in the filamentous fungus Neosartorya fischeri (Aspergillus fischeri) have protective activity against several types of microbial water stress. Environmental Microbiology Reports, 8 (1), (pp. 45-52). This article is protected by copyright. All rights reserved..
Teertstra, Wieke R., Tegelaar, Martin, Dijksterhuis, Jan, Golovina, Elena A., Ohm, Robin A. & Wösten, Han A.B. (21.12.2016). Maturation of conidia on conidiophores of Aspergillus niger. Fungal Genetics and Biology, 98, (pp. 61-70) (10 p.).
Gehrmann, Thies, Pelkmans, J.F., Lugones, L.G., Wösten, HAB, Abeel, Thomas & Reinders, Marcel J (23.09.2016). Schizophyllum commune has an extensive and functional alternative splicing repertoire. Scientific Reports, 6.
Segers, Frank J J, van Laarhoven, Karel, Huinink, Henk P, Adan, Olaf C, Wösten, HAB & Dijksterhuis, J. (15.08.2016). The Indoor Fungus Cladosporium halotolerans Survives Humidity Dynamics Markedly Better than Aspergillus niger and Penicillium rubens despite Less Growth at Lowered Steady-State Water Activity. Applied and Environmental Microbiology, 82 (17), (pp. 5089-5098).
Pelkmans, J.F., Vos, A.M., Scholtmeijer, K, Hendrix, Eddy, Baars, Johan J, Gehrmann, Thies, Reinders, Marcel J, Lugones, L.G. & Wösten, HAB (2016). The transcriptional regulator c2h2 accelerates mushroom formation in Agaricus bisporus. Applied Microbiology and Biotechnology, 100 (16), (pp. 7151-7159).
de Cock, J.J.P.A., van Doorn, J., van den Berg, L., Bitter, F.M. & Wösten, HAB (2016). Veilig werken met micro-organismen, parasieten en cellen in laboratoria en andere werkruimtes. Koninklijke Nederlandse Vereniging voor Microbiologie..
  2015 - Scholarly publications
Triana, Sergio, González, Andrés, Ohm, Robin A, Wosten, Han, de Cock, Hans, Restrepo, Silvia & Celis, Adriana (2015). Draft Genome Sequence of the Animal and Human Pathogen Malassezia pachydermatis Strain CBS 1879. Genome Announcements, 3 (5) (2 p.). Copyright © 2015 Triana et al..
Braem, Steven G E, Rooijakkers, Suzan H M, van Kessel, Kok P M, de Cock, Hans, Wosten, Han, van Strijp, Jos A G & Haas, Pieter-Jan A (10.02.2015). Effective Neutrophil Phagocytosis of Aspergillus fumigatus Is Mediated by Classical Pathway Complement Activation. Journal of Innate Immunity, (pp. 364-74) (11 p.).
Wang, Fengfeng, Krijgsheld, Pauline, Hulsman, Marc, de Bekker, Charissa, Müller, Wally H, Reinders, Marcel, de Vries, Ronald P & Wosten, Han (2015). FluG affects secretion in colonies of Aspergillus niger. Antonie van Leeuwenhoek, 107 (1), (pp. 225-40) (16 p.).
Tommassen, J.P.M. & Wosten, H.A.B. (2015). Microbial cell surfaces and secretion systems. In B. Lugtenberg (Eds.), Principles of plant-microbe interactions - Microbes for sustainable agriculture (pp. 33-44) (12 p.). Switserland: Springer International Publishing.
Bleichrodt, Robert-Jan, Hulsman, Marc, Wosten, H.A.B. & Reinders, Marcel J T (2015). Switching from a unicellular to multicellular organization in an Aspergillus niger hypha. mBio, 6 (2). Copyright © 2015 Bleichrodt et al..
Wang, Fengfeng, Dijksterhuis, Jan, Wyatt, Timon, Wösten, Han A B, Bleichrodt, Robert-Jan & Wosten, Han (2015). VeA of Aspergillus niger increases spore dispersing capacity by impacting conidiophore architecture. Antonie van Leeuwenhoek, 107 (1), (pp. 187-99) (13 p.).
  2014 - Scholarly publications
Wyatt, T T, van Leeuwen, M R, Dijksterhuis, J & Wosten, Han (2014). Mannitol is essential for the development of stress-resistant ascospores in Neosartorya fischeri (Aspergillus fischeri). Fungal Genetics and Biology, 64, (pp. 11-24) (14 p.). Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved..
Scholtmeijer, Karin, Cankar, Katarina, Beekwilder, Jules, Lugones, Luis G, Bosch, Dirk & Wosten, Han (2014). Production of (+)-valencene in the mushroom-forming fungus S. commune. Applied Microbiology and Biotechnology, 98 (11), (pp. 5059-5068) (10 p.).
Petrus, Marloes L C, van Veluw, G Jerre, Wösten, Han A B, Claessen, Dennis & Wosten, Han (2014). Sorting of Streptomyces cell pellets using a complex object parametric analyzer and sorter. J Biomed, 84 (6 p.).
Levasseur, Anthony, Lomascolo, Anne, Chabrol, Olivier, Ruiz-Dueñas, Francisco J, Boukhris-Uzan, Eva, Piumi, François, Kües, Ursula, Ram, Arthur F J, Murat, Claude, Haon, Mireille, Benoit, Isabelle, Arfi, Yonathan, Chevret, Didier, Drula, Elodie, Kwon, Min Jin, Gouret, Philippe, Lesage-Meessen, Laurence, Lombard, Vincent, Mariette, Jérôme, Noirot, Céline, Park, Joohae, Patyshakuliyeva, Aleksandrina, Sigoillot, Jean Claude, Wiebenga, Ad, Wösten, Han A B, Martin, Francis, Coutinho, Pedro M, de Vries, Ronald P, Martínez, Angel T, Klopp, Christophe, Pontarotti, Pierre, Henrissat, Bernard, Record, Eric & Wosten, Han (2014). The genome of the white-rot fungus Pycnoporus cinnabarinus - a basidiomycete model with a versatile arsenal for lignocellulosic biomass breakdown. BMC Genomics, 15.
  2013 - Scholarly publications
Berendsen, R.L., Schrier, N., Kalkhove, S.I.C., Lugones, L.G., Baars, J.J.P., Zijlstra, C., de Weerdt, M., Wösten, H.A.B. & Bakker, P.A.H.M. (2013). Absence of induced resistance in Agaricus bisporus against Lecanicillium fungicola. Antonie van Leeuwenhoek, 103, (pp. 539-550) (12 p.).
van Munster, J., Nitsche, B.M., Krijgsheld, P., Wijk, A., Dijkhuizen, L., Wösten, H.A.B., Ram, A.F.J. & Maarel, M.J.E.C. (2013). Chitinases CtcB and Cfcl modify the cell wall in sporulating aerial mycelium of Aspergillus niger. Microbiology, 159 (9), (pp. 1853-1867) (15 p.).
