Prof. dr. E.J. (Eefjan) Breukink

Prof. dr. E.J. (Eefjan) Breukink

Hoogleraar
Membrane Biochemistry & Biophysics
030 253 3523
e.j.breukink@uu.nl

Publicaties

2023

Wetenschappelijke publicaties

Shukla, R., Peoples, A. J., Ludwig, K. C., Maity, S., Derks, M. G. N., de Benedetti, S., Krueger, A. M., Vermeulen, B. J. A., Lavore, F., Honorato, R. V., Grein, F., Bonvin, A., Kubitscheck, U., Breukink, E., Achorn, C., Nitti, A., Schwalen, C. J., Spoering, A. L., Ling, L. L., ... Weingarth, M. (2023). A new antibiotic from an uncultured bacterium binds to an immutable target. bioRxiv. https://doi.org/10.1101/2023.05.15.540765
Wang, X., van Beekveld, R. A. M., Xu, Y., Parmar, A., Das, S., Singh, I., & Breukink, E. (2023). Analyzing mechanisms of action of antimicrobial peptides on bacterial membranes requires multiple complimentary assays and different bacterial strains. Biochimica et Biophysica Acta - Biomembranes, 1865(6), [184160]. https://doi.org/10.1016/j.bbamem.2023.184160
https://dspace.library.uu.nl/bitstream/handle/1874/429607/1_s2.0_S0005273623000421_main.pdf?sequence=1

2022

Wetenschappelijke publicaties

van Beekveld, R. A. M., Derks, M. G. N., Kumar, R., Smid, L., Maass, T., Medeiros-Silva, J., Breukink, E., & Weingarth, M. (2022). Specific Lipid Studies in Complex Membranes by Solid-State NMR Spectroscopy. Chemistry - A European Journal, 28(70), [e202202472]. https://doi.org/10.1002/chem.202202472
Xu, Y., Hernández-Rocamora, V. M., Lorent, J. H., Cox, R., Wang, X., Bao, X., Stel, M., Vos, G., van den Bos, R. M., Pieters, R. J., Gray, J., Vollmer, W., & Breukink, E. (2022). Metabolic labeling of the bacterial peptidoglycan by functionalized glucosamine. iScience, 25(8), [104753]. https://doi.org/10.1016/j.isci.2022.104753
Shukla, R., Lavore, F., Maity, S., Derks, M. G. N., Jones, C. R., Vermeulen, B. J. A., Melcrová, A., Morris, M. A., Becker, L. M., Wang, X., Kumar, R., Medeiros-Silva, J., van Beekveld, R. A. M., Bonvin, A. M. J. J., Lorent, J. H., Lelli, M., Nowick, J. S., MacGillavry, H. D., Peoples, A. J., ... Weingarth, M. (2022). Teixobactin kills bacteria by a two-pronged attack on the cell envelope. Nature, 608(7922), 390-396. https://doi.org/10.1038/s41586-022-05019-y

2021

Wetenschappelijke publicaties

Li, Y., Boes, A., Cui, Y., Zhao, S., Liao, Q., Gong, H., Breukink, E., Lutkenhaus, J., Terrak, M., & Du, S. (2021). Identification of the potential active site of the septal peptidoglycan polymerase FtsW. PLoS Genetics, 18(1), 1-25. [e1009993]. https://doi.org/10.1371/journal.pgen.1009993
Zhao, X., Wang, X., Shukla, R., Kumar, R., Weingarth, M., Breukink, E., & Kuipers, O. P. (2021). Brevibacillin 2V Exerts Its Bactericidal Activity via Binding to Lipid II and Permeabilizing Cellular Membranes. Frontiers in Microbiology, 12, 1-13. [694847]. https://doi.org/10.3389/fmicb.2021.694847
Zhao, X., Wang, X., Shukla, R., Kumar, R., Weingarth, M., Breukink, E., & Kuipers, O. P. (2021). Brevibacillin 2V, a Novel Antimicrobial Lipopeptide With an Exceptionally Low Hemolytic Activity. Frontiers in Microbiology, 12, 1-11. [693725]. https://doi.org/10.3389/fmicb.2021.693725
van Eijk, M., van Dijk, A., van der Ent, C. K., Arets, H. G. M., Breukink, E., van Os, N., Adrichem, R., van der Water, S., Gomez, R. L., Kristensen, M., Hessing, M., Jekhmane, S., Weingarth, M., Veldhuizen, R. A. W., Veldhuizen, E. J. A., & Haagsman, H. P. (2021). PepBiotics, novel cathelicidin-inspired antimicrobials to fight pulmonary bacterial infections. Biochimica et Biophysica Acta - General Subjects, 1865(9), [129951]. https://doi.org/10.1016/j.bbagen.2021.129951
Sassine, J., Pazos, M., Breukink, E., & Vollmer, W. (2021). Lytic transglycosylase MltG cleaves in nascent peptidoglycan and produces short glycan strands. The Cell Surface, 7, 1-9. [100053]. https://doi.org/10.1016/j.tcsw.2021.100053
Hernández-Rocamora, V. M., Baranova, N., Peters, K., Breukink, E., Loose, M., & Vollmer, W. (2021). Real-time monitoring of peptidoglycan synthesis by membrane-reconstituted penicillin-binding proteins. eLife, 10, 1-79. [e61525]. https://doi.org/10.7554/eLife.61525

