Dr. B. (Bart) Spee

Associate Professor
Inwendige Ziekten
+31 30 212 1800
b.spee@uu.nl

Highlighted publications

Nantasanti, S., Spee, B., Kruitwagen, H. S., Chen, C., Geijsen, N., Oosterhoff, L. A., van Wolferen, M. E., Pelaez, N., Fieten, H., Wubbolts, R. W., Grinwis, G. C., Chan, J., Huch, M., Vries, R. R. G., Clevers, H., de Bruin, A., Rothuizen, J., Penning, L. C., & Schotanus, B. A. (2015). Disease Modeling and Gene Therapy of Copper Storage Disease in Canine Hepatic Organoids. Stem Cell Reports [E], 5(5), 895-907. https://doi.org/10.1016/j.stemcr.2015.09.002
Boulter, L., Govaere, O., Bird, T. G., Radulescu, S., Ramachandran, P., Pellicoro, A., Ridgway, R., Seo, S. S., Spee, B., van Rooijen, N., Sansom, O. J., Iredale, J. P., Lowell, S., Roskams, T. A., & Forbes, S. J. (2012). Macrophage-derived Wnt opposes notch signaling to specify hepatic progenitor cell fate in chronic liver disease. Nature Medicine, 18(4), 572-579. https://doi.org/10.1038/nm.2667
Spee, B., Carpino, G., Schotanus, B. A., Katoonizadeh, A., Vander Borght, S., Gaudio, E., & Roskams, T. (2010). Characterisation of the liver progenitor cell niche in liver diseases: potential involvement of Wnt and Notch signalling. Gut, 59(2), 247-257.
https://dspace.library.uu.nl/bitstream/handle/1874/204549/Characterisation%2520of%2520the%2520liver%2520progenitor....pdf?sequence=1
Schneeberger, K., Spee, B., Costa, P., Sachs, N., Clevers, H., & Malda, J. (2017). Converging biofabrication and organoid technologies: the next frontier in hepatic and intestinal tissue engineering? Biofabrication, 9, [013001]. https://doi.org/10.1088/1758-5090/aa6121
Malagola, E., Teunissen, M., van der Laan, L. J. W., Verstegen, M., Schotanus, B. A., van Steenbeek, F. G., Penning, L. C., van Wolferen, M. E., Tryfonidou, M. A., & Spee, B. (2016). Characterization and comparison of canine multipotent stromal cells derived from liver and bone marrow. Stem Cells and Development, 35(2). https://doi.org/10.1089/scd.2015.0125

Publications

2022

Scholarly publications

Wang, Z., Faria, J., van der Laan, L. J. W., Penning, L. C., Masereeuw, R., & Spee, B. (2022). Human Cholangiocytes Form a Polarized and Functional Bile Duct on Hollow Fiber Membranes. Frontiers in Bioengineering and Biotechnology, 10, [868857]. https://doi.org/10.3389/fbioe.2022.868857
Krüger, M., Ruppelt, A., Kappler, B., Van Soest, E., Samsom, R. A., Grinwis, G. C. M., Geijsen, N., Helms, J. B., Stijnen, M., Kock, L. M., Rasponi, M., Kooistra, H. S., & Spee, B. (2022). Normothermic Ex Vivo Liver Platform Using Porcine Slaughterhouse Livers for Disease Modeling. Bioengineering (Basel, Switzerland), 9(9), 1-19. [471]. https://doi.org/10.3390/bioengineering9090471
de Bruijn, V. M. P., Wang, Z., Bakker, W., Zheng, W., Spee, B., & Bouwmeester, H. (2022). Hepatic bile acid synthesis and secretion: comparison of in vitro methods. Toxicology Letters, 365, 46-60. https://doi.org/10.1016/j.toxlet.2022.06.004
Willemse, J., van Tienderen, G., van Hengel, E., Schurink, I., van der Ven, D., Kan, Y., de Ruiter, P., Rosmark, O., Westergren-Thorsson G, G., Schneeberger, K., van der Eerden, B., Roest, H., Spee, B., van der Laan, L., de Jonge, J., & Verstegen, M. (2022). Hydrogels derived from decellularized liver tissue support the growth and differentiation of cholangiocyte organoids. Biomaterials, 284, 1-16. [121473]. https://doi.org/10.1016/j.biomaterials.2022.121473
Bernal, P. N., Bouwmeester, M., Madrid-Wolff, J., Falandt, M., Florczak, S., Rodriguez, N. G., Li, Y., Größbacher, G., Samsom, R-A., van Wolferen, M., van der Laan, L., Delrot, P., Loterie, D., Malda, J., Moser, C., Spee, B., & Levato, R. (2022). Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver-Like Metabolic Biofactories. Advanced Materials, 34(15), 1-16. [2110054]. https://doi.org/10.1002/adma.202110054

2021

Scholarly publications

Lehmann, V., Schene, I. F., Ardisasmita, A. I., Liv, N., Veenendaal, T., Klumperman, J., van der Doef, H. P. J., Verkade, H. J., Verstegen, M. M. A., van der Laan, L. J. W., Jans, J. J. M., Verhoeven-Duif, N. M., van Hasselt, P. M., Nieuwenhuis, E. E. S., Spee, B., & Fuchs, S. A. (2021). The potential and limitations of intrahepatic cholangiocyte organoids to study inborn errors of metabolism. Journal of Inherited Metabolic Disease, [15498]. https://doi.org/10.1002/jimd.12450
Bouwmeester, M. C., Bernal, P. N., Oosterhoff, L. A., van Wolferen, M., Lehmann, V., Vermaas, M., Buchholz, M-B., Peiffer, Q., Malda, J., van der Laan, L. J. W., Kramer, N. I., Schneeberger, K., Levato, R., & Spee, B. (2021). Bioprinting of Human Liver-derived Epithelial Organoids for Toxicity Studies. Macromolecular Bioscience, 21(12), 1-10. [2100327]. https://doi.org/10.1002/mabi.202100327
HPB Organoid Consortium (2021). Building consensus on definition and nomenclature of hepatic, pancreatic, and biliary organoids. Cell Stem Cell, 28(5), 816-832. https://doi.org/10.1016/j.stem.2021.04.005

