Publicaties
2023
Wetenschappelijke publicaties
Shukla, R., Peoples, A. J., Ludwig, K. C., Maity, S.
, Derks, M. G. N., de Benedetti, S., Krueger, A. M., Vermeulen, B. J. A.
, Lavore, F., Honorato, R. V., Grein, F.
, Bonvin, A., Kubitscheck, U.
, Breukink, E., Achorn, C., Nitti, A., Schwalen, C. J., Spoering, A. L., Ling, L. L.
, ... Weingarth, M. (2023).
A new antibiotic from an uncultured bacterium binds to an immutable target.
bioRxiv.
https://doi.org/10.1101/2023.05.15.540765 Wang, X., van Beekveld, R. A. M., Xu, Y., Parmar, A., Das, S., Singh, I.
, & Breukink, E. (2023).
Analyzing mechanisms of action of antimicrobial peptides on bacterial membranes requires multiple complimentary assays and different bacterial strains.
Biochimica et Biophysica Acta - Biomembranes,
1865(6), [184160].
https://doi.org/10.1016/j.bbamem.2023.184160https://dspace.library.uu.nl/bitstream/handle/1874/429607/1_s2.0_S0005273623000421_main.pdf?sequence=1 2022
Wetenschappelijke publicaties
van Beekveld, R. A. M., Derks, M. G. N., Kumar, R., Smid, L., Maass, T.
, Medeiros-Silva, J., Breukink, E., & Weingarth, M. (2022).
Specific Lipid Studies in Complex Membranes by Solid-State NMR Spectroscopy.
Chemistry - A European Journal,
28(70), [e202202472].
https://doi.org/10.1002/chem.202202472 Xu, Y., Hernández-Rocamora, V. M.
, Lorent, J. H., Cox, R., Wang, X., Bao, X., Stel, M., Vos, G., van den Bos, R. M., Pieters, R. J., Gray, J., Vollmer, W.
, & Breukink, E. (2022).
Metabolic labeling of the bacterial peptidoglycan by functionalized glucosamine.
iScience,
25(8), [104753].
https://doi.org/10.1016/j.isci.2022.104753 Shukla, R., Lavore, F., Maity, S.
, Derks, M. G. N., Jones, C. R., Vermeulen, B. J. A., Melcrová, A., Morris, M. A., Becker, L. M.
, Wang, X., Kumar, R., Medeiros-Silva, J., van Beekveld, R. A. M., Bonvin, A. M. J. J., Lorent, J. H., Lelli, M., Nowick, J. S.
, MacGillavry, H. D., Peoples, A. J.
, ... Weingarth, M. (2022).
Teixobactin kills bacteria by a two-pronged attack on the cell envelope.
Nature,
608(7922), 390-396.
https://doi.org/10.1038/s41586-022-05019-y 2021
Wetenschappelijke publicaties
Li, Y., Boes, A., Cui, Y.
, Zhao, S., Liao, Q., Gong, H.
, Breukink, E., Lutkenhaus, J., Terrak, M., & Du, S. (2021).
Identification of the potential active site of the septal peptidoglycan polymerase FtsW.
PLoS Genetics,
18(1), 1-25. [e1009993].
https://doi.org/10.1371/journal.pgen.1009993Zhao, X.
, Wang, X., Shukla, R., Kumar, R., Weingarth, M., Breukink, E., & Kuipers, O. P. (2021).
Brevibacillin 2V Exerts Its Bactericidal Activity via Binding to Lipid II and Permeabilizing Cellular Membranes.
Frontiers in Microbiology,
12, 1-13. [694847].
https://doi.org/10.3389/fmicb.2021.694847Zhao, X.
, Wang, X., Shukla, R., Kumar, R., Weingarth, M., Breukink, E., & Kuipers, O. P. (2021).
Brevibacillin 2V, a Novel Antimicrobial Lipopeptide With an Exceptionally Low Hemolytic Activity.
Frontiers in Microbiology,
12, 1-11. [693725].
https://doi.org/10.3389/fmicb.2021.693725van Eijk, M., van Dijk, A., van der Ent, C. K., Arets, H. G. M.
