Publicaties
2021
Wetenschappelijke publicaties
Kyle, J. E., Aimo, L., Bridge, A. J., Clair, G., Fedorova, M., Helms, J. B., Molenaar, M. R., Ni, Z., Orešič, M., Slenter, D., Willighagen, E., & Webb-Robertson, B-J. M. (2021). Interpreting the lipidome: bioinformatic approaches to embrace the complexity. Metabolomics, 17(6), 1-10. [55]. https://doi.org/10.1007/s11306-021-01802-6
Sirati, N., Popova, B., Molenaar, M. R., Verhoek, I. C., Braus, G. H., Kaloyanova, D. V., & Helms, J. B. (2021). Dynamic and Reversible Aggregation of the Human CAP Superfamily Member GAPR-1 in Protein Inclusions in Saccharomyces cerevisiae. Journal of Molecular Biology, 433(19), [167162]. https://doi.org/10.1016/j.jmb.2021.167162
Molenaar, M. R., Yadav, K. K., Toulmay, A., Wassenaar, T. A., Mari, M. C., Caillon, L., Chorlay, A., Lukmantara, I. E., Haaker, M. W., Wubbolts, R. W., Houweling, M., Vaandrager, A. B., Prieur, X., Reggiori, F., Choudhary, V., Yang, H., Schneiter, R., Thiam, A. R., Prinz, W. A., & Helms, J. B. (2021). Retinyl esters form lipid droplets independently of triacylglycerol and seipin. Journal of Cell Biology, 220(10), 1-14. [e202011071]. https://doi.org/10.1083/jcb.202011071
Zhang, M., Bromfield, E. G., Veenendaal, T., Klumperman, J., Helms, J. B., & Gadella, B. M. (2021). Characterization of different oligomeric forms of CRISP2 in the perinuclear theca versus the fibrous tail structures of boar spermatozoa. Biology of Reproduction, 105(5), 1160-1170. https://doi.org/10.1093/biolre/ioab145
Mantilla, M. J., Cabrera Díaz, C. E., Ariza-Aranguren, G., de Cock, H., Helms, J. B., Restrepo, S., Jiménez, E., & Celis Ramírez, A. M. (2021). Back to the Basics: Two Approaches for the Identification and Extraction of Lipid Droplets from Malassezia pachydermatis CBS1879 and Malassezia globosa CBS7966. Current Protocols, 1(5), 1-16. [e122]. https://doi.org/10.1002/cpz1.122
Sheng, J., Gadella, B. M., Olrichs, N. K., Kaloyanova, D. V., & Helms, J. B. (2021). The less conserved metal-binding site in human CRISP1 remains sensitive to zinc ions to permit protein oligomerization. Scientific Reports, 11(1), [5498]. https://doi.org/10.1038/s41598-021-84926-y
2020
Wetenschappelijke publicaties
Zhang, M., Zenezini-Chiozzi, R., Kaloyanova, D. V., Helms, J. B., & Gadella, B. M. (2020). Characterization of CRISP2 in ejaculated boar sperm and its involvement in oligomerization protein complex formation. Animal Reproduction Science, 220, [106416]. https://doi.org/10.1016/j.anireprosci.2020.106416
Molenaar, M. R., Penning, L. C., & Helms, J. B. (2020). Playing Jekyll and Hyde-The Dual Role of Lipids in Fatty Liver Disease. Cells, 9(10), [2244]. https://doi.org/10.3390/cells9102244
Sheng, J., Olrichs, N. K., Gadella, B. M., Kaloyanova, D. V., & Helms, J. B. (2020). Regulation of Functional Protein Aggregation by Multiple Factors: Implications for the Amyloidogenic Behavior of the CAP Superfamily Proteins. International Journal of Molecular Sciences, 21(18), [6530]. https://doi.org/10.3390/ijms21186530
Haaker, M. W., Vaandrager, A. B., & Helms, J. B. (2020). Retinoids in health and disease: A role for hepatic stellate cells in affecting retinoid levels. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1865(6), [158674]. https://doi.org/10.1016/j.bbalip.2020.158674
Haaker, M. W., Kruitwagen, H. S., Vaandrager, A. B., Houweling, M., Penning, L. C., Molenaar, M. R., van Wolferen, M. E., Oosterhoff, L. A., Spee, B., & Helms, J. B. (2020). Identification of potential drugs for treatment of hepatic lipidosis in cats using an in vitro feline liver organoid system. Journal of Veterinary Internal Medicine, 34(1), 132-138. https://doi.org/10.1111/jvim.15670
2019
Wetenschappelijke publicaties
Sheng, J., Olrichs, N. K., Geerts, W. J., Kaloyanova, D. V., & Helms, J. B. (2019). Metal ions and redox balance regulate distinct amyloid-like aggregation pathways of GAPR-1. Scientific Reports, 9(1), [15048]. https://doi.org/10.1038/s41598-019-51232-7
