dr. B.A.J. (Bernard) Roelen
B.A.J.Roelen@uu.nl
Associate Professor
Department of Farm Animal Health
Gegenereerd op 2017-06-29 12:54:01


Profile

Bernard Roelen studied Biology at Utrecht University and subsequently performed a PhD study at the Hubrecht Institute (Utrecht) under the supervision of Christine Mummery. After obtaining his PhD degree he was appointed junior researcher at the same institute. Afterwards he worked as a postdoctoral fellow at Massachusetts General Hospital/ Harvard Medical School in Boston (USA). After returning to the Netherlands he worked as a postdoctoral researcher at the Netherlands Cancer Institute in Amsterdam. In 2003 he became Assistant professor and in 2010 Associate professor at the Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University in Utrecht, the Netherlands. His research is focused on mammalian germ cells, early embryos stem cells and. He is author of >70 scientific publications.

Together with Christine Mummery and Anja van de Stolpe he wrote the book ‘Stamcellen’ (in Dutch) published by Veen. In 2011 together with Christine Mummery, Anja van de Stolpe and Ian Wilmut he wrote an English updated version of this book ‘Stem Cells’ that was published by Elsevier. In the same year the book was awarded the ‘Medical Book Award’ by the British Medical Association. A renewed and updated edition of this book, written by Christine Mummery, Anja van de Stolpe, Hans Clevers and Bernard Roelen was published in 2014.

Current Research:

Life of all placental mammals starts with an oocyte (egg) that is ovulated from the ovary and subsequently fertilized. Before the oocyte can be fertilized it has to mature from a diploid ‘ primordial germ cell’ to a haploid cell that is ready to receive the sperm cell. This includes a specific type of cell division (meiosis) during which homologous recombination occurs and the number of chromosomes is reduced to one set (haploid). In oocytes meiosis is extremely asymmetrical and leads to only one functional cell and three polar bodies. Although during embryonic development millions of oocytes are formed and stored in the ovaries, only hundreds (in women max ~500) of oocytes are ovulated and have a chance to be fertilized while the large majority degenerates in the ovaries. In my lab the process of oocyte maturation is studied in order to understand how oocyte developmental competence is acquired.

The fertilized egg, called zygote, is a ‘totipotent’ cell’ meaning that it will give rise to the entire organism including extra-embryonic structures such as the embryonic part of the placenta. During the first cleavage divisions the newly formed cells differentiate and lose their developmental competence. At the time of implantation the embryo, called blastula, is composed of trophectoderm, primitive endoderm (hypoblast), and an inner cells mass (epiblast).  Only the cells of the inner cell mass can give rise to the fetus, these cells ‘pluripotent’. The other cells form extra-embryonic structures. When cells from the inner cell mass are cultured in vitro they can form pluripotent embryonic stem cells. In my lab we try to understand how cells of early embryos lose, acquire or maintain developmental potency. This includes research on stem cells.

Lab:   

Technicians:

Leni van Tol; head lab

Eric Schoevers

Arend Rijneveld

Christine Oei

 

PhD students:
Karen Uhde

Minjie Tan

Bo Yu

 

Past Members:

Bas Brinkhof

Mahdi Mahdipour

Hilde Aardema

 

Leonie du Puy

Ewart Kuijk

Karlijn Wilschut

Jurriaan Hölzenspies

Juliëtte van den Dolder

Regiane Santos

Patricia Silva

Francisco Rubio Pomar

 

Collaborations:

Susana Chuva de Sousa Lopes

Leiden University Medical Center, Leiden, the Netherlands

 

Rene Ketting

Institute of Molecular Biology, Mainz, Germany

 

Andras Dinnyes

Biotaletum, Gödöllö, Hungary


Gabriela Rodrigues

Solveig Thorsteinsdottir

Faculty of Sciences, Lisbon University, Lisbon, Portugal

 

 

Strategic themes / Focus areas


Gegenereerd op 2017-06-29 12:54:02
Curriculum vitae

Academic appointments:

2010-

Associate professor

Department of Farm Animal Health, Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.

Research is focussed on the loss and gain of (puri)potency in germ cells, early embryos and stem cells.

2003-2010     

Assistant professor, Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.

 

Postdoctoral training

2002-2003     

Postdoctoral researcher, Division of Cellular Biochemistry, Netherlands Cancer Institute, Amsterdam the Netherlands. Group Peter ten Dijke

2001-2002     

Postdoctoral researcher, Renal Unit,  Massachusetts General Hospital and Harvard Medical School, Boston MA, USA. Group Herbert Y. Lin

1997-2000     

Junior researcher, Hubrecht Laboratory, Utrecht, the Netherlands. Groups Jacqueline Deschamps and Kirstie Lawson.

