dr. B. (Bart) Spee
Assistant Professor

Gegenereerd op 2017-09-23 23:46:26


Gegenereerd op 2017-09-23 23:46:26
Key publications

Malagola, Ermanno, Teunissen, Michelle, van der Laan, Luc J W, Verstegen, Monique, Schotanus, Baukje Akke, van Steenbeek, Frank G, Penning, Louis C, van Wolferen, Monique E, Tryfonidou, Marianna A & Spee, Bart (2016). Characterization and comparison of canine multipotent stromal cells derived from liver and bone marrow. Stem Cells and Development, 35 (2).

Nantasanti, Sathidpak, Spee, Bart, Kruitwagen, Hedwig S, Chen, Chen, Geijsen, Niels, Oosterhoff, Loes A, van Wolferen, Monique E, Pelaez, Nicolas, Fieten, Hille, Wubbolts, Richard W, Grinwis, Guy C, Chan, Jefferson, Huch, Meritxell, Vries, Robert R G, Clevers, Hans, de Bruin, Alain, Rothuizen, Jan, Penning, Louis C & Schotanus, Baukje A (2015). Disease Modeling and Gene Therapy of Copper Storage Disease in Canine Hepatic Organoids. Stem Cell Reports, 5 (5), (pp. 895-907) (13 p.).

Boulter, L., Govaere, O., Bird, T.G., Radulescu, S., Ramachandran, P., Pellicoro, A., Ridgway, R., Seo, S.S., Spee, B., van Rooijen, N., Sansom, O.J., Iredale, J.P., Lowell, S., Roskams, T.A. & Forbes, S.J. (2012). Macrophage-derived Wnt opposes notch signaling to specify hepatic progenitor cell fate in chronic liver disease. Nature Medicine, 18 (4), (pp. 572-579) (8 p.).

Spee, B., Carpino, G., Schotanus, B.A., Katoonizadeh, A., Vander Borght, S., Gaudio, E. & Roskams, T. (2010). Characterisation of the liver progenitor cell niche in liver diseases: potential involvement of Wnt and Notch signalling. Gut, 59 (2), (pp. 247-257) (11 p.).