Bleichrodt, R., Vinck, A., Krijgsheld, P., van Leeuwen, M.R., Dijksterhuis, J & Wösten, H.A.B. (2013). Cytosolic streaming in vegetative mycelium and aerial structures of Aspergillus niger. Studies in Mycology, 74, (pp. 59-70) (12 p.).
Krijgsheld, P., Nitsche, B.M., Post, H., Levin, A.M., Muller, W.H., Heck, A.J.R., Ram, A.F., Altelaar, A.F.M. & Wösten, H.A.B. (2013). Deletion of flbA results in increased secretome complexity and reduced secretion heterogeneity in colonies of Aspergillus niger. Journal of Proteome Research, 12 (4), (pp. 1808-1819) (12 p.).
Berendsen, R.L., Kalkhove, S.I.C., Lugones, L.G., Baars, J.J., Wösten, H.A.B. & Bakker, P.A.H.M. (2013). Effects of the mushroom-volatile 1-octen-3-ol on dry bubble disease. Applied Microbiology and Biotechnology, 97 (12), (pp. 5535-5543) (9 p.).
Wyatt, T.T., Wösten, H.A.B. & Dijksterhuis, J. (2013). Fungal spores for dispersion in space and time. Advances in Applied Microbiology, 85, (pp. 43-91) (49 p.).
van Leeuwen, M.R., Krijgsheld, P., Bleichrodt, R., Menke, F.L.H., Stam, H., Stark, J., Wösten, H.A.B. & Dijksterhuis, J. (2013). Germination of conidia of Aspergillus niger is accompanied by major changes in RNA profiles. Studies in Mycology, (pp. 59-70) (12 p.).
van Veluw, G.J., Teertstra, W.R., de Bekker, A.M., Vinck, A., van der Beek, N., Muller, W.H., Arentshorst, M., van der Mei, H.C., Ram, A.F., Dijksterhuis, J. & Wösten, H.A.B. (2013). Heterogeneity in liquid shaken cultures of Aspergillus niger inoculated with melanised conidia or conidia of pigmentation mutants. Studies in Mycology, 74 (1), (pp. 47-57) (11 p.).
Wösten, H.A.B., van Veluw, G.J., de Bekker, A.M. & Krijgsheld, P. (2013). Heterogeneity in the mycelium: implications for the use of fungi as cell factors. Biotechnology Letters, 35 (8), (pp. 1155-1164) (10 p.).
Bleichrodt, R., van Veluw, G.J., Recter, B., Maruyama, J.-I., Kitamoto, K. & Wösten, H.A.B. (2013). Hyphal heterogeneity in Aspergillus oryze is the result of dynamic closure of septa by Woronin bodies. Molecular Microbiology, 86 (6), (pp. 1334-1344) (11 p.).
Berends, E., Lehle, L., Henquet, M., Hesselink, T., Wösten, H.A.B., Lugones, L.G. & Bosch, D. (2013). Identification of alg3 in the mushroom-forming fungus Schizophyllum commune and analysis of the delta-alg3 knockout mutant. Glycobiology, 23 (2), (pp. 147-154) (8 p.).
Berends, Elsa, Lehle, Ludwig, Henquet, Maurice, Hesselink, Thamara, Wösten, Han A.B., Lugones, Luis G. & Bosch, Dirk (01.02.2013). Identification of alg3 in the mushroom-forming fungus Schizophyllum commune and analysis of the Δalg3 knockout mutant. Glycobiology, 23 (2), (pp. 147-154) (8 p.).
Knaus, H., Blab, G., van Veluw, G.J., Gerritsen, H.C. & Wösten, H.A.B. (2013). Label-free fluorescence microscopy in fungi. Fungal Biology Reviews, 27 (2), (pp. 60-66) (7 p.).
Knaus, H., Blab, G., Agronskaia, A.V., van den Heuvel, D.J., Gerritsen, H.C. & Wösten, H.A.B. (2013). Monitoring the metabolic state of fungal hyphae and the presence of melanin by nonlinear spectral imaging. Applied and Environmental Microbiology, 79 (20), (pp. 6345-6350) (6 p.).
Akanbi, M.H., Post, E., van Putten, S.M., de Vries, L., Smisterova, J., Meter-Arkema, A., Wösten, H.A.B., Rink, R. & Scholtmeijer, K. (2013). The antitumor activity of hydrophobin SC3, a fungal protein. Applied Microbiology and Biotechnology, 97 (10), (pp. 4385-4392) (8 p.).
Ohm, R.A., Aerts, D., Wösten, H.A.B. & Lugones, L.G. (2013). The blue light receptor complex WC-1/2 of Schizophyllum commune is involved in mushroom formation and protection against phototoxicity. Environmental Microbiology, 15 (3), (pp. 943-955) (13 p.).
  2012 - Scholarly publications
Vankuyk, P.A., Benen, J.A.E., Wosten, H.A.B., Visser, J. & de Vries, R.P. (2012). A broader role for AmyR in Aspergillus niger: regulation of the utilisation of D:-glucose or D:-galactose containing oligo- and polysaccharides. Applied Microbiology and Biotechnology, 93, (pp. 285-293) (9 p.).
van Veluw, G.J., Petrus, M., Gubbens, J., de Graaf, R., de Jong, I.P., van Wezel, G.P., Wösten, H.A.B. & Claessen, D. (2012). Analysis of two distinct mycelial populations in liquid-grown Streptomyces cultures using a flow cytometry-based proteomics approach. Applied Microbiology and Biotechnology, 96, (pp. 1301-1312) (12 p.).
Berendsen, R.L., Kalkhove, S.I.C., Lugones, L.G., Baars, J.J.P. & Wösten, H.A.B. (2012). Effects of fluorescent Pseudomonas spp. isolated from mushroom cultures on Lecanicillium fungicola. Biological Control, 63, (pp. 210-221) (12 p.).
Morin, E., Kohler, A., Baker, A.R., Foulogne-Oriol, M., Lombard, V., Nagy, L.G., Ohm, R.A., Patyshakuliyeva, A, Brun, A., Aerts, A.L., Billette, C., Coutinho, P.M., Deakin, G., Doddapaneni, H., Floudas, D., Grimwood, J., Hildén, K., Kües, U., Labutti, K.M., Lapidus, A., Lindquist, E.A., Lucas, S.M., Murat, C., Riley, R.W., Salamov, A.A., Schmutz, J., Subramanian, V., Wösten, H.A.B., Xu, J., Eastwood, D.C., Foster, G.D., Sonnenberg, A.S., Cullen, D., de Vries, R.P., Lundell, T., Hibbett, D.S., Henrissat, B., Burton, K.S., Kerrigan, R.W., Challen, M.P., Grigoriev, I.V. & Martin, F. (2012). Genome sequence of the button mushroom Agaricus bisporus reveals mechanisms governing adaptation to a humic-rich ecological niche. Proceedings of the National Academy of Sciences of the United States of America, 109, (pp. 17501-17506) (6 p.).