 

International work visits:

1995                           

Visiting scientist, Wellcome CRC Institute for Cancer and Developmental Biology, Cambridge UK. Group Anne McLaren

 

PhD degree:

1993-1997      

Hubrecht Laboratory, Utrecht

Promotor: Siegfried de Laat; copromotor: Christine Mummery

Thesis titel: TGF-βs and their receptors in mammalian development

 

Education:

1986-1992      

MSc Biology, Utrecht University

Gegenereerd op 2017-06-29 12:54:02
All publications
  Articles - 2017
Brinkhof, Bas, van Tol, Helena T A, Groot Koerkamp, Marian J A, Wubbolts, Richard W, Haagsman, Henk P & Roelen, Bernard A J (2017). Characterization of bovine embryos cultured under conditions appropriate for sustaining human naïve pluripotency. PLoS One, 12 (2), (pp. e0172920).
Aardema, Hilde, van Tol, Helena T A, Wubbolts, Richard W, Brouwers, Jos F H M, Gadella, Bart M & Roelen, Bernard A J (2017). Stearoyl-CoA desaturase activity in bovine cumulus cells protects the oocyte against saturated fatty acid stress. Biology of Reproduction © 2017 Society for the Study of Reproduction. Published by Oxford University Press..
Secher, Jan O, Ceylan, Ahmet, Mazzoni, Gianluca, Mashayekhi, Kaveh, Li, Tong, Muenthaisong, Suchitra, Nielsen, Troels T, Li, Dong, Li, Shengting, Petkov, Stoyan, Cirera, Susanna, Luo, Yonglun, Thombs, Lori, Kadarmideen, Haja N, Dinnyes, Andras, Bolund, Lars, Roelen, Bernard A J, Schmidt, Mette, Callesen, Henrik, Hyttel, Poul & Freude, Kristine K (03.01.2017). Systematic in vitro and in vivo characterization of Leukemia-inhibiting factor- and Fibroblast growth factor-derived porcine induced pluripotent stem cells. Molecular Reproduction and Development This article is protected by copyright. All rights reserved..
  Articles - 2016
Schoevers, Eric J, Santos, Regiane R, Fink-Gremmels, Johanna & Roelen, Bernard A J (2016). Toxicity of beauvericin on porcine oocyte maturation and preimplantation embryo development. Reproductive Toxicology, 65, (pp. 159-169) (11 p.). Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved..
  Articles - 2015
Brinkhof, Bas, van Tol, Helena T A, Groot Koerkamp, Marian J A, Riemers, Frank M, IJzer, Sascha G, Mashayekhi, Kaveh, Haagsman, Henk P & Roelen, Bernard A J (2015). A mRNA landscape of bovine embryos after standard and MAPK-inhibited culture conditions - a comparative analysis. BMC Genomics, 16, (pp. 277).
Hendriks - Onstein, Karin, Roelen, B A J, Colenbrander, B & Stout, T A E (2015). Cellular damage suffered by equine embryos after exposure to cryoprotectants or cryopreservation by slow-freezing or vitrification. Equine Veterinary Journal, 47 (6), (pp. 701-7) (7 p.). © 2014 EVJ Ltd..
Chuva de Sousa Lopes, S.M. & Roelen, B.A.J. (2015). Current status of in vitro differentiation of stem cells into gametes. Animal Reproduction, 12 (1), (pp. 46-51) (6 p.). Cited By :1 Export Date: 15 January 2016.
Aardema, Hilde, Gadella, Bart M, van de Lest, Chris H A, Brouwers, Jos F H M, Stout, Tom A E, Roelen, Bernard A J & Vos, Peter L A M (30.01.2015). Free fatty acid levels in fluid of dominant follicles at the preferred insemination time in dairy cows are not affected by early postpartum fatty acid stress. Journal of Dairy Science, 98 (4), (pp. 2322-2336) (15 p.). Copyright © 2015 American Dairy Science Association. Published by Elsevier Inc. All rights reserved..
Hendriks, W Karin, Colleoni, Silvia, Galli, Cesare, Paris, Damien B B P, Colenbrander, Ben, Roelen, Bernard A J & Stout, Tom A E (17.04.2015). Maternal age and in vitro culture affect mitochondrial number and function in equine oocytes and embryos. Reproduction, Fertility and Development, 27 (6), (pp. 957-968) (12 p.).
Roovers, Elke F, Rosenkranz, David, Mahdipour, Mahdi, Han, Chung-Ting, He, Nannan, Chuva de Sousa Lopes, Susana M, van der Westerlaken, Lucette A J, Zischler, Hans, Butter, Falk, Roelen, Bernard A J & Ketting, René F (2015). Piwi Proteins and piRNAs in Mammalian Oocytes and Early Embryos. Cell Reports, 10 (12), (pp. 2069-2082) (14 p.). Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved..