Molenaar, M. R., Jeucken, A., Wassenaar, T. A., van der Lest, C. H. A., Brouwers, J. F. H. M., & Helms, J. B. (2019). LION/web: a web-based ontology enrichment tool for lipidomic data analysis. GigaScience, 8(6), [giz061]. https://doi.org/10.1093/gigascience/giz061
Jeucken, A., Molenaar, M. R., van de Lest, C. H. A., Jansen, J. W. A., Helms, J. B., & Brouwers, J. F. H. M. (2019). A comprehensive functional characterization of Escherichia coli lipid genes. Cell Reports, 27(5), 1597-1606.e2. https://doi.org/10.1016/j.celrep.2019.04.018
Sheng, J., Olrichs, N. K., Geerts, W. J., Li, X., Rehman, A. U., Gadella, B. M., Kaloyanova, D. V., & Helms, J. B. (2019). Zinc binding regulates amyloid-like aggregation of GAPR-1. Bioscience Reports, 39(2). https://doi.org/10.1042/BSR20182345
van der Kant, R., Langness, V. F., Herrera, C. M., Williams, D. A., Fong, L. K., Leestemaker, Y., Steenvoorden, E., Rynearson, K. D., Brouwers, J. F., Helms, J. B., Ovaa, H., Giera, M., Wagner, S. L., Bang, A. G., & Goldstein, L. S. B. (2019). Cholesterol Metabolism Is a Druggable Axis that Independently Regulates Tau and Amyloid-β in iPSC-Derived Alzheimer's Disease Neurons. Cell Stem Cell, 24(3). https://doi.org/10.1016/j.stem.2018.12.013
van Elst, K., Brouwers, J. F., Merkens, J. E., Broekhoven, M. H., Birtoli, B., Helms, J. B., & Kas, M. J. H. (2019). Chronic dietary changes in n-6/n-3 polyunsaturated fatty acid ratios cause developmental delay and reduce social interest in mice. European Neuropsychopharmacology, 29(1), 16-31. https://doi.org/10.1016/j.euroneuro.2018.11.1106
2018
Wetenschappelijke publicaties
Molenaar, M., Jeucken, A., Wassenaar, T. A., van de Lest, C. H. A., Brouwers, J. F., & Helms, J. B. (2018). LION/web: a web-based ontology enrichment tool for lipidomic data analysis. bioRxiv. https://doi.org/10.1101/398040
Kong, P., Lehmann, M. J., Helms, J. B., Brouwers, J. F., & Gupta, N. (2018). Lipid analysis of sporozoites reveals exclusive phospholipids, a phylogenetic mosaic of endogenous synthesis, and a host-independent lifestyle. Cell Discovery, 4, [24]. https://doi.org/10.1038/s41421-018-0023-4
Jeucken, A., Bernd Helms, J., & Brouwers, J. F. (2018). Cardiolipin synthases of Escherichia coli have phospholipid class specific phospholipase D activity dependent on endogenous and foreign phospholipids. Biochimica et Biophysica Acta, 1863(10), 1345-1353. https://doi.org/10.1016/j.bbalip.2018.06.017
2017
Wetenschappelijke publicaties
Kruitwagen, H. S., Oosterhoff, L. A., Vernooij, I. G. W. H., Schrall, I. M., van Wolferen, M. E., Bannink, F., Roesch, C., van Uden, L., Molenaar, M. R., Helms, J. B., Grinwis, G. C. M., Verstegen, M. M. A., van der Laan, L. J. W., Huch, M., Geijsen, N., Vries, R. R. G., Clevers, H., Rothuizen, J., Schotanus, B. A., ... Spee, B. (2017). Long-Term Adult Feline Liver Organoid Cultures for Disease Modeling of Hepatic Steatosis. Stem Cell Reports, 8(4), 822-830. https://doi.org/10.1016/j.stemcr.2017.02.015
Molenaar, M. R., Vaandrager, A. B., & Helms, J. B. (2017). Some Lipid Droplets Are More Equal Than Others: Different Metabolic Lipid Droplet Pools in Hepatic Stellate Cells. Lipid Insights, 10, 1-3. https://doi.org/10.1177/1178635317747281
Tuohetahuntila, M., Molenaar, M. R., Spee, B., Brouwers, J. F., Wubbolts, R., Houweling, M., Yan, C., Du, H., VanderVen, B. C., Vaandrager, A. B., & Helms, J. B. (2017). Lysosome-mediated degradation of a distinct pool of lipid droplets during hepatic stellate cell activation. Journal of Biological Chemistry, 292(30), 12436-12448. https://doi.org/10.1074/jbc.M117.778472
Kong, P., Ufermann, C-M., Zimmermann, D. L. M., Yin, Q., Suo, X., Helms, J. B., Brouwers, J. F., & Gupta, N. (2017). Two phylogenetically and compartmentally distinct CDP-diacylglycerol synthases cooperate for lipid biogenesis in Toxoplasma gondii. Journal of Biological Chemistry, 292(17), 7145-7159. https://doi.org/10.1074/jbc.M116.765487