Berendsen, R.L., Kalkhove, S.I.C., Lugones, L.G., Wösten, H.A.B. & Bakker, P.A.H.M. (2012). Germination of Lecanicillium fungicola in the mycosphere of Agaricus bisporus. Environmental Microbiology Reports, (pp. 227-233) (7 p.).
de Jong, W., Vijgenboom, E., Dijkhuizen, L., Wösten, H.A.B. & Claessen, D. (2012). SapB and the rodlins are required for development of Streptomyces coelicolor in high osmolarity media. FEMS Microbiology Letters, 329, (pp. 154-159) (6 p.).
Krijgsheld, P., Wosten, H.A.B., Altelaar, A.F.M., Post, H., Ringrose, J.H., Muller, W.H. & Heck, A.J.R. (30.03.2012). Spatially resolving the microtome within the mycelium of the cell factory Aspergillus niger. onbekend Philipps-Universitaet Marburg, Germany, 11th European Conference on Fungal Genetics.
Krijgsheld, P., Altelaar, A.F.M., Post, H., Ringrose, J.H., Muller, W.H., Heck, A.J.R. & Wösten, H.A.B. (2012). Spatially resolving the secretome within the mycelium of the cell factory Aspergillus niger. Journal of Proteome Research, 11 (5), (pp. 2807-2818) (12 p.).
van Leeuwen, M.R., Krijgsheld, P., Wyatt, T.T., Golovina, E.A., Menke, H., Dekker, A., Stark, J., Stam, H., Bleichrodt, R., Wösten, H.A.B. & Dijksterhuis, J (2012). The effect of natamycin on the transcriptome of conidia of Aspergillus niger. Studies in Mycology, 74, (pp. 71-85) (15 p.).
  2011 - Scholarly publications
Teertstra, W.R., Krijgsheld, P. & Wösten, H.A.B. (2011). Absence of repellents in Ustilago maydis induces genes encoding small secreted proteins. Antonie van Leeuwenhoek, 100 (2), (pp. 219-229) (11 p.).
Battaglia, E., Visser, L., Nijssen, A., van Veluw, G.J., Wösten, H.A.B. & de Vries, R.P. (2011). Analysis of regulation of pentose utilisation in Aspergillus niger reveals evolutionary adaptations in Eurotiales. Studies in Mycology, 69 (1), (pp. 31-38) (8 p.).
Benoit, Isabelle, Honglí, Hu, Van Den Brink, J., Gruben, Birgit, Wosten, H. & De Vries, R. (01.01.2011). Co-cultivations of fungi improve the production of specific enzymes. Acta Microbiologica et Immunologica Hungarica, 58, (pp. 126) (1 p.).
de Bekker, A.M., van Veluw, G.J., Vinck, A. & Wösten, H.A.B. (2011). Heterogeneity of Aspergillus niger microcolonies in liquid shaken cultures. Applied and Environmental Microbiology, 77 (4), (pp. 1263-1267) (5 p.).
Vinck, A., de Bekker, A.M., Ossin, A., Ohm, R.A., de Vries, R.P. & Wösten, H.A.B. (2011). Heterogenic expression of genes encoding secreted proteins at the periphery of Aspergillus niger colones. Environmental Microbiology, 13 (1), (pp. 216-225) (10 p.).
Berends, E., Schmidt, K., Lugones, L., Wosten, H. & Bosch, D. (01.07.2011). Mushroom glycoengineering for the production of therapeutic glycoproteins. Glycoconjugate Journal, 28 (5), (pp. 239-240) (2 p.).
de Bekker, A.M., Bruning, O., Jonker, M.J., Breit, T.M. & Wösten, H.A.B. (2011). Single cell transcriptomics of neighboring hyphae of Aspergillus niger. Genome Biology, 12 (8), (pp. R71) (1 p.).
Ohm, R.A., de Jong, J.F., de Bekker, A.M., Wösten, H.A.B. & Lugones, L.G. (2011). Transcription factor genes of Schizophyllum commune involved in regulation of mushroom formation. Molecular Microbiology, 81 (6), (pp. 1433-1445) (13 p.).
  2011 - Popularising publications
Wösten, H.A.B. (2011). Schimmels; een groot netwerk van veelzijdigheid. In H. Smit, W. Reijnders, J. van Doorn, J. van der Oost & P. Willemsen (Eds.), Natuurwetenschap en Techniek (pp. 198-200) (3 p.). Veen Magazines BV.
Wösten, H.A.B. (2011). Voorwoord. De Microcanon. In H. Smit, W. Reijnders, J. van Doorn, J. van der Oost & P. Willemsen (Eds.), Natuurwetenschap en Techniek (pp. VIII-XI). Veen Magazines BV.
  2010 - Scholarly publications
Ohm, R.A., de Jong, J.F., Berends, E., Wang, F., Wösten, H.A.B. & Lugones, L.G. (2010). An efficient gene deletion procedure for the mushroom-forming basidiomycete Schizophyllum commune. Mircen Journal of Applied Microbiology and Biotechnology
Zampieri, F., Wösten, H.A.B. & Scholtmeijer, K. (2010). Creating surface properties using a palette of hydrophobins. Materials Chemistry and Physics, 3, (pp. 4607-4625) (19 p.).
Ohm, R.A., de Jong, J.F., Lugones, L.G., Aerts, A., Kothe, E., Stajich, J.E., de Vries, R.P., Record, E, Levasseur, A., Baker, S.E., Bartolomew, K.A., Coutinho, P.M., Erdmann, S., Fowler, T.J., Gathman, A.C., Lombard, V., Henrissat, B., Knabe, N., Kües, U., Lilly, W.W., Lindquist, E., Lucas, S., Magnuson, J.K., Piumi, F., Raudaskoski, M., Salamov, A., Schmutz, J., Schwarze, F.W., Vankuyk, P.A., Horton, J.S., Grigoriev, I.V. & Wösten, H.A.B. (2010). Genome sequence of the model mushroom Schizophillum commune. Nature Biotechnology, 28 (9), (pp. 957-963) (7 p.).
de Jong, J.F., Ohm, R.A., de Bekker, A.M., Wösten, H.A.B. & Lugones, L.G. (2010). Inactivation of ku80 in the mushroom-forming fungus Schizophyllum commune increases the relative incidence of homologous recombination. FEMS Microbiology Letters, 310 (1), (pp. 91-95) (5 p.).