Mahdipour, Mahdi, Leitoguinho, Ana Rita Canhoto, Zacarias Silva, Ricardo A, van Tol, Helena T A, Stout, Tom A E, Rodrigues, Gabriela & Roelen, Bernard A J (2015). TACC3 Is Important for Correct Progression of Meiosis in Bovine Oocytes. PLoS One, 10 (7) (20 p.).
Lolicato, Francesca, Brouwers, Jos F., van de Lest, Chris H.A., Wubbolts, Richard, Aardema, Hilde, Priore, Paola, Roelen, Bernard A.J., Helms, J. Bernd & Gadella, Bart M (2015). The Cumulus Cell Layer Protects Bovine Maturing Oocyte Against Fatty Acid-Induced Lipotoxicity. Biology of Reproduction, 92 (1) (16 p.).
Santos, R. R., Schoevers, E. J., Wu, X., Roelen, B. A. J. & Fink-Gremmels, J. (2015). The protective effect of follicular fluid against the emerging mycotoxins alternariol and beauvericin. World Mycotoxin Journal, 8 (4), (pp. 445-450) (6 p.).
Mahdipour, Mahdi, van Tol, Helena T A, Stout, Tom A E & Roelen, Bernard A J (2015). Validating reference microRNAs for normalizing qRT-PCR data in bovine oocytes and preimplantation embryos. BMC Developmental Biology, 15.
Mahdipour, M., Van Tol, H. T. A., Stout, T. A. E. & Roelen, B. A. J. (04.12.2015). VALIDATION OF REFERENCE microRNAs FOR NORMALIZING EXPRESSION DATA GENERATED BY QUANTITATIVE PCR. Reproduction, Fertility and Development, 27 (1), (pp. 202) (1 p.).
  Articles - 2014
Solak, Kamila A., Santos, Regiane R., van den Berg, Martin, Blaauboer, Bas J., Roelen, Bernard A J & van Duursen, Majorie B M (2014). Naringenin (NAR) and 8-prenylnaringenin (8-PN) reduce the developmental competence of porcine oocytes in vitro. Reproductive Toxicology, 49, (pp. 1-11) (11 p.).
Poot, M.J.M., Roelen, B.A.J. & Piersma, T. (2014). Red knots scavenging on large, dying cockles: Opportunistic feeding by a sensory specialized mollusc-crushing shorebird. Wader Study Group Bulletin, 121 (1), (pp. 33-42) (10 p.). Export Date: 15 January 2016.
Almouzni, Geneviève, Altucci, Lucia, Amati, Bruno, Ashley, Neil, Baulcombe, David, Beaujean, Nathalie, Bock, Christoph, Bongcam-Rudloff, Erik, Bousquet, Jean, Braun, Sigurd, Bressac-de Paillerets, Brigitte, Bussemakers, Marion, Clarke, Laura, Conesa, Ana, Estivill, Xavier, Fazeli, Alireza, Grgurević, Neža, Gut, Ivo, Heijmans, Bastiaan T, Hermouet, Sylvie, Houwing-Duistermaat, Jeanine, Iacobucci, Ilaria, Ilaš, Janez, Kandimalla, Raju, Krauss-Etschmann, Susanne, Lasko, Paul, Lehmann, Sören, Lindroth, Anders, Majdič, Gregor, Marcotte, Eric, Martinelli, Giovanni, Martinet, Nadine, Meyer, Eric, Miceli, Cristina, Mills, Ken, Moreno-Villanueva, Maria, Morvan, Ghislaine, Nickel, Dörthe, Niesler, Beate, Nowacki, Mariusz, Nowak, Jacek, Ossowski, Stephan, Pelizzola, Mattia, Pochet, Roland, Potočnik, Uroš, Radwanska, Magdalena, Raes, Jeroen, Rattray, Magnus, Robinson, Mark D, Roelen, Bernard, Sauer, Sascha, Schinzer, Dieter, Slagboom, Eline, Spector, Tim, Stunnenberg, Hendrik G, Tiligada, Ekaterini, Torres-Padilla, Maria-Elena, Tsonaka, Roula, Van Soom, Ann, Vidaković, Melita & Widschwendter, Martin (2014). Relationship between genome and epigenome--challenges and requirements for future research. BMC Genomics, 15 (7 p.).
Santos, Regiane R, Schoevers, Eric J & Roelen, Bernard Aj (2014). Usefulness of bovine and porcine IVM/IVF models for reproductive toxicology. Reproductive Biology and Endocrinology, 12 (1), (pp. 12).
  Articles - 2013
Aardema, Hilde, Lolicato, Francesca, van de Lest, Chris H A, Brouwers, Jos F, Vaandrager, Arie B, van Tol, Helena T A, Roelen, Bernard A J, Vos, Peter L A M, Helms, J Bernd & Gadella, Bart M (2013). Bovine cumulus cells protect maturing oocytes from increased fatty acid levels by massive intracellular lipid storage. Biology of Reproduction, 88 (6), (pp. 1-15) (15 p.).
Mummery, C.L. & Roelen, B.A.J. (2013). Cloning human embryos. Nature, 498, (pp. 174-175) (2 p.).
Merton, J.S., Knijn, H.M., Flapper, H., Dotinga, F., Roelen, B.A.J., Vos, P.L.A.M. & Mullaart, E. (2013). Cysteamine supplementation during in vitro maturation of slaughterhouse- and opu-derived bovine oocytes improves embryonic development without affecting cryotolerance, pregnancy rate, and calf characteristics. Theriogenology, 80 (4), (pp. 365-371) (7 p.).