2020

Wetenschappelijke publicaties

Boes, A., Kerff, F., Herman, R., Touzé, T., Breukink, E., & Terrak, M. (2020). The bacterial cell division protein fragment E FtsN binds to and activates the major peptidoglycan synthase PBP1b. Journal of Biological Chemistry, 295(52), 18256-18265. https://doi.org/10.1074/jbc.ra120.015951
Pazos, M., Peters, K., Boes, A., Safaei, Y., Kenward, C., Caveney, N. A., Laguri, C., Breukink, E., Strynadka, N. C. J., Simorre, J. P., Terrak, M., & Vollmer, W. (2020). SPOR Proteins Are Required for Functionality of Class A Penicillin-Binding Proteins in Escherichia coli. mBio, 11(6). https://doi.org/10.1128/mBio.02796-20
Boes, A., Brunel, J. M., Derouaux, A., Kerff, F., Bouhss, A., Touze, T., Breukink, E., & Terrak, M. (2020). Squalamine and aminosterol mimics inhibit the peptidoglycan glycosyltransferase activity of pbp1b. Antibiotics, 9(7), [373]. https://doi.org/10.3390/antibiotics9070373
Shukla, R., Medeiros-Silva, J., Parmar, A., Vermeulen, B. J. A., Das, S., Paioni, A. L., Jekhmane, S., Lorent, J., Bonvin, A. M. J. J., Baldus, M., Lelli, M., Veldhuizen, E. J. A., Breukink, E., Singh, I., & Weingarth, M. (2020). Mode of action of teixobactins in cellular membranes. Nature Communications, 11(1), 2848. https://doi.org/10.1038/s41467-020-16600-2
Banzhaf, M., Yau, H. C., Verheul, J., Lodge, A., Kritikos, G., Mateus, A., Cordier, B., Hov, A. K., Stein, F., Wartel, M., Pazos, M., Solovyova, A. S., Breukink, E., van Teeffelen, S., Savitski, M. M., den Blaauwen, T., Typas, A., & Vollmer, W. (2020). Outer membrane lipoprotein NlpI scaffolds peptidoglycan hydrolases within multi-enzyme complexes in Escherichia coli. EMBO Journal, 39(5), [e102246]. https://doi.org/10.15252/embj.2019102246
Wang, X., Gu, Q., & Breukink, E. (2020). Non-lipid II targeting lantibiotics. Biochimica et Biophysica Acta - Biomembranes, 1862(8), [183244]. https://doi.org/10.1016/j.bbamem.2020.183244
Zhao, X., Yin, Z., Breukink, E., Moll, G. N., & Kuipers, O. P. (2020). An Engineered Double Lipid II Binding Motifs-Containing Lantibiotic Displays Potent and Selective Antimicrobial Activity against Enterococcus faecium. Antimicrobial Agents and Chemotherapy, 64(6), [e02050-19]. https://doi.org/10.1128/AAC.02050-19
Boes, A., Olatunji, S., Mohammadi, T., Breukink, E., & Terrak, M. (2020). Fluorescence anisotropy assays for high throughput screening of compounds binding to lipid II, PBP1b, FtsW and MurJ. Scientific Reports, 10(1), [6280]. https://doi.org/10.1038/s41598-020-63380-2
Caveney, N. A., Egan, A. J. F., Ayala, I., Laguri, C., Robb, C. S., Breukink, E., Vollmer, W., Strynadka, N. C. J., & Simorre, J-P. (2020). Structure of the Peptidoglycan Synthase Activator LpoP in Pseudomonas aeruginosa. Structure with Folding & design, 28(6), 643-650.e5. https://doi.org/10.1016/j.str.2020.03.012

2019

Wetenschappelijke publicaties

Kuru, E., Radkov, A., Meng, X., Egan, A., Alvarez, L., Dowson, A., Booher, G., Breukink, E., Roper, D. I., Cava, F., Vollmer, W., Brun, Y., & VanNieuwenhze, M. S. (2019). Mechanisms of incorporation for D-amino acid probes that target peptidoglycan biosynthesis. ACS Chemical Biology, 14(12), 2745-2756. https://doi.org/10.1021/acschembio.9b00664
Medeiros-Silva, J., Jekhmane, S., Breukink, E., & Weingarth, M. (2019). High-resolution NMR studies of antibiotics in cell membranes. 253. https://doi.org/10.1002/2211-5463.12675
Mueller, E. A., Egan, A. J., Breukink, E., Vollmer, W., & Levin, P. A. (2019). Plasticity of Escherichia coli cell wall metabolism promotes fitness and antibiotic resistance across environmental conditions. eLife, 2019(8), [e40754]. https://doi.org/10.7554/eLife.40754
Pazos, M., Palacios, P., Vicente, M., Breukink, E., Peters, K., Casanova, M., Vollmer, W., & VanNieuwenhze, M. (2019). Publisher Correction: Z-ring membrane anchors associate with cell wall synthases to initiate bacterial cell division. Nature Communications, 10(1). https://doi.org/10.1038/s41467-019-08410-y
Morè, N., Martorana, A. M., Biboy, J., Otten, C., Winkle, M., Serrano, C. K. G., Montón Silva, A., Atkinson, L., Yau, H., Breukink, E., den Blaauwen, T., Vollmer, W., & Polissi, A. (2019). Peptidoglycan Remodeling Enables Escherichia coli To Survive Severe Outer Membrane Assembly Defect. mBio, 10(1), [e02729-18]. https://doi.org/10.1128/mBio.02729-18
Medeiros-Silva, J., Jekhmane, S., Breukink, E., & Weingarth, M. (2019). Towards the native binding modes of Lipid II targeting antibiotics. ChemBioChem, 20(14), 1731-1738. https://doi.org/10.1002/cbic.201800796
Boes, A., Olatunji, S., Breukink, E., & Terrak, M. (2019). Regulation of the Peptidoglycan Polymerase Activity of PBP1b by Antagonist Actions of the Core Divisome Proteins FtsBLQ and FtsN. mBio, 10(1), [e01912-18]. https://doi.org/10.1128/mBio.01912-18