Berendsen, R.L., Baars, J.J.P., Kalkhove, S.I.C., Lugones, L.G., Wösten, H.A.B. & Bakker, P.A.H.M. (2010). Pathogen profile: Lecanicillium fungicola, causal agent of dry bubble disease in white-button mushroom. Molecular Plant Pathology, 11, (pp. 585-595) (11 p.).
Aguilar-Osorio, G., Vankuyk, P.A., Seiboth, B., Solomon, P.S., Vinck, A., Kindt, F., Wösten, H.A.B. & de Vries, R.P. (2010). Spatial and developmental differentiation of mannitol dehydrogenase and mannitol-1-phosphate dehydrogenase in Aspergillus niger. Eukaryotic Cell, 9 (9), (pp. 1398-1402) (5 p.).
van Peer, A.F., Wang, F., van Driel, K.G.A., de Jong, J.F., van Donselaar, E.G., Muller, W.H., Boekhout, T., Lugones, L.G. & Wösten, H.A.B. (2010). The septal pore cap is an organelle that functions in vegetative growth and mushroom formation of the wood-rot fungus Schizophyllum commune. Environmental Microbiology, 12 (4), (pp. 833-844) (12 p.).
Haas Jimoh Akanbi, M., Post, E., Meter-Arkema, A., Rink, R, Robillard, G.T., Wang, X., Wösten, H.A.B. & Scholtmeijer, K. (2010). Use of hydrophobins in formulation of water insoluble drugs for oral administration. Colloids and Surfaces B: Biointerfaces, 75 (2), (pp. 526-531) (6 p.).
  2009 - Scholarly publications
Rutten, L., Ribot, C., Trejo-Aguilar, B., Pail, M., Wosten, H.A.B. & de Vries, R.P. (2009). A single amino acid change in the L-arabitol dehydrogenase (LadA) from Aspergillus niger broadens its substrate specificity and increases its activity. BMC Microbiology, 9, (pp. 166) (1 p.).
de Bekker, A.M., Wiebenga, L.A., Aguilar Osorio, J.G.D.J. & Wösten, H.A.B. (2009). An enzyme cocktail for efficent protoplast formation in Aspergillus niger. Journal of Microbiological Methods, 76 (3), (pp. 305-306) (2 p.).
Scholtmeijer, K., de Vocht, M.L., Rink, R., Robillard, G.T. & Wösten, H.A.B. (2009). Assembly of the fungal Sc3 hydrophobin into functional amyloid fibrils depends on its concentration and is promoted by cell wall polysaccharides. Journal of Biological Chemistry, 284 (39), (pp. 26309-26314) (6 p.).
de Jong, W., Wösten, H.A.B., Dijkhuizen, L. & Claessen, D. (2009). Attachment of Streptomyces coelicolor is mediated by amyloidal fimbriae that are anchored to the cell surface via cellulose. Molecular Microbiology, 73 (6), (pp. 1128-1140) (13 p.).
van Peer, A.F., Muller, W.H., Boekhout, T., Lugones, L.G. & Wösten, H.A.B. (2009). Cytoplasmic continuity revisited: closure of septa of the filamentous fungus Schizophyllum commune in response to environmental conditions. PLoS One, 4 (6), (pp. e5977) (1 p.).
Berends, E., Ohm, R.A., de Jong, J.F., Rouwendal, G.J.A., Wösten, H.A.B., Lugones, L.G. & Bosch, D. (2009). Genomic and biochemical analysis of N glycosylation in the mushroom-forming basidiomycete Schizophyllum commune. Applied and Environmental Microbiology, 75 (13), (pp. 4648-4652) (5 p.).
de Jong, W., Manteca, A., Sanchez, J., Bucca, G., Smith, C.P., Dijkhuizen, L., Claessen, D. & Wösten, H.A.B. (2009). NepA is a structural cell wall protein involved in maintenance of spore dormancy in Streptomyces coelicolor. Molecular Microbiology, 71 (6), (pp. 1591-1603) (13 p.).
van Peer, A.F., de Bekker, A.M., Vinck, A., Wösten, H.A.B. & Lugones, L.G. (2009). Pleomycin increases transformation efficiency and promotes single integrations in Schizophyllum commune. Applied Environmental Microbiology, 75 (5), (pp. 1243-1247) (5 p.).
Grijpstra, J., Gerwig, G.J., Wösten, H.A.B., Kamerling, J.P. & de Cock, J.J.P.A. (2009). Production of extracellular polysaccharides by cap mutants of Cryptococcus neoformans. Eukaryotic Cell, 8 (8), (pp. 1165-1173) (9 p.).
Berendsen, R.L., Baars, J, Kalkhove, S., Wadman, M.W., Lugones, L.G., Wösten, H.A.B. & Bakker, P.A.H.M. (2009). Selection of Pseudomonas strains that are antagonistic to Verticillium fungicola, the causal agent of dry bubble disease in Agaricus bisporus. In B. Duffy, M. Maurhofer, C. Keel, C. Gessler, Y. Elad & S. Kiewnick (Eds.), Molecular tools for understanding and improving biocontrol. (pp. 32) (1 p.). IOBC.
Berends, E., Scholtmeijer, K., Wösten, H.A.B., Bosch, D. & Lugones, L.G. (2009). The use of mushroom-forming fungi for the production of N-glycosylated therapeutic proteins. Trends in Microbiology, 17 (10), (pp. 439-443) (5 p.).
Duranová, M., Spániková, S., Wösten, H.A.B., Biely, P. & de Vries, R.P. (2009). Two glucuronoyl esterases of Phanerochaete chrysosporium. Archives of Microbiology, 191 (2), (pp. 133-140) (8 p.).
  2008 - Scholarly publications
de Vries, R.P., de Lange, E.S., Wösten, H.A.B. & Stalpers, J. (2008). Fibulorhizoctonia psychrophila, a spoilage fungus of carrot, is a source of enzymes with high activity at low temperatures. Antonie van Leeuwenhoek, 93, (pp. 407-413) (7 p.).
van Driel, K.G.A., van Peer, A.F., Grijpstra, J., Wösten, H.A.B., Verkleij, A.J., Muller, W.H. & Boekhout, T. (2008). Septal pore cap protein SPC18, isolated from the basidiomycetous fungus Rhizoctonia solani, also resides in pore-plugs. Eukaryotic Cell, 7, (pp. 1865-18873) (9 p.).
  2007 - Scholarly publications
van Driel, Kenneth G A, van Peer, Arend F, Wosten, Han, Verkleij, Arie J, Boekhout, Teun & Müller, Wally H (2007). Enrichment of perforate septal pore caps from the basidiomycetous fungus Rhizoctonia solani by combined use of French press, isopycnic centrifugation, and Triton X-100. Journal of Microbiological Methods, 71 (3), (pp. 298-304) (7 p.).