All publications
  Articles - 2017
Schneeberger, Kerstin, Spee, Bart, Costa, Pedro, Sachs, Norman, Clevers, Hans & Malda, Jos (17.02.2017). Converging biofabrication and organoid technologies - the next frontier in hepatic and intestinal tissue engineering?. Biofabrication Copyright 2017 IOP Publishing Ltd..
Dirksen, Karen, Spee, Bart, Penning, Louis C, van den Ingh, Ted S G A M, Burgener, Iwan A, Watson, Adrian L, Groot Koerkamp, Marian, Rothuizen, Jan, van Steenbeek, Frank G & Fieten, Hille (2017). Gene expression patterns in the progression of canine copper-associated chronic hepatitis. PLoS One, 12 (5), (pp. e0176826).
Kruitwagen, Hedwig S, Oosterhoff, Loes A, Vernooij, Ingrid G W H, Schrall, Ingrid M, van Wolferen, Monique E, Bannink, Farah, Roesch, Camille, van Uden, Lisa, Molenaar, Martijn R, Helms, J Bernd, Grinwis, Guy C M, Verstegen, Monique M A, van der Laan, Luc J W, Huch, Meritxell, Geijsen, Niels, Vries, Robert G, Clevers, Hans, Rothuizen, Jan, Schotanus, Baukje A, Penning, Louis C & Spee, Bart (2017). Long-Term Adult Feline Liver Organoid Cultures for Disease Modeling of Hepatic Steatosis. Stem Cell Reports Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved..
Tuohetahuntila, Maidina, Molenaar, Martijn R, Spee, Bart, Brouwers, Jos F, Wubbolts, Richard, Houweling, Martin, Yan, Cong, Du, Hong, VanderVen, Brian C, Vaandrager, Arie B & Helms, J Bernd (14.06.2017). Lysosome-mediated degradation of a distinct pool of lipid droplets during hepatic stellate cell activation. Journal of Biological Chemistry Copyright © 2017, The American Society for Biochemistry and Molecular Biology..
Dirksen, K, Burgener, I A, Rothuizen, J, van den Ingh, T S G A M, Penning, L C, Spee, B & Fieten, H (2017). Sensitivity and Specificity of Plasma ALT, ALP, and Bile Acids for Hepatitis in Labrador Retrievers. Journal of Veterinary Internal Medicine Copyright © 2017 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine..
  Articles - 2016
Maidina, Tuohetahuntila, Molenaar, Martijn R, Spee, Bart, Brouwers, Jos F, Houweling, Martin, Vaandrager, Arie B & Helms, J Bernd (14.05.2016). ATGL and DGAT1 are involved in the turnover of newly synthesized triacylglycerols in hepatic stellate cells. Journal of Lipid Research, 57, (pp. 1162-1174). Copyright © 2016, The American Society for Biochemistry and Molecular Biology..
Malagola, Ermanno, Teunissen, Michelle, van der Laan, Luc J W, Verstegen, Monique, Schotanus, Baukje Akke, van Steenbeek, Frank G, Penning, Louis C, van Wolferen, Monique E, Tryfonidou, Marianna A & Spee, Bart (2016). Characterization and comparison of canine multipotent stromal cells derived from liver and bone marrow. Stem Cells and Development, 35 (2).
Dirksen, K, Roelen, Y S, van Wolferen, M E, Kruitwagen, H S, Penning, L C, Burgener, I A, Spee, B & Fieten, H (2016). Erythrocyte copper chaperone for superoxide dismutase and superoxide dismutase as biomarkers for hepatic copper concentrations in Labrador retrievers. Veterinary Journal, 218, (pp. 1-6) (6 p.). Copyright © 2016 Elsevier Ltd. All rights reserved..
Dirksen, K, Verzijl, T, van den Ingh, T S G A M, Vernooij, J C M, van der Laan, L J W, Burgener, I A, Spee, B & Fieten, H (2016). Hepatocyte-derived microRNAs as sensitive serum biomarkers of hepatocellular injury in Labrador retrievers. Veterinary Journal, 211, (pp. 75-81) (7 p.). Copyright © 2016 Elsevier Ltd. All rights reserved..
Meneses, Andre, Schneeberger, Kerstin, Kruitwagen, Hedwig, Penning, Louis, van Steenbeek, Frank, Burgener, Iwan & Spee, Bart (21.10.2016). Intestinal Organoids—Current and Future Applications. Veterinary Sciences, 3 (4), (pp. 31) (1 p.).
