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Dr. G.E. (Gert) Folkers

Assistant Professor 
Science
Chemistry
Biomolecular Sciences
NMR Spectroscopy
NMR Spectroscopy

Dr. G.E. (Gert) Folkers

Assistant Professor
NMR Spectroscopy
g.e.folkers@uu.nl
Profile Publications Teaching Ancillary activities Contact
Profile Publications Teaching Ancillary activities Contact

Publications

2021

Scholarly publications

le Paige, U. B., Xiang, S., Hendrix, M. M. R. M., Zhang, Y., Folkers, G. E., Weingarth, M., Bonvin, A. M. J. J., Kutateladze, T. G., Voets, I. K., Baldus, M., & van Ingen, H. (2021). Characterization of nucleosome sediments for protein interaction studies by solid-state NMR spectroscopy. Magnetic Resonance, 2(1), 187-202. https://doi.org/10.5194/mr-2-187-2021
Narasimhan, S., Pinto, C., Lucini Paioni, A., van der Zwan, J., Folkers, G. E., & Baldus, M. (2021). Characterizing proteins in a native bacterial environment using solid-state NMR spectroscopy. Nature protocols, 16, 893-918. https://doi.org/10.1038/s41596-020-00439-4

2020

Scholarly publications

Narasimhan, S., Folkers, G. E., & Baldus, M. (2020). When Small becomes Too Big: Expanding the Use of In-Cell Solid-State NMR Spectroscopy. ChemPlusChem, 85(4), 760-768. https://doi.org/10.1002/cplu.202000167

2019

Scholarly publications

Narasimhan, S., Scherpe, S., Lucini Paioni, A., van der Zwan, J., Folkers, G. E., Ovaa, H., & Baldus, M. (2019). Back Cover: DNP-Supported Solid-State NMR Spectroscopy of Proteins Inside Mammalian Cells (Angew. Chem. Int. Ed. 37/2019). Angewandte Chemie-International Edition, 58(37), 13162. https://doi.org/10.1002/anie.201908849
Narasimhan, S., Scherpe, S., Lucini Paioni, A., van der Zwan, J., Folkers, G. E., Ovaa, H., & Baldus, M. (2019). DNP‐Supported Solid‐State NMR Spectroscopy of Proteins Inside Mammalian Cells. Angewandte Chemie, 131(37), 13103-13107. https://doi.org/10.1002/ange.201903246
Narasimhan, S., Scherpe, S., Lucini Paioni, A., van der Zwan, J., Folkers, G. E., Ovaa, H., & Baldus, M. (2019). DNP-Supported Solid-State NMR Spectroscopy of Proteins Inside Mammalian Cells. Angewandte Chemie - International Edition, 58(37), 12969-12973. https://doi.org/10.1002/anie.201903246
Geng, C., Vangone, A., Folkers, G. E., Xue, L. C., & Bonvin, A. M. J. J. (2019). iSEE: Interface Structure, Evolution and Energy-based machine learning predictor of binding affinity changes upon mutations. Proteins: Structure, Function and Genetics, 87(2), 110-119. https://doi.org/10.1002/prot.25630

2018

Scholarly publications

Faridounnia, M., Folkers, G. E., & Boelens, R. (2018). Function and Interactions of ERCC1-XPF in DNA Damage Response. Molecules, 23(12), [3205]. https://doi.org/10.3390/molecules23123205

2017

Scholarly publications

Das, D., Faridounnia, M., Kovacic, L., Kaptein, R., Boelens, R., & Folkers, G. E. (2017). Single-stranded DNA Binding by the Helix-Hairpin-Helix Domain of XPF Protein Contributes to the Substrate Specificity of the ERCC1-XPF Protein Complex. Journal of Biological Chemistry, 292(7), 2842-2853. https://doi.org/10.1074/jbc.M116.747857
Cloin, B. M. C., De Zitter, E., Salas Pastene, D., Gielen, V., Folkers, G. E., Mikhaylova, M., Bergeler, M., Krajnik, B., Harvey, J., Hoogenraad, C. C., Van Meervelt, L., Dedecker, P., & Kapitein, L. C. (2017). Efficient switching of mCherry fluorescence using chemical caging. Proceedings of the National Academy of Sciences of the United States of America, 114(27), 7013-7018. https://doi.org/10.1073/pnas.1617280114