2020

Scholarly publications

Verstegen, M. M. A., Roos, F. J. M., Burka, K., Gehart, H., Jager, M., de Wolf, M., Bijvelds, M. J. C., de Jonge, H. R., Ardisasmita, A. I., van Huizen, N. A., Roest, H. P., de Jonge, J., Koch, M., Pampaloni, F., Fuchs, S. A., Schene, I. F., Luider, T. M., van der Doef, H. P. J., Bodewes, F. A. J. A., ... van der Laan, L. J. W. (2020). Human extrahepatic and intrahepatic cholangiocyte organoids show region-specific differentiation potential and model cystic fibrosis-related bile duct disease. Scientific Reports, 10(1), [21900]. https://doi.org/10.1038/s41598-020-79082-8
Wang, Z., Faria, J., Penning, L., Masereeuw, R., & Spee, B. (2020). Tissue Engineered Bile Ducts for Disease Modeling and Therapy. Tissue engineering. Part C, Methods. https://doi.org/10.1089/ten.TEC.2020.0283
Schneeberger, K., Bouwmeester, M., Levato, R., Kramer, N., Malda, J., & Spee, B. (2020). BIOFABRICATION OF LIVER CONSTRUCTS FOR DRUG TOXICITY STUDIES. Drug Metabolism and Pharmacokinetics, 35(1), S8-S8. https://doi.org/10.1016/j.dmpk.2020.04.295
Kunst, R. F., Niemeijer, M., van der Laan, L. J. W., Spee, B., & van de Graaf, S. F. J. (2020). From Fatty Hepatocytes to Impaired Bile Flow: Matching Model Systems for Liver Biology and Disease. Biochemical Pharmacology, 180, [114173]. https://doi.org/10.1016/j.bcp.2020.114173
Ye, S., Boeter, J. W. B., Mihajlovic, M., van Steenbeek, F. G., van Wolferen, M. E., Oosterhoff, L. A., Marsee, A., Caiazzo, M., van der Laan, L. J. W., Penning, L. C., Vermonden, T., Spee, B., & Schneeberger, K. (2020). A Chemically Defined Hydrogel for Human Liver Organoid Culture. Advanced Functional Materials, [2000893]. https://doi.org/10.1002/adfm.202000893
Punt, A., Bouwmeester, H., Blaauboer, B. J., Coecke, S., Hakkert, B., Hendriks, D. F. G., Jennings, P., Kramer, N. I., Neuhoff, S., Masereeuw, R., Paini, A., Peijnenburg, A. A. C. M., Rooseboom, M., Shuler, M. L., Sorrell, I., Spee, B., Strikwold, M., Van der Meer, A. D., Van der Zande, M., ... Heringa, M. B. (2020). New approach methodologies (NAMs) for human-relevant biokinetics predictions: Meeting the paradigm shift in toxicology towards an animal-free chemical risk assessment. Altex. https://doi.org/10.14573/altex.2003242
Schene, I. F., Ramos, R. J., van Vugt, A. H. M., Truong, H., van den Heuvel, D. J., Gerritsen, H. C., Houtkooper, R. H., van Weeghel, M., Middendorp, S., Mokry, M., Spee, B., Schneeberger, K., Nikkels, P. G. J., Huch, M., Clevers, H., Nieuwenhuis, E. E. S., van Hasselt, P. M., & Fuchs, S. A. (2020). Bipotent Liver Progenitors Depend on Glycolysis and Mitochondrial Pyruvate Oxidation for Stem Cell Functions. SSRN Electronic Journal. https://doi.org/10.2139/ssrn.3564994
Li, Y., Qu, C., Spee, B., Zhang, R., Penning, L. C., de Man, R. A., Peppelenbosch, M. P., Fieten, H., & Pan, Q. (2020). Hepatitis E virus seroprevalence in pets in the Netherlands and the permissiveness of canine liver cells to the infection. Irish Veterinary Journal, 73(1), [6]. https://doi.org/10.1186/s13620-020-00158-y
Kramer, N., Pratscher, B., Meneses, A. M. C., Tschulenk, W., Walter, I., Swoboda, A., Kruitwagen, H. S., Schneeberger, K., Penning, L. C., Spee, B., Kieslinger, M., Brandt, S., & Burgener, I. A. (2020). Generation of Differentiating and Long-Living Intestinal Organoids Reflecting the Cellular Diversity of Canine Intestine. Cells, 9(4), [822]. https://doi.org/10.3390/cells9040822
Kruitwagen, H. S., Oosterhoff, L. A., van Wolferen, M. E., Chen, C., Nantasanti Assawarachan, S., Schneeberger, K., Kummeling, A., van Straten, G., Akkerdaas, I. C., Vinke, C. R., van Steenbeek, F. G., van Bruggen, L. W. L., Wolfswinkel, J., Grinwis, G. C. M., Fuchs, S. A., Gehart, H., Geijsen, N., Vries, R. G., Clevers, H., ... Spee, B. (2020). Long-Term Survival of Transplanted Autologous Canine Liver Organoids in a COMMD1-Deficient Dog Model of Metabolic Liver Disease. Cells, 9(2), 1-16. [410]. https://doi.org/10.3390/cells9020410
Krüger, M., Oosterhoff, L. A., van Wolferen, M. E., Schiele, S. A., Walther, A., Geijsen, N., De Laporte, L., van der Laan, L. J. W., Kock, L. M., & Spee, B. (2020). Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids. Advanced healthcare materials, 9(6), [1901658]. https://doi.org/10.1002/adhm.201901658
Haaker, M. W., Kruitwagen, H. S., Vaandrager, A. B., Houweling, M., Penning, L. C., Molenaar, M. R., van Wolferen, M. E., Oosterhoff, L. A., Spee, B., & Helms, J. B. (2020). Identification of potential drugs for treatment of hepatic lipidosis in cats using an in vitro feline liver organoid system. Journal of Veterinary Internal Medicine, 34(1), 132-138. https://doi.org/10.1111/jvim.15670