, Breukink, E., van Os, N., Adrichem, R., van der Water, S., Gomez, R. L., Kristensen, M., Hessing, M., Jekhmane, S.
, Weingarth, M., Veldhuizen, R. A. W.
, Veldhuizen, E. J. A., & Haagsman, H. P. (2021).
PepBiotics, novel cathelicidin-inspired antimicrobials to fight pulmonary bacterial infections.
Biochimica et Biophysica Acta - General Subjects,
1865(9), [129951].
https://doi.org/10.1016/j.bbagen.2021.129951 Sassine, J., Pazos, M.
, Breukink, E., & Vollmer, W. (2021).
Lytic transglycosylase MltG cleaves in nascent peptidoglycan and produces short glycan strands.
The Cell Surface,
7, 1-9. [100053].
https://doi.org/10.1016/j.tcsw.2021.100053Hernández-Rocamora, V. M., Baranova, N., Peters, K.
, Breukink, E., Loose, M., & Vollmer, W. (2021).
Real-time monitoring of peptidoglycan synthesis by membrane-reconstituted penicillin-binding proteins.
eLife,
10, 1-79. [e61525].
https://doi.org/10.7554/eLife.615252020
Wetenschappelijke publicaties
Boes, A., Kerff, F., Herman, R., Touzé, T.
, Breukink, E., & Terrak, M. (2020).
The bacterial cell division protein fragment E FtsN binds to and activates the major peptidoglycan synthase PBP1b.
Journal of Biological Chemistry,
295(52), 18256-18265.
https://doi.org/10.1074/jbc.ra120.015951Pazos, M., Peters, K., Boes, A., Safaei, Y., Kenward, C., Caveney, N. A., Laguri, C.
, Breukink, E., Strynadka, N. C. J., Simorre, J. P., Terrak, M., & Vollmer, W. (2020).
SPOR Proteins Are Required for Functionality of Class A Penicillin-Binding Proteins in Escherichia coli.
mBio,
11(6).
https://doi.org/10.1128/mBio.02796-20Boes, A., Brunel, J. M., Derouaux, A., Kerff, F., Bouhss, A., Touze, T.
, Breukink, E., & Terrak, M. (2020).
Squalamine and aminosterol mimics inhibit the peptidoglycan glycosyltransferase activity of pbp1b.
Antibiotics,
9(7), [373].
https://doi.org/10.3390/antibiotics9070373Shukla, R., Medeiros-Silva, J., Parmar, A., Vermeulen, B. J. A., Das, S.
, Paioni, A. L., Jekhmane, S.
, Lorent, J., Bonvin, A. M. J. J., Baldus, M., Lelli, M.
, Veldhuizen, E. J. A., Breukink, E., Singh, I.
, & Weingarth, M. (2020).
Mode of action of teixobactins in cellular membranes.
Nature Communications,
11(1), 2848.
https://doi.org/10.1038/s41467-020-16600-2 Banzhaf, M., Yau, H. C., Verheul, J., Lodge, A., Kritikos, G., Mateus, A., Cordier, B., Hov, A. K., Stein, F., Wartel, M., Pazos, M., Solovyova, A. S.
, Breukink, E., van Teeffelen, S., Savitski, M. M., den Blaauwen, T., Typas, A., & Vollmer, W. (2020).
Outer membrane lipoprotein NlpI scaffolds peptidoglycan hydrolases within multi-enzyme complexes in Escherichia coli.
EMBO Journal,
39(5), [e102246].
https://doi.org/10.15252/embj.2019102246Zhao, X., Yin, Z.
, Breukink, E., Moll, G. N., & Kuipers, O. P. (2020).
An Engineered Double Lipid II Binding Motifs-Containing Lantibiotic Displays Potent and Selective Antimicrobial Activity against Enterococcus faecium.
Antimicrobial Agents and Chemotherapy,
64(6), [e02050-19].
https://doi.org/10.1128/AAC.02050-19Boes, A., Olatunji, S.
, Mohammadi, T., Breukink, E., & Terrak, M. (2020).
Fluorescence anisotropy assays for high throughput screening of compounds binding to lipid II, PBP1b, FtsW and MurJ.
