Nature-Based Solutions have the potential to limit impacts of climate change, enhance biodiversity and improve environmental quality while contributing to economic activities and social well-being. Examples are green roofs and city parks that limit heat stress, city lagoons that store water and permeable surfaces, vegetation and rain gardens to intercept storm water. To unlock the potential of nature based solutions for sustainable urban development, NATURVATION takes a transdisciplinary, internationally comparative approach to capture the multiple impacts and values of these solutions. Based on a comparison of 1000 nature based solutions across 6 cities, it identifies the most promising governance, business, finance and participation models and how to overcome the systemic conditions that currently limit their use. The real-world impact of NATURVATION is realized through co-design, co-development and co-implementation of new partnerships, knowledge, processes and tools required to build capacity, enable replication and foster cultural change.