2019

Scholarly publications

Schneeberger, K., Sánchez-Romero, N., Ye, S., van Steenbeek, F. G., Oosterhoff, L. A., Pla Palacin, I., Chen, C., van Wolferen, M. E., van Tienderen, G., Lieshout, R., Colemonts-Vroninks, H., Schene, I., Hoekstra, R., Verstegen, M. M. A., van der Laan, L. J. W., Penning, L. C., Fuchs, S. A., Clevers, H., De Kock, J., ... Spee, B. (Accepted/In press). Large-scale Production of LGR5-positive Bipotential Human Liver Stem Cells. Hepatology. https://doi.org/10.1002/hep.31037
Krüger, M., Spee, B., Walther, A., De Laporte, L., & Kock, L. M. (2019). Nanofibrillar Cellulose as an Enzymatically and Flow Driven Degradable Scaffold for Three-Dimensional Tissue Engineering. Journal of Engineering and Science in Medical Diagnostics and Therapy, 2(4). https://doi.org/10.1115/1.4044473
Verstegen, M. M. A., Spee, B., & van der Laan, L. J. W. (2019). Bioengineering Liver Transplantation. Bioengineering (Basel, Switzerland), 6(4). https://doi.org/10.3390/bioengineering6040096
Ye, S., Boeter, J. W. B., Penning, L. C., Spee, B., & Schneeberger, K. (2019). Hydrogels for Liver Tissue Engineering. Bioengineering (Basel, Switzerland), 6(3), [59]. https://doi.org/10.3390/bioengineering6030059
Klotz, B. J., Oosterhoff, L. A., Utomo, L., Lim, K. S., Vallmajo-Martin, Q., Clevers, H., Woodfield, T. B. F., Rosenberg, A. J. W. P., Malda, J., Ehrbar, M., Spee, B., & Gawlitta, D. (2019). A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues. Advanced healthcare materials, 2019(8), [e1900979]. https://doi.org/10.1002/adhm.201900979
van Straten, G., van Dalen, D., Mesu, S. J., Rothuizen, J., Teske, E., Spee, B., Favier, R. P., & van Geijlswijk, I. M. (2019). Efficacy of orally administered sodium benzoate and sodium phenylbutyrate in dogs with congenital portosystemic shunts. Journal of Veterinary Internal Medicine, 33(3), 1331-1335. https://doi.org/10.1111/jvim.15477
Oosterhoff, L. A., Kruitwagen, H. S., van Wolferen, M. E., van Balkom, B. W. M., Mokry, M., Lansu, N., van den Dungen, N. A. M., Penning, L. C., Spanjersberg, T. C. F., de Graaf, J. W., Veenendaal, T., Zomerdijk, F., Fledderus, J. O., Spee, B., & van Steenbeek, F. G. (2019). Characterization of Endothelial and Smooth Muscle Cells From Different Canine Vessels. Frontiers in Physiology, 10, [101]. https://doi.org/10.3389/fphys.2019.00101
Sakai, M., Spee, B., Grinwis, G. C. M., Penning, L. C., van Wolferen, M. E., van der Laan, L. J. W., & Fieten, H. (2019). Association of circulating microRNA-122 and microRNA-29a with stage of fibrosis and progression of chronic hepatitis in Labrador Retrievers. Journal of Veterinary Internal Medicine, 33(1), 151-157. https://doi.org/10.1111/jvim.15366

2018

Scholarly publications

Kruitwagen, H. S., Westendorp, B., Viebahn, C. S., Post, K., van Wolferen, M. E., Oosterhoff, L. A., Egan, D. A., Delabar, J-M., Toussaint, M. J., Schotanus, B. A., de Bruin, A., Rothuizen, J., Penning, L. C., & Spee, B. (2018). DYRK1A Is a Regulator of S-Phase Entry in Hepatic Progenitor Cells. Stem Cells and Development, 27(2), 133-146. https://doi.org/10.1089/scd.2017.0139
Chen, C., Pla-Palacín, I., Baptista, P. M., Shang, P., Oosterhoff, L. A., van Wolferen, M. E., Penning, L. C., Geijsen, N., & Spee, B. (2018). Hepatocyte-like cells generated by direct reprogramming from murine somatic cells can repopulate decellularized livers. Biotechnology and Bioengineering, 115(11), 2807-2816. https://doi.org/10.1002/bit.26784
Chen, C., Jochems, P., Salz, L., Schneeberger, K., Penning, L. C., van de Graaf, S. F. J., Beuers, U., Clevers, H., Geijsen, N., Masereeuw, R., & Spee, B. (2018). Bioengineered bile ducts recapitulate key cholangiocyte functions. Biofabrication, 10(3), [034103]. https://doi.org/10.1088/1758-5090/aac8fd
Van den Bossche, L., van Steenbeek, F. G., Weber, M. F., Spee, B., Penning, L. C., van Sluijs, F. J., Zomerdijk, F., Groot Koerkamp, M. J. A., Rothuizen, J., Burgener, I. A., & Kummeling, A. (2018). Genome-wide based model predicting recovery from portosystemic shunting after liver shunt attenuation in dogs. Journal of Veterinary Internal Medicine, 32(4), 1343-1352. https://doi.org/10.1111/jvim.15140
van Rijn, J. M., Ardy, R. C., Kuloğlu, Z., Härter, B., van Haaften-Visser, D. Y., van der Doef, H. P. J., van Hoesel, M., Kansu, A., van Vugt, A. H. M., Ng, M., Kokke, F. T. M., Krolo, A., Başaran, M. K., Kaya, N. G., Ünlüsoy Aksu, A., Dalgıç, B., Ozcay, F., Baris, Z., Kain, R., ... Boztug, K. (2018). Intestinal failure and aberrant lipid metabolism in patients with DGAT1 deficiency. Gastroenterology, 155(1), 130-143. https://doi.org/10.1053/j.gastro.2018.03.040
Chen, C., Soto-Gutierrez, A., Baptista, P. M., & Spee, B. (2018). Biotechnology Challenges to In Vitro Maturation of Hepatic Stem Cells. Gastroenterology. https://doi.org/10.1053/j.gastro.2018.01.066