Scientific Reports,
10(1), [6280].
https://doi.org/10.1038/s41598-020-63380-2Caveney, N. A., Egan, A. J. F., Ayala, I., Laguri, C., Robb, C. S.
, Breukink, E., Vollmer, W., Strynadka, N. C. J., & Simorre, J-P. (2020).
Structure of the Peptidoglycan Synthase Activator LpoP in Pseudomonas aeruginosa.
Structure with Folding & design,
28(6), 643-650.e5.
https://doi.org/10.1016/j.str.2020.03.0122019
Wetenschappelijke publicaties
Kuru, E., Radkov, A., Meng, X., Egan, A., Alvarez, L., Dowson, A., Booher, G.
, Breukink, E., Roper, D. I., Cava, F., Vollmer, W., Brun, Y., & VanNieuwenhze, M. S. (2019).
Mechanisms of incorporation for D-amino acid probes that target peptidoglycan biosynthesis.
ACS Chemical Biology,
14(12), 2745-2756.
https://doi.org/10.1021/acschembio.9b00664Mueller, E. A., Egan, A. J.
, Breukink, E., Vollmer, W., & Levin, P. A. (2019).
Plasticity of Escherichia coli cell wall metabolism promotes fitness and antibiotic resistance across environmental conditions.
eLife,
2019(8), [e40754].
https://doi.org/10.7554/eLife.40754Pazos, M., Palacios, P., Vicente, M.
, Breukink, E., Peters, K., Casanova, M., Vollmer, W., & VanNieuwenhze, M. (2019).
Publisher Correction: Z-ring membrane anchors associate with cell wall synthases to initiate bacterial cell division.
Nature Communications,
10(1).
https://doi.org/10.1038/s41467-019-08410-yMorè, N., Martorana, A. M., Biboy, J., Otten, C., Winkle, M., Serrano, C. K. G., Montón Silva, A., Atkinson, L., Yau, H.
, Breukink, E., den Blaauwen, T., Vollmer, W., & Polissi, A. (2019).
Peptidoglycan Remodeling Enables Escherichia coli To Survive Severe Outer Membrane Assembly Defect.
mBio,
10(1), [e02729-18].
https://doi.org/10.1128/mBio.02729-18Medeiros-Silva, J., Jekhmane, S., Breukink, E., & Weingarth, M. (2019).
Towards the native binding modes of Lipid II targeting antibiotics.
ChemBioChem,
20(14), 1731-1738.
https://doi.org/10.1002/cbic.201800796 Boes, A., Olatunji, S.
, Breukink, E., & Terrak, M. (2019).
Regulation of the Peptidoglycan Polymerase Activity of PBP1b by Antagonist Actions of the Core Divisome Proteins FtsBLQ and FtsN.
mBio,
10(1), [e01912-18].
https://doi.org/10.1128/mBio.01912-182018
Wetenschappelijke publicaties
Liu, Y., Moura, E. C. C. M.
, Dörr, J. M., Scheidelaar, S., Heger, M., Egmond, M. R., Killian, J. A., Mohammadi, T., & Breukink, E. (2018).
Correction: Bacillus subtilis MraY in detergent-free system of nanodiscs wrapped by styrene-maleic acid copolymers.
PLoS One,
13(12), e0210135.
https://doi.org/10.1371/journal.pone.0210135 Pazos, M., Peters, K., Casanova, M., Palacios, P., VanNieuwenhze, M.
, Breukink, E., Vicente, M., & Vollmer, W. (2018).
Z-ring membrane anchors associate with cell wall synthases to initiate bacterial cell division.
Nature Communications,
9(1), [5090].
https://doi.org/10.1038/s41467-018-07559-2Liu, Y., Moura, E. C. C. M.
, Dörr, J. M., Scheidelaar, S., Heger, M., Egmond, M. R., Killian, J. A., Mohammadi, T., & Breukink, E. (2018).
Bacillus subtilis MraY in detergent-free system of nanodiscs wrapped by styrene-maleic acid copolymers.
PLoS One,
13(11), [e0206692].
https://doi.org/10.1371/journal.pone.0206692 Medeiros-Silva, J., Jekhmane, S., Paioni, A. L., Gawarecka, K.
, Baldus, M., Swiezewska, E.