Oosterhoff, Loes A, Kruitwagen, Hedwig S, Spee, Bart & van Steenbeek, Frank G (18.11.2016). Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins. Journal of Visualized Experiments (117).
Matondo, Ramadhan B, Toussaint, Mathilda Jm, Govaert, Klaas M, van Vuuren, Luciel D, Nantasanti, Sathidpak, Nijkamp, Maarten W, Pandit, Shusil K, Tooten, Peter Cj, Koster, Mirjam H, Holleman, Kaylee, Schot, Arend, Gu, Guoqiang, Spee, Bart, Roskams, Tania, Rinkes, Inne Borel, Schotanus, Baukje, Kranenburg, Onno & de Bruin, Alain (13.06.2016). Surgical resection and radiofrequency ablation initiate cancer in cytokeratin-19+- liver cells deficient for p53 and Rb. Oncotarget, 7 (34), (pp. 54662-54675).
Fieten, Hille, Gill, Yadvinder, Martin, Alan J, Concilli, Mafalda, Dirksen, Karen, van Steenbeek, Frank G, Spee, Bart, van den Ingh, Ted S G A M, Martens, Ellen C C P, Festa, Paola, Chesi, Giancarlo, van de Sluis, Bart, Houwen, Roderick H J H, Watson, Adrian L, Aulchenko, Yurii S, Hodgkinson, Victoria L, Zhu, Sha, Petris, Michael J, Polishchuk, Roman S, Leegwater, Peter A J & Rothuizen, Jan (01.01.2016). The Menkes and Wilson disease genes counteract in copper toxicosis in Labrador retrievers - a new canine model for copper-metabolism disorders. DMM Disease Models and Mechanisms, 9, (pp. 25-38) (14 p.). © 2016. Published by The Company of Biologists Ltd..
Dirksen, K, Verzijl, T, Grinwis, G C, Favier, R P, Penning, L C, Burgener, I A, van der Laan, L J, Fieten, H & Spee, B (2016). Use of Serum MicroRNAs as Biomarker for Hepatobiliary Diseases in Dogs. Journal of Veterinary Internal Medicine, 30 (6), (pp. 1816-1823) (8 p.). Copyright © 2016 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine..
  Articles - 2015
Favier, Robert P, Spee, Bart, Fieten, Hille, van den Ingh, Ted S G A M, Schotanus, Baukje A, Brinkhof, Bas, Rothuizen, Jan & Penning, Louis C (2015). Aberrant expression of copper associated genes after copper accumulation in COMMD1-deficient dogs. Journal of Trace Elements in Medicine and Biology, 29, (pp. 347-53) (7 p.). Copyright © 2014 Elsevier GmbH. All rights reserved..
van Straten, G, Spee, B, Rothuizen, J, van Straten, M & Favier, R P (2015). Diagnostic value of the rectal ammonia tolerance test, fasting plasma ammonia and fasting plasma bile acids for canine portosystemic shunting. Veterinary Journal, 204 (3), (pp. 282-286) (5 p.). Copyright © 2015 Elsevier Ltd. All rights reserved..
Nantasanti, Sathidpak, Spee, Bart, Kruitwagen, Hedwig S, Chen, Chen, Geijsen, Niels, Oosterhoff, Loes A, van Wolferen, Monique E, Pelaez, Nicolas, Fieten, Hille, Wubbolts, Richard W, Grinwis, Guy C, Chan, Jefferson, Huch, Meritxell, Vries, Robert R G, Clevers, Hans, de Bruin, Alain, Rothuizen, Jan, Penning, Louis C & Schotanus, Baukje A (2015). Disease Modeling and Gene Therapy of Copper Storage Disease in Canine Hepatic Organoids. Stem Cell Reports, 5 (5), (pp. 895-907) (13 p.).
Tuohetahuntila, Maidina, Spee, Bart, Kruitwagen, Hedwig S, Wubbolts, Richard, Brouwers, Jos F, van de Lest, Chris H, Molenaar, Martijn R, Houweling, Martin, Helms, J Bernd & Vaandrager, Arie B (2015). Role of long-chain acyl-CoA synthetase 4 in formation of polyunsaturated lipid species in hepatic stellate cells. Biochimica et Biophysica Acta, 1851 (2), (pp. 220-30) (11 p.). Copyright © 2014 Elsevier B.V. All rights reserved..
  Articles - 2014
van Straten, G., van Steenbeek, F.G., Grinwis, G.C.M., Favier, R.P., Kummeling, A., Gils, I.H., Fieten, H., Groot Koerkamp, M.J.A., Holstege, F.C.P., Rothuizen, J. & Spee, B. (2014). Aberrant expression and distribution of enzymes of the urea cycle and other ammonia metabolizing pathways in dogs with congenital portosystemic shunts. PLoS One, 9 (6) (11 p.).