2016

Scholarly publications

Kaplan, M., Narasimhan, S., de Heus, C., Mance, D., van Doorn, S., Houben, K., Popov-Celeketic, D., Damman, R., Katrukha, E. A., Jain, P., Geerts, W. J. C., Heck, A. J. R., Folkers, G. E., Kapitein, L. C., Lemeer, S., van Bergen En Henegouwen, P. M. P., & Baldus, M. (2016). EGFR Dynamics Change during Activation in Native Membranes as Revealed by NMR. Cell, 167(5), 1241-1251.e11. https://doi.org/10.1016/j.cell.2016.10.038
Dongre, R., Folkers, G. E., Gualerzi, C. O., Boelens, R., & Wienk, H. (2016). A model for the interaction of the G3-subdomain of Geobacillus stearothermophilus IF2 with the 30S ribosomal subunit. Protein Science, 25(9), 1722-33. https://doi.org/10.1002/pro.2977

2015

Scholarly publications

Baker, L. A., Daniëls, M., Van Der Cruijsen, E. A. W., Folkers, G. E., & Baldus, M. (2015). Efficient cellular solid-state NMR of membrane proteins by targeted protein labeling. Journal of Biomolecular NMR, 62(2), 199-208. https://doi.org/10.1007/s10858-015-9936-5
Kaplan, M., Cukkemane, A., Van Zundert, G. C. P., Narasimhan, S., Daniëls, M., Mance, D., Waksman, G., Bonvin, A. M. J. J., Fronzes, R., Folkers, G. E., & Baldus, M. (2015). Probing a cell-embedded megadalton protein complex by DNP-supported solid-state NMR. Nature Methods, 12(7), 649-652. https://doi.org/10.1038/nmeth.3406
Faridounnia, M., Wienk, H., Kovacic, L., Folkers, G. E., Jaspers, N. G. J., Kaptein, R., Hoeijmakers, J. H. J., & Boelens, R. (2015). The Cerebro-oculo-facio-skeletal Syndrome Point Mutation F231L in the ERCC1 DNA Repair Protein Causes Dissociation of the ERCC1-XPF Complex. Journal of Biological Chemistry, 290(33), 20541-20555. https://doi.org/10.1074/jbc.M114.635169
Baker, L. A., Folkers, G. E., Sinnige, T., Houben, K., Kaplan, M., van der Cruijsen, E. A. W., & Baldus, M. (2015). Magic-Angle-Spinning Solid-State NMR of Membrane Proteins. Methods in Enzymology, 557, 307-328. https://doi.org/10.1016/bs.mie.2014.12.023

2014

Scholarly publications

Khan, F., Daniëls, M. A., Folkers, G. E., Boelens, R., Saqlan Naqvi, S. M., & Van Ingen, H. (2014). Structural basis of nucleic acid binding by Nicotiana tabacum glycine-rich RNA-binding protein: Implications for its RNA chaperone function. Nucleic Acids Research, 42(13), 8705-8718. https://doi.org/10.1093/nar/gku468
Kastritis, P. L., Garcia Lopes Maia Rodrigues, J., Folkers, G. E., Boelens, R., & Bonvin, A. M. J. J. (2014). Proteins feel more than they see: Fine-tuning of binding affinity by properties of the non-interacting surface. Journal of Molecular Biology, 426(14), 2632-2652. https://doi.org/10.1016/j.jmb.2014.04.017