Aardema, Hilde, Roelen, Bernard A J, van Tol, Helena T A, Oei, Christine H Y, Gadella, Bart M & Vos, Peter L A M (2013). Follicular 17β-estradiol and progesterone concentrations and degree of cumulus cell expansion as predictors of in vivo-matured oocyte developmental competence in superstimulated heifers. Theriogenology, 80 (6), (pp. 576-83) (8 p.). Copyright © 2013 Elsevier Inc. All rights reserved..
Santos, R. R., Schoevers, E. J., Roelen, B. A J, Fink-Gremmels, J. & Schoevers, Eric (01.08.2013). Mycotoxins and female reproduction - In vitro approaches. World Mycotoxin Journal, 6 (3), (pp. 245-253) (9 p.).
Santos, R.R., Schoevers, E.J., Roelen, B.A.J. & Fink-Gremmels, J. (2013). Mycotoxins and female reproduction: in vitro approaches. World Mycotoxin Journal, 6 (3), (pp. 245-253) (9 p.).
  Articles - 2012
Merton, J.S., de Roos, A.P.W., Koenen, E.P.C., Roelen, B.A.J., Vos, P.L.A.M., Mullaart, E. & Knijn, H.M. (2012). Bovine OPU-Derived Oocytes can be Matured In Vitro for 16–28 h with Similar Developmental Capacity. Reproduction in Domestic Animals, 47, (pp. 1037-1042) (6 p.).
du Puy, Leonie, Beqqali, Abdelaziz, van Tol, Helena T A, Monshouwer-Kloots, Jantine, Passier, Robert, Haagsman, Henk P & Roelen, Bernard A J (2012). Sarcosin (Krp1) in skeletal muscle differentiation - gene expression profiling and knockdown experiments. International Journal of Developmental Biology, 56 (4), (pp. 301-9) (9 p.).
Kuijk, Ewart W, van Tol, Leni T A, Van de Velde, Hilde, Wubbolts, Richard, Welling, Maaike, Geijsen, Niels & Roelen, Bernard A J (2012). The roles of FGF and MAP kinase signaling in the segregation of the epiblast and hypoblast cell lineages in bovine and human embryos. Development (Cambridge), 139 (5), (pp. 871-882) (12 p.).
Schoevers, Eric J., Santos, Regiane R., Colenbrander, Ben, Fink-Gremmels, Johanna & Roelen, Bernard A J (01.08.2012). Transgenerational toxicity of Zearalenone in pigs. Reproductive Toxicology, 34 (1), (pp. 110-119) (10 p.).
  Articles - 2011
Hölzenspies, J.J., Stoorvogel, W., Colenbrander, B., Roelen, B.A.J. & van Haeften, T. (2011). A methode for immediate comparative assessment of microinjected mammalian oocytes. Theriogenology, 75, (pp. 386-391) (6 p.).
Wilschut, Karlijn J, van Tol, Helena T A, Arkesteijn, Ger J A, Haagsman, Henk P & Roelen, Bernard A J (2011). Alpha 6 integrin is important for myogenic stem cell differentiation. Stem Cell Research, 7 (2), (pp. 112-123) (12 p.). Copyright © 2011 Elsevier B.V. All rights reserved..
du Puy, L., Lopes, S.M., Haagsman, H.P. & Roelen, B.A.J. (2011). Analysis of co-expression of OCT4, NANOG and SOX2 in pluripotent cells of the porcine embryo, in vivo and in vitro. Theriogenology, 75 (3), (pp. 513-526) (14 p.).
Paris, D.B.B.P., Kuijk, E.W., Roelen, B.A.J. & Stout, T.A.E. (2011). Establishing reference genes for use in real-time quantitative PCR analysis of early equine embryos. Reproduction, Fertility and Development, 23, (pp. 353-363) (11 p.).
Roelen, B.A.J. (2011). Functional Germ Cells: The Power of Soma. Biology of Reproduction, 84, (pp. 619-620) (2 p.).
Verbraak, E.J.C., van 't Veld, E.M., Groot Koerkamp, M.J.A., Roelen, B.A.J., van Haeften, T., Stoorvogel, W. & Zijlstra, C. (2011). Identification of genes targeted by FSH and oocytes in porcine granulosa cells. Theriogenology, 75, (pp. 362-376) (15 p.).
Yindee, M., Techakumphu, M., Lohachit, C., Sirivaidyapong, S., Na-Chiangmai, A., Roelen, B.A.J. & Colenbrander, B. (2011). Maturation Competence of Swamp Buffalo Oocytes Obtained by Ovum Pick-Up and from Slaughterhouse Ovaries. Reproduction in Domestic Animals, 46 (5), (pp. 824-831) (8 p.).
Roelen, B.A.J. (2011). Of Stem Cells and Germ Cells. Reproduction in Domestic Animals, 46, (pp. 53-59) (7 p.).
Aardema, H., Vos, P.L.A.M., Lolicato, F., Roelen, B.A.J., Knijn, H.M., Vaandrager, A.B., Helms, J.B. & Gadella, B.M. (2011). Oleic Acid Prevents Detrimental Effects of Saturated Fatty Acids on Bovine Oocyte. Biology of Reproduction, 85 (1), (pp. 62-69) (8 p.).