, Breukink, E., & Weingarth, M. (2018).
High-resolution NMR studies of antibiotics in cellular membranes.
Nature Communications,
9(1), [3963].
https://doi.org/10.1038/s41467-018-06314-x Egan, A. J. F., Maya-Martinez, R., Ayala, I., Bougault, C. M., Banzhaf, M.
, Breukink, E., Vollmer, W., & Simorre, J-P. (2018).
Induced conformational changes activate the peptidoglycan synthase PBP1B.
Molecular Microbiology,
110(3), 335-356.
https://doi.org/10.1111/mmi.14082Hernández-Rocamora, V. M., Otten, C. F., Radkov, A., Simorre, J-P.
, Breukink, E., VanNieuwenhze, M., & Vollmer, W. (2018).
Coupling of polymerase and carrier lipid phosphatase prevents product inhibition in peptidoglycan synthesis.
Cell surface (Amsterdam),
2, 1-13.
https://doi.org/10.1016/j.tcsw.2018.04.002Jiang, H., Tang, X., Zhou, Q., Zou, J., Li, P.
, Breukink, E., & Gu, Q. (2018).
Plantaricin NC8 from Lactobacillus plantarum causes cell membrane disruption to Micrococcus luteus without targeting lipid II.
Applied Microbiology and Biotechnology,
102(17), 7465-7473.
https://doi.org/10.1007/s00253-018-9182-3Silva, A. M., Otten, C., Biboy, J.
, Breukink, E., Van Nieuwenhze, M., Vollmer, W., & den Blaauwen, T. (2018).
The fluorescent D-Amino Acid NADA as a tool to study the conditional activity of transpeptidases in Escherichia coli.
Frontiers in Microbiology,
9(SEP).
https://doi.org/10.3389/fmicb.2018.02101Morlot, C., Straume, D., Peters, K., Hegnar, O. A., Simon, N., Villard, A-M., Contreras-Martel, C., Leisico, F.
, Breukink, E., Gravier-Pelletier, C., Le Corre, L., Vollmer, W., Pietrancosta, N., Håvarstein, L. S., & Zapun, A. (2018).
Structure of the essential peptidoglycan amidotransferase MurT/GatD complex from Streptococcus pneumoniae.
Nature Communications,
9(1), [3180].
https://doi.org/10.1038/s41467-018-05602-w2017
Wetenschappelijke publicaties
Parmar, A., Iyer, A., Prior, S. H., Lloyd, D. G., Leng Goh, E. T., Vincent, C. S., Palmai-Pallag, T., Bachrati, C. Z.
, Breukink, E., Madder, A., Lakshminarayanan, R., Taylor, E. J., & Singh, I. (2017).
Teixobactin analogues reveal enduracididine to be non-essential for highly potent antibacterial activity and lipid II binding.
Chemical Science,
8(12), 8183-8192.
https://doi.org/10.1039/c7sc03241bLeclercq, S., Derouaux, A., Olatunji, S., Fraipont, C., Egan, A. J. F., Vollmer, W.
, Breukink, E., & Terrak, M. (2017).
Interplay between Penicillin-binding proteins and SEDS proteins promotes bacterial cell wall synthesis.
Scientific Reports,
7, [43306].
https://doi.org/10.1038/srep43306Parmar, A., Prior, S. H., Iyer, A., Vincent, C. S., Van Lysebetten, D.
, Breukink, E., Madder, A., Taylor, E. J., & Singh, I. (2017).
Defining the molecular structure of teixobactin analogues and understanding their role in antibacterial activities.
Chemical Communications,
53(12), 2016-2019.
https://doi.org/10.1039/c6cc09490b't Hart, P., Wood, T. M., Hajmohammadebrahimtehrani, K., van Harten, R. M., Sleszynska, M. K., Breukink, E. J., & Martin, N. I. (2017).
De novo identification of lipid II binding lipopeptides with antibacterial activity against vancomycin-resistant bacteria.
Chemical Science,
8, 7991-7997.
https://doi.org/10.1039/C7SC03413J Calvez, P.
, Breukink, E., Roper, D. I., Dib, M., Contreras-Martel, C., & Zapun, A. (2017).