2017

Scholarly publications

Kruitwagen, H. S., Oosterhoff, L. A., Vernooij, I. G. W. H., Schrall, I. M., van Wolferen, M. E., Bannink, F., Roesch, C., van Uden, L., Molenaar, M. R., Helms, J. B., Grinwis, G. C. M., Verstegen, M. M. A., van der Laan, L. J. W., Huch, M., Geijsen, N., Vries, R. R. G., Clevers, H., Rothuizen, J., Schotanus, B. A., ... Spee, B. (2017). Long-Term Adult Feline Liver Organoid Cultures for Disease Modeling of Hepatic Steatosis. Stem Cell Reports, 8(4), 822-830. https://doi.org/10.1016/j.stemcr.2017.02.015
Tuohetahuntila, M., Molenaar, M. R., Spee, B., Brouwers, J. F., Wubbolts, R., Houweling, M., Yan, C., Du, H., VanderVen, B. C., Vaandrager, A. B., & Helms, J. B. (2017). Lysosome-mediated degradation of a distinct pool of lipid droplets during hepatic stellate cell activation. Journal of Biological Chemistry, 292(30), 12436-12448. https://doi.org/10.1074/jbc.M117.778472
van Sprundel, R. G. H. M., van den Ingh, T. S., Schotanus, B. A., van Wolferen, M. E., Penning, L. C., Rothuizen, J., & Spee, B. (2017). Cellular characteristics of keratin 19-positive canine hepatocellular tumours explain its aggressive behaviour. AERA Open, 4(1), [e000212]. https://doi.org/10.1136/vetreco-2016-000212
van Straten, G., de Sain - van der Velden, M. G. M., van Geijlswijk, I. M., Favier, R. P., Mesu, S. J., Holwerda-Loof, N. E., van der Ham, M., Fieten, H., Rothuizen, J., Spee, B., & Verhoeven - Duif, N. M. (2017). Saline is as effective as nitrogen scavengers for treatment of hyperammonemia. Scientific Reports, 7(1), [13112]. https://doi.org/10.1038/s41598-017-12686-9
Van den Bossche, L., Schoonenberg, V. A. C., Burgener, I. A., Penning, L. C., Schrall, I. M., Kruitwagen, H. S., van Wolferen, M. E., Grinwis, G. C. M., Kummeling, A., Rothuizen, J., van Velzen, J. F., Stathonikos, N., Molenaar, M. R., Helms, B. J., Brouwers, J. F. H. M., Spee, B., & van Steenbeek, F. G. (2017). Aberrant hepatic lipid storage and metabolism in canine portosystemic shunts. PLoS One, 12(10), [e0186491]. https://doi.org/10.1371/journal.pone.0186491
Dirksen, K., Burgener, I. A., Rothuizen, J., van den Ingh, T. S. G. A. M., Penning, L. C., Spee, B., & Fieten, H. (2017). Sensitivity and Specificity of Plasma ALT, ALP, and Bile Acids for Hepatitis in Labrador Retrievers. Journal of Veterinary Internal Medicine, 31, 1017–1027. https://doi.org/10.1111/jvim.14716
Dirksen, K., Spee, B., Penning, L. C., van den Ingh, T. S. G. A. M., Burgener, I. A., Watson, A. L., Groot Koerkamp, M., Rothuizen, J., van Steenbeek, F. G., & Fieten, H. (2017). Gene expression patterns in the progression of canine copper-associated chronic hepatitis. PLoS One, 12(5), e0176826. https://doi.org/10.1371/journal.pone.0176826
Schneeberger, K., Spee, B., Costa, P., Sachs, N., Clevers, H., & Malda, J. (2017). Converging biofabrication and organoid technologies: the next frontier in hepatic and intestinal tissue engineering? Biofabrication, 9, [013001]. https://doi.org/10.1088/1758-5090/aa6121