Van den Bossche, L., Schoonenberg, V. A. C., Burgener, I. A., Penning, L. C., Schrall, I. M., Kruitwagen, H. S., van Wolferen, M. E., Grinwis, G. C. M., Kummeling, A., Rothuizen, J., van Velzen, J. F., Stathonikos, N., Molenaar, M. R., Helms, B. J., Brouwers, J. F. H. M., Spee, B., & van Steenbeek, F. G. (2017). Aberrant hepatic lipid storage and metabolism in canine portosystemic shunts. PLoS One, 12(10), [e0186491]. https://doi.org/10.1371/journal.pone.0186491
Ajat, M., Molenaar, M., Brouwers, J. F. H. M., Vaandrager, A. B., Houweling, M., & Helms, J. B. (2017). Hepatic stellate cells retain the capacity to synthesize retinyl esters and to store neutral lipids in small lipid droplets in the absence of LRAT. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1862(2), 176-187. https://doi.org/10.1016/j.bbalip.2016.10.013
2016
Wetenschappelijke publicaties
Olrichs, N. K., & Helms, J. B. (2016). Novel insights into the function of the conserved domain of the CAP superfamily of proteins. AIMS Biophysics, 3(2), 232-246. https://doi.org/10.3934/biophy.2016.2.232
Maric-Biresev, J., Hunn, J. P., Krut, O., Helms, J. B., Martens, S., & Howard, J. C. (2016). Loss of the interferon-γ-inducible regulatory immunity-related GTPase (IRG), Irgm1, causes activation of effector IRG proteins on lysosomes, damaging lysosomal function and predicting the dramatic susceptibility of Irgm1-deficient mice to infection. BMC Biology, 14, [14:33]. https://doi.org/10.1186/s12915-016-0255-4
Maidina, T., Molenaar, M. R., Spee, B., Brouwers, J. F., Houweling, M., Vaandrager, A. B., & Helms, J. B. (2016). ATGL and DGAT1 are involved in the turnover of newly synthesized triacylglycerols in hepatic stellate cells. Journal of Lipid Research, 57, 1162-1174. https://doi.org/10.1194/jlr.M066415
2015
Wetenschappelijke publicaties
Arroyo-Olarte, R. D., Brouwers, J. F., Kuchipudi, A., Helms, J. B., Biswas, A., Dunay, I. R., Lucius, R., & Gupta, N. (2015). Phosphatidylthreonine and Lipid-Mediated Control of Parasite Virulence. PloS Biology [E], 13(11), [e1002288]. https://doi.org/10.1371/journal.pbio.1002288
van Gestel, R. A., Brouwers, J. F., Ultee, A., Helms, J. B., & Gadella, B. M. (2015). Ultrastructure and lipid composition of detergent-resistant membranes derived from mammalian sperm and two types of epithelial cells. Cell and Tissue Research, 363(1), 129-145. https://doi.org/10.1007/s00441-015-2272-y
Brachet, A., Norwood, S., Brouwers, J. F., Palomer, E., Helms, J. B., Dotti, C. G., & Esteban, J. A. (2015). LTP-triggered cholesterol redistribution activates Cdc42 and drives AMPA receptor synaptic delivery. Journal of Cell Biology, 208(6), 791-806. https://doi.org/10.1083/jcb.201407122
Helms, B., Kaloyanova, D. V., Strating, J. R. P., van Hellemond, J. J., van der Schaar, H. M., Tielens, A. G. M., van Kuppeveld, F. J. M., & Brouwers, J. F. (2015). Targeting of the hydrophobic metabolome by pathogens. Traffic, 16(5), 439-460. https://doi.org/10.1111/tra.12280
Kaloyanova, D., Vogels, M., van Balkom, B. W. M., & Helms, J. B. (2015). Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle. Methods in Molecular Biology, 1225, 29-45. https://doi.org/10.1007/978-1-4939-1625-2_2
Kaloyanova, D., Helms, B., Vogels, M., & Van Balkom, B. (2015). Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle. In n
Tuohetahuntila, M., Spee, B., Kruitwagen, H. S., Wubbolts, R., Brouwers, J. F., van de Lest, C. H., Molenaar, M. R., Houweling, M., Helms, J. B., & Vaandrager, A. B. (2015). Role of long-chain acyl-CoA synthetase 4 in formation of polyunsaturated lipid species in hepatic stellate cells. Biochimica et Biophysica Acta, 1851(2), 220-30. https://doi.org/10.1016/j.bbalip.2014.12.003
Lolicato, F., Brouwers, J. F., van de Lest, C. H. A., Wubbolts, R., Aardema, H., Priore, P., Roelen, B. A. J., Helms, J. B., & Gadella, B. M. (2015). The Cumulus Cell Layer Protects Bovine Maturing Oocyte Against Fatty Acid-Induced Lipotoxicity. Biology of Reproduction, 92(1), [16]. https://doi.org/10.1095/biolreprod.114.120634
Overige resultaten
Gupta, N., Arroyo-Olarte, R., Lucius, R., Brouwers, J. F. H. M., & Helms, J. B. (2015). Genetically modified coccidian parasites useful as vaccines.