Substitutions in PBP2b from β-lactam resistant Streptococcus pneumoniae have different effects on enzymatic activity and drug reactivity.
Journal of Biological Chemistry,
292(7), 2854-2865.
https://doi.org/10.1074/jbc.M116.764696Overige resultaten
Medeiros-Silva, J., Jekhmane, S., Breukink, E., & Weingarth, M. (2017).
Magic bullets to fight resistance: Uncovering how peptide-antibiotics break down the bacterial cell envelope.
The FEBS journal,
284(S1), 52.
https://doi.org/10.1111/febs.14170 2016
Wetenschappelijke publicaties
Kleijn, L. H. J., Oppedijk, S. F., T Hart, P., van Harten, R., Martin-Visscher, L. A.
, Kemmink, J., Breukink, E., & Martin, N. I. (2016).
Total Synthesis of Laspartomycin C and Characterization of Its Antibacterial Mechanism of Action.
Journal of Medicinal Chemistry,
59(7), 3569-3574.
https://doi.org/10.1021/acs.jmedchem.6b00219 Cleverley, R. M., Rismondo, J., Lockhart-Cairns, M. P.
, Van Bentum, P. T., Egan, A. J. F., Vollmer, W., Halbedel, S., Baldock, C.
, Breukink, E., & Lewis, R. J. (2016).
Subunit arrangement in GpsB, a regulator of cell wall biosynthesis.
Microbial Drug Resistance,
22(6), 446-460.
https://doi.org/10.1089/mdr.2016.0050Van 't Veer, I. L., Leloup, N., Egan, A.
, Janssen, B., Martin, N., Vollmer, W.
, & Breukink, E. (2016).
Site specific immobilization of the peptidoglycan synthase PBP1B on a surface plasmon resonance chip surface.
ChemBioChem,
17(23), 2250-2256.
https://doi.org/10.1002/cbic.201600461 Broekgaarden, M., Van Vught, R., Oliveira, S., Roovers, R. C., Van Bergen En Henegouwen, P. M. P., Pieters, R. J., Van Gulik, T. M.
, Breukink, E., & Heger, M. (2016).
Site-specific conjugation of single domain antibodies to liposomes enhances photosensitizer uptake and photodynamic therapy efficacy.
Nanoscale,
8(12), 6490-6494.
https://doi.org/10.1039/c6nr00014bhttps://dspace.library.uu.nl/bitstream/handle/1874/339445/c6nr00014b.pdf?sequence=1 Liu, Y., Garcia Lopes Maia Rodrigues, J., Bonvin, A. M. J. J., Zaal, E. A., Berkers, C. R., Heger, M., Gawarecka, K., Swiezewska, E.
, Breukink, E., & Egmond, M. R. (2016).
New insight into the catalytic mechanism of bacterial mray from enzyme kinetics and docking studies.
Journal of Biological Chemistry,
291(29), 15057-15068.
https://doi.org/10.1074/jbc.M116.717884 't Hart, P., Oppedijk, S. F., Breukink, E., & Martin, N. I. (2016).
New Insights into Nisin's Antibacterial Mechanism Revealed by Binding Studies with Synthetic Lipid II Analogues.
Biochemistry,
55(1), 232–237.
https://doi.org/10.1021/acs.biochem.5b01173 Oppedijk, S. F., Martin, N. I., & Breukink, E. (2016).
Hit 'em where it hurts: The growing and structurally diverse family of peptides that target lipid-II.
Biochimica et Biophysica Acta-Biomembranes,
1858(5), 947–957.
https://doi.org/10.1016/j.bbamem.2015.10.024 2015
Wetenschappelijke publicaties
Scheidelaar, S., Swainsbury, D.
, Koorengevel, M., Meeldijk, H., Breukink, E., van Grondelle, R., Jones, M.
, & Killian, J. A. (2015).
Detergent-Free Extraction of the Reaction Center from Rhodobacter sphaeroides into Native Nanodiscs. Nanodisc Size Matters! Biophysical Journal,
108(2).
https://doi.org/10.1016/j.bpj.2014.11.3048 Sosič, I., Anderluh, M., Sova, M., Gobec, M., Mlinarič Raščan, I., Derouaux, A., Amoroso, A., Terrak, M.