2018

Wetenschappelijke publicaties

Liu, Y., Moura, E. C. C. M., Dörr, J. M., Scheidelaar, S., Heger, M., Egmond, M. R., Killian, J. A., Mohammadi, T., & Breukink, E. (2018). Correction: Bacillus subtilis MraY in detergent-free system of nanodiscs wrapped by styrene-maleic acid copolymers. PLoS One, 13(12), e0210135. https://doi.org/10.1371/journal.pone.0210135
Pazos, M., Peters, K., Casanova, M., Palacios, P., VanNieuwenhze, M., Breukink, E., Vicente, M., & Vollmer, W. (2018). Z-ring membrane anchors associate with cell wall synthases to initiate bacterial cell division. Nature Communications, 9(1), [5090]. https://doi.org/10.1038/s41467-018-07559-2
Liu, Y., Moura, E. C. C. M., Dörr, J. M., Scheidelaar, S., Heger, M., Egmond, M. R., Killian, J. A., Mohammadi, T., & Breukink, E. (2018). Bacillus subtilis MraY in detergent-free system of nanodiscs wrapped by styrene-maleic acid copolymers. PLoS One, 13(11), [e0206692]. https://doi.org/10.1371/journal.pone.0206692
Medeiros-Silva, J., Jekhmane, S., Paioni, A. L., Gawarecka, K., Baldus, M., Swiezewska, E., Breukink, E., & Weingarth, M. (2018). High-resolution NMR studies of antibiotics in cellular membranes. Nature Communications, 9(1), [3963]. https://doi.org/10.1038/s41467-018-06314-x
Egan, A. J. F., Maya-Martinez, R., Ayala, I., Bougault, C. M., Banzhaf, M., Breukink, E., Vollmer, W., & Simorre, J-P. (2018). Induced conformational changes activate the peptidoglycan synthase PBP1B. Molecular Microbiology, 110(3), 335-356. https://doi.org/10.1111/mmi.14082
Hernández-Rocamora, V. M., Otten, C. F., Radkov, A., Simorre, J-P., Breukink, E., VanNieuwenhze, M., & Vollmer, W. (2018). Coupling of polymerase and carrier lipid phosphatase prevents product inhibition in peptidoglycan synthesis. Cell surface (Amsterdam), 2, 1-13. https://doi.org/10.1016/j.tcsw.2018.04.002
Jiang, H., Tang, X., Zhou, Q., Zou, J., Li, P., Breukink, E., & Gu, Q. (2018). Plantaricin NC8 from Lactobacillus plantarum causes cell membrane disruption to Micrococcus luteus without targeting lipid II. Applied Microbiology and Biotechnology, 102(17), 7465-7473. https://doi.org/10.1007/s00253-018-9182-3
Silva, A. M., Otten, C., Biboy, J., Breukink, E., Van Nieuwenhze, M., Vollmer, W., & den Blaauwen, T. (2018). The fluorescent D-Amino Acid NADA as a tool to study the conditional activity of transpeptidases in Escherichia coli. Frontiers in Microbiology, 9(SEP). https://doi.org/10.3389/fmicb.2018.02101
Morlot, C., Straume, D., Peters, K., Hegnar, O. A., Simon, N., Villard, A-M., Contreras-Martel, C., Leisico, F., Breukink, E., Gravier-Pelletier, C., Le Corre, L., Vollmer, W., Pietrancosta, N., Håvarstein, L. S., & Zapun, A. (2018). Structure of the essential peptidoglycan amidotransferase MurT/GatD complex from Streptococcus pneumoniae. Nature Communications, 9(1), [3180]. https://doi.org/10.1038/s41467-018-05602-w

2017

Wetenschappelijke publicaties

Parmar, A., Iyer, A., Prior, S. H., Lloyd, D. G., Leng Goh, E. T., Vincent, C. S., Palmai-Pallag, T., Bachrati, C. Z., Breukink, E., Madder, A., Lakshminarayanan, R., Taylor, E. J., & Singh, I. (2017). Teixobactin analogues reveal enduracididine to be non-essential for highly potent antibacterial activity and lipid II binding. Chemical Science, 8(12), 8183-8192. https://doi.org/10.1039/c7sc03241b
Leclercq, S., Derouaux, A., Olatunji, S., Fraipont, C., Egan, A. J. F., Vollmer, W., Breukink, E., & Terrak, M. (2017). Interplay between Penicillin-binding proteins and SEDS proteins promotes bacterial cell wall synthesis. Scientific Reports, 7, [43306]. https://doi.org/10.1038/srep43306
Parmar, A., Prior, S. H., Iyer, A., Vincent, C. S., Van Lysebetten, D., Breukink, E., Madder, A., Taylor, E. J., & Singh, I. (2017). Defining the molecular structure of teixobactin analogues and understanding their role in antibacterial activities. Chemical Communications, 53(12), 2016-2019. https://doi.org/10.1039/c6cc09490b
't Hart, P., Wood, T. M., Hajmohammadebrahimtehrani, K., van Harten, R. M., Sleszynska, M. K., Breukink, E. J., & Martin, N. I. (2017). De novo identification of lipid II binding lipopeptides with antibacterial activity against vancomycin-resistant bacteria. Chemical Science, 8, 7991-7997. https://doi.org/10.1039/C7SC03413J
Calvez, P., Breukink, E., Roper, D. I., Dib, M., Contreras-Martel, C., & Zapun, A. (2017). Substitutions in PBP2b from β-lactam resistant Streptococcus pneumoniae have different effects on enzymatic activity and drug reactivity. Journal of Biological Chemistry, 292(7), 2854-2865. https://doi.org/10.1074/jbc.M116.764696

Overige resultaten

Medeiros-Silva, J., Jekhmane, S., Breukink, E., & Weingarth, M. (2017). Magic bullets to fight resistance: Uncovering how peptide-antibiotics break down the bacterial cell envelope. The FEBS journal, 284(S1), 52. https://doi.org/10.1111/febs.14170