van der Laan, L J W, de Ruiter, P E, van Gils, I M, Fieten, H, Spee, B, Pan, Q, Rothuizen, J & Penning, L C (2014). Canine hepacivirus and idiopathic hepatitis in dogs from a Dutch cohort. Journal of Viral Hepatitis, 21 (12), (pp. 894-896) (3 p.). © 2014 John Wiley & Sons Ltd..
van Sprundel, Renee G H M, van den Ingh, Ted S G A M, Guscetti, Franco, Kershaw, Olivia, van Wolferen, Monique E, Rothuizen, Jan & Spee, Bart (2014). Classification of primary hepatic tumours in the cat. Veterinary Journal, 202 (2), (pp. 255-66) (12 p.).
Schotanus, Baukje A, Kruitwagen, Hedwig S, van den Ingh, Ted, van Wolferen, Monique E, Rothuizen, Jan, Penning, Louis C & Spee, Bart (31.12.2014). Enhanced Wnt/ß-catenin and Notch signalling in the activated canine hepatic progenitor cell niche. BMC Veterinary Research, 10 (9 p.).
Kruitwagen, H.S., Spee, B. & Schotanus, B.A. (2014). Hepatic progenitor cells in canine and feline medicine: potential for regenerative strategies. BMC Veterinary Research, 10 (15 p.).
Govaere, Olivier, Komuta, Mina, Berkers, Johannes, Spee, Bart, Janssen, Carl, de Luca, Francesca, Katoonizadeh, Aezam, Wouters, Jasper, van Kempen, Léon C, Durnez, Anne, Verslype, Chris, De Kock, Joery, Rogiers, Vera, van Grunsven, Leo A, Topal, Baki, Pirenne, Jacques, Vankelecom, Hugo, Nevens, Frederik, van den Oord, Joost, Pinzani, Massimo & Roskams, Tania (2014). Keratin 19 - a key role player in the invasion of human hepatocellular carcinomas. Gut, 63 (4), (pp. 674-85) (12 p.).
Kruitwagen, H S, Spee, B, Viebahn, C S, Venema, H B, Penning, L C, Grinwis, G C M, Favier, R P, van den Ingh, T S G A M, Rothuizen, J & Schotanus, B A (2014). The canine hepatic progenitor cell niche - molecular characterisation in health and disease. Veterinary Journal, 201 (3), (pp. 345–352) (8 p.). Copyright © 2014 Elsevier Ltd. All rights reserved..
  Articles - 2013
van Steenbeek, F.G., van den Bossche, L., Grinwis, G.C.M., Kummeling, A., Gils, I.H., Koerkamp, M.G., van Leenen, D., Holstege, F.C., Penning, L.C., Rothuizen, J., Leegwater, P.A.J. & Spee, B. (2013). Aberrant gene expression in dogs with portosystemic shunts. PLoS One, 8 (2), (pp. e57662) (1 p.).
van Steenbeek, F.G., Spee, B., Penning, L.C., Kummeling, A., Gils, I.H., Grinwis, G.C.M., van Leenen, D., Holstege, F.C.P., Vos-Loohuis, M., Rothuizen, J. & Leegwater, P.A.J. (2013). Altered subcellular localization of heat shock protein 90 is associated with impaired expression of the aryl hydrocarbon receptor pathway in dogs. PLoS One, 8 (3), (pp. e57973) (1 p.).
Kanemoto, H., Sakai, M., Sakamoto, Y. & Spee, B. (2013). American Cocker Spaniel Chronic Hepatitis in Japan. Journal of Veterinary Internal Medicine, 27 (5), (pp. 1041-1048) (8 p.).
Sprundel, R.C.H.M., van den Ingh, T.S.G.A.M., Guscetti, F., Kershaw, O., Kanemoto, H., Gils, H.M., Rothuizen, J., Roskams, T. & Spee, B. (2013). Classification of primary hepatic tumours in the dog. Veterinary Journal, 197 (3), (pp. 596-666) (71 p.).
Schotanus, B.A., Penning, L.C. & Spee, B. (2013). Potential of regenerative medicine techniques in canine hepatology. Veterinary Quarterly, 33 (4), (pp. 207-216) (10 p.).
Rutteman, G.R., Erich, S.A., Mol, J.A., Spee, B., Grinwis, G.C.M., Fleckenstein, L., London, C.A. & Efferth, T. (2013). Safety and efficacy field study of artesunate for dogs with non-resectable tumours. Anticancer Research, 33 (5), (pp. 1819-1827) (9 p.).
  Articles - 2012
