| December 2024- Present | Utrecht University |
Assistant professor at department of Pharmacy, UIPS, Utrecht University, Biomulecular Mass Spectrometry and Proteomics, The Netherlands Proteomics Center, Oncode Accelerator.
| 2019-End 2024 | Enara Bio (Oxford, UK) |
Director of mass spectrometry and head of antigen discovery:
• Identification and validation of a set of antigens shared across tumours for internal development of T-cell based therapies.
• Identification and validation of multiple antigens leading to external in partnerships, novel investments and creating high value clinical candidates for the company for a range of immunotherapies.
| 2016-2019 | Ludwig Cancer Institute (Lausanne, Switzerland) |
Post-Doctoral research:
• Implementation of an innovative, semi-automatized immunopeptidomics MS-based sample processing for analysis of HLA class I and II ligandomes.
• Insight into the HLA ligandome and the antigen processing and presentation machinery of APCs in response to differential stimuli for re-directing epitope presentation.
• Studying of mechanisms of immunogenicity of dendritic cell-based vaccination loaded with tumor lysates.
• MS-based antigen discovery of tumor tissues for cancer immunotherapy
| End 2011-2016 | Biomolecular Proteomics and Mass Spectrometry group (Utrecht) |
PhD/Post-Doctoral research:
• Study of proteasome-generated spliced peptide on HLA class I immunopeptidome.
• Characterization of the molecular binding mechanisms of phospho moieties harboring HLA B40:02 complexes.
• Study of the effect of Arginine methylation and dimethylation on HLA class I- peptide complexes.
• Quantitative profiling of monocytes stimulated with different vaccine adjuvants.
• Study of unexpected extended O-GlcNAc modifications on HLA class I-bound peptides.
• Establishment and characterization of a high performing 2D SCX-RP UHPLC-MS/MS platform for increasing to extend the proteome coverage of complex peptide mixtures.
• Development of a proteomic in vitro assay for identifying the consensus motifs of kinases.
• Mapping the HLA-DR ligandome of human monocyte-derived dendritic cells.
• Establishment of a complete proteomic workflow to study asymmetrical and symmetrical arginine dimethylation.
• Exploring different kind of workflows for middle-down proteomic approaches.