2013

Scholarly publications

Wienk, H., Slootweg, J. C., Speerstra, S., Kaptein, R., Boelens, R., & Folkers, G. E. (2013). Solution structure of the helix hairpin helix domain of FAAP24 reveals that substrate specificity depends on an evolutionary conserved domain. Nucleic Acids Research, 41, 6739-6749.
Wienk, H. L. J., Slootweg, J. C., Speerstra, S., Kaptein, R., Boelens, R., & Folkers, G. E. (2013). The Fanconi anemia associated protein FAAP24 uses two substrate specific binding surfaces for DNA recognition. Nucleic Acids Research, 41(13), 6739-6749. https://doi.org/10.1093/nar/gkt354

2012

Scholarly publications

Das, D., Folkers, G. E., van Dijk, M., Jaspers, N. G. J., Hoeijmakers, J. H. J., Kaptein, R., & Boelens, R. (2012). The Structure of the XPF-ssDNA Complex Underscores the Distinct Roles of the XPF and ERCC1 Helix- Hairpin-Helix Domains in ss/ds DNA Recognition. Structure, 20(4), 667-675. https://doi.org/10.1016/j.str.2012.02.009
Wienk, H. L. J., Tishchenko, E., Belardinelli, R., Tomaselli, S., Dongre, R., Spurio, R., Folkers, G. E., Gualerzi, C. O., & Boelens, R. (2012). Structural Dynamics of Bacterial Translation Initiation Factor IF2. Journal of Biological Chemistry, 287(14), 10922-10932. https://doi.org/10.1074/jbc.M111.333393

2011

Scholarly publications

Busso, D., Peleg, Y., Folkers, G. E., & Celie, P. H. N. (2011). Expression of potein complexes using multiple E. coli protein co-expression systems: a benchmarking study. Journal of Structural Biology, 175, 159-170. https://doi.org/10.1016/j.jsb.2011.03.004
Berger, I., Blanco, A. G., Boelens, R., Cavarelli, J., Coll, M., Folkers, G. E., Nie, Y., Pogenberg, V., Schultz, P., Wilmanns, M., Moras, D., & Poterszman, A. (2011). Structural insights into transcription complexes. Journal of Structural Biology, 175(2), 135-146.
https://dspace.library.uu.nl/bitstream/handle/1874/213119/1304-main.pdf?sequence=1

2010

Scholarly publications

Huang, A., de Jong, R. N., Folkers, G. E., & Boelens, R. (2010). NMR characterization of foldedness for the production of E3 RING domains. Journal of Structural Biology, 172(1), 120-127. http://www.scopus.com/inward/record.url?eid=2-s2.0-77956191339&partnerID=40&md5=f9a97a5ca4bfb5afc84ffd6a8b5098bb
Bird, L. E., Ren, J., Nettleship, J. E., Folkers, G. E., Owens, RJ., & Stammers, D. K. (2010). Novel structural features in two ZHX homeodomains derived from a systematic study of single and multiple domains. BMC Structural Biology, 10(13), 1-15.
https://dspace.library.uu.nl/bitstream/handle/1874/202650/1472-6807-10-13.pdf?sequence=1

2009

Scholarly publications

Dadvar, P., Kovanich, D., Folkers, G. E., Rumpel, K., Raijmakers, R., & Heck, A. J. R. (2009). Phosphatidylethanolamine-binding proteins, including RKIP, exhibit affinity for phosphodiesterase-5 inhibitors. ChemBioChem, (10), 2654-2662.
Romanuka, J., Folkers, G. E., Biris, N., Tishchenko, E., Wienk, H. L. J., Bonvin, A. M. J. J., Kaptein, R., & Boelens, R. (2009). Specificity and affinity of Lac repressor for the auxiliary operators O2 and O3 are explained by the structures of their protein–DNA complexes. Journal of Molecular Biology, 390(3), 478-489. https://doi.org/10.1016/j.jmb.2009.05.022
Romanuka, J., van den Bulke, H., Kaptein, R., Boelens, R., & Folkers, G. E. (2009). Novel strategies to overcome expression problems encountered with toxic proteins: application to the production of Lac repressor proteins for NMR studies. Protein Expression and Purification, 67(2), 104-112.
Wienk, H. L. J., Lammers, I., Hotze, A. C. G., Wu, J., Wechselberger, R. W., Owens, R., Stammers, D. K., Stuart, D., Kaptein, R., & Folkers, G. E. (2009). The tandem zinc-finger region of human ZHX adopts a novel C2H2 zinc finger structure with a C-terminal extension. Biochemistry, 48(21), 4431-4439.