2016

Scholarly publications

Meneses, A. M. C., Schneeberger, K., Kruitwagen, H. S., Penning, L. C., van Steenbeek, F. G., Burgener, I. A., & Spee, B. (2016). Intestinal Organoids-Current and Future Applications. Veterinary Sciences, 3(4). https://doi.org/10.3390/vetsci3040031
Meneses, A., Schneeberger, K., Kruitwagen, H., Penning, L., van Steenbeek, F., Burgener, I., & Spee, B. (2016). Intestinal Organoids—Current and Future Applications. Veterinary Sciences, 3(4), 31. https://doi.org/10.3390/vetsci3040031
Dirksen, K., Roelen, Y. S., van Wolferen, M. E., Kruitwagen, H. S., Penning, L. C., Burgener, I. A., Spee, B., & Fieten, H. (2016). Erythrocyte copper chaperone for superoxide dismutase and superoxide dismutase as biomarkers for hepatic copper concentrations in Labrador retrievers. Veterinary Journal, 218, 1-6. https://doi.org/10.1016/j.tvjl.2016.10.007
Oosterhoff, L. A., Kruitwagen, H. S., Spee, B., & van Steenbeek, F. G. (2016). Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins. Journal of Visualized Experiments, (117), [e54786]. https://doi.org/10.3791/54786
Dirksen, K., Verzijl, T., Grinwis, G. C., Favier, R. P., Penning, L. C., Burgener, I. A., van der Laan, L. J., Fieten, H., & Spee, B. (2016). Use of Serum MicroRNAs as Biomarker for Hepatobiliary Diseases in Dogs. Journal of Veterinary Internal Medicine, 30(6), 1816-1823. https://doi.org/10.1111/jvim.14602
Matondo, R. B., Toussaint, M. J., Govaert, K. M., van Vuuren, L. D., Nantasanti, S., Nijkamp, M. W., Pandit, S. K., Tooten, P. C., Koster, M. H., Holleman, K., Schot, A., Gu, G., Spee, B., Roskams, T., Rinkes, I. B., Schotanus, B., Kranenburg, O., & de Bruin, A. (2016). Surgical resection and radiofrequency ablation initiate cancer in cytokeratin-19+- liver cells deficient for p53 and Rb. Oncotarget, 7(34), 54662-54675. https://doi.org/10.18632/oncotarget.9952
Dirksen, K., Verzijl, T., van den Ingh, T. S. G. A. M., Vernooij, J. C. M., van der Laan, L. J. W., Burgener, I. A., Spee, B., & Fieten, H. (2016). Hepatocyte-derived microRNAs as sensitive serum biomarkers of hepatocellular injury in Labrador retrievers. Veterinary Journal, 211, 75-81. https://doi.org/10.1016/j.tvjl.2016.01.010
Maidina, T., Molenaar, M. R., Spee, B., Brouwers, J. F., Houweling, M., Vaandrager, A. B., & Helms, J. B. (2016). ATGL and DGAT1 are involved in the turnover of newly synthesized triacylglycerols in hepatic stellate cells. Journal of Lipid Research, 57, 1162-1174. https://doi.org/10.1194/jlr.M066415
Fieten, H., Gill, Y., Martin, A. J., Concilli, M., Dirksen, K., van Steenbeek, F. G., Spee, B., van den Ingh, T. S. G. A. M., Martens, E. C. C. P., Festa, P., Chesi, G., van de Sluis, B., Houwen, R. H. J. H., Watson, A. L., Aulchenko, Y. S., Hodgkinson, V. L., Zhu, S., Petris, M. J., Polishchuk, R. S., ... Rothuizen, J. (2016). The Menkes and Wilson disease genes counteract in copper toxicosis in Labrador retrievers: a new canine model for copper-metabolism disorders. Disease Models & Mechanisms, 9, 25-38. https://doi.org/10.1242/dmm.020263
Malagola, E., Teunissen, M., van der Laan, L. J. W., Verstegen, M., Schotanus, B. A., van Steenbeek, F. G., Penning, L. C., van Wolferen, M. E., Tryfonidou, M. A., & Spee, B. (2016). Characterization and comparison of canine multipotent stromal cells derived from liver and bone marrow. Stem Cells and Development, 35(2). https://doi.org/10.1089/scd.2015.0125

2015

Scholarly publications

van Straten, G., Spee, B., Rothuizen, J., van Straten, M., & Favier, R. P. (2015). Diagnostic value of the rectal ammonia tolerance test, fasting plasma ammonia and fasting plasma bile acids for canine portosystemic shunting. Veterinary Journal, 204(3), 282-286. https://doi.org/10.1016/j.tvjl.2015.04.020
Nantasanti, S., Spee, B., Kruitwagen, H. S., Chen, C., Geijsen, N., Oosterhoff, L. A., van Wolferen, M. E., Pelaez, N., Fieten, H., Wubbolts, R. W., Grinwis, G. C., Chan, J., Huch, M., Vries, R. R. G., Clevers, H., de Bruin, A., Rothuizen, J., Penning, L. C., & Schotanus, B. A. (2015). Disease Modeling and Gene Therapy of Copper Storage Disease in Canine Hepatic Organoids. Stem Cell Reports [E], 5(5), 895-907. https://doi.org/10.1016/j.stemcr.2015.09.002
Tuohetahuntila, M., Spee, B., Kruitwagen, H. S., Wubbolts, R., Brouwers, J. F., van de Lest, C. H., Molenaar, M. R., Houweling, M., Helms, J. B., & Vaandrager, A. B. (2015). Role of long-chain acyl-CoA synthetase 4 in formation of polyunsaturated lipid species in hepatic stellate cells. Biochimica et Biophysica Acta, 1851(2), 220-30. https://doi.org/10.1016/j.bbalip.2014.12.003
Favier, R. P., Spee, B., Fieten, H., van den Ingh, T. S. G. A. M., Schotanus, B. A., Brinkhof, B., Rothuizen, J., & Penning, L. C. (2015). Aberrant expression of copper associated genes after copper accumulation in COMMD1-deficient dogs. Journal of Trace Elements in Medicine and Biology, 29, 347-53. https://doi.org/10.1016/j.jtemb.2014.06.007

2014

Scholarly publications

Govaere, O., Komuta, M., Berkers, J., Spee, B., Janssen, C., de Luca, F., Katoonizadeh, A., Wouters, J., van Kempen, L. C., Durnez, A., Verslype, C., De Kock, J., Rogiers, V., van Grunsven, L. A., Topal, B., Pirenne, J., Vankelecom, H., Nevens, F., van den Oord, J., ... Roskams, T. (2014). Keratin 19: a key role player in the invasion of human hepatocellular carcinomas. Gut, 63(4), 674-85. https://doi.org/10.1136/gutjnl-2012-304351
van Sprundel, R. G. H. M., van den Ingh, T. S. G. A. M., Guscetti, F., Kershaw, O., van Wolferen, M. E., Rothuizen, J., & Spee, B. (2014). Classification of primary hepatic tumours in the cat. Veterinary Journal, 202(2), 255-66. https://doi.org/10.1016/j.tvjl.2014.07.002
van der Laan, L. J. W., de Ruiter, P. E., van Gils, I. M., Fieten, H., Spee, B., Pan, Q., Rothuizen, J., & Penning, L. C. (2014). Canine hepacivirus and idiopathic hepatitis in dogs from a Dutch cohort. Journal of Viral Hepatitis, 21(12), 894-896. https://doi.org/10.1111/jvh.12268
Kruitwagen, H. S., Spee, B., Viebahn, C. S., Venema, H. B., Penning, L. C., Grinwis, G. C. M., Favier, R. P., van den Ingh, T. S. G. A. M., Rothuizen, J., & Schotanus, B. A. (2014). The canine hepatic progenitor cell niche: molecular characterisation in health and disease. Veterinary Journal, 201(3), 345–352. https://doi.org/10.1016/j.tvjl.2014.05.024
Schotanus, B. A., Kruitwagen, H. S., van den Ingh, T., van Wolferen, M. E., Rothuizen, J., Penning, L. C., & Spee, B. (2014). Enhanced Wnt/ß-catenin and Notch signalling in the activated canine hepatic progenitor cell niche. BMC Veterinary Research, 10, [10:309 ]. https://doi.org/10.1186/s12917-014-0309-1
Kruitwagen, H. S., Spee, B., & Schotanus, B. A. (2014). Hepatic progenitor cells in canine and feline medicine: potential for regenerative strategies. BMC Veterinary Research, 10. https://doi.org/10.1186/1746-6148-10-137
van Straten, G., van Steenbeek, F. G., Grinwis, G. C. M., Favier, R. P., Kummeling, A., Gils, I. H., Fieten, H., Groot Koerkamp, M. J. A., Holstege, F. C. P., Rothuizen, J., & Spee, B. (2014). Aberrant expression and distribution of enzymes of the urea cycle and other ammonia metabolizing pathways in dogs with congenital portosystemic shunts. PLoS One, 9(6), [e100077]. https://doi.org/10.1371/journal.pone.0100077