, Breukink, E., & Gobec, S. (2015).
Structure-Activity Relationships of Novel Tryptamine-Based Inhibitors of Bacterial Transglycosylase.
Journal of Medicinal Chemistry.
https://doi.org/10.1021/acs.jmedchem.5b01482Blanchaert, B., Wyseure, T.
, Breukink, E., Adams, E., Declerck, P., & Van Schepdael, A. (2015).
Development of a liquid chromatography/mass spectrometry assay for the bacterial transglycosylation reaction through measurement of Lipid II.
Electrophoresis,
36(21-22), 2841-2849.
https://doi.org/10.1002/elps.201500201Egan, A. J. F., Biboy, J.
, van 't Veer, I., Breukink, E., & Vollmer, W. (2015).
Activities and regulation of peptidoglycan synthases.
Philosophical transactions / Royal Society of London. Biological sciences,
370(1679), [20150031].
https://doi.org/10.1098/rstb.2015.0031Bury, D., Dahmane, I., Derouaux, A., Dumbre, S., Herdewijn, P., Matagne, A.
, Breukink, E., Mueller-Seitz, E., Petz, M., & Terrak, M. (2015).
Positive cooperativity between acceptor and donor sites of the peptidoglycan glycosyltransferase.
Biochemical Pharmacology,
93(2), 141-150.
https://doi.org/10.1016/j.bcp.2014.11.003Scheidelaar, S., Koorengevel, M. C., Pardo, J. D., Meeldijk, J. D., Breukink, E., & Killian, J. A. (2015).
Molecular Model for the solubilization of membranes into nanodisks by styrene maleic acid copolymers.
Biophysical Journal,
108(2), 279-290.
https://doi.org/10.1016/j.bpj.2014.11.3464 Weijer, R., Broekgaarden, M., Kos, M.
, van Vught, R., Rauws, E. A. J.
, Breukink, E., van Gulik, T. M.
, Storm, G., & Heger, M. (2015).
Enhancing photodynamic therapy of refractory solid cancers: Combining second-generation photosensitizers with multi-targeted liposomal delivery.
Journal of Photochemistry and Photobiology C: Photochemistry Reviews,
23, 103-131.
https://doi.org/10.1016/j.jphotochemrev.2015.05.002 Slootweg, J. C., Van Herwerden, E. F., Van Doremalen, M. F. M.
, Breukink, E., Liskamp, R. M. J., & Rijkers, D. T. S. (2015).
Synthesis of nisin AB dicarba analogs using ring-closing metathesis: Influence of sp<sup>3</sup>versus sp<sup>2</sup> hybridization of the α-carbon atom of residues dehydrobutyrine-2 and dehydroalanine-5 on the lipid II binding affinity.
Organic & Biomolecular Chemistry,
13(21), 5997-6009.
https://doi.org/10.1039/c5ob00336a Koopmans, T., Wood, T. M.
, 'T Hart, P., Kleijn, L. H. J., Hendrickx, A. P. A., Willems, R. J. L.
, Breukink, E., & Martin, N. I. (2015).
Semisynthetic Lipopeptides Derived from Nisin Display Antibacterial Activity and Lipid II Binding on Par with That of the Parent Compound.
Journal of the American Chemical Society,
137(29), 9382-9389.
https://doi.org/10.1021/jacs.5b04501 Gray, A. N., Egan, A. J. F.
, van 't Veer, I., Verheul, J., Colavin, A., Koumoutsi, A., Biboy, J.
, Altelaar, A. F. M., Damen, M. J., Huang, K. C., Simorre, J-P.
, Breukink, E., den Blaauwen, T., Typas, A., Gross, C. A., & Vollmer, W. (2015).
Coordination of peptidoglycan synthesis and outer membrane constriction during Escherichia coli cell division.
eLife,
4.
https://doi.org/10.7554/eLife.071182014
Wetenschappelijke publicaties
Scheidelaar, S., Koorengevel, M., Swainsbury, D.
, Meeldijk, H., Breukink, E., Jones, M., van Grondelle, R.
, & Killian, A. (2014).
Detergent-Free Extraction of Membrane Proteins into Native Nanodiscs. Application to the Reaction center of Rhodobacter Sphaeroides.