2008

Scholarly publications

Diercks, T., AB, E., Daniels, M. A., de Jong, R. N., Besseling, R., Kaptein, R., & Folkers, G. E. (2008). Solution structure and characterization of the DNA-binding activity of the B3BP–Smr domain. Journal of Molecular Biology, 383(5), 1156-1170.
https://dspace.library.uu.nl/bitstream/handle/1874/33233/543Supplementary%2520material.pdf?sequence=1
de Jong, R. N., Truffault, V. P. M., Diercks, T., AB, E., Daniels, M. A., Kaptein, R., & Folkers, G. E. (2008). Structure and DNA binding of the human Rtf1 Plus3 domain. Structure, 16(1), 149-159.
https://dspace.library.uu.nl/bitstream/handle/1874/33204/531.pdf?sequence=1
Das, D., Tripsianes, K., Jaspers, N. G. J., Hoeijmakers, J. H. J., Kaptein, R., Boelens, R., & Folkers, G. E. (2008). The HhH domain of the human DNA repair protein XPF forms stable homodimers. Proteins: Structure function and bioinformatics, 70(4), 1551-1563.

2007

Scholarly publications

Kamphuis, M. B., Monti, M. C., van den Heuvel, R. H. H., Santos-Sierra, S., Folkers, G. E., Lemonnier, M., Diaz-Orejas, R., Heck, A. J. R., & Boelens, R. (2007). Interactions between the toxin kid of the bacterial parD system and the antitoxins Kis and MazE. Proteins: Structure function and bioinformatics, 67, 219-231.
https://dspace.library.uu.nl/bitstream/handle/1874/26116/524.pdf?sequence=1
Tripsianes, K., Folkers, G. E., Zheng, C., Das, D., Grinstead, J. S., Kaptein, R., & Boelens, R. (2007). Analysis of the XPA and ssDNA-binding surfaces on the central domain of human ERCC1 reveals evidence for subfunctionalization. Nucleic Acids Research, 17, 5789-5798.
https://dspace.library.uu.nl/bitstream/handle/1874/26828/528.pdf?sequence=1