2014
Wetenschappelijke publicaties
Olrichs, N. K., Mahalka, A. K., Kaloyanova, D., Kinnunen, P. K., & Helms, B. (2014). Golgi-Associated plant Pathogenesis Related protein 1 (GAPR-1) forms amyloid-like fibrils by interaction with acidic phospholipids and inhibits Aβ aggregation. Amyloid - Journal of Protein Folding Disorders, 21(2), 88-96. https://doi.org/10.3109/13506129.2014.882304
Boerke, A., van der Lit, J., Lolicato, F., Stout, T. A. E., Helms, J. B., & Gadella, B. M. (2014). Removal of GPI-anchored membrane proteins causes clustering of lipid microdomains in the apical head area of porcine sperm. Theriogenology, 81(4), 613-624. https://doi.org/10.1016/j.theriogenology.2013.11.014
Houthuijzen, J. M., Daenen, L. G. M., Roodhart, J. M. L., Oosterom, I., van Jaarsveld, M. T. M., Govaert, K. M., Smith, M. E., Sadatmand, S. J., Rosing, H., Kruse, F., Helms, B. J., van Rooijen, N., Beijnen, J. H., Haribabu, B., van de Lest, C. H. A., & Voest, E. E. (2014). Lysophospholipids secreted by splenic macrophages induce chemotherapy resistance via interference with the DNA damage response. Nature Communications [E], 5, [5275]. https://doi.org/10.1038/ncomms6275
2013
Wetenschappelijke publicaties
Boerke, A., Brouwers, J. F., Olkkonen, V. M., van de Lest, C. H. A., Sostaric, E., Schoevers, E. J., Helms, J. B., & Gadella, B. M. (2013). Involvement of Bicarbonate-Induced Radical Signaling in Oxysterol Formation and Sterol Depletion of Capacitating Mammalian Sperm During In Vitro Fertilization. Biology of Reproduction, 88(1), 1-18. [21]. https://doi.org/10.1095/biolreprod.112.101253
Shoji-Kawata, S., Sumpter Jr, R., Leveno, M., Campbell, G. R., Zou, Z., Kinch, L., Wilkins, A. D., Sun, Q., Pallauf, K., MacDuff, D., Huerta, C., Virgin, H. W., Helms, J. B., Eerland, R., Tooze, S. A., Xavier, R., Lenschow, D. J., Yamamoto, A., King, D., ... Levine, B. (2013). Identification of a candidate therapeutic autophagy-inducing peptide. Nature, 494, 201-206. https://doi.org/10.1038/nature11866
Aardema, H., Lolicato, F., van de Lest, C. H. A., Brouwers, J. F., Vaandrager, A. B., van Tol, H. T. A., Roelen, B. A. J., Vos, P. L. A. M., Helms, J. B., & Gadella, B. M. (2013). Bovine cumulus cells protect maturing oocytes from increased fatty acid levels by massive intracellular lipid storage. Biology of Reproduction, 88(6), 1-15. https://doi.org/10.1095/biolreprod.112.106062
Karasawa, A., Swier, L. J. Y. M., Stuart, M. C. A., Brouwers, J. F. H. M., Helms, J. B., & Poolman, B. (2013). Physicochemical Factors Controlling the Activity and Energy Coupling of an Ionic Strength-gated ATP-binding Cassette (ABC) Transporter. Journal of Biological Chemistry, Oct 11(288 (41)), 29862-29871. https://doi.org/10.1074/jbc.M113.499327
Brouwers, J. F. H. M., Aalberts, M., Jansen, J. W. A., van Niel, G., Wauben, M. H. M., Stout, T. A. E., Helms, J. B., & Stoorvogel, W. (2013). Distinct lipid compositions of two types of human prostasomes. Proteomics, 13, 1660-1666.
Populariserende publicaties
Helms, J. B. (2013). Klein eiwit valt virussen en Alzheimer aan. De Volkskrant, 20.
Overige resultaten
Helms, J. B. (Author). (2013). Nieuwe stof beschermt tegen virussen en bacteriën, nieuws, interview and video. Web publication/site, University Utrecht, The Netherlands. http://www.uu.nl/university/research/NL/strategische-themas/lifesciences/nieuws/Paginas/httppers-uu-nlnieuwe-stof-beschermt-tegen-virussen-en-bacterien.aspx
Helms, J. B. (Author). (2013). Kennisbits Utrecht University website. Web publication/site, Valorisation Centre, University Utrecht. http://kennisbits.nl/mensen/bernd-helms
2012
Wetenschappelijke publicaties
Cebollero, E., van der Vaart, A., Zhao, M., Rieter, E. M., Klionsky, D. J., Helms, J. B., & Reggiori, F. M. (2012). Phosphatidylinositol-3-Phosphate Clearance Plays a Key Role in Autophagosome Completion. Current Biology, 22(17), 1545-1553. http://www.ncbi.nlm.nih.gov/pubmed/22771041
Sodero, A. O., Vriens, J., Ghosh, D., Stegner, D., Brachet, A., Pallotto, M., Sassoe-Pognetto, M., Brouwers, J. F. H. M., Helms, J. B., Nieswandt, B., Voets, T., & Dotti, C. G. (2012). Cholesterol loss during glutamate-mediated excitotoxicity. EMBO Journal, 31, 1764-1773.
Testerink, N., Md Ajat, M. M., Houweling, M., Brouwers, J. F. H. M., Pully, V. V., van Manen, H. W. J., Otto, C., Helms, J. B., & Vaandrager, A. B. (2012). Replacement of Retinyl Esters by Polyunsaturated Triacylglycerol Species in Lipid Droplets of HepaticStellate Cells during Activation. PLoS One, 7(4), 10.1371/journal.pone.0034945. https://doi.org/10.1371/journal.pone.0034945
van Galen, J., Olrichs, N. K., Schouten, A., Serrano, R. L., Nolte-'t Hoen, E. N. M., Eerland, R., Kaloyanova, D., Gros, P., & Helms, J. B. (2012). Interaction of GAPR-1 with lipid bilayers is regulated by alternative homodimerization. Biochimica et Biophysica Acta, 1818(9), 2175-2183. https://doi.org/10.1016/j.bbamem.2012.04.016
Populariserende publicaties
Helms, J. B. (2012). Smeermiddel van de Cel. C2W Life Sciences 21, 24-25.