Pel, H.J., Winde, H.J., Archer, D.B., Dyer, P.S., Hofmann, G., Schaap, P.J., Turner, G., de Vries, R.P., Albang, R., Albermann, K., Andersen, M.R., Bendtsen, J.D., Benen, J.A.E., van den Berg, M.., Breestraat, S., Caddick, M.X., Contreras, R., Cornell, M., Coutinho, P.M., Danchin, E.G., Debets, A.J., Dekker, P., van Dijck, P.W., van Dijk, A., Dijkhuizen, L., Driessen, A.J., d'Enfert, C., Geysens, S., Goosen, C., Groot, G.S., de Groot, P.W., Guillemette, T., Henrissat, B., Herweijer, M., van den Hombergh, J.P., van den Hondel, C.A.M.J.J., van der Heijden, R.T., Kaaij, R.M., Klis, F.M., Kools, H.J., Kubicek, C.P., van Kuyk, P.A., Lauber, J., Lu, X, Maarel, M.J., Meulenberg, R., Menke, H., Mortimer, M.A., Nielsen, J., Oliver, S.G., Olsthoorn, M., Pal, K., van Peij, N.N., Ram, A.F., Rinas, U., Roubos, J.A., Sagt, C.M.J., Schmoll, M., Sun, J., Ussery, D., Varga, J., Vervecken, W., van de Vondervoort, P.J., Wedler, H., Wösten, H.A.B., Zeng, A.P., Ooyen, A.J., Visser, J. & Stam, H. (2007). Genome sequencing and analysis of the versatile cell factory Aspergillus niger. Nature Biotechnology, 25 (2), (pp. 221-231) (11 p.).
de Jong, W., Claessen, D., Bucca, G., Smith, C., Dijkhuizen, L. & Wösten, H.A.B. (26.08.2007). Identification of novel cell wall located proteins involved in differentiation of Streptomyces coelicolor. In M. Goodfellow & A. Ward (Eds.), 14th International Symposium on the Biology af Actinomycetes (pp. 74-74) (1 p.). Newcastle, UK: University of Newcastle, 14th International Symposium on the Biology af Actinomycetes.
van Driel, K.G.A., Boekhout, T., Wösten, H.A.B., Verkleij, A.J. & Muller, W.H. (2007). Laser microdissection of fungal septa as visualized by scanning electron microscopy. Fungal Genetics and Biology, 44, (pp. 466-473) (8 p.).
Levin Chucrel, A.M., de Vries, R.P. & Wösten, H.A.B. (2007). Localization of protein secretion in fungal colonies using a novel culturing technique; the ring-plate system. Journal of Microbiological Methods, 69 (2), (pp. 399-401) (3 p.).
de Groot, M.J.L., van den Dool, C., Wösten, H.A.B., Levisson, M., Vankuyk, P.A., Ruijter, G.J.G. & de Vries, R.P. (2007). Regulation of pentose catabolic pathway genes of Aspergillus niger. Food Technology and Biotechnology, 45 (2), (pp. 134-138) (5 p.).
Levin Chucrel, A.M., de Vries, R.P., Conesa, A., de Bekker, A.M., Talon, M., Menke, H.H., van Peij, N.N. & Wösten, H.A.B. (2007). Spatial differentiation in the vegetative mycelium of Aspergillus niger. Eukaryotic Cell, 6 (12), (pp. 2311-2322) (12 p.).
  2006 - Scholarly publications
Kamper, J., Kahmann, R., Bolker, M., Ma, L.J., Brefort, T., Saville, B.J., Banuett, F., Kronstad, J.W., Gold, S.E., Muller, O., Perlin, M.H., Wösten, H.A.B., de Vries, R.P., Ruiz-Herrera, J., Reynaga-Pena, C.G., Snetselaar, K., McCann, M., Perez-Martin, J., Feldbrugge, M., Basse, C.W., Steinberg, G., Ibeas, J.I., Holloman, W., Guzman, P., Farman, M., Stajich, J.E., Sentandreu, R., Gonzalez-Prieto, J.M., Kennell, J.C., Molina, L., Schirawski, J., Mendoza-Mendoza, A., Greilinger, D., Munch, K., Rossel, N., Scherer, M., Vranes, M., Ladendorf, O., Vincon, V., Fuchs, U., Sandrock, B., Meng, S., Ho, E.C., Cahill, M.J., Boyce, K.J., Klose, J., Klosterman, S.J., Deelstra, H.J., Ortiz-Castellanos, L., Li, W., Sanchez-Alonso, P., Schreier, P.H., Hauser-Hahn, I., Vaupel, M., Koopmann, E., Friedrich, G., Voss, H., Schluter, T., Margolis, J., Platt, D., Swimmer, C., Gnirke, A., Chen, F., Vysotskaia, V., Mannhaupt, G., Guldener, U., Munsterkotter, M., Haase, D., Oesterheld, M., Mewes, H.W., Mauceli, E.W., DeCaprio, D., Wade, C.M., Butler, J., Young, S., Jaffe, D.B., Calvo, S., Nusbaum, C., Galagan, J. & Birren, B.W. (2006). Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis. Nature, 444, (pp. 97-101) (5 p.).
Askolin, S., Linder, M., Scholtmeijer, K, Tenkanen, M., Penttilä, M., de Vocht, M.L. & Wösten, H.A.B. (2006). Interaction and comparison of a class I hydrophobin from Schizophyllum commune and class II hydrophobins from Trichoderma reesei. Biomacromolecules, 7, (pp. 1295-1301) (7 p.).
Claessen, D., de Jong, W., Dijkhuizen, L. & Wösten, H.A.B. (2006). Regulation of Streptomyces development: reach for the sky!. Trends in Microbiology, 14, (pp. 313-314) (2 p.).
Teertstra, W.R., Deelstra, H.J., Vranes, M., Bohlmann, R., Kahmann, R., Kamper, J. & Wösten, H.A.B. (2006). Repellents have functionally replaced hydrophobins in mediating attachment to a hydrophobic surface and in formation of hydrophobic aerial hyphae in Ustilago maydis. Microbiology, 152, (pp. 3607-3612) (6 p.).
de Jong, J.F., Deelstra, H.J., Wösten, H.A.B. & Lugones, L.G. (2006). RNA-mediated gene silencing in monokaryons and dikaryons of Schizophyllum commune. Applied and Environmental Microbiology, 72, (pp. 1267-1269) (3 p.).
Wösten, H.A.B. & Wessels, J.G.H. (2006). The emergence of fruiting bodie in basidiomycentes. In U. Kues & R. Fisher (Eds.), The Mycota. Part 1: Growth, Differentiation and Sexuality (pp. 393-414) (22 p.). Berlin, Germany: Springer.