2006

Scholarly publications

de Jong, R. N., Daniëls, M., Kaptein, R., & Folkers, G. E. (2006). Enzyme free cloning for high throughput gene cloning and expression. Journal of Structural and Functional Genomics, 7(3-4), 109-118.
https://dspace.library.uu.nl/bitstream/handle/1874/23791/folkers_07_enzymefreecloning.pdf?sequence=1
AB, E., Atkinson, A. R., Banci, L., Bertini, I., Ciofi-Baffoni, S., Brunner, K., Diercks, T., Doetsch, V., Engelke, F., Folkers, G. E., Griesinger, C., Gronwald, W., Gunther, U., Habeck, M., de Jong, R. N., Kalbitzer, H. R., Kieffer, B., Leeflang, B. R., Loss, S., ... Kaptein, R. (2006). NMR in the SPINE Structural Proteomics project. Acta crystallographica. Section D, biological crystallography, D62, 1150-1161.
https://dspace.library.uu.nl/bitstream/handle/1874/20122/514.pdf?sequence=1
Grenha, R., Rzechorzek, N. J., Brannigan, J. A., de Jong, R. N., AB, E., Diercks, T., Truffault, V. P. M., Ladds, J. C., Fogg, M. J., Bongiorni, C., Perego, M., Kaptein, R., Wilson, K. S., Folkers, G. E., & Wilkinson, A. J. (2006). Structural Characterization of Spo0E-like Protein-aspartic Acid Phosphatases That Regulate Sporulation in Bacilli. Journal of Biological Chemistry, 281(49), 37993-38003.
https://dspace.library.uu.nl/bitstream/handle/1874/20124/516.pdf?sequence=1
Jonker, H. R. A., Wechselberger, R. W., Boelens, R., Kaptein, R., & Folkers, G. E. (2006). The intrinsically unstructured domain of PC4 modulates the activity of the structured core through inter- and intramolecular interactions. Biochemistry, 45(15), 5067-5081.
Jonker, H. R. A., Wechselberger, R. W., Pinkse, M. W. H., Kaptein, R., & Folkers, G. E. (2006). Gradual phosphorylation regulates PC4 coactivator function. The FEBS journal, 273, 1430-1444.
https://dspace.library.uu.nl/bitstream/handle/1874/19762/508.pdf?sequence=1
Au, K., Folkers, G. E., & Kaptein, R. (2006). Application of high-throughput technologies to a structural proteomics-type analysis of Bacillus anthracis. Acta crystallographica. Section D, biological crystallography, 62(10), 1267-1275.
https://dspace.library.uu.nl/bitstream/handle/1874/42779/fulltext.pdf?sequence=1
Alzari, P. M., & Folkers, G. E. (2006). Implementation of semi-automated cloning and and prokaryotic expression screening: the impact of SPINE. Acta crystallographica. Section D, biological crystallography, 62(10), 1103-1113.
https://dspace.library.uu.nl/bitstream/handle/1874/42781/fulltext.pdf?sequence=1
Berrow, N. S., & Folkers, G. E. (2006). Recombinant protein expression and solubility screening in Escherichia coli: a comparative study. Acta crystallographica. Section D, biological crystallography, 62(10), 1218-1226.
https://dspace.library.uu.nl/bitstream/handle/1874/42785/fulltext.pdf?sequence=1
de Jong, R. N., AB, E., Diercks, T., Truffault, V. P. M., Daniels, M. A., Kaptein, R., & Folkers, G. E. (2006). Solution Structure of the Human Ubiquitin-specific Protease 15 DUSP Domain. Journal of Biological Chemistry, 281(8), 5026-5031.
https://dspace.library.uu.nl/bitstream/handle/1874/20094/499.pdf?sequence=1
Milon, P., Tischenko, E. V., Tomsic, J., Caserta, E., Folkers, G. E., La Teana, A., Rodnina, M. V., Pon, C. L., Boelens, R., & Gualerzi, C. O. (2006). The nucleotide-binding site of bacterial translation initiation factor 2 (IF2) as a metabolic sensor. Proceedings of the National Academy of Sciences of the United States of America, 103(38), 13962-13967.

2005

Scholarly publications

Salinas, R. K., Folkers, G. E., Bonvin, A. M. J. J., Das, D., Boelens, R., & Kaptein, R. (2005). Altered specificity in DNA binding by the lac repressor: A mutant lac headpiece that mimics the gal repressor. ChemBioChem, 6(9), 1628-1637. https://doi.org/10.1002/cbic.200500049
Jonker, H. R. A., Wechselberger, R. W., Folkers, G. E., & Kaptein, R. (2005). Structural properties of the promiscuous VP16 activation domain. Biochemistry, 44(3), 827-839. https://doi.org/10.1021/bi0482912
Tripsianes, K., Folkers, G. E., AB, E., Das, D., Odijk, H., Jaspers, N. G. J., Hoeijmakers, J. H. J., Kaptein, R., & Boelens, R. (2005). The Structure of the Human ERCC1/XPF Interaction Domains Reveals a Complementary Role for the Two Proteins in Nucleotide Excision Repair. Structure, 13, 1849-1858.
https://dspace.library.uu.nl/bitstream/handle/1874/14818/493.pdf?sequence=1
Patterson, H. M., Brannigan, J. A., Cutting, S. M., Wilson, K. S., Wilkinson, A. J., AB, E., Diercks, T., de Jong, R. N., Truffault, V. P. M., Folkers, G. E., & Kaptein, R. (2005). The Structure of Bypass of Forespore C, an Intercompartmental Signaling Factor during Sporulation in Bacillus. Journal of Biological Chemistry, 280, 36214-36220.
https://dspace.library.uu.nl/bitstream/handle/1874/14817/492.pdf?sequence=1

