C. (Chenghui) Xia
C.Xia@uu.nl
Gegenereerd op 2017-10-18 05:56:10


Profile

Dark quenchers, namely nonfluorescent quenching dyes, have been commonly applied in Förster resonance energy transfer (FRET) based fluorogenic probes for protease activity detection, nucleic acid hybridization and real-time polymerase chain reaction. In FRET-based systems, a specific quencher is normally able to quench the fluorescence only from those fluorophore donors that have significant overlap of their emission spectra with the absorption spectrum of the quencher when the donor and quencher are brought into proximity [1]

In our work, we will fabricate a FRET system choosing CuInS2/ZnS QDs as donor while dark dyes as acceptor by using water-soluble quencher, IRDye QC-1, that efficiently quenches fluorescence from a wide range of fluorophores spanning from 500 to 800 nm. High quality CuInS2/ZnS QDs with photoluminescence quantum yields (PLQYs) ~70% were prepared using ZnI2 as zinc source. To make them water-soluble and preserve small hydrodynamic size, amine-terminated lipoic acid will be used to cap CuInS2/ZnS QDs via ligand exchange. These water-soluble QDs can be easily combined with NHS ester group in IRDye QC-1. By tuning the PLQYs of donor and the degree of spectra overlap between the donor emission and the acceptor absorption, different Förster distance (R0) and efficiency of energy transfer (E) can be obtained. These optimized results will contribute to the further investigation on the Lipoprotein biointeractions via FRET.

[1] X. Peng, et al., Anal. Biochem. 388, 220–228(2009).

Gegenereerd op 2017-10-18 05:56:10

Xia, C.; Meeldijk, J. D.; Gerritsen, H. C.; de Mello Donega, C., Highly Luminescent Water-Dispersible NIR-Emitting Wurtzite CuInS2/ZnS Core/Shell Colloidal Quantum Dots. Chem. Mater. 2017, 29, 4940-4951.

^ top
Gegenereerd op 2017-10-18 05:56:10
Full name
C. Xia Contact details
Leonard S. Ornsteinlaboratorium

Princetonplein 1
Room 119
3584 CC  UTRECHT
The Netherlands


Phone number (direct) +31 62 477 0093
E-mail
C.Xia@uu.nl
Availability
Mo Tue Wed Thu Fr
Morning
Afternoon
Gegenereerd op 2017-10-18 05:56:10
Last updated 03.10.2017