2016

Wetenschappelijke publicaties

Liu, Y., & Breukink, E. (2016). The membrane steps of bacterial cell wall synthesis as antibiotic targets. Antibiotics, 5(3), 28. https://doi.org/10.3390/antibiotics5030028
Kleijn, L. H. J., Oppedijk, S. F., T Hart, P., van Harten, R., Martin-Visscher, L. A., Kemmink, J., Breukink, E., & Martin, N. I. (2016). Total Synthesis of Laspartomycin C and Characterization of Its Antibacterial Mechanism of Action. Journal of Medicinal Chemistry, 59(7), 3569-3574. https://doi.org/10.1021/acs.jmedchem.6b00219
Cleverley, R. M., Rismondo, J., Lockhart-Cairns, M. P., Van Bentum, P. T., Egan, A. J. F., Vollmer, W., Halbedel, S., Baldock, C., Breukink, E., & Lewis, R. J. (2016). Subunit arrangement in GpsB, a regulator of cell wall biosynthesis. Microbial Drug Resistance, 22(6), 446-460. https://doi.org/10.1089/mdr.2016.0050
Van 't Veer, I. L., Leloup, N., Egan, A., Janssen, B., Martin, N., Vollmer, W., & Breukink, E. (2016). Site specific immobilization of the peptidoglycan synthase PBP1B on a surface plasmon resonance chip surface. ChemBioChem, 17(23), 2250-2256. https://doi.org/10.1002/cbic.201600461
Broekgaarden, M., Van Vught, R., Oliveira, S., Roovers, R. C., Van Bergen En Henegouwen, P. M. P., Pieters, R. J., Van Gulik, T. M., Breukink, E., & Heger, M. (2016). Site-specific conjugation of single domain antibodies to liposomes enhances photosensitizer uptake and photodynamic therapy efficacy. Nanoscale, 8(12), 6490-6494. https://doi.org/10.1039/c6nr00014b
https://dspace.library.uu.nl/bitstream/handle/1874/339445/c6nr00014b.pdf?sequence=1
Liu, Y., Garcia Lopes Maia Rodrigues, J., Bonvin, A. M. J. J., Zaal, E. A., Berkers, C. R., Heger, M., Gawarecka, K., Swiezewska, E., Breukink, E., & Egmond, M. R. (2016). New insight into the catalytic mechanism of bacterial mray from enzyme kinetics and docking studies. Journal of Biological Chemistry, 291(29), 15057-15068. https://doi.org/10.1074/jbc.M116.717884
't Hart, P., Oppedijk, S. F., Breukink, E., & Martin, N. I. (2016). New Insights into Nisin's Antibacterial Mechanism Revealed by Binding Studies with Synthetic Lipid II Analogues. Biochemistry, 55(1), 232–237. https://doi.org/10.1021/acs.biochem.5b01173
Oppedijk, S. F., Martin, N. I., & Breukink, E. (2016). Hit 'em where it hurts: The growing and structurally diverse family of peptides that target lipid-II. Biochimica et Biophysica Acta-Biomembranes, 1858(5), 947–957. https://doi.org/10.1016/j.bbamem.2015.10.024

2015

Wetenschappelijke publicaties

Scheidelaar, S., Swainsbury, D., Koorengevel, M., Meeldijk, H., Breukink, E., van Grondelle, R., Jones, M., & Killian, J. A. (2015). Detergent-Free Extraction of the Reaction Center from Rhodobacter sphaeroides into Native Nanodiscs. Nanodisc Size Matters! Biophysical Journal, 108(2). https://doi.org/10.1016/j.bpj.2014.11.3048
Sosič, I., Anderluh, M., Sova, M., Gobec, M., Mlinarič Raščan, I., Derouaux, A., Amoroso, A., Terrak, M., Breukink, E., & Gobec, S. (2015). Structure-Activity Relationships of Novel Tryptamine-Based Inhibitors of Bacterial Transglycosylase. Journal of Medicinal Chemistry. https://doi.org/10.1021/acs.jmedchem.5b01482
Blanchaert, B., Wyseure, T., Breukink, E., Adams, E., Declerck, P., & Van Schepdael, A. (2015). Development of a liquid chromatography/mass spectrometry assay for the bacterial transglycosylation reaction through measurement of Lipid II. Electrophoresis, 36(21-22), 2841-2849. https://doi.org/10.1002/elps.201500201
Egan, A. J. F., Biboy, J., van 't Veer, I., Breukink, E., & Vollmer, W. (2015). Activities and regulation of peptidoglycan synthases. Philosophical transactions / Royal Society of London. Biological sciences, 370(1679), [20150031]. https://doi.org/10.1098/rstb.2015.0031
Bury, D., Dahmane, I., Derouaux, A., Dumbre, S., Herdewijn, P., Matagne, A., Breukink, E., Mueller-Seitz, E., Petz, M., & Terrak, M. (2015). Positive cooperativity between acceptor and donor sites of the peptidoglycan glycosyltransferase. Biochemical Pharmacology, 93(2), 141-150. https://doi.org/10.1016/j.bcp.2014.11.003
Scheidelaar, S., Koorengevel, M. C., Pardo, J. D., Meeldijk, J. D., Breukink, E., & Killian, J. A. (2015). Molecular Model for the solubilization of membranes into nanodisks by styrene maleic acid copolymers. Biophysical Journal, 108(2), 279-290. https://doi.org/10.1016/j.bpj.2014.11.3464
Weijer, R., Broekgaarden, M., Kos, M., van Vught, R., Rauws, E. A. J., Breukink, E., van Gulik, T. M., Storm, G., & Heger, M. (2015). Enhancing photodynamic therapy of refractory solid cancers: Combining second-generation photosensitizers with multi-targeted liposomal delivery. Journal of Photochemistry and Photobiology C: Photochemistry Reviews, 23, 103-131. https://doi.org/10.1016/j.jphotochemrev.2015.05.002
Slootweg, J. C., Van Herwerden, E. F., Van Doremalen, M. F. M., Breukink, E., Liskamp, R. M. J., & Rijkers, D. T. S. (2015). Synthesis of nisin AB dicarba analogs using ring-closing metathesis: Influence of sp<sup>3</sup>versus sp<sup>2</sup> hybridization of the α-carbon atom of residues dehydrobutyrine-2 and dehydroalanine-5 on the lipid II binding affinity. Organic & Biomolecular Chemistry, 13(21), 5997-6009. https://doi.org/10.1039/c5ob00336a
Koopmans, T., Wood, T. M., 'T Hart, P., Kleijn, L. H. J., Hendrickx, A. P. A., Willems, R. J. L., Breukink, E., & Martin, N. I. (2015). Semisynthetic Lipopeptides Derived from Nisin Display Antibacterial Activity and Lipid II Binding on Par with That of the Parent Compound. Journal of the American Chemical Society, 137(29), 9382-9389. https://doi.org/10.1021/jacs.5b04501
Gray, A. N., Egan, A. J. F., van 't Veer, I., Verheul, J., Colavin, A., Koumoutsi, A., Biboy, J., Altelaar, A. F. M., Damen, M. J., Huang, K. C., Simorre, J-P., Breukink, E., den Blaauwen, T., Typas, A., Gross, C. A., & Vollmer, W. (2015). Coordination of peptidoglycan synthesis and outer membrane constriction during Escherichia coli cell division. eLife, 4. https://doi.org/10.7554/eLife.07118

