Dark quenchers, namely nonfluorescent quenching dyes, have been commonly applied in Förster resonance energy transfer (FRET) based fluorogenic probes for protease activity detection, nucleic acid hybridization and real-time polymerase chain reaction. In FRET-based systems, a specific quencher is normally able to quench the fluorescence only from those fluorophore donors that have significant overlap of their emission spectra with the absorption spectrum of the quencher when the donor and quencher are brought into proximity [1]

In our work, we will fabricate a FRET system choosing CuInS2/ZnS QDs as donor while dark dyes as acceptor by using water-soluble quencher, IRDye QC-1, that efficiently quenches fluorescence from a wide range of fluorophores spanning from 500 to 800 nm. High quality CuInS2/ZnS QDs with photoluminescence quantum yields (PLQYs) ~70% were prepared using ZnI2 as zinc source. To make them water-soluble and preserve small hydrodynamic size, amine-terminated lipoic acid will be used to cap CuInS2/ZnS QDs via ligand exchange. These water-soluble QDs can be easily combined with NHS ester group in IRDye QC-1. By tuning the PLQYs of donor and the degree of spectra overlap between the donor emission and the acceptor absorption, different Förster distance (R0) and efficiency of energy transfer (E) can be obtained. These optimized results will contribute to the further investigation on the Lipoprotein biointeractions via FRET.

[1] X. Peng, et al., Anal. Biochem. 388, 220–228(2009).

Gegenereerd op 2018-07-22 16:25:20
All publications
  2018 - Scholarly publications
Xia, Chenghui, Winckelmans, Naomi, Prins, P. Tim, Bals, Sara, Gerritsen, Hans & De Mello Donegá, Celso (23.03.2018). Near-Infrared-Emitting CuInS2/ZnS Dot-in-Rod Colloidal Heteronanorods by Seeded Growth. Journal of the American Chemical Society, 140 (17), (pp. 5755-5763) (9 p.).
  2017 - Scholarly publications
Xia, Chenghui, Meeldijk, Johannes D., Gerritsen, Hans C. & De Mello Donega, Celso (13.06.2017). Highly Luminescent Water-Dispersible NIR-Emitting Wurtzite CuInS2/ZnS Core/Shell Colloidal Quantum Dots. Chemistry of Materials, 29 (11), (pp. 4940-4951) (12 p.).
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Gegenereerd op 2018-07-22 16:25:20
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C. Xia Contact details
Leonard S. Ornsteinlaboratorium

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Gegenereerd op 2018-07-22 16:25:20
Last updated 09.07.2018