Favier, Robert P, Spee, Bart, Schotanus, Baukje A, van den Ingh, Ted S G A M, Fieten, Hille, Brinkhof, Bas, Viebahn, Cornelia S, Penning, Louis C & Rothuizen, Jan (2012). COMMD1-Deficient Dogs Accumulate Copper in Hepatocytes and Provide a Good Model for Chronic Hepatitis and Fibrosis. PLoS One, 7 (8), (pp. e42158).
Andersen, Jesper B, Spee, Bart, Blechacz, Boris R, Avital, Itzhak, Komuta, Mina, Barbour, Andrew, Conner, Elizabeth A, Gillen, Matthew C, Roskams, Tania, Roberts, Lewis R, Factor, Valentina M & Thorgeirsson, Snorri S (2012). Genomic and genetic characterization of cholangiocarcinoma identifies therapeutic targets for tyrosine kinase inhibitors. Gastroenterology, 142 (4), (pp. 1021-1031.e15). Copyright © 2012 AGA Institute. Published by Elsevier Inc. All rights reserved..
Boulter, L., Govaere, O., Bird, T.G., Radulescu, S., Ramachandran, P., Pellicoro, A., Ridgway, R., Seo, S.S., Spee, B., van Rooijen, N., Sansom, O.J., Iredale, J.P., Lowell, S., Roskams, T.A. & Forbes, S.J. (2012). Macrophage-derived Wnt opposes notch signaling to specify hepatic progenitor cell fate in chronic liver disease. Nature Medicine, 18 (4), (pp. 572-579) (8 p.).
  Articles - 2011
Favier, R.P., Spee, B., Penning, L.C. & Rothuizen, J. (2011). Copper-induced hepatitis: the COMMD1 deficient dog as a translational animal model for human chronic hepatitis. Veterinary Quarterly, 31 (1), (pp. 49-60) (12 p.).
Kruitwagen, H.S., Arends, B., Spee, B., Brinkhof, B., van den Ingh, T.S.G.A.M., Rutten, V.P.M.G., Penning, L.C., Roskams, T. & Rothuizen, J. (2011). Recombinant hepatocyte growth factor treatment in a canine model of congenital liver hypoplasia. Liver International, 31 (7), (pp. 940-949) (10 p.).
  Articles - 2010
Spee, B., Carpino, G., Schotanus, B.A., Katoonizadeh, A., Vander Borght, S., Gaudio, E. & Roskams, T. (2010). Characterisation of the liver progenitor cell niche in liver diseases: potential involvement of Wnt and Notch signalling. Gut, 59 (2), (pp. 247-257) (11 p.).
Sprundel, R.G., van den Ingh, T.S.G.A.M., Desmet, V.J., Katoonizadeh, A., Penning, L.C., Rothuizen, J., Roskams, T. & Spee, B. (2010). Keratin 19 marks poor differentiation and a more aggressive behaviour in canine and human hepatocellular tumours. Comparative Hepatology, 9, (pp. 4) (11 p.).
  Articles - 2009
Schlotter, Y.M., Veenhof, E.Z., Brinkhof, B., Rutten, V.P.M.G., Spee, B., Willemse, A. & Penning, L.C. (2009). A GeNorm algorithm-based selection of reference genes for quantitative real-time PCR in skin biopsies of healthy dogs and dogs with atopic dermatitis. Veterinary Immunology and Immunopathology, 129 (1-2), (pp. 115-118) (4 p.).
Schotanus, B.A., van den Ingh, T.S.G.A.M., Penning, L.C., Rothuizen, J., Roskams, T.A. & Spee, B. (2009). Cross-species immunohistochemical investigation of the activation of the liver progenitor cell niche in different types of liver disease. Liver International, 28 (9), (pp. 1241-1252) (12 p.).
Fieten, H, Spee, B, Ijzer, J, Kik, M J, Penning, L C & Kirpensteijn, J (2009). Expression of hepatocyte growth factor and the proto-oncogenic receptor c-Met in canine osteosarcoma. Veterinary Pathology, 46 (5), (pp. 869-877) (9 p.).
Arends, B., Spee, B., Schotanus, B.A., Roskams, T., van den Ingh, T.S.G.A.M., Penning, L.C. & Rothuizen, J. (2009). In vitro differentiation of liver progenitor cells derived from healthy dog livers. Stem Cells and Development, 18 (2), (pp. 351-358) (8 p.).
Buijtels, J.J.C.W.M., de Gier, J., van Haeften, T., Kooistra, H.S., Spee, B., Veldhuis - Kroeze, E.J.B., Zijlstra, C. & Okkens, A.C. (2009). Minimal External Masculinization in a SRY-negative XX Male Podenco Dog. Reproduction in Domestic Animals, 44, (pp. 751-756) (5 p.).