2011
Wetenschappelijke publicaties
Brouwers, J. F. H. M., Boerke, A., da Silva, P. F. N., Garcia-Gil, N., van Gestel, R. A., Helms, J. B., van de Lest, C. H. A., & Gadella, B. M. (2011). Mass Spectrometric detection of cholestrol oxidation in bovine sperm. Biology of Reproduction, 85, 128-136.
Aardema, H., Vos, P. L. A. M., Lolicato, F., Roelen, B. A. J., Knijn, H. M., Vaandrager, A. B., Helms, J. B., & Gadella, B. M. (2011). Oleic Acid Prevents Detrimental Effects of Saturated Fatty Acids on Bovine Oocyte. Biology of Reproduction, 85(1), 62-69.
Vogels, M. W., van Balkom, B. W. M., Kaloyanova, D. V., Batenburg, J. J., Heck, A. J. R., Helms, J. B., Rottier, P. J. M., & de Haan, C. A. M. (2011). Identification of host factors involved in coronavirus replication by quantitative proteomics analysis. Proteomics, 11(1), 64-80. https://doi.org/10.1002/pmic.201000309
Vogels, M. W., van Balkom, B. W. M., Heck, A. J. R., de Haan, C. A. M., Rottier, P. J. M., Batenburg, J. J., Kaloyanova, D. V., & Helms, J. B. (2011). Quantitative proteomic identification of host factors involved in the Salmonella syphimuruim infectin cycle. Proteomics, 11, 4477-4491. http://www.ncbi.nlm.nih.gov/pubmed?term=Quantitative%20proteomic%20identification%20of%20host%20factors%20involved%20in%20the%20Salmonella%20syphimuruim%20infectin%20cycle.
2010
Wetenschappelijke publicaties
van der Meer, Y. P. M., van Galen, J., Batenburg, J. J., & Helms, J. B. (2010). Lipids in host–pathogen interactions: Pathogens exploit the complexity of the host cell lipidome. Progress in Lipid Research, 49, 1-26.
van Galen, J., van Balkom, B. W. M., Serrano, R. L., Kaloyanova, D. V., Eerland, R., Stuven, E., & Helms, J. B. (2010). Binding of GAPR-1 to negatively charged phospholipid membranes: unusual binding characteristics to phosphatidylinositol. Molecular Membrane Biology, 27(2-3), 81-91. http://www.ncbi.nlm.nih.gov/pubmed/20095951
Bot, M., Bot, I., Lopez-Vales, R., van de Lest, C. H. A., Saulnier-Blache, J. S., Helms, J. B., David, S., van Berkel, T. J. C., & Biessen, E. A. L. (2010). Atherosclerotic lesion progression changes lysophosphatidic acid homeostasis to favor ist accumulation. American Journal of Pathology, 176(6), 3073-3084.
2009
Wetenschappelijke publicaties
van de Wetering, K., Feddema, W., Helms, J. B., Brouwers, J. F. H. M., & Borst, P. (2009). Targeted metabolomics iIdentifies glucuronides of dietary phytoestrogens as a major class of MRP3 substrates in vivo. Gastroenterology, 137, 1725-1735.
https://dspace.library.uu.nl/bitstream/handle/1874/39578/Targeted%2520metabolomics%2520identifies%2520glucuronides%2520of%2520dietary%2520phytoestrogens%2520as%2520a%2520major%2520class%2520of%2520MRP3%2520substrates%2520in%2520vivo.pdf?sequence=1
https://dspace.library.uu.nl/bitstream/handle/1874/39578/Targeted%2520metabolomics%2520identifies%2520glucuronides%2520of%2520dietary%2520phytoestrogens%2520as%2520a%2520major%2520class%2520of%2520MRP3%2520substrates%2520in%2520vivo.pdf?sequence=1
Verheijen, M. H. G., Camargo, N., Verdier, V., Nadra, K., de Preux, A., Medard, J., Luoma, A., Crowther, M., Inouye, H., Shimano, H., Chen, S., Brouwers, J. F. H. M., Helms, J. B., Feltri, M. L., Wrabetz, L., Kirschner, D., Chrast, R., & Smit, A. B. (2009). SREBP cleavage activation protein (SCAP) is required for timely synthesis of a Schwann cell myelin membrane. Proceedings of the National Academy of Sciences of the United States of America, 106, 21383-21388.
Testerink, N., van der Sanden, M. H. M., Houweling, M., Helms, J. B., & Vaandrager, A. B. (2009). Depletion of phosphatidylcholine affects ER morphology and protein traffic at the Golgi complex. Journal of Lipid Research, 50, 2182-2206.
2008
Wetenschappelijke publicaties
van Meer, G., Spener, F., Leeflang, B. R., Beisiegel, U., Bougnoux, P., Goñi, F. M., Griffiths, W., Hartmann, T., Helms, J. B., Hoekstra, D., Julià-Sapé, M., Larijani, B., Moschetta, A., Mouritsen, O. G., Danilo Norata, G., Payrastre, B., Record, M., Schmitz, G., Simons, K., ... Wanders, R. J. A. (2008). Structure Medicine II: The Importance of Lipidomics for Health and Disease. In European Science Foundation Policy Breefing European Science Foundation Policy Briefing. http://www.esf.org/fileadmin/Public_documents/Publications/SPB31_StructuralMedicineII.pdf
Overige resultaten
Burger, K. N. J., & Helms, J. B. (2008, May 1). EU Seventh Framework Programme (FP7) "Lipid droplets as dynamic organelles of fata deposition and release: translational research towards human disease" 27 partners: 22 research institutes including Utrecht University and 4 SMEs; total budget 11.6 MEuro].