Other output

Schotanus, B. A., Nantasanti, S., Huch, M., Oosterhoff, L., Fieten, H., Kruitwagen, H. S., van Wolferen, M., Grinwis, G. C. M., Wubbolts, R., Favier, R. P., Penning, L. C., Vries, R. G. J., Clevers, H., Rothuizen, J., & Spee, B. (2014). Wnt AND NOTCH REGULATE PROLIFERATION, MIGRATION AND DIFFERENTIATION OF HUMAN HEPATIC PROGENITOR CELLS AND CANINE LIVER ORGANOIDS: Journal of Hepatology. Journal of Hepatology, 60(1, Suppl), S272. [P610]. https://doi.org/10.1016/S0168-8278(14)60772-8

2013

Scholarly publications

van Steenbeek, F. G., Spee, B., Penning, L. C., Kummeling, A., Gils, I. H., Grinwis, G. C. M., van Leenen, D., Holstege, F. C. P., Vos-Loohuis, M., Rothuizen, J., & Leegwater, P. A. J. (2013). Altered subcellular localization of heat shock protein 90 is associated with impaired expression of the aryl hydrocarbon receptor pathway in dogs. PLoS One, 8(3), e57973. https://doi.org/10.1371/journal.pone.0057973
van Steenbeek, F. G., van den Bossche, L., Grinwis, G. C. M., Kummeling, A., Gils, I. H., Koerkamp, M. G., van Leenen, D., Holstege, F. C., Penning, L. C., Rothuizen, J., Leegwater, P. A. J., & Spee, B. (2013). Aberrant gene expression in dogs with portosystemic shunts. PLoS One, 8(2), e57662. https://doi.org/10.1371/journal.pone.0057662
Sprundel, R. C. H. M., van den Ingh, T. S. G. A. M., Guscetti, F., Kershaw, O., Kanemoto, H., Gils, H. M., Rothuizen, J., Roskams, T., & Spee, B. (2013). Classification of primary hepatic tumours in the dog. Veterinary Journal, 197(3), 596-666. https://doi.org/10.1016/j.tvjl.2013.05.027
Schotanus, B. A., Penning, L. C., & Spee, B. (2013). Potential of regenerative medicine techniques in canine hepatology. Veterinary Quarterly, 33(4), 207-216. https://doi.org/10.1080/01652176.2013.875240
Rutteman, G. R., Erich, S. A., Mol, J. A., Spee, B., Grinwis, G. C. M., Fleckenstein, L., London, C. A., & Efferth, T. (2013). Safety and efficacy field study of artesunate for dogs with non-resectable tumours. Anticancer Research, 33(5), 1819-1827.
Kanemoto, H., Sakai, M., Sakamoto, Y., & Spee, B. (2013). American Cocker Spaniel Chronic Hepatitis in Japan. Journal of Veterinary Internal Medicine, 27(5), 1041-1048. https://doi.org/10.1111/jvim.12126

Other output

Roelen, Y. S., Dirksen, K., Fieten, H., & Spee, B. (2013). A Biomarker for Copper associated Hepatitis in Labrador Retrievers. Abstract from Voorjaarsdagen, Amsterdam & Utrecht.

2012

Scholarly publications

Andersen, J. B., Spee, B., Blechacz, B. R., Avital, I., Komuta, M., Barbour, A., Conner, E. A., Gillen, M. C., Roskams, T., Roberts, L. R., Factor, V. M., & Thorgeirsson, S. S. (2012). Genomic and genetic characterization of cholangiocarcinoma identifies therapeutic targets for tyrosine kinase inhibitors. Gastroenterology, 142(4), 1021-1031.e15. https://doi.org/10.1053/j.gastro.2011.12.005
Boulter, L., Govaere, O., Bird, T. G., Radulescu, S., Ramachandran, P., Pellicoro, A., Ridgway, R., Seo, S. S., Spee, B., van Rooijen, N., Sansom, O. J., Iredale, J. P., Lowell, S., Roskams, T. A., & Forbes, S. J. (2012). Macrophage-derived Wnt opposes notch signaling to specify hepatic progenitor cell fate in chronic liver disease. Nature Medicine, 18(4), 572-579. https://doi.org/10.1038/nm.2667
Favier, R. P., Spee, B., Schotanus, B. A., van den Ingh, T. S. G. A. M., Fieten, H., Brinkhof, B., Viebahn, C. S., Penning, L. C., & Rothuizen, J. (2012). COMMD1-Deficient Dogs Accumulate Copper in Hepatocytes and Provide a Good Model for Chronic Hepatitis and Fibrosis. PLoS One, 7(8), e42158. https://doi.org/10.1371/journal.pone.0042158