  2005 - Scholarly publications
Gebbink, M.F.B.G., Claessen, D., Bouma, B., Dijkhuizen, L. & Wösten, H.A.B. (2005). Amyloids - a functional coat for microorganisms. Nature Reviews Microbiology, 3, (pp. 333-341) (9 p.).
Vinck, A., Terlou, M., Pestman, W.R., Martens, E.P., Ram, A.F., van den Hondel, C.A.M.J.J. & Wösten, H.A.B. (2005). Fungi deploy specialized hyphea for waste processing. Nederlands Tijdschrift voor Medische Microbiologie, (pp. 58) (1 p.).
Vinck, A., Terlou, M., Pestman, W.R., Martens, E.P., Ram, A.F., van den Hondel, C.A.M.J.J. & Wösten, H.A.B. (2005). Hyphal differentiation in the exploring mycelium of Aspergillus niger. Molecular Microbiology, 58 (3), (pp. 693-699) (7 p.).
van Driel, K.G.A., van Peer, A.F., Wösten, H.A.B., Verkleij, A.J., Muller, W.H. & Boekhout, T. (2005). Isolation of septal pore caps from basidiomycetous fungi. Nederlands Tijdschrift voor Medische Microbiologie, (pp. 58) (1 p.).
Dijksterhuis, J, Samson, R., Wösten, H.A.B. & Lugones, L.G. (2005). Play, an abundant ascopore cell wall protein in Talaromyces macrosporus. Nederlands Tijdschrift voor Medische Microbiologie, (pp. 57) (1 p.).
Wang, X, Shi, F, Wösten, H.A.B., Hektor, H., Poolman, B. & Robillard, G.T. (2005). The SC3 hydrophobin self-assembles into a membrane with distinct mass transfer properties. Biophysical Journal, 88, (pp. 3434-3443) (10 p.).
Askolin, S., Penttila, M., Wösten, H.A.B. & Nakari-Setala, T. (2005). The Trichoderma reesei hydrophobin genes hfb1 and hfb2 have diverse functions in fungal development. FEMS Microbiology Letters October 13 [Epub ahead of print] 4th Dutch Endo-Neuro-Psycho meeting.
de Vries, R.P., van Grieken, C., Vankuyk, P.A. & Wösten, H.A.B. (2005). The value of genomic sequences in the rapid identification of genes encoding specific plant cell wall degrading enzymes. Current Genomics, 6, (pp. 157-187) (31 p.).
  2004 - Scholarly publications
Alves, A.M., Record, E, Lomascolo, A, Scholtmeijer, K., Asther, M, Wessels, J.G.H. & Wösten, H.A.B. (2004). Highly efficient production of laccase by the basidiomycete Pycnoporus cinnabarinus. Applied and Environmental Microbiology, 70, (pp. 6379-6384) (6 p.).
Teertstra, W.R., Lugones, L.G. & Wösten, H.A.B. (2004). In situ hybridisation in filamentous fungi using peptide nucleic acid probes. Fungal Genetics and Biology, 41, (pp. 1099-1103) (5 p.).
Wang, X, Permentier, HP, Rink, R, Kruijtzer, J.A.W., Liskamp, R.M.J., Wösten, H.A.B., Poolman, B & Robillard, G.T. (2004). Probing the self-assembly and the accompanying structural changes of hydrophobin SC3 on a hydrophobic surface by mass spectroscopy. Biophysical Journal, 87, (pp. 1919-1928) (10 p.).
Janssen, M.I., van Leeuwen, M.B.M., van Kooten, T.G., de Vries, J., Dijkhuizen, L. & Wösten, H.A.B. (2004). Promotion of fibroblast activity by coating with hydrophobins in the ß-sheet end state. Biomaterials, 25, (pp. 2731-2739) (9 p.).
Claessen, D., Wösten, H.A.B., Dijkhuizen, L. & van Keulen, G. (2004). Streptomyces coelicolor in an oxygen-limited liquid environment: adapt and escape. Microbiology Australia, 25, (pp. 22-23) (2 p.).
Claessen, D., Stokroos, I, Deelstra, H.J., Penninga, N.A., Bormann, C., Salas, J., Dijkhuizen, D & Wösten, H.A.B. (2004). The formation of the rodlet layer of streptomycetes is the result of the interplay between rodlins and chaplins. Molecular Microbiology, 53, (pp. 433-443) (11 p.).
Lugones, L.G., de Jong, J.F., de Vries, O.M.H., Jalving, R., Dijksterhuis, J & Wösten, H.A.B. (2004). The Sc15 protein of Schizophyllum commune mediates formation of aerial hyphae and attachment in the absence of the SC3 hydrophobin. Molecular Microbiology, 53, (pp. 707-716) (10 p.).
Scholtmeijer, K., Janssen, M.I., Leeuwen, M.B., van Kooten, T.G., Hektor, H. & Wösten, H.A.B. (2004). The use of hydrophobins to functionalize surfaces. Biomedical Material Engineering, 14, (pp. 447-454) (8 p.).
  2003 - Scholarly publications
Claessen, D., Rink, R., de Jong, W., Siebring, J., de Vreugd, P., Boersma, F.G.H., Dijkhuizen, L. & Wösten, H.A.B. (2003). A novel class of secreted hydrophobic proteins is involved in aerial hyphae formation in Streptomyces coelicolor by forming amyloid-like fibrils. Genes and Development, 17 (14), (pp. 1714-1726) (13 p.).
van Keulen, G., Jonkers, H., Claessen, D., Dijkhuizen, L. & Wösten, H.A.B. (2003). Differentiation and anaerobiosis in standing liquid cultures of Streptomyces coelicolor. Journal of Bacteriology, 185, (pp. 1455-1458) (4 p.).
Scholtmeijer, K & Wösten, H.A.B. (2003). Intron-dependent mRNA accumulation in filamentous fungi. Recent Research Developments in Molecular Microbiology (pp. 106-117) (12 p.). Kerala India: Research Signpost.
  2002 - Scholarly publications
Janssen, M.I., Leeuwen, M.B., Scholtmeijer, K., van Kooten, T.G., Dijkhuizen, L. & Wösten, H.A.B. (2002). Coating with genetic engineered hydrophobin promotes growth of fibroblasts on a hydrophobic solid. Biomaterials, 23 (24), (pp. 4847-4854) (8 p.).
de Vocht, M.L., Reviakine, I., Ulrich, W.P., Bergsma-Schutter, W., Wösten, H.A.B., Vogel, H., Brisson, A., Wessels, J.G. & Robillard, G.T. (2002). Self-assembly of the hydrophobin SC3 proceeds via two structural intermediates. Protein Science, 11 (5), (pp. 1199-1205) (7 p.).