Arends, B., Vankelecom, H., van der Borght, S., Roskams, T., Penning, L.C., Rothuizen, J. & Spee, B. (2009). The dog liver contains a "side population" of cells with hepatic progenitor-like characteristics. Stem Cells and Development, 18 (2), (pp. 343-350) (8 p.).
  Articles - 2008
Komuta, M., Spee, B., van der Borght, S., de Vos, R., Verslype, C., Aerts, R., Yano, H., Suzuki, T., Matsuda, M., Fujii, H., Desmet, V.J., Kojiro, M. & Roskams, T. (2008). Clinicopathological study on cholangiolocellular carcinoma suggesting hepatic progenitor cell origin. Hepatology, 47 (5), (pp. 1544-1556) (13 p.).
Arends, B., Slump, E., Spee, B., Rothuizen, J. & Penning, L.C. (2008). Hepatocyte growth factor improves viability after H2O2-induced toxicity in bile duct epithelial cells. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 147 (3), (pp. 324-330) (7 p.).
Arends, B., Spee, B., Hoffmann, G., Jansen, G.E.G., Slump, E., Auriemma, E., IJzer, J., Hemrika, W., Romijn, R.A.P., Van der Heijden-Liefkens, K.H.A., Sondermeijer, P.J.A., van den Ingh, T.S.G.A.M., Penning, L.C. & Rothuizen, J. (2008). In vitro and in vivo bioactivity of recombinant canine hepatocyte growth factor. Veterinary Journal, 178, (pp. 70-77) (8 p.).
  Articles - 2007
Spee, B., Arends, B., Wees, A.M., Bode, P., Penning, L.C. & Rothuizen, J. (2007). Functional consequences of RNA interference targeting COMMD1 in a canine hepatic cell line in relation to copper toxicosis. Animal Genetics, 38, (pp. 168-170) (3 p.).
Spee, Bart, Arends, Brigitte, van den Ingh, Ted S G A M, Roskams, Tania, Rothuizen, Jan & Penning, Louis C (2007). Major HGF-mediated regenerative pathways are similarly affected in human and canine cirrhosis. Comparative Hepatology, 6, (pp. 8) (1 p.).
  Articles - 2006
Spee, B., Arends, B., van den Ingh, T.S.G.A.M., Penning, L.C. & Rothuizen, J. (2006). Copper metabolism and oxidative stress in chronic inflammatory and cholestatic liver diseases in dogs. Journal of Veterinary Internal Medicine, 20, (pp. 1085-1092) (8 p.).
Brinkhof, B., Spee, B., Rothuizen, J. & Penning, L.C. (2006). Development and evaluation of canine reference genes for accurate quantification of gene expression. Analytical Biochemistry, 356, (pp. 36-43) (8 p.).
Spee, B., Jonkers, M.D., Arends, B., Rutteman, G.R., Rothuizen, J. & Penning, L.C. (2006). Specific down-regulation of XIAP with RNA interference enhances the sensitivity of canine tumor cell-lines to TRAIL and doxorubicin. Molecular Cancer, 5 (34) (10 p.).
Spee, B., Arends, B., van den Ingh, T.S.G.A.M., Brinkhof, B., IJzer, J., Nederbragt, H., Penning, L.C. & Rothuizen, J. (2006). TGFB-1 signalling in canine hepatic diseases, new models for human fibrotic liver pathologies. Liver International, 26 (6), (pp. 716-725) (10 p.).
  Articles - 2005
Spee, B., Mandigers, P.J.J., Arends, B., Bode, P., van den Ingh, T.S.G.A.M., Hoffmann, G., Rothuizen, J. & Penning, L.C. (2005). Differential expression of copper-associated and oxidative stress related proteins in a new variant of copper toxicosis in Doberman pinschers. Comparative Hepatology, 4 (3), (pp. 1-13) (14 p.).
Favier, R.P., Spee, B., Penning, L.C., Brinkhof, B. & Rothuizen, J. (2005). Quantitative PCR method to detect a 13-kb deletion in the MURR1 gene associated with copper toxicosis and HIV-1 replication. Mammalian Genome, 16, (pp. 460-463) (4 p.).