2011

Scholarly publications

Favier, R. P., Spee, B., Penning, L. C., & Rothuizen, J. (2011). Copper-induced hepatitis: the COMMD1 deficient dog as a translational animal model for human chronic hepatitis. Veterinary Quarterly, 31(1), 49-60. https://doi.org/10.1080/01652176.2011.563146
Kruitwagen, H. S., Arends, B., Spee, B., Brinkhof, B., van den Ingh, T. S. G. A. M., Rutten, V. P. M. G., Penning, L. C., Roskams, T., & Rothuizen, J. (2011). Recombinant hepatocyte growth factor treatment in a canine model of congenital liver hypoplasia. Liver International, 31(7), 940-949. https://doi.org/10.1111/j.1478-3231.2011.02513.x

2010

Scholarly publications

Spee, B., Carpino, G., Schotanus, B. A., Katoonizadeh, A., Vander Borght, S., Gaudio, E., & Roskams, T. (2010). Characterisation of the liver progenitor cell niche in liver diseases: potential involvement of Wnt and Notch signalling. Gut, 59(2), 247-257.
https://dspace.library.uu.nl/bitstream/handle/1874/204549/Characterisation%2520of%2520the%2520liver%2520progenitor....pdf?sequence=1
Sprundel, R. G., van den Ingh, T. S. G. A. M., Desmet, V. J., Katoonizadeh, A., Penning, L. C., Rothuizen, J., Roskams, T., & Spee, B. (2010). Keratin 19 marks poor differentiation and a more aggressive behaviour in canine and human hepatocellular tumours. Comparative Hepatology, 9, 4. http://www.comparative-hepatology.com/content/9/1/4

2009

Scholarly publications

Fieten, H., Spee, B., Ijzer, J., Kik, M. J., Penning, L. C., & Kirpensteijn, J. (2009). Expression of hepatocyte growth factor and the proto-oncogenic receptor c-Met in canine osteosarcoma. Veterinary Pathology, 46(5), 869-877. https://doi.org/10.1354/vp.08-VP-0155-F-FL
Arends, B., Spee, B., Schotanus, B. A., Roskams, T., van den Ingh, T. S. G. A. M., Penning, L. C., & Rothuizen, J. (2009). In vitro differentiation of liver progenitor cells derived from healthy dog livers. Stem Cells and Development, 18(2), 351-358.
Buijtels, J. J. C. W. M., de Gier, J., van Haeften, T., Kooistra, H. S., Spee, B., Veldhuis - Kroeze, E. J. B., Zijlstra, C., & Okkens, A. C. (2009). Minimal External Masculinization in a SRY-negative XX Male Podenco Dog. Reproduction in Domestic Animals, 44, 751-756.
Schotanus, B. A., van den Ingh, T. S. G. A. M., Penning, L. C., Rothuizen, J., Roskams, T. A., & Spee, B. (2009). Cross-species immunohistochemical investigation of the activation of the liver progenitor cell niche in different types of liver disease. Liver International, 28(9), 1241-1252.
Arends, B., Vankelecom, H., van der Borght, S., Roskams, T., Penning, L. C., Rothuizen, J., & Spee, B. (2009). The dog liver contains a "side population" of cells with hepatic progenitor-like characteristics. Stem Cells and Development, 18(2), 343-350. https://doi.org/10.1089/scd.2008.0022
Schlotter, Y. M., Veenhof, E. Z., Brinkhof, B., Rutten, V. P. M. G., Spee, B., Willemse, A., & Penning, L. C. (2009). A GeNorm algorithm-based selection of reference genes for quantitative real-time PCR in skin biopsies of healthy dogs and dogs with atopic dermatitis. Veterinary Immunology and Immunopathology, 129(1-2), 115-118.

2008

Scholarly publications

Arends, B., Spee, B., Hoffmann, G., Jansen, G. E. G., Slump, E., Auriemma, E., IJzer, J., Hemrika, W., Romijn, R. A. P., Van der Heijden-Liefkens, K. H. A., Sondermeijer, P. J. A., van den Ingh, T. S. G. A. M., Penning, L. C., & Rothuizen, J. (2008). In vitro and in vivo bioactivity of recombinant canine hepatocyte growth factor. Veterinary Journal, 178, 70-77.
https://dspace.library.uu.nl/bitstream/handle/1874/32230/2.pdf?sequence=1
Komuta, M., Spee, B., van der Borght, S., de Vos, R., Verslype, C., Aerts, R., Yano, H., Suzuki, T., Matsuda, M., Fujii, H., Desmet, V. J., Kojiro, M., & Roskams, T. (2008). Clinicopathological study on cholangiolocellular carcinoma suggesting hepatic progenitor cell origin. Hepatology, 47(5), 1544-1556.
Arends, B., Slump, E., Spee, B., Rothuizen, J., & Penning, L. C. (2008). Hepatocyte growth factor improves viability after H2O2-induced toxicity in bile duct epithelial cells. Comparative Biochemistry and Physiology A-Molecular and Integrative Physiology, 147(3), 324-330.

2007

Scholarly publications

Spee, B., Arends, B., Vankelecom, H., Rothuizen, J., Penning, L. C., & Roslams, T. A. (2007). Side-population analysis in healthy canine livers, a new source of liver specific progenitor cells? In Molecular Mechanisms of Regeneration / From Stem Cells to Organ Development, Function and Regeneration"
Spee, B., Arends, B., van den Ingh, T. S. G. A. M., Roskams, T., Rothuizen, J., & Penning, L. C. (2007). Major HGF-mediated regenerative pathways are similarly affected in human and canine cirrhosis. Comparative Hepatology, 6, 8. https://doi.org/10.1186/1476-5926-6-8
Spee, B., Arends, B., Wees, A. M., Bode, P., Penning, L. C., & Rothuizen, J. (2007). Functional consequences of RNA interference targeting COMMD1 in a canine hepatic cell line in relation to copper toxicosis. Animal Genetics, 38, 168-170.
https://dspace.library.uu.nl/bitstream/handle/1874/26165/Spee%2C%2520Arends%2C%2520Wees%2520van%2C%2520Bode%2C%2520Penning%2C%2520Rothuizen-Functional%2520consequences%2520of%2520RNA%2520interference%2520targeting%2520COMMD1%2520in%2520a%2520canine%2520hepatic%2520cell%2520line%2520in%2520relation%2520to%2520copper%2520toxicosis.pdf?sequence=1