2014

Wetenschappelijke publicaties

Scheidelaar, S., Koorengevel, M., Swainsbury, D., Meeldijk, H., Breukink, E., Jones, M., van Grondelle, R., & Killian, A. (2014). Detergent-Free Extraction of Membrane Proteins into Native Nanodiscs. Application to the Reaction center of Rhodobacter Sphaeroides. Biophysical Journal, 106(2), 90a. https://doi.org/10.1016/j.bpj.2013.11.572
Harmsen, R. A. G., Ghalit, N., Kemmink, J., Breukink, E., Liskamp, R. M. J., & Rijkers, D. T. S. (2014). A conformationally constrained fused tricyclic nisin AB-ring system mimic toward an improved pyrophosphate binder of lipid II. Tetrahedron, 70(42), 7691-7699. https://doi.org/10.1016/j.tet.2014.06.023
Van Vught, R., Pieters, R. J., & Breukink, E. (2014). Site-specific functionalization of proteins and their applications to therapeutic antibodies. Computational and Structural Biotechnology Journal, 9(14), [e201402001]. https://doi.org/10.5936/csbj.201402001
Egan, A. J. F., Jean, N. L., Koumoutsi, A., Bougault, C. M., Biboy, J., Sassine, J., Solovyova, A. S., Breukink, E., Typas, A., Vollmer, W., & Simorre, J. P. (2014). Outer-membrane lipoprotein LpoB spans the periplasm to stimulate the peptidoglycan synthase PBP1B. Proceedings of the National Academy of Sciences of the United States of America, 111(22), 8197-8202. https://doi.org/10.1073/pnas.1400376111
Mohammadi, T., & Breukink, E. (2014). The chlamydial anomaly clarified? ChemBioChem, 15(10), 1391-1392. https://doi.org/10.1002/cbic.201402143
Slootweg, J. C., Peters, N., Quarles Van Ufford, H. C., Breukink, E., Liskamp, R. M. J., & Rijkers, D. T. S. (2014). Semi-synthesis of biologically active nisin hybrids composed of the native lanthionine ABC-fragment and a cross-stapled synthetic DE-fragment. Bioorganic & Medicinal Chemistry, 22(19), 5345-5353. https://doi.org/10.1016/j.bmc.2014.07.046
Mohammadi, T., Sijbrandi, R., Lutters, M., Verheul, J., Martin, N. I., den Blaauwen, T., de Kruijff, B., & Breukink, E. (2014). Specificity of the transport of lipid II by FtsW in Escherichia coli. Journal of Biological Chemistry, 289(21), 14707-14718. https://doi.org/10.1074/jbc.M114.557371

Populariserende publicaties

Slootweg, J. C., Van Schaik, T. B., Van Ufford, H. C. Q., Breukink, E., Liskamp, R. M. J., & Rijkers, D. S. (2014). Synthese en toepassing van een lipofiel aminozuurderivaat voor het verbeteren van de membraanaffiniteit van antimicrobiële peptiden. Pharmaceutisch Weekblad, 149(43), 174-179. https://www.scopus.com/record/display.uri?eid=2-s2.0-84949966040&origin=inward&txGid=54b908454fc67400cac49c3a0f33d69c

2013

Wetenschappelijke publicaties

Paiva, A. D., & Breukink, E. (2013). Antimicrobial peptides produced by microorganisms. In Antimicrobial Peptides and Innate Immunity (pp. 53-95). Springer Basel AG. https://doi.org/10.1007/978-3-0348-0541-4_3
Scheidelaar, S., Koorengevel, M. C., Meeldijk, H., Breukink, E., & Killian, A. (2013). Towards Detergent Free Solubilization of Membrane Proteins into Nanodiscs: A Biophysical Study on the Interaction between Styrene Maleic Acid (SMA) Copolymers and Synthetic Phospholipid Vesicles. Biophysical Journal, 104(2). https://doi.org/10.1016/j.bpj.2012.11.3313
Imran, M., Revol-Junelles, A-M., Diepeveen-de Bruin, M., Paris, C., Breukink, E. J., & Desobry, S. (2013). Fluorescent labeling of nisin Z and assessment of anti-listerial action. Journal of Microbiological Methods, 95(2), 107-113. https://doi.org/10.1016/j.mimet.2013.07.009
Slootweg, J. C., van Schaik, T. B., Quarles van Ufford, H. C., Breukink, E. J., Liskamp, R. M. J., & Rijkers, D. T. S. (2013). Improving the biological activity of the antimicrobial peptide anoplin by membrane anchoring through a lipophilic amino acid derivative. Bioorganic & Medicinal Chemistry Letters, 23, 3749-3752. https://doi.org/10.1016/j.bmcl.2013.05.002

Overige resultaten

Hoekstra, J., Jenneskens, L. W., Geus, J. W., & Breukink, E. J. (2013). Amorphous Carbon Supported Nanoparticles Comprising Oxides Of Lanthanides And Method For Preparing Them. (Patent No. WO2013/144879).