Roelen, B.A.J. & Chuva de Sousa Lopes, S.M. (2011). Origins of pluripotent stem cells. Minerva Ginecologica, 63 (4), (pp. 351-363) (13 p.).
Kuijk, E.W., Lopes, S.M., Geijsen, N., Macklon, N.S. & Roelen, B.A.J. (2011). The different shades of mammalian pluripotent stem cells. Human Reproduction Update, 17 (2), (pp. 254-271) (19 p.). doi:10.1093/humupd/dmq035.
Chuva de Sousa Lopes, S.M. & Roelen, B.A.J. (2011). The way of the germline, its developmental cycle and epigenetic network. Differentiation, 81, (pp. 217-221) (5 p.).
  Articles - 2010
Kuijk, E.W., de Gier, J., Lopes, S.M., Chambers, I., van Pelt, A.M.M., Colenbrander, B. & Roelen, B.A.J. (2010). A distinct expression pattern in mammalian testes indicates a conserved role for NANOG in spermatogenesis. PLoS One, 5, (pp. e10987) (1 p.).
Lopes, S.M. & Roelen, B.A.J. (2010). An overview on the diversity of cellular organelles during the germ cell cycle. Histology and Histopathology, 25, (pp. 267-276) (10 p.).
Hölzenspies, J.J., Roelen, B.A.J., Colenbrander, B., Romijn, R.A.P., Hemrika, W., Stoorvogel, W. & van Haeften, T. (2010). Clathrin is essential for meiotic spindle function in oocytes. Reproduction, 140, (pp. 223-233) (11 p.).
du Puy, L., Lopes, S.M., Haagsman, H.P. & Roelen, B.A.J. (2010). Differentiation of porcine inner cell mass cells into proliferating neural cells. Stem Cells and Development, 19, (pp. 61-70) (10 p.).
van Tol, Helena T A, Vernooij, Johannes C M, Colenbrander, Ben, Gutknecht, Dagmar, Macklon, Nicholas S & Roelen, Bernard A J (2010). Expression of leptin receptor mRNA in cumulus cells is correlated with expression of PTX3. Reproductive BioMedicine Online, 20 (6), (pp. 741-750) (10 p.). 2010 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved..
Wilschut, K.J., Haagsman, H.P. & Roelen, B.A.J. (2010). Extracellular matrix components direct porcine muscle stem cell behavior. Experimental Cell Research, 316 (3), (pp. 341-352) (12 p.).
Teklenburg, G., Salker, M., Molokhia, M., Lavery, S., Trew, G., Aojanepong, T., Mardon, H.J., Lokugamage, A.U., Rai, R., Landles, C., Roelen, B.A.J., Quenby, S., Kuijk, E.W., Kavelaars, A.M.A.A., Heijnen, C.J., Regan, L., Brosens, J.J. & Macklon, N.S. (2010). Natural selection of human embryos: decidualizing endometrial stromal cells serve as sensors of embryo quality upon implantation. PLoS One, 5 (4), (pp. e10258) (6 p.).
Salker, M., Teklenburg, G., Molokhia, M., Lavery, S., Trew, G., Aojanepong, T., Mardon, H.J., Lokugamage, A.U., Rai, R., Landles, C., Roelen, B.A.J., Quenby, S., Kuijk, E.W., Kavelaars, A., Heijnen, C.J., Regan, L., Macklon, N.S. & Brosens, J.J. (2010). Natural selection of human embryos: impaired decidualization of endometrium disables embro-maternal interactions and causes recurrent pregnancy loss. PLoS One, 5 (4), (pp. e10287) (7 p.).
Lopes, S.M. & Roelen, B.A.J. (2010). On the formation of germ cells: The good, the bad and the ugly. Differentiation, 79, (pp. 131-140) (10 p.).
Schoevers, E.J., Fink-Gremmels, J., Colenbrander, B. & Roelen, B.A.J. (01.10.2010). Porcine oocytes are most vulnerable to the mycotoxin deoxynivalenol during formation of the meiotic spindle. Theriogenology, 74 (6), (pp. 968-978) (11 p.).
Schoevers, E.J., Fink-Gremmels, J., Colenbrander, B. & Roelen, B.A.J. (2010). Porcine oocytes are most vulnerable to the mycotoxin deoxynivalenol during formation of the meiotic spindle. Theriogenology, 74, (pp. 968-978) (10 p.).
Kuijk, E.W., van Mil, A., Brinkhof, B., Penning, L.C., Colenbrander, B. & Roelen, B.A.J. (2010). PTEN and TRP53 independently suppress Nanog expression in spermatogonial stem cells. Stem Cells and Development, 19, (pp. 979-988) (10 p.).
  Articles - 2009
du Puy, L., Beqqali, A., Monshouwer-Kloots, J., Haagsman, H.P., Roelen, B.A.J. & Passier, R. (2009). CAZIP, A Novel Protein Expressed in the Developing Heart and Nervous System. Developmental Dynamics, 238, (pp. 2903-2911) (9 p.).
Hölzenspies, J.J., Stoorvogel, W., Colenbrander, B., Roelen, B.A.J., Gutknecht, D.R. & van Haeften, T. (2009). CDC2/SPDY transiently associates with endoplasmic reticulum exit sites during oocyte maturation. BMC Developmental Biology, 9, (pp. 8) (16 p.).