Spee, B., Penning, L.C., van den Ingh, T.S.G.A.M., Arends, B., IJzer, J., van Sluijs, F.J. & Rothuizen, J. (2005). Regenerative and fibrotic pathways in canine hepatic portosystemic shunt and portal vein hypoplasia, new models for clinical hepatocyte growth factor treatment. Comparative Hepatology, 4 (7), (pp. 1-11) (11 p.).
  Articles - 2004
Schipper, L., Spee, B., Rothuizen, J., Woutersen-van Nijnanten, F. & Fink-Gremmels, J. (2004). Characterisation of 11 ß-hydroxysteroid dehydrogenases in feline kidney and liver. Biochimica et Biophysica Acta - Molecular Basis of Disease, 1688, (pp. 68-77) (10 p.).
Mandigers, P.J.J., van den Ingh, T.S.G.A.M., Spee, B., Penning, L.C., Bode, P. & Rothuizen, J. (2004). Chronic hepatitis in Doberman pinschers. A review. Veterinary Quarterly, 26 (3), (pp. 99-106) (8 p.).
Boomkens, S.Y., Kusters, J.G., Hoffmann, G., Pot, R.G., Spee, B., Penning, L.C., Egberink, H.F., van den Ingh, T.S.G.A.M. & Rothuizen, J. (2004). Detection of Helicobacter pylori in bile of cats. FEMS Immunology and Medical Microbiology, 42, (pp. 307-311) (5 p.).
Boomkens, S.Y., Spee, B., IJzer, J., Kisjes, R., Egberink, H.F., van den Ingh, T.S.G.A.M., Rothuizen, J. & Penning, L.C. (2004). The establishment and characterization of the first canine hepatocellular carcinoma cell line, which resembles human oncogenic expression patterns. Comparative Hepatology, 3, (pp. 9) (1 p.).
  Articles in volumes / proceedings - 2007
Spee, B., Arends, B., Vankelecom, H., Rothuizen, J., Penning, L.C. & Roslams, T.A. (31.08.2007). Side-population analysis in healthy canine livers, a new source of liver specific progenitor cells?. Molecular Mechanisms of Regeneration / From Stem Cells to Organ Development, Function and Regeneration" FEBS Spetses International Summer School 2007.
  Articles in volumes / proceedings - 2005
Spee, B., Arends, B., van den Ingh, T.S.G.A.M., Brinkhof, B., Nederbragt, H., IJzer, J., Penning, L.C. & Rothuizen, J. (01.09.2005). Pathways of liver fibrosis and regeneration in the dog as a basis for new therapies. ECVIM-CA Congress Proceedings (pp. 179-181) (3 p.). 15th ECVIM-CA Congress, Glasgow, Scotland.
Penning, L.C., Spee, B., Rothuizen, J. & Rutteman, G.R. (2005). Use of regenerative pathways by tumors ? Expression of oncogenes, tumor suppressor genes and gene products involved in cell- cell interactions in canine liver diseases. In G.R. Rutteman & L.C. Penning (Eds.), Tumor Cell Stromal Cell Interactions (pp. 5-6) (2 p.). Amsterdam, ESVONC precongress day of FECAVA/FEEVA/Voorjaarsdagen Congress april 14th 2005.
  Articles in volumes / proceedings - 2004
Mandigers, P.J.J., van den Ingh, T.S.G.A.M., Spee, B., Penning, L.C. & Rothuizen, J. (2004). The pathogenesis of Doberman hepatitis. 14th Symposium ECVIM-CA (pp. 120-123) (4 p.). 14th Symposium ECVIM-CA.
  Articles in volumes / proceedings - 2003
Bull, S., Laan, T.T.J.M., de Vrieze, G., Spee, B., van den Hoven, R. & Fink-Gremmels, J. (2003). Modulation of LPS-induved cytokine production by clenbuterol in equine alveolar macrophages. Journal of veterinary pharmacology and therapeutics (pp. 234-235) (2 p.).
  Awards - 0
B. Spee () Visiting Postdoctoral Fellowship
  Lectures - 2007
B. Spee (14.04.2007) Human progenitor cells are surrounded by different subtypes of hepatic stellate cells/myofibroblasts in human liver diseases in relation to differentiation and fibrosis patterns
B. Spee (06.10.2007) Keratin 19 positive hepatocellular carcinomas express various hepatic progenitor cell markers
  Doctoral thesis 1 (Research UU / Graduation UU) - 2006
Spee, B. (06.04.2006). Regenerative and Fibrotic Pathways in Canine Liver Disease. (224 p.).
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Gegenereerd op 2017-09-23 23:46:26