2012

Wetenschappelijke publicaties

Arnusch, C. J., Pieters, R. J., & Breukink, E. (2012). Enhanced membrane pore formation through high-affinity targeted antimicrobial peptides. PLoS One, 7(6), [e39768]. https://doi.org/10.1371/journal.pone.0039768
Banzhaf, M., van den Berg van Saparoea, B., Terrak, M., Fraipont, C., Egan, A., Philippe, J., Zapun, A., Breukink, E., Nguyen-Distèche, M., den Blaauwen, T., & Vollmer, W. (2012). Cooperativity of peptidoglycan synthases active in bacterial cell elongation. Molecular Microbiology, 85(1), 179-194. https://doi.org/10.1111/j.1365-2958.2012.08103.x
Nikolaidis, I., Izoré, T., Job, V., Thielens, N., Breukink, E., & Dessen, A. (2012). Calcium-dependent complex formation between PBP2 and lytic transglycosylase SltB1 of Pseudomonas aeruginosa. Microbial Drug Resistance, 18(3), 298-305. https://doi.org/10.1089/mdr.2012.0006
Helassa, N., Vollmer, W., Breukink, E., Vernet, T., & Zapun, A. (2012). The membrane anchor of penicillin-binding protein PBP2a from Streptococcus pneumoniae influences peptidoglycan chain length. The FEBS journal, 279(11), 2071-81. https://doi.org/10.1111/j.1742-4658.2012.08592.x
Paiva, A. D., Irving, N., Breukink, E., & Mantovani, H. C. (2012). Interaction with lipid II induces conformational changes in bovicin HC5 structure. Antimicrobial Agents and Chemotherapy, 56(9), 4586-4593. https://doi.org/10.1128/AAC.00295-12
Paiva, A. D., de Oliveira, M. D., de Paula, S. O., Baracat-Pereira, M. C., Breukink, E., & Mantovani, H. C. (2012). Toxicity of bovicin HC5 against mammalian cell lines and the role of cholesterol in bacteriocin activity. Microbiology, 158(Pt 11), 2851-8. https://doi.org/10.1099/mic.0.062190-0
Broekgaarden, M., Van Vught, R., De Kroon, A. I. P. M., Breukink, E., Van Gulik, T. M., & Heger, M. (2012). Assessment of in vitro efficiency of multi-targeting liposomes containing zinc-phthalocyanine for photodynamic therapy. Lasers in Surgery and Medicine, 44, 38-39. https://doi.org/10.1002/lsm.22023
Te Welscher, Y. M., Van Leeuwen, M. R., De Kruijff, B., Dijksterhuis, J., & Breukink, E. (2012). Polyene antibiotic that inhibits membrane transport proteins. Proceedings of the National Academy of Sciences of the United States of America, 109(28), 11156-11159. https://doi.org/10.1073/pnas.1203375109
Mulder, M. P. C., Fodran, P., Kemmink, J., Breukink, E. J., Kruijtzer, J. A. W., Minnaard, A. J., & Liskamp, R. M. J. (2012). Mutual influence of backbone proline substitution and lipophilic tail character on the biological activity of simplified analogues of caspofungin. Organic and Biomolecular Chemistry, 10(37), 7491-7502. https://doi.org/10.1039/c2ob25951f
Mulder, M. P. C., Fodran, P., Kemmink, J., Breukink, E. J., Kruijtzer, J. A. W., Minnaard, A. J., & Liskamp, R. M. J. (2012). Mutual influence of backbone proline substitution and lipopholic tail character on the biological activity of simplified analogues of caspofungin. Organic & Biomolecular Chemistry, 10, 7491-7502.

2011

Wetenschappelijke publicaties

Derouaux, A., Turk, S., Olrichs, N. K., Gobec, S., Breukink, E., Amoroso, A., Offant, J., Bostock, J., Mariner, K., Chopra, I., Vernet, T., Zervosen, A., Joris, B., Frère, J-M., Nguyen-Distèche, M., & Terrak, M. (2011). Small molecule inhibitors of peptidoglycan synthesis targeting the lipid II precursor. Biochemical Pharmacology, 81(9), 1098-105. https://doi.org/10.1016/j.bcp.2011.02.008
Paiva, A. D., Breukink, E., & Mantovani, H. C. (2011). Role of lipid II and membrane thickness in the mechanism of action of the lantibiotic bovicin HC5. Antimicrobial Agents and Chemotherapy, 55(11), 5284-5293. https://doi.org/10.1128/AAC.00638-11
Olrichs, N. K., Aarsman, M. E. G., Verheul, J., Arnusch, C. J., Martin, N. I., Hervé, M., Vollmer, W., de Kruijff, B., Breukink, E., & den Blaauwen, T. (2011). A Novel in vivo Cell-Wall Labeling Approach Sheds New Light on Peptidoglycan Synthesis in Escherichia coli. ChemBioChem, 12(7), 1124-1133. https://doi.org/10.1002/cbic.201000552
Mohammadi, T., Van Dam, V., Sijbrandi, R., Vernet, T., Zapun, A., Bouhss, A., Diepeveen-De Bruin, M., Nguyen-Distéche, M., De Kruijff, B., & Breukink, E. (2011). Identification of FtsW as a transporter of lipid-linked cell wall precursors across the membrane. EMBO Journal, 30(8), 1425-1432. https://doi.org/10.1038/emboj.2011.61
Olrichs, N. K., Aarsman, M. E. G., Verheul, J., Arnusch, C. J., Martin, N. I., Herve, M., Vollmer, W., de Kruijff, B., Breukink, E. J., & den Blaauwen, T. A. (2011). A novel in vivo cell-wall labelling approach sheds new light on peptidoglycan synthesis in Escherichia coli. ChemBioChem, 12, 1124-1133.

2010

Wetenschappelijke publicaties

Typas, A., Banzhaf, M., van den Berg van Saparoea, B., Verheul, J., Biboy, J., Nichols, R. J., Zietek, M., Beilharz, K., Kannenberg, K., von Rechenberg, M., Breukink, E., den Blaauwen, T., Gross, C. A., & Vollmer, W. (2010). Regulation of peptidoglycan synthesis by outer-membrane proteins. Cell, 143(7), 1097-109. https://doi.org/10.1016/j.cell.2010.11.038
Offant, J., Terrak, M., Derouaux, A., Breukink, E. J., Nguyen-Distèche, M., Zapun, A., & Vernet, T. (2010). Optimization of conditions for the glycosyltransferase activity of penicillin-binding protein 1a from Thermotoga maritima. The FEBS journal, 277(20), 4290-4298. https://doi.org/10.1111/j.1742-4658.2010.07817.x

2009

Wetenschappelijke publicaties

Pieters, R. J., Arnusch, C. J., & Breukink, E. J. (2009). Membrane permeabilization by multivalent anti-microbial peptides. Proteins peptide letters, 16, 736-742.