Biophysical Journal,
106(2), 90a.
https://doi.org/10.1016/j.bpj.2013.11.572 Harmsen, R. A. G., Ghalit, N.
, Kemmink, J., Breukink, E., Liskamp, R. M. J., & Rijkers, D. T. S. (2014).
A conformationally constrained fused tricyclic nisin AB-ring system mimic toward an improved pyrophosphate binder of lipid II.
Tetrahedron,
70(42), 7691-7699.
https://doi.org/10.1016/j.tet.2014.06.023Van Vught, R., Pieters, R. J., & Breukink, E. (2014).
Site-specific functionalization of proteins and their applications to therapeutic antibodies.
Computational and Structural Biotechnology Journal,
9(14), [e201402001].
https://doi.org/10.5936/csbj.201402001 Egan, A. J. F., Jean, N. L., Koumoutsi, A., Bougault, C. M., Biboy, J., Sassine, J., Solovyova, A. S.
, Breukink, E., Typas, A., Vollmer, W., & Simorre, J. P. (2014).
Outer-membrane lipoprotein LpoB spans the periplasm to stimulate the peptidoglycan synthase PBP1B.
Proceedings of the National Academy of Sciences of the United States of America,
111(22), 8197-8202.
https://doi.org/10.1073/pnas.1400376111Slootweg, J. C., Peters, N.
, Quarles Van Ufford, H. C., Breukink, E., Liskamp, R. M. J., & Rijkers, D. T. S. (2014).
Semi-synthesis of biologically active nisin hybrids composed of the native lanthionine ABC-fragment and a cross-stapled synthetic DE-fragment.
Bioorganic & Medicinal Chemistry,
22(19), 5345-5353.
https://doi.org/10.1016/j.bmc.2014.07.046 Mohammadi, T., Sijbrandi, R., Lutters, M., Verheul, J.
, Martin, N. I., den Blaauwen, T.
, de Kruijff, B., & Breukink, E. (2014).
Specificity of the transport of lipid II by FtsW in Escherichia coli.
Journal of Biological Chemistry,
289(21), 14707-14718.
https://doi.org/10.1074/jbc.M114.557371 Populariserende publicaties
Slootweg, J. C., Van Schaik, T. B.
, Van Ufford, H. C. Q., Breukink, E., Liskamp, R. M. J., & Rijkers, D. S. (2014).
Synthese en toepassing van een lipofiel aminozuurderivaat voor het verbeteren van de membraanaffiniteit van antimicrobiële peptiden.
Pharmaceutisch Weekblad,
149(43), 174-179.
https://www.scopus.com/record/display.uri?eid=2-s2.0-84949966040&origin=inward&txGid=54b908454fc67400cac49c3a0f33d69c 2013
Wetenschappelijke publicaties
Scheidelaar, S., Koorengevel, M. C., Meeldijk, H., Breukink, E., & Killian, A. (2013).
Towards Detergent Free Solubilization of Membrane Proteins into Nanodiscs: A Biophysical Study on the Interaction between Styrene Maleic Acid (SMA) Copolymers and Synthetic Phospholipid Vesicles.
Biophysical Journal,
104(2).
https://doi.org/10.1016/j.bpj.2012.11.3313 Imran, M., Revol-Junelles, A-M.
, Diepeveen-de Bruin, M., Paris, C.
, Breukink, E. J., & Desobry, S. (2013).
Fluorescent labeling of nisin Z and assessment of anti-listerial action.
Journal of Microbiological Methods,
95(2), 107-113.
https://doi.org/10.1016/j.mimet.2013.07.009 Slootweg, J. C., van Schaik, T. B.
, Quarles van Ufford, H. C., Breukink, E. J., Liskamp, R. M. J., & Rijkers, D. T. S. (2013).
Improving the biological activity of the antimicrobial peptide anoplin by membrane anchoring through a lipophilic amino acid derivative.
Bioorganic & Medicinal Chemistry Letters,
23, 3749-3752.
https://doi.org/10.1016/j.bmcl.2013.05.002 Overige resultaten
Hoekstra, J., Jenneskens, L. W., Geus, J. W., & Breukink, E. J. (2