2006

Scholarly publications

Spee, B., Arends, B., van den Ingh, T. S. G. A. M., Penning, L. C., & Rothuizen, J. (2006). Copper metabolism and oxidative stress in chronic inflammatory and cholestatic liver diseases in dogs. Journal of Veterinary Internal Medicine, 20, 1085-1092.
Spee, B., Jonkers, M. D., Arends, B., Rutteman, G. R., Rothuizen, J., & Penning, L. C. (2006). Specific down-regulation of XIAP with RNA interference enhances the sensitivity of canine tumor cell-lines to TRAIL and doxorubicin. Molecular Cancer, 5(34).
https://dspace.library.uu.nl/bitstream/handle/1874/20238/Spee%2C%2520Jonkers%2C%2520Arends%2C%2520Rutteman%2C%2520Rothuizen%2C%2520Penning%2520-%2520Specific%2520down-regulation%2520of%2520XIAP%2520with%2520RNA%2520interference%2520enhances%2520the%2520sensitivity%2520of%2520canine%2520tumor%2520cell-lines%2520to%2520TRAIL%2520and%2520doxorubicin.pdf?sequence=1
Brinkhof, B., Spee, B., Rothuizen, J., & Penning, L. C. (2006). Development and evaluation of canine reference genes for accurate quantification of gene expression. Analytical Biochemistry, 356, 36-43.
https://dspace.library.uu.nl/bitstream/handle/1874/20199/Brinkhof%2C%2520Spee%2C%2520Rothuizen%2C%2520Penning-Development%2520and%2520evaluation%2520of%2520canine%2520reference%2520genes%2520for%2520accurate%2520quantification%2520of%2520gene%2520expression.pdf?sequence=1
Spee, B. (2006). Regenerative and Fibrotic Pathways in Canine Liver Disease. [Doctoral thesis 1 (Research UU / Graduation UU), Utrecht University].
Spee, B., Arends, B., van den Ingh, T. S. G. A. M., Brinkhof, B., IJzer, J., Nederbragt, H., Penning, L. C., & Rothuizen, J. (2006). TGFB-1 signalling in canine hepatic diseases, new models for human fibrotic liver pathologies. Liver International, 26(6), 716-725.

2005

Scholarly publications

Spee, B., Penning, L. C., van den Ingh, T. S. G. A. M., Arends, B., IJzer, J., van Sluijs, F. J., & Rothuizen, J. (2005). Regenerative and fibrotic pathways in canine hepatic portosystemic shunt and portal vein hypoplasia, new models for clinical hepatocyte growth factor treatment. Comparative Hepatology, 4(7), 1-11.
Spee, B., Arends, B., van den Ingh, T. S. G. A. M., Brinkhof, B., Nederbragt, H., IJzer, J., Penning, L. C., & Rothuizen, J. (2005). Pathways of liver fibrosis and regeneration in the dog as a basis for new therapies. In ECVIM-CA Congress Proceedings (pp. 179-181)
Favier, R. P., Spee, B., Penning, L. C., Brinkhof, B., & Rothuizen, J. (2005). Quantitative PCR method to detect a 13-kb deletion in the MURR1 gene associated with copper toxicosis and HIV-1 replication. Mammalian Genome, 16, 460-463.
Penning, L. C., Spee, B., Rothuizen, J., & Rutteman, G. R. (2005). Use of regenerative pathways by tumors ? Expression of oncogenes, tumor suppressor genes and gene products involved in cell- cell interactions in canine liver diseases. In G. R. Rutteman, & L. C. Penning (Eds.), Tumor Cell Stromal Cell Interactions (pp. 5-6).
Spee, B., Mandigers, P. J. J., Arends, B., Bode, P., van den Ingh, T. S. G. A. M., Hoffmann, G., Rothuizen, J., & Penning, L. C. (2005). Differential expression of copper-associated and oxidative stress related proteins in a new variant of copper toxicosis in Doberman pinschers. Comparative Hepatology, 4(3), 1-13.

2004

Scholarly publications

Boomkens, S. Y., Spee, B., IJzer, J., Kisjes, R., Egberink, H. F., van den Ingh, T. S. G. A. M., Rothuizen, J., & Penning, L. C. (2004). The establishment and characterization of the first canine hepatocellular carcinoma cell line, which resembles human oncogenic expression patterns. Comparative Hepatology, 3, 9.
Schipper, L., Spee, B., Rothuizen, J., Woutersen-van Nijnanten, F., & Fink-Gremmels, J. (2004). Characterisation of 11 ß-hydroxysteroid dehydrogenases in feline kidney and liver. Biochimica et Biophysica Acta. Molecular Basis of Disease, 1688, 68-77.
Mandigers, P. J. J., van den Ingh, T. S. G. A. M., Spee, B., Penning, L. C., Bode, P., & Rothuizen, J. (2004). Chronic hepatitis in Doberman pinschers. A review. Veterinary Quarterly, 26(3), 99-106.
Mandigers, P. J. J., van den Ingh, T. S. G. A. M., Spee, B., Penning, L. C., & Rothuizen, J. (2004). The pathogenesis of Doberman hepatitis. In 14th Symposium ECVIM-CA (pp. 120-123)
Boomkens, S. Y., Kusters, J. G., Hoffmann, G., Pot, R. G., Spee, B., Penning, L. C., Egberink, H. F., van den Ingh, T. S. G. A. M., & Rothuizen, J. (2004). Detection of Helicobacter pylori in bile of cats. FEMS Immunology and Medical Microbiology, 42, 307-311. https://doi.org/10.1016/j.femsim.2004.06.002

2003

Scholarly publications

Bull, S., Laan, T. T. J. M., de Vrieze, G., Spee, B., van den Hoven, R., & Fink-Gremmels, J. (2003). Modulation of LPS-induved cytokine production by clenbuterol in equine alveolar macrophages. In Journal of veterinary pharmacology and therapeutics (pp. 234-235)