Santos, Regiane R, Knijn, Hiemke M, Vos, Peter L A M, Oei, Christine H Y, van Loon, Thijs, Colenbrander, Ben, Gadella, Bart M, van den Hurk, Rob & Roelen, Bernard A J (2009). Complete follicular development and recovery of ovarian function of frozen-thawed, autotransplanted caprine ovarian cortex. Fertility and Sterility, 91 (4 Suppl), (pp. 1455-1458) (4 p.).
Kuijk, E.W., Colenbrander, B. & Roelen, B.A.J. (2009). The effects of growth factors on in vitro-cultured porcine testicular cells. Reproduction, 138 (4), (pp. 721-731) (11 p.).
  Articles - 2008
Zijlstra, C., Kidson, A., Schoevers, E. J., Daemen, A. J J M, Tharasanit, T., Kuijk, E. W., Hazeleger, W., Ducro-Steverink, D. W B, Colenbrander, B., Roelen, B. A J & Schoevers, Eric (01.10.2008). Blastocyst morphology, actin cytoskeleton quality and chromosome content are correlated with embryo quality in the pig. Theriogenology, 70 (6), (pp. 923-935) (13 p.).
Kuijk, Ewart W, Du Puy, Leonie, Van Tol, Helena T A, Oei, Christine H Y, Haagsman, Henk P, Colenbrander, Ben & Roelen, Bernard A J (2008). Differences in early lineage segregation between mammals. Developmental Dynamics, 237 (4), (pp. 918-927) (10 p.). (c) 2008 Wiley-Liss, Inc..
van Tol, Helena T A, van Eerdenburg, Frank J C M, Colenbrander, Ben & Roelen, Bernard A J (2008). Enhancement of Bovine oocyte maturation by leptin is accompanied by an upregulation in mRNA expression of leptin receptor isoforms in cumulus cells. Molecular Reproduction and Development, 75 (4), (pp. 578-587) (10 p.). Copyright 2007 Wiley-Liss, Inc..
Wilschut, K.J., Jaksani, S., van den Dolder, J., Haagsman, H.P. & Roelen, B.A.J. (2008). Isolation and characterization of porcine adult muscle-derived progenitor cells. Journal of Cellular Biochemistry, 105, (pp. 1228-1239) (12 p.).
Roelen, B.A.J. & Lopes, S.M. (2008). Of stem cells and gametes: similarities and differences. Current Medicinal Chemistry, 15, (pp. 1249-1256) (8 p.).
Santos, R.R., van Haeften, T., Roelen, B.A.J., Knijn, H.M., Colenbrander, B., Gadella, B.M. & van den Hurk, R. (2008). Osmotic tolerance and freezability of isolate caprine ealy-staged follicles. Cell and Tissue Research, 333 (2), (pp. 323-331) (9 p.).
Lopes, S.M. & Roelen, B.A.J. (2008). Primordial germ cell specification: the importance of being 'blimped'. Histology and Histopathology, 23, (pp. 1553-1561) (9 p.).
  Articles - 2007
Schoevers, E. J., Colenbrander, B., Roelen, B. A J & Schoevers, Eric (01.04.2007). Developmental stage of the oocyte during antral follicle growth and cumulus investment determines in vitro embryo development of sow oocytes. Theriogenology, 67 (6), (pp. 1108-1122) (15 p.).
da Silva, P.F.N., Gadella, B.M., Colenbrander, B. & Roelen, B.A.J. (2007). Exposure of bovine sperm to pro-oxidants impairs the developmental competence of the embryo after the first cleavage. Theriogenology, 67, (pp. 609-619) (11 p.).
Malekinejad, Hassan, Schoevers, Eric J., Daemen, Ineke J J M, Zijlstra, Carla, Colenbrander, Ben, Fink-Gremmels, Johanna & Roelen, Bernard A J (01.11.2007). Exposure of oocytes to the Fusarium toxins zearalenone and deoxynivalenol causes aneuploidy and abnormal embryo development in pigs. Biology of Reproduction, 77 (5), (pp. 840-847) (8 p.).
Malekinejad, H., Schoevers, E.J., Daemen, A.J.J.M., Zijlstra, C., Fink-Gremmels, J., Colenbrander, B. & Roelen, B.A.J. (2007). Exposure of oocytes to the fusarium toxins zearalenone and deoxynivalenol causes aneuploidy ans abnormal embryo development in pigs. Biology of Reproduction, 77, (pp. 840-847) (8 p.).
Kuijk, Ewart W, du Puy, Leonie, van Tol, Helena T A, Haagsman, Henk P, Colenbrander, Ben & Roelen, Bernard A J (2007). Validation of reference genes for quantitative RT-PCR studies in porcine oocytes and preimplantation embryos. Developmental Biology, 7 (58), (pp. 58) (8 p.).
  Articles - 2006
Beker-van Woudenberg, Anna R, Zeinstra, Elly C, Roelen, Bernard A J, Colenbrander, Ben & Bevers, Mart M (2006). Developmental competence of bovine oocytes after specific inhibition of MPF kinase activity - effect of estradiol supplementation and follicle size. Animal Reproduction Science, 92 (3-4), (pp. 231-40) (10 p.).