Vogels, M. W., Kaloyanova, D. V., van Balkom, B. W. M., Batenburg, J. J., Helms, J. B., Rottier, P. J. M., & de Haan, C. A. M. (2008). Interactions of intracellular pathogens with the Golgi apparatus.. Poster session presented at The XIth International Symposium on Nidovirus, St. Catherine's College, Oxford, United Kingdom.
2007
Wetenschappelijke publicaties
Helms, J. B. (2007). Structural Medicine II: the importance of Lipidomics for health and disease. European Science Foundation Policy Briefing.
Bleijerveld, O. B., Brouwers, J. F. H. M., Vaandrager, A. B., Helms, J. B., & Houweling, M. (2007). The CDP-ethanolamine pathway and phosphatidylserine decarboxylation generate different phosphatidylethanolamine molecular species. Journal of Biological Chemistry, 282, 28362-28372.
https://dspace.library.uu.nl/bitstream/handle/1874/26169/43_CDP_Bleijerveld.pdf?sequence=1
https://dspace.library.uu.nl/bitstream/handle/1874/26169/43_CDP_Bleijerveld.pdf?sequence=1
Huitema, L. F. A., van Weeren, R., van Balkom, B. W. M., Visser, T., van de Lest, C. H. A., Barneveld, A., Helms, J. B., & Vaandrager, A. B. (2007). Soluble factors released by ATDC5 cells affect the formation of calcium phosphate crystals. Biochimica et Biophysica Acta-Proteins Proteomics, 1774, 1108-1117.
Li, X., Kaloyanova, D. V., van Eijk, M., Eerland, R., van der Goot, G., Oorschot, V. M. J., Klumperman, J., Lottspeich, F., Starkuviene, V., Wieland, F. T., & Helms, J. B. (2007). Involvement of a Golgi-resident GPI-anchored protein in maintenance of the Golgi structure. Molecular Biology of the Cell, 18(4), 1261-1271.
2006
Wetenschappelijke publicaties
Helms, J. B. (2006). Host-Pathogen interactions: Lipids grease the way. European Journal of Lipid Science and Technology, 108, 895-897.
Helms, J. B., & van Meer, G. (2006). Lipidome and disease. FEBS Letters, 580, 5429.
https://dspace.library.uu.nl/bitstream/handle/1874/20260/6.Helms%25202006%2520pp%25205429.pdf?sequence=1
https://dspace.library.uu.nl/bitstream/handle/1874/20260/6.Helms%25202006%2520pp%25205429.pdf?sequence=1
Huitema, L. F. A., Vaandrager, A. B., van Weeren, P. R., Barneveld, A., Helms, J. B., & van de Lest, C. H. A. (2006). The nitric oxide donor sodium nitroprusside inhibits mineralization in ATDC5 cells. Calcified Tissue International, 78(3), 171-177.
Helms, J. B. (2006). The importance of lipidomics for health and disease. In F. S. G. van Meer (Ed.), Structural Medicine II European Science Foundation.
Huitema, L. F. A., van Weeren, P. R., Barneveld, A., van de Lest, C. H. A., Helms, J. B., & Vaandrager, A. B. (2006). Iron ions derived from the nitric oxide donor sodium nitroprusside inhibit mineralization. European Journal of Pharmacology, 542, 48-53.
2005
Wetenschappelijke publicaties
Kokubo, H., Saido, T. C., Iwata, N., Helms, J. B., Shinohara, R., & Yamaguchi, H. (2005). Part of membrane-bound Abeta exists in rafts within senile plaques in Tg2576 mouse brain. Neurobiology of Aging, 4, 409-418.
van Gestel, R. A., Brewis, I. A., Ashton, P. R., Helms, J. B., Brouwers, J. F. H. M., & Gadella, B. M. (2005). Capacitation-dependent concentration of lipid rafts in the apical ridge head area of porcine sperm cells. Molecular Human Reproduction, 11(8), 583-590.
van Gestel, R. A., Helms, J. B., Brouwers, J. F. H. M., & Gadella, B. M. (2005). Effects of methyl-beta-cyclodextrin-mediated cholesterol depletion in porcine sperm compared to somatic cells. Molecular Reproduction and Development, 72, 386-395.
Kokubo, H., Kayed, R., Glabe, C. G., Saido, T. C., Iwata, N., Helms, J. B., & Yamaguchi, H. (2005). Oligomeric proteins ultrastructurally localize to cell processes, especially to axon terminals with higher density, but not to lipid rafts in Tg2576 mouse brain. Brain Research, 1045, 224-228.
Helms, J. B. (2005). Membrane dynamics and pathogenesis. In NWO Chemische Wetenschappen, jaarboek 2005 (pp. 162-163)
2004
Wetenschappelijke publicaties
Serrano, R. L., Kuhn, A., Hendricks, A., Helms, J. B., Sinning, I., & Groves, M. R. (2004). Structural analysis of the human Golgi-associated plant pathogenesis related protein GAPR-1 implicates dimerization as a regulatory mechanism. Journal of Molecular Biology, 339(1), 173-183.