Scholtmeijer, K., Janssen, M.I., Gerssen, B., de Vocht, M.L., van Leeuwen, B.M., van Kooten, T.G., Wösten, H.A.B. & Wessels, J.G. (2002). Surface modifications created by using engineered hydrophobins. Applied and Environmental Microbiology, 68 (3), (pp. 1367-1373) (7 p.).
Claessen, D., Wösten, H.A.B., van Keulen, G., Faber, O.G., Alves, A.M., Meijer, W.G. & Dijkhuizen, L. (2002). Two novel homologous proteins of Streptomyces coelicolor and Streptomyces lividans are involved in the formation of the rodlet layer and mediate attachment to a hydrophobic surface. Molecular Microbiology, 44 (6), (pp. 1483-1492) (10 p.).
  2002 - Other output
Wösten, H.A.B. (15.05.2002). Schimmels uit het sprookjesrijk. Utrecht: Universiteit Utrecht.
  2001 - Scholarly publications
Scholtmeijer, K., Wösten, H.A.B., Springer, J. & Wessels, J.G. (2001). Effect of introns and AT-rich sequences on expression of the bacterial hygromycin B resistance gene in the basidiomycete Schizophyllum commune. Applied and Environmental Microbiology, 67 (1), (pp. 481-483) (3 p.).
Scholtmeijer, K., Wessels, J.G. & Wösten, H.A.B. (2001). Fungal hydrophobins in medical and technical applications. Review. Applied Microbiology and Biotechnology, 56 (1-2), (pp. 1-8) (8 p.).
Wösten, H.A.B. (2001). Hydrophobins: multipurpose proteins. Annual Review of Microbiology, 55, (pp. 625-646) (22 p.).
  2000 - Scholarly publications
van Wetter, M.A., Wösten, H.A.B., Sietsma, J.H. & Wessels, J.G. (2000). Hydrophobin gene expression affects hyphal wall composition in Schizophyllum commune. Fungal Genetics and Biology, 31 (2), (pp. 99-104) (6 p.).
Wosten, Han & de Vocht, M L (18.09.2000). Hydrophobins, the fungal coat unravelled. Biochimica et Biophysica Acta, 1469 (2), (pp. 79-86) (8 p.).
van Wetter, M A, Wosten, Han & Wessels, J G (2000). SC3 and SC4 hydrophobins have distinct roles in formation of aerial structures in dikaryons of Schizophyllum commune. Molecular Microbiology, 36 (1), (pp. 201-10) (10 p.).
De Vocht, M.L., Reviakine, I., Wosten, H.A.B., Brisson, A., Wessels, J.G.H. & Robillard, G.T. (15.09.2000). Structural and functional role of the disulfide bridges in the hydrophobin SC3. Journal of Biological Chemistry, 275 (37), (pp. 28428-28432) (5 p.).
Wosten, Han & Willey, J M (2000). Surface-active proteins enable microbial aerial hyphae to grow into the air. Microbiology, 146 ( Pt 4), (pp. 767-73) (7 p.).
  1999 - Scholarly publications
Wosten, Han, van Wetter, M A, Lugones, L G, van der Mei, H C, Busscher, H J & Wessels, J G (28.01.1999). How a fungus escapes the water to grow into the air. Current Biology, 9 (2), (pp. 85-8) (4 p.).
Wosten, Han, Richter, M & Willey, J M (12.08.1999). Structural proteins involved in emergence of microbial aerial hyphae. Fungal Genetics and Biology, 27 (2-3), (pp. 153-60) (8 p.). Copyright 1999 Academic Press..
  1998 - Scholarly publications
Lugones, L G, Wosten, Han & Wessels, J G (1998). A hydrophobin (ABH3) specifically secreted by vegetatively growing hyphae of Agaricus bisporus (common white button mushroom). Microbiology, 144 ( Pt 8), (pp. 2345-53) (9 p.).
Tillotson, R D, Wosten, Han, Richter, M & Willey, J M (1998). A surface active protein involved in aerial hyphae formation in the filamentous fungus Schizophillum commune restores the capacity of a bald mutant of the filamentous bacterium Streptomyces coelicolor to erect aerial structures. Molecular Microbiology, 30 (3), (pp. 595-602) (8 p.).
de Vocht, M L, Scholtmeijer, K, van der Vegte, E W, Sonveaux, N, Wosten, Han, Ruysschaert, J M, Hadziloannou, G, Wessels, J G & Robillard, G T (1998). Structural characterization of the hydrophobin SC3, as a monomer and after self-assembly at hydrophobic/hydrophilic interfaces. Biophysical Journal, 74 (4), (pp. 2059-68) (10 p.).
  1997 - Scholarly publications
Wosten, Han & Wessels, J.G.H. (1997). Hydrophobins, from molecular structure to multiple functions in fungal development. Mycoscience, 38, (pp. 363-374) (12 p.).
  1996 - Scholarly publications
Van der Vegt, W., Van der Mei, H.C., Wosten, H.A.B., Wessels, J.G.H. & Busscher, H.J. (05.02.1996). A comparison of the surface activity of the fungal hydrophobin SC3p with those of other proteins. Biophysical Chemistry, 57 (2-3), (pp. 253-260) (8 p.).
Wosten, Han, Bohlmann, R, Eckerskorn, C, Lottspeich, F, Bölker, M & Kahmann, R (15.08.1996). A novel class of small amphipathic peptides affect aerial hyphal growth and surface hydrophobicity in Ustilago maydis. EMBO Journal, 15 (16), (pp. 4274-81) (8 p.).
  1995 - Scholarly publications
Wessels, J.G.H., Ásgeirsdóttir, S.A., de Vries, O.M.H., Lugones, L.G., Scheer, J.M.J., Schuren, F.H.J., Schuurs, T.A., van Wetter, M.A. & Wosten, Han (1995). Genetic regulation of emergent growth in Schizophyllum commune. Canadian Journal of Botany, 73, (pp. S273-S281) (9 p.).
Wosten, H.A.B., Ruardy, T.G., Van Der Mei, H.C., Busscher, H.J. & Wessels, J.G.H. (05.02.1995). Interfacial self-assembly of a Schizophyllum commune hydrophobin into an insoluble amphipathic protein membrane depends on surface hydrophobicity. Colloids and Surfaces B: Biointerfaces, 5 (3-4), (pp. 189-195) (7 p.).
  1994 - Scholarly publications
Wosten, H.A.B., De Vries, O.M.H., Van der Mei, H.C., Busscher, H.J. & Wessels, J.G.H. (05.02.1994). Atomic composition of the hydrophobic and hydrophilic membrane sides of self-assembled SC3p hydrophobin. Journal of Bacteriology, 176 (22), (pp. 7085-7086) (2 p.).