Completed projects

Project:
Liver progenitor cells for functional recovery of liver disease 01.01.2010 to 01.01.2016
General project description

In both veterinary and human medicine, liver disease is difficult to treat. Even with a clear diagnosis many treatment options fail and there is no curative therapy available. The adult liver harbours a reserve population of cells, the liver stem or progenitor cells, that can repopulate the liver in case of severe or chronic injury. However, in many liver diseases this repair mechanisms is either too little or too late. Recent studies have shown a remarkable similarity between liver diseases of man and dog, not only in pathology but also in the reaction pattern of the progenitor cells upon liver damage. The research performed at the Department of Clinical Sciences of Companion Animals is focus on unraveling the mechanisms of liver progenitor cell activation. The ultimate goal is to target these intrinsic repair mechanisms and to accomplish functional recovery of liver disease using the patient’s own progenitor cells.


In dogs a congenital copper storage disease occurs, that results in chronic hepatitis and cirrhosis. This disease highly resembles Wilsons disease in man. New therapeutic strategies that arise from our research can quickly be applied to restore liver function in dogs with copper-induced liver injury. This will provide vital knowledge and expertise for translating new therapies to the human clinic (translational medicine). Our project therefore aims to bridge the gap between fundamental findings and clinical application in both veterinary and human medicine.

 
Role Researcher Funding
NWO grant: Translational adult stem cell research grant
Project members UU
Gegenereerd op 2017-09-23 23:46:27
Full name
dr. B. Spee Contact details
Prof. dr. H. Jakobgebouw

Yalelaan 108
Room 2017
3584 CM  UTRECHT
The Netherlands


Jeannette Donker-Voetgebouw

Yalelaan 104-106
Room 2.017
3584 CM  UTRECHT
The Netherlands


Phone number (direct) +31 30 253 2848
Postal address
Yalelaan 106
3584 CM    UTRECHT
The Netherlands
Gegenereerd op 2017-09-23 23:46:27
Last updated 03.11.2016