Helms, J. B., & Zurzolo, C. (2004). Lipids as targeting signals: lipid rafts and intracellular trafficking. Traffic, 5(4), 247-254.
van der Sanden, M. H. M., Meems, M. H., Houweling, M., Helms, J. B., & Vaandrager, A. B. (2004). Induction of CCAAT/Enhancer-binding protein (C/EBP)-homologous protein/growth arrest and DNA damage-inducible protein 153 expression during inhibition of phosphatidylcholine synthesis is mediated via activation of a C/EBP-activating transcription factor-responsive element. Journal of Biological Chemistry, 279(50), 52007-52015.
Groves, M. R., Kuhn, A., Hendricks, A., Radke, S., Serrano, R. L., Helms, J. B., & Sinning, I. (2004). Crystallization of a Golgi-associated PR-1-related protein (GAPR-1) that localizes to lipid-enriched microdomains. Acta crystallographica. Section D, biological crystallography, 60(4), 730-732.
Bleijerveld, O. B., Klein, W., Vaandrager, A. B., Helms, J. B., & Houweling, M. (2004). Control of the CDPethanolamine pathway in mammalian cells: effect of CTP:phosphoethanolamine cytidylyltransferase overexpression and the amount of intracellular diacylglycerol. Biochemical Journal, 379(3), 711-719.
2003
Wetenschappelijke publicaties
Kokubo, H., Helms, J. B., Ohno-Iwashita, Y., Shimada, Y., Horikoshi, Y., & Yamaguchi, H. (2003). Ultrastructural localization of flotillin-1 to cholesterol-rich membrane microdomains, rafts, in rat brain tissue. Brain Research, 965, 83-90.
Stüven, E. P., Porat, A., Shimron, F., Fass, E., Kaloyanova, D. V., Brügger, B., Wieland, F. T., Elazar, Z., & Helms, J. B. (2003). Intra-Golgi protein transport depends on a cholesterol balance in the lipid membrane. Journal of Biological Chemistry, 278, 53112-53122.
da Silva, P. F. N., Brouwers, J. F. H. M., Helms, J. B., Colenbrander, B. A., & Gadella, B. M. (2003). Cholesterol oxidation is a common phenomenon in bovine sperm cells. Reproduction, 30, 87-88.
Aniento, F., Helms, J. B., & Memon, A. R. (2003). How to make a vesicle: coat protein-membrane interactions. In Robinson, D.G. (Ed.), Annual Plant Reviews 9, The Golgi apparatus and the plant secretory pathway. Academic Press Inc..
2002
Wetenschappelijke publicaties
Eberle, H. B., Serrano, R. L., Füllekrug, J., Schlosser, A., Lehmann, W. D., Lottspeich, F., Kaloyanova, D., Wieland, F. T., & Helms, J. B. (2002). Identification and characterization of a novel human plant pathogenesis-related protein that localizes to lipid-enriched microdomains in the Golgi complex. Journal of Cell Science, 115(Pt 4), 827-38.
2001
Wetenschappelijke publicaties
Gommel, D. U., Memon, A. R., Heiss, A., Lottspeich, F., Pfannstiel, J., Lechner, J., Reinhard, C., Helms, J. B., Nickel, W., & Wieland, F. T. (2001). Recruitment to Golgi membranes of ADP-ribosylation factor 1 is mediated by the cytoplasmic domain of p23. EMBO Journal, 20(23), 6751-60. https://doi.org/10.1093/emboj/20.23.6751
Gkantiragas, I., Brügger, B., Stüven, E., Kaloyanova, D., Li, X. Y., Löhr, K., Lottspeich, F., Wieland, F. T., & Helms, J. B. (2001). Sphingomyelin-enriched microdomains at the Golgi complex. Molecular Biology of the Cell, 12(6), 1819-33.
2000
Wetenschappelijke publicaties
Brügger, B., Sandhoff, R., Wegehingel, S., Gorgas, K., Malsam, J., Helms, J. B., Lehmann, W. D., Nickel, W., & Wieland, F. T. (2000). Evidence for segregation of sphingomyelin and cholesterol during formation of COPI-coated vesicles. Journal of Cell Biology, 151(3), 507-18.
Fischer, K. D., Helms, J. B., Zhao, L., & Wieland, F. T. (2000). Site-specific photocrosslinking to probe interactions of Arf1 with proteins involved in budding of COPI vesicles. Methods, 20(4), 455-64. https://doi.org/10.1006/meth.2000.0958
1999
Wetenschappelijke publicaties
Zhao, L., Helms, J. B., Brunner, J., & Wieland, F. T. (1999). GTP-dependent binding of ADP-ribosylation factor to coatomer in close proximity to the binding site for dilysine retrieval motifs and p23. Journal of Biological Chemistry, 274(20), 14198-203.
Reinhard, C., Harter, C., Bremser, M., Brügger, B., Sohn, K., Helms, J. B., & Wieland, F. (1999). Receptor-induced polymerization of coatomer. Proceedings of the National Academy of Sciences of the United States of America, 96(4), 1224-8.
Gommel, D., Orci, L., Emig, E. M., Hannah, M. J., Ravazzola, M., Nickel, W., Helms, J. B., Wieland, F. T., & Sohn, K. (1999). p24 and p23, the major transmembrane proteins of COPI-coated transport vesicles, form hetero-oligomeric complexes and cycle between the organelles of the early secretory pathway. FEBS Letters, 447(2-3), 179-85.