Wosten, Han, van der Mei, H C, Busscher, H J & Wessels, J G (1994). Atomic composition of the hydrophobic and hydrophilic membrane sides of self-assembled SC3p hydrophobin. Journal of Bacteriology, 176 (22), (pp. 7085-6) (2 p.).
Wosten, H.A.B., Asgeirsdottir, S.A., Krook, J.H., Drenth, J.H.H. & Wessels, J.G.H. (05.02.1994). The fungal hydrophobin Sc3p self-assembles at the surface of aerial hyphae as a protein membrane constituting the hydrophobic rodlet layer. European Journal of Cell Biology, 63 (1), (pp. 122-129) (8 p.).
  1993 - Scholarly publications
Moukha, S.M., Wosten, H.A.B., Asther, M. & Wessels, J.G.H. (05.02.1993). In situ localization of the secretion of lignin peroxidases in colonies of Phanerochaete chrysosporium using a sandwiched mode of culture. Journal of General Microbiology, 139 (5), (pp. 969-978) (10 p.).
De Vries, O.M.H., Fekkes, M.P., Wosten, H.A.B. & Wessels, J.G.H. (05.02.1993). Insoluble hydrophobin complexes in the walls of Schizophyllum commune and other filamentous fungi. Archives of Microbiology, 159 (4), (pp. 330-335) (6 p.).
Wosten, HAB. & Wessels, JGH. (1993). Interfacial Self-Assembly of a Fungal Hydrophobin into a Hydrophobic Rodlet Layer. Plant Cell, 5 (11), (pp. 1567-1574) (8 p.).
Moukha, S.M., Wosten, H.A.B., Mylius, E.-J., Asther, M. & Wessels, J.G.H. (05.02.1993). Spatial and temporal accumulation of mRNAs encoding two common lignin peroxidases in Phanerochaete chrysosporium. Journal of Bacteriology, 175 (11), (pp. 3672-3678) (7 p.).
  1991 - Scholarly publications
Wosten, H.A.B., Moukha, S.M., Sietsma, J.H. & Wessels, J.G.H. (05.02.1991). Localization of growth and secretion of proteins in Aspergillus niger. Journal of General Microbiology, 137 (8), (pp. 2017-2023) (7 p.).
Muller, W.H., Van Der Krift, T.P., Krouwer, A.J.J., Wosten, H.A.B., Van Der Voort, L., Smaal, E.B. & Verkleij, A.J. (05.02.1991). Localization of the pathway of the penicillin biosynthesis in Penicillium chrysogenum. EMBO Journal, 10 (2), (pp. 489-495) (7 p.).
  1990 - Scholarly publications
van der Lelie, D, Wosten, Han, Bron, S, Oskam, L & Venema, G (1990). Conjugal mobilization of streptococcal plasmid pMV158 between strains of Lactococcus lactis subsp. lactis. Journal of Bacteriology, 172 (1), (pp. 47-52) (6 p.).
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Gegenereerd op 2018-07-23 19:46:43
Project:
Research Through Design
01.07.2016 to 01.07.2019
General project description 

One of the challenges of this century is to transform our current economy into an eco-friendly and self-sustaining system. An innovative approach is the use of mycelium for the development of materials. Mycelium is an interwoven network of fungal filamentous cells called hyphae. Fungi form these mycelia on a wide variety of organic substrates. Mushroom forming fungi are known for their efficient colonization of ligno-cellulosic substrates like wood and straw. In a project of NWO Creative Industry we have developed sub-millimeter to centimeter thick layers of pure mycelium of a mushroom forming fungus. Depending on growth conditions and treatment, materials have been obtained that resemble paper, rubber, plastic and wood.


Composite biomaterials are achieved by growing mycelium of mushroom forming fungi organisms as Schizophyllym commune (split gill fungus), in a matrix of organic material such a s saw dust. The resulting materials resemble cork and wood and were used to make panels and objects. In this project, we aim to develop a palette of mycelium-based composite materials with different physical properties. Designers, artists, producers and end-users will explore the potential of the materials and will provide feedback how to improve the properties of the mycelium. This should result in tailor-made mycelia for innovative design solutions and new concepts of sustainable materials.

Role Researcher Funding
NWO grant: STW
Project members UU
External project members:
  • M. (Maurizio) Montalti Msc Design Academy Eindhoven (DEA)
  • dr. E. (Elvin) Karana Design Engineering Delft University of Technology (TUD)

Completed projects

Project:
Identity of compartments within a fungal hypha 01.06.2014 to 31.05.2018
General project description

Fungi form a mycelium of interconnected hyphae that grow and secrete at their tips and that branch sub-apically. Hyphae of most fungi are compartmentalized by septa that have pores with a diameter > 100 nm. These pores allow translocation of cytosol and even organelles within and between hyphae. Therefore, it was generally believed that the cytoplasm within a mycelium is continuous. This also explained why multiple compartments support the growth process at the hyphal tip. However, we have recently shown that septal pores can be closed in growing hyphae by means of specialized organelles. Closure is a dynamic process that is influenced by environmental conditions. This raises the questions whether compartments have their own molecular and functional identity, whether this identity results in functional synergism, and how closure of septal pores impacts these processes. These questions will be addressed in this proposal using Aspergillus niger as a model system. This fungus is widely used by the industry for the production of proteins and metabolites. We will use highly innovative technologies including life time imaging of protein translocation in hyphae and single cell expression analysis. In fact, we recently were the first who successfully adopted whole genome single cell expression analysis in a microbe. The results of this study are not only of fundamental importance, they may also provide insights how Aspergillus niger, and other fungi, can be improved as a cell factory.

 
Role Researcher Funding
NWO grant: NWO
Project members UU
External project members:
  • dr. R. B. J. Bleichrodt
Gegenereerd op 2018-07-23 19:46:43
Additional functions and activities
  • Voorzitter van de Koninklijke Nederlandse Vereniging voor Microbiologie (2009-2017)
  • Voorzitter van de sectie Mycologie van de Koninklijke Nederlandse Vereniging voor Microbiologie (2008-2017)
  • Lid van de Wetenschapsadviescommissie van het KNAW instituut Centraal Bureau voor Schimmelcultures (2001-2013)
  • Editor Antonie van Leeuwenhoek Journal of Microbiology (2005-heden)
  • Voorzitter of lid van diverse jury’s van NWO en STW programmas (2004-heden)
  • Consultant Procter & Gamble
Gegenereerd op 2018-07-23 19:46:43
Full name
prof. dr. H.A.B. Wosten Contact details
Hugo R. Kruytgebouw

Padualaan 8
Room W401
3584 CH  UTRECHT
The Netherlands


Phone number (direct) +31 30 253 3448
Phone number (department) +31 30 253 3084
Gegenereerd op 2018-07-23 19:46:43
Last updated 12.06.2018