2004

Scholarly publications

Dominguez, C., Folkers, G. E., & Boelens, R. (2004). RING domain proteins. In A. Messerschmidt (Ed.), Handbook of metalloproteins. Vol. 3 (pp. 338-350). Wiley.
Folkers, G. E., van Buuren, B. N. M., & Kaptein, R. (2004). Expression screening, protein purification and NMR analysis of human protein domains for structural genomics. Journal of Structural and Functional Genomics, 5(1-2), 119-131.

2002

Scholarly publications

Singh, S., Folkers, G. E., Bonvin, A. M. J. J., Boelens, R., Wechselberger, R. W., Niztayev, A., & Kaptein, R. (2002). Solution structure and DNA-binding properties of the C-terminal domain of UvrC from E.coli. EMBO Journal, 21(22), 6257-6266. https://doi.org/10.1093/emboj/cdf627
https://dspace.library.uu.nl/bitstream/handle/1874/42772/7594843a.pdf?sequence=1

2001

Scholarly publications

Kalodimos, C., Folkers, G. E., Boelens, R., & Kaptein, R. (2001). Strong DNA binding by covalently linked dimeric Lac headpiece: Evidence for the crucial role of the hinge helices. Proceedings of the National Academy of Sciences of the United States of America, 98(11), 6039-6044. http://www.pnas.org/
Folkers, G. E., Hanzawa, H., & Boelens, R. (2001). Zinc finger proteins. In Handbook on Metalloproteins (pp. 961-1000). Marcel Dether inc.

2000

Scholarly publications

Nooren, I. M., Folkers, G. E., Kaptein, R., Sauer, R. T., & Boelens, R. (2000). Structure and dynamics of the tetrameric mnt repressor and a model for its DNA complex. Journal of Biomolecular Structure and Dynamics, 17 Suppl 1, 113-22. https://doi.org/10.1080/07391102.2000.10506611
Van Tilborg, P. J. A., Czisch, M., Mulder, F. A. A., Folkers, G. E., Bonvin, A. M. J. J., Nair, M., Boelens, R., & Kaptein, R. (2000). Changes in dynamical behavior of the retinoid X receptor DNA-binding domain upon binding to a 14 base-pair DNA half site. Biochemistry, 39(30), 8747-8757. https://doi.org/10.1021/bi991550g

1999

Scholarly publications

Spronk, C. A., Folkers, G. E., Noordman, A. M., Wechselberger, R., van den Brink, N., Boelens, R., & Kaptein, R. (1999). Hinge-helix formation and DNA bending in various lac repressor-operator complexes. EMBO Journal, 18(22), 6472-80. https://doi.org/10.1093/emboj/18.22.6472
van Tilborg, PJA., Mulder, FAA., de Backer, MME., Nair, M., van Heerde, EC., Folkers, G., van der Saag, PT., Karimi-Nejad, Y., Boelens, R., & Kaptein, R. (1999). Millisecond to microsecond time scale dynamics of the retinoid X and retinoic acid receptor DNA-binding domains and dimeric complex formation. Biochemistry, 38(7), 1951-1956.
van Eijk, M. J., van Rooijen, M. A., Modina, S., Scesi, L., Folkers, G., van Tol, H. T., Bevers, M. M., Fisher, S. R., Lewin, H. A., Rakacolli, D., Galli, C., de Vaureix, C., Trounson, A. O., Mummery, C. L., & Gandolfi, F. (1999). Molecular cloning, genetic mapping, and developmental expression of bovine POU5F1. Biology of Reproduction, 60(5), 1093-103.

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