2008

Wetenschappelijke publicaties

Kramer, N. E., Hasper, H. E., van den Bogaard, P. T. C., Morath, S., de Kruijff, B., Hartung, T., Smid, E. J., Breukink, E. J., Kok, J., & Kuipers, O. P. (2008). Increased D-alanylation of lipoteichoic acid and a thickened septum are main determinants in the nisin resistance mechanism of Lactococcus lactis. Microbiology, 154, 1755-1762.
https://dspace.library.uu.nl/bitstream/handle/1874/33262/10Kramer-%2520Increased.pdf?sequence=1
Terrak, M., Sauvage, E., Derouaux, A., Dehareng, D., Bouhss, A., Breukink, E. J., Jeanjean, S., & Nguyen-Distèche, M. (2008). Importance of the conserved residues in the peptidoglycan glycosyltransferase module of the class A penicillin-binding protein 1b of Escherichia coli. Journal of Biological Chemistry, 283(42), 28464-28470.
https://dspace.library.uu.nl/bitstream/handle/1874/33206/14Terrak-Importance.pdf?sequence=1
Derouaux, A., Wolf, B., Fraipont, C., Breukink, E. J., Nguyen-Distèche, M., & Terrak, M. (2008). The monofunctional glycosyltransferase of Escherichia coli localizes to the cell division site and interacts with the penicillin-binding protein 3 (PBP3), FtsW and FtsN. Journal of Bacteriology, 190(5), 1831-1834.
te Welscher, Y. M., ten Napel, H. H., Masià Balagué, M., Souza, C. M., Riezman, H., de Kruijff, B., & Breukink, E. J. (2008). Natamycin blocks fungal growth by binding specifically to ergosterol without permeabilizing the membrane. Journal of Biological Chemistry, 283(10), 6393-6401.
Arnusch, C. J., Bonvin, A. M. J. J., Verel, A. M., Jansen, W. T. M., Liskamp, R. M. J., de Kruijff, B., Pieters, R. J., & Breukink, E. J. (2008). The vancomycin-nisin(1-12) hybrid restores activity against vancomycin resistant enterococci. Biochemistry, 47(48), 12661-12663. https://doi.org/10.1021/bi801597b
Parisot, J., Carey, S., Breukink, E. J., Chan, W. C., Narbad, A., & Bonev, B. B. (2008). Molecular mechanism of target recognition by Subtilin, a class I Lanthionine antibiotic. Antimicrobial Agents and Chemotherapy, 52(2), 612-618.

2007

Wetenschappelijke publicaties

Arnusch, C. J., Branderhorst, H., De Kruijff, B., Liskamp, R. M. J., Breukink, E., & Pieters, R. J. (2007). Enhanced membrane pore formation by multimeric/oligomeric antimicrobial peptides. Biochemistry, 46(46), 13437-13442. https://doi.org/10.1021/bi7015553
Hasegawa, M., Kawasaki, A., Yang, K., Fujimoto, Y., Masumoto, J., Breukink, E. J., Nuñez, G., Fukase, K., & Inohara, N. (2007). A role of lipophilic peptidoglycan-related molecules in induction of Nod1-mediated immune responses. Journal of Biological Chemistry, 282(16), 11757-11764.
Ghalit, N., Reichwein, J. F., Hilbers, H. W., Breukink, E. J., Rijkers, D. T. S., & Liskamp, R. M. J. (2007). Synthesis of bicyclic alkene/alkane-bridged nisin mimics by ring-closing metathesis and their biochemical evaluation as Lipid II binders: toward the design of potential novel antibiotics. ChemBioChem, 8, 1540-1554.
https://dspace.library.uu.nl/bitstream/handle/1874/26104/Ghalit-Synthesis%2520of%2520bicyclic.pdf?sequence=1

2006

Wetenschappelijke publicaties

Ganchev, D. N., Hasper, H. E., Breukink, E., & De Kruijff, B. (2006). Size and orientation of the lipid II headgroup as revealed by AFM imaging. Biochemistry, 45(19), 6195-6202. https://doi.org/10.1021/bi051913e
Hsu, S. T., Breukink, E. J., & Kaptein, R. (2006). Structural motifs of lipid II-binding lantibiotics as a blueprint for novel antibiotics. Anti-Infective Agents in Medicinal Chemistry, 5(3), 245-254.
Ganchev, D. N., Hasper, H. E., Breukink, E. J., & de Kruijff, B. (2006). Size and orientation of the Lipid II head group as revealed by AFM imaging. Biochemistry, 45, 6195-6202.
https://dspace.library.uu.nl/bitstream/handle/1874/19982/Ganchev-Size%2520and%2520orientation.pdf?sequence=1

Populariserende publicaties

Breukink, E. (2006). Nieuw wapen tegen resistentie. Pharmaceutisch Weekblad, 141(26), 886-887.

2004

Wetenschappelijke publicaties

Hasper, H. E., De Kruijff, B., & Breukink, E. (2004). Assembly and stability of nisin-Lipid II pores. Biochemistry, 43(36), 11567-11575. https://doi.org/10.1021/bi049476b
Hsu, S-T. D., Breukink, E., Tischenko, E., Lutters, M. A. G., De Kruijff, B., Kaptein, R., Bonvin, A. M. J. J., & Van Nuland, N. A. J. (2004). The nisin-lipid II complex reveals a pyrophosphate cage that provides a blueprint for novel antibiotics. Nature Structural and Molecular Biology, 11(10), 963-967. https://doi.org/10.1038/nsmb830
Bonev, B. B., Breukink, E. J., Swiezewska, E., de Kruijff, B., & Watts, A. (2004). Targeting extracellular pyrophosphates underpins the high selectivity of nisin. FASEB Journal, 18, 1862-1869.
https://dspace.library.uu.nl/bitstream/handle/1874/14608/Bonev-Targeting%2520extracellular.pdf?sequence=1

2003

Wetenschappelijke publicaties

Hsu, S-T. D., Breukink, E., Bierbaum, G., Sahl, H-G., De Kruijff, B., Kaptein, R., Van Nuland, N. A. J., & Bonvin, A. M. J. J. (2003). NMR study of mersacidin and lipid II interaction in dodecylphosphocholine micelles: Conformational changes are a key to antimicrobial activity. Journal of Biological Chemistry, 278(15), 13110-13117. https://doi.org/10.1074/jbc.M211144200

2002

Wetenschappelijke publicaties

Wills, M., Morris, D., Breukink, E., Bonnici, P. J., & Heck, A. J. R. (2002). Stereocontrolled synthesis of angularly fused tricyclic ring systems by means of 1-metalla-1,3,5-hexatrienes (M = Cr, W). Chemistry - A European Journal, 8(4), 910-916. https://doi.org/10.1002/1521-3765(20020215)8:4<910::AID-CHEM910>3.0.CO;2-T
Hsu, S., Breukink, E. J., de Kruijff, B., Kaptein, R., Bonvin, A. M. J. J., & van Nuland, N. A. J. (2002). Mapping the targeted membrane pore formation mechanism by solution NMR: the nisin Z and lipid II interaction in SDS micelles. Biochemistry, 41, 7670-7676. https://doi.org/10.1021/bi025679t