1998
Wetenschappelijke publicaties
Nickel, W., Malsam, J., Gorgas, K., Ravazzola, M., Jenne, N., Helms, J. B., & Wieland, F. T. (1998). Uptake by COPI-coated vesicles of both anterograde and retrograde cargo is inhibited by GTPgammaS in vitro. Journal of Cell Science, 111 ( Pt 20), 3081-90.
Helms, J. B., Helms-Brons, D., Brügger, B., Gkantiragas, I., Eberle, H., Nickel, W., Nürnberg, B., Gerdes, H. H., & Wieland, F. T. (1998). A putative heterotrimeric G protein inhibits the fusion of COPI-coated vesicles. Segregation of heterotrimeric G proteins from COPI-coated vesicles. Journal of Biological Chemistry, 273(24), 15203-8.
1997
Wetenschappelijke publicaties
Galas, M. C., Helms, J. B., Vitale, N., Thiersé, D., Aunis, D., & Bader, M. F. (1997). Regulated exocytosis in chromaffin cells. A potential role for a secretory granule-associated ARF6 protein. Journal of Biological Chemistry, 272(5), 2788-93.
Zhao, L., Helms, J. B., Brügger, B., Harter, C., Martoglio, B., Graf, R., Brunner, J., & Wieland, F. T. (1997). Direct and GTP-dependent interaction of ADP ribosylation factor 1 with coatomer subunit beta. Proceedings of the National Academy of Sciences of the United States of America, 94(9), 4418-23.
1996
Wetenschappelijke publicaties
Nickel, W., Helms, J. B., Kneusel, R. E., & Wieland, F. T. (1996). Forskolin stimulates detoxification of brefeldin A. Journal of Biological Chemistry, 271(27), 15870-3.
Sohn, K., Orci, L., Ravazzola, M., Amherdt, M., Bremser, M., Lottspeich, F., Fiedler, K., Helms, J. B., & Wieland, F. T. (1996). A major transmembrane protein of Golgi-derived COPI-coated vesicles involved in coatomer binding. Journal of Cell Biology, 135(5), 1239-48.
1995
Wetenschappelijke publicaties
Helms, J. B. (1995). Role of heterotrimeric GTP binding proteins in vesicular protein transport: indications for both classical and alternative G protein cycles. FEBS Letters, 369(1), 84-8.
1993
Wetenschappelijke publicaties
Tanigawa, G., Orci, L., Amherdt, M., Ravazzola, M., Helms, J. B., & Rothman, J. E. (1993). Hydrolysis of bound GTP by ARF protein triggers uncoating of Golgi-derived COP-coated vesicles. Journal of Cell Biology, 123(6 Pt 1), 1365-71.
Helms, J. B., Palmer, D. J., & Rothman, J. E. (1993). Two distinct populations of ARF bound to Golgi membranes. Journal of Cell Biology, 121(4), 751-60.
Palmer, D. J., Helms, J. B., Beckers, C. J., Orci, L., & Rothman, J. E. (1993). Binding of coatomer to Golgi membranes requires ADP-ribosylation factor. Journal of Biological Chemistry, 268(16), 12083-9.
1992
Wetenschappelijke publicaties
Helms, J. B., & Rothman, J. E. (1992). Inhibition by brefeldin A of a Golgi membrane enzyme that catalyses exchange of guanine nucleotide bound to ARF. Nature, 360(6402), 352-4. https://doi.org/10.1038/360352a0
1991
Wetenschappelijke publicaties
Helms, J. B., de Vries, K. J., & Wirtz, K. W. (1991). Synthesis of phosphatidylinositol 4,5-bisphosphate in the endoplasmic reticulum of Chinese hamster ovary cells. Journal of Biological Chemistry, 266(32), 21368-74.
1990
Wetenschappelijke publicaties
Capitani, S., Helms, B., Mazzoni, M., Previati, M., Bertagnolo, V., Wirtz, K. W., & Manzoli, F. A. (1990). Uptake and phosphorylation of phosphatidylinositol by rat liver nuclei. Role of phosphatidylinositol transfer protein. Biochimica et Biophysica Acta, 1044(2), 193-200.
Helms, J. B., Karrenbauer, A., Wirtz, K. W., Rothman, J. E., & Wieland, F. T. (1990). Reconstitution of steps in the constitutive secretory pathway in permeabilized cells. Secretion of glycosylated tripeptide and truncated sphingomyelin. Journal of Biological Chemistry, 265(32), 20027-32.
1987
Wetenschappelijke publicaties
van Amerongen, A., Helms, J. B., van Noort, M., Rommerts, F. F. G., & Wirtz, K. W. A. (1987). Evidence for a link between peroxisomes and the non-specific lipid transfer protein (SCP2) in rat and human liver. In H. D. Fakimi, & H. Sies (Eds.), Peroxisomes in biology and medicine Springer-Verlag Berlin Heidelberg.
van Amerongen, A., Helms, J. B., van der Krift, T. P., Schutgens, R. B., & Wirtz, K. W. (1987). Purification of nonspecific lipid transfer protein (sterol carrier protein 2) from human liver and its deficiency in livers from patients with cerebro-hepato-renal (Zellweger) syndrome. Biochimica et Biophysica Acta, 919(2), 149-55.