Beker-van Woudenberg, A.R., Zeinstra, E.C., Roelen, B.A.J., Colenbrander, B. & Bevers, M.M. (2006). Developmental competence of bovine oocytes after specific inhibition of MPF kinase activity: effect of estradiol supplementation and follicle size. Animal Reproduction Science, 92 (3-4), (pp. 231-240) (10 p.).
Fatehi, A. N., Bevers, M. M., Schoevers, E., Roelen, B. A J, Colenbrander, B. & Gadella, B. M. (01.03.2006). DNA damage in bovine sperm does not block fertilization and early embryonic development but induces apoptosis after the first cleavages. Journal of Andrology, 27 (2), (pp. 176-188) (13 p.).
Silva, J R V, van den Hurk, R, van Tol, H T A, Roelen, B A J & Figueiredo, J R (2006). The Kit ligand/c-Kit receptor system in goat ovaries - gene expression and protein localization. Zygote, 14 (4), (pp. 317-28) (12 p.).
  Articles - 2005
Rubio Pomar, F.J., Teerds, K.J., Kidson, A., Colenbrander, B.A., Tharasanit, T., Aguilar, B. & Roelen, B.A.J. (2005). Differences in the incidence of apoptosis between in vivo and in vitro produced blastocysts of farm animal species: a comparitive study. Theriogenology, 63 (8), (pp. 2254-2268) (15 p.).
Fatehi, A N, van den Hurk, R, Colenbrander, B, Daemen, A J J M, van Tol, H T A, Monteiro, R M, Roelen, B A J & Bevers, M M (2005). Expression of bone morphogenetic protein2 (BMP2), BMP4 and BMP receptors in the bovine ovary but absence of effects of BMP2 and BMP4 during IVM on bovine oocyte nuclear maturation and subsequent embryo development. Theriogenology, 63 (3), (pp. 872-889) (18 p.).
Silva, J R V, van den Hurk, R, van Tol, H T A, Roelen, B A J & Figueiredo, J R (2005). Expression of growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15), and BMP receptors in the ovaries of goats. Molecular Reproduction and Development, 70 (1), (pp. 11-19) (9 p.). (c) 2004 Wiley-Liss, Inc..
Passier, R., Ward-van Oostwaard, D., Snapper, J., Kuijk, E.W., Roelen, B.A.J., Kloots, J., Hassink, R., Brutel de la Rivière, A. & Mummery, C.L. (2005). Increased cardiomyocyte differentiation from human embryonic stem cells in serum-free cultures. Stem Cells, 23, (pp. 772-780) (9 p.).
Schoevers, E. J., Bevers, M. M., Roelen, B. A J, Colenbrander, B. & Schoevers, Eric (01.03.2005). Nuclear and cytoplasmic maturation of sow oocytes are not synchronized by specific meiotic inhibition with roscovitine during in vitro maturation. Theriogenology, 63 (4), (pp. 1111-1130) (20 p.).
Fatehi, A. Nader, Roelen, Bernard A J, Colenbrander, Ben, Schoevers, Eric J., Gadella, Bart M., Bevers, Mart M. & Van Den Hurk, Rob (01.05.2005). Presence of cumulus cells during in vitro fertilization protects the bovine oocyte against oxidative stress and improves first cleavage but does not affect further development. Zygote, 13 (2), (pp. 177-185) (9 p.).
  Articles - 2004
Lopes, S.M., Roelen, B.A.J., Monteiro, R.M., Emmens, R., Lin, H.Y., Li, E., Lawson, K.A. & Mummery, C.L. (2004). BMP signaling mediated by ALK2 in the visceral endoderm is necessary for the generation of primordial germ cells in the mouse embryo. Genes and Development, 18 (15), (pp. 1838-1849) (12 p.).
Beker-van Woudenberg, Anna R, van Tol, Helena T A, Roelen, Bernard A J, Colenbrander, Ben & Bevers, Mart M (2004). Estradiol and its membrane-impermeable conjugate (estradiol-bovine serum albumin) during in vitro maturation of bovine oocytes - effects on nuclear and cytoplasmic maturation, cytoskeleton, and embryo quality. Biology of Reproduction, 70 (5), (pp. 1465-74) (10 p.).
Costa-Lage Beker, A.R., van Tol, H.T.A., Roelen, B.A.J., Colenbrander, B.A. & Bevers, M.M. (2004). Estradiol and its membrane-impermeable conjugate (estradiol-bovine serum albumin) during in vitro maturation of bovine oocytes: effects on nuclear and cytoplasmic maturation, cytoskeleton, and embryo quality. Biology of Reproduction, 70 (5), (pp. 1465-1474) (10 p.).
Silva, J R V, van den Hurk, R, van Tol, H T A, Roelen, B A J & Figueiredo, J R (2004). Gene expression and protein localisation for activin-A, follistatin and activin receptors in goat ovaries. Journal of Endocrinology, 183 (2), (pp. 405-15) (11 p.).
Silva, J.R.V., van den Hurk, R., van Tol, H.T.A., Roelen, B.A.J. & Figueiredo, J.R. (2004). Gene expression and protein localisation for activin-A, follistatin, and activin receptors in goat ovaries. Journal of Endocrinology, 183, (pp. 405-415) (11 p.).
Rubio Pomar, F.J., Roelen, B.A.J., Slot, K.A., van Tol, H.T.A., Colenbrander, B.A. & Teerds, K.J. (2004). Role of Fas-Mediated Apoptosis and Follicle-Stimularing Hormone on the development capacity of bovine cumulus oocyte complexes in vitro. Biology of Reproduction, 71 (3), (pp. 790-796) (7 p.).
Rubio Pomar, Francisco J, Roelen, Bernard A J, Slot, Karin A, van Tol, Helena T A, Colenbrander, Ben & Teerds, Katja J (2004). Role of Fas-mediated apoptosis and follicle-stimulating hormone on the developmental capacity of bovine cumulus oocyte complexes in vitro. Biology of Reproduction, 71 (3), (pp. 790-6) (7 p.).
van Bezooijen, R., Roelen, B.A.J. & Visser, A. (2004). Sclerostin is an osteocyte-expressed negative regulator of bone formation, but not a classical BMP antagonist. Journal of Experimental Medicine, 199 (6), (pp. 805-814) (10 p.).
  Books - 2014
Roelen, B.A.J., Mummery, Christine, van de Stolpe, Anja & Clevers, Hans (2014). stem cells - scientific facts and fiction. Elsevier.
  Books - 2010
Mummery, C.L., Wilmut, I., van de Stolpe, A. & Roelen, B.A.J. (2010). Stem Cells: scientific facts and fiction. Amsterdam: Elsevier.
  Books - 2006
Mummery, C.L., van de Stolpe, A. & Roelen, B.A.J. (2006). Stamcellen. Amsterdam: Veen Magazines.
  Book parts / chapters - 2015
Duijvestijn, M.B.H.M., van der Woude, J.W., Bijleveld, C., Geelen, M.J.H., van Haeften, T., Tielens, A.G.M., van Dongen, A.M., Piek, C.J., Prins, M., Teske, E., j p h m, vossen, Gadella, B.M., Lam, T.J.G.M., Rijneveld, A., Roelen, B.A.J., Beitsma, M.M., Egberink, H.F., van Dijk, M.A.M., Nijsse, E.R., Ploeger, H.W. & Veneberg, R.E. (2015). laboratoriumdiagnostiek - een praktische handleiding. In jael kool-van der woude (Eds.), laboratoriumdiagnostiek - een praktische handleiding (pp. 10-15,163-167,175-185) (22 p.). Ponsen & Looijen.
  Book parts / chapters - 2013
Brinkhof, B., Roelen, B.A.J. & Haagsman, H.P. (2013). Meet the Stem Cells. Engineering aspects of food biotechnology
  Reports - 2011
Roelen, B.A.J. & Chuva de Sousa Lopes, S.M. (2011). 1st Royan Institute International Summer School on Developmental Biology and Stem Cells. (4 p.). UBC Press.
  Awards - 0
B.A.J. Roelen (0). BMA board of science awards for the public understanding of science. BMA Medical Book Award.
B.A.J. Roelen (0). FRIGGA award.
  Lectures - 0
B.A.J. Roelen (0). 1st Royal Institute International Summer School: Developmental Biology and Stem Cells. teacher.
B.A.J. Roelen (0). COST Joint Workshop on Relationship between Epigenome and Genome. Brussels, Belgium, Lezing.
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Gegenereerd op 2017-06-29 12:54:02

 

Completed projects

Project:
Kisspeptin: the endocrinological gatekeeper to reproductive function. A realistic target for non-surgical contraception in the dog. 03.01.2011 to 30.12.2016
General project description
 
Role Researcher Funding
External funding: Found animal Foundation (2 years)
Project members UU
Gegenereerd op 2017-06-29 12:54:02
Additional functions and activities

Board member Vereniging voor Fertiliteitsstudie (VFS; Society for Fertility Study)

http://www.vfs-online.org/

Treasurer Dutch Society for Developmental Biology

http://www.nedvob.net/index.php/

 

Editorial board scientific journals:

BMC Developmental Biology

PLoS ONE

Stem Cells International

Gegenereerd op 2017-06-29 12:54:02
Full name
dr. B.A.J. Roelen Contact details
Martinus G. de Bruingebouw

Yalelaan 7
Room 2050
3584 CL  UTRECHT
The Netherlands


Jeannette Donker-Voetgebouw

Yalelaan 104-106
Room 2.050
3584 CM  UTRECHT
The Netherlands


Phone number (direct) +31 30 253 3352

http://roelenlab.weebly.com

 

Gegenereerd op 2017-06-29 12:54:02
Last updated 13.04.2017