2001

Wetenschappelijke publicaties

Van Kraaij, C., Breukink, E., Rollema, H. S., Bongers, R. S., Kosters, H. A., De Kruijff, B., & Kuipers, O. P. (2001). Engineering a disulfide bond and free thiols in the lantibiotic nisin Z. European Journal of Biochemistry, 267(3), 901-909. https://doi.org/10.1046/j.1432-1327.2000.01075.x

2000

Wetenschappelijke publicaties

Breukink, E., Ganz, P., De Kruijff, B., & Seelig, J. (2000). Binding of nisin Z to bilayer vesicles as determined with isothermal titration calorimetry. Biochemistry, 39(33), 10247-10254. https://doi.org/10.1021/bi000915q

1999

Wetenschappelijke publicaties

Breukink, E., & De Kruijff, B. (1999). The lantibiotic nisin, a special case or not? Biochimica et Biophysica Acta - Biomembranes, 1462(1-2), 223-234. https://doi.org/10.1016/S0005-2736(99)00208-4
Lins, L., Ducarme, P., Breukink, E., & Brasseur, R. (1999). Computational study of nisin interaction with model membrane. Biochimica et Biophysica Acta - Biomembranes, 1420(1-2), 111-120. https://doi.org/10.1016/S0005-2736(99)00090-5
Breukink, E., Wiedemann, I., Van Kraaij, C., Kuipers, O. P., Sahl, H-G., & De Kruijff, B. (1999). Use of the cell wail precursor lipid II by a pore-forming peptide antibiotic. Science, 286(5448), 2361-2364. https://doi.org/10.1126/science.286.5448.2361

1998

Wetenschappelijke publicaties

Van Kraaij, C., Breukink, E., Noordermeer, M. A., Demel, R. A., Siezen, R. J., Kuipers, O. P., & De Kruijff, B. (1998). Pore formation by nisin involves translocation of its C-terminal part across the membrane. Biochemistry, 37(46), 16033-16040. https://doi.org/10.1021/bi980931b
Breukink, E., Van Kraaij, C., Van Dalen, A., Demel, R. A., Siezen, R. J., De Kruijff, B., & Kuipers, O. P. (1998). The orientation of nisin in membranes. Biochemistry, 37(22), 8153-8162. https://doi.org/10.1021/bi972797l

1997

Wetenschappelijke publicaties

Van Kraaij, C., Breukink, E., Rollema, H. S., Siezen, R. J., Demel, R. A., De Kruijff, B., & Kuipers, O. P. (1997). Influence of charge differences in the C-terminal part of nisin on antimicrobial activity and signaling capacity. European Journal of Biochemistry, 247(1), 114-120.
Breukink, E., Van Kraaij, C., Demel, R. A., Siezen, R. J., Kuipers, O. P., & De Kruijff, B. (1997). The C-terminal region of nisin is responsible for the initial interaction of nisin with the target membrane. Biochemistry, 36(23), 6968-6976. https://doi.org/10.1021/bi970008u
Den Blaauwen, T., De Wit, J. G., Gosker, H., Van Der Does, C., Breukink, E-J., De Leij, L., & Driessen, A. J. M. (1997). Inhibition of preprotein translocation and reversion of the membrane inserted state of SecA by a carboxyl terminus binding MAb. Biochemistry, 36(30), 9159-9168. https://doi.org/10.1021/bi970344a

1996

Wetenschappelijke publicaties

Van Raalte, A. L. J., Demel, R. A., Verberkmoes, G., Breukink, E., Keller, R. C. A., & De Kruijff, B. (1996). Influence of the signal sequence and chaperone SecB on the interaction between precursor protein prePhoE and phospholipids. European Journal of Biochemistry, 235(1-2), 207-214.

1995

Wetenschappelijke publicaties

Breukink, E., Nouwen, N., Van Raalte, A., Mizushima, S., Tommassen, J., & De Kruijff, B. (1995). The C terminus of SecA is involved in both lipid binding and SecB binding. Journal of Biological Chemistry, 270(14), 7902-7907. https://doi.org/10.1074/jbc.270.14.7902

1994

Wetenschappelijke publicaties

Kusters, R., Breukink, E., Gallusser, A., Kuhn, A., & De Kruijff, B. (1994). A dual role for phosphatidylglycerol in protein translocation across the Escherichia coli inner membrane. Journal of Biological Chemistry, 269(2), 1560-1563.

1993

Wetenschappelijke publicaties

Van der Wolk, J., Klose, M., Breukink, E., Demel, R. A., De Kruijff, B., Freudl, R., & Driessen, A. J. M. (1993). Characterization of a Bacillus subtilis SecA mutant protein deficient in translocation ATPase and release from the membrane. Molecular Microbiology, 8(1), 31-42.
Breukink, E., Keller, R. C. A., & De Kruijff, B. (1993). Nucleotide and negatively charged lipid-dependent vesicle aggregation caused by SecA. Evidence that SecA contains two lipid-binding sites. FEBS Letters, 331(1-2), 19-24. https://doi.org/10.1016/0014-5793(93)80289-7

1992

Wetenschappelijke publicaties

Kruijff, B. D., Breukink, E., Demel, R. A., T Hof, R. V., Jongh, H. H. J. D., Jordi, W., Keller, R. C. A., Killian, J. A., Kroon, A. I. P. M. D., Kusters, R., & Pilon, M. (1992). Lipid involvement in protein translocation. New Comprehensive Biochemistry, 22(C), 85-101. https://doi.org/10.1016/S0167-7306(08)60085-X
Breukink, E., Demel, R. A., De Korte-Kool, G., & De Kruijff, B. (1992). SecA insertion into phospholipids is stimulated by negatively charged lipids and inhibited by ATP: A monolayer study. Biochemistry, 31(4), 1119-1124.
Breukink, E., Kusters, R., & De Kruijff, B. (1992). In-vitro studies on the folding characteristics of the Escherichia coli precursor protein prePhoE. Evidence that SecB prevents the precursor from aggregating by forming A functional complex. European Journal of Biochemistry, 208(2), 419-425.

1989

Wetenschappelijke publicaties

Kusters, R., de Vrije, T., Breukink, E., & De Kruijff, B. (1989). SecB protein stabilizes a translocation-competent state of purified prePhoE protein. Journal of Biological Chemistry, 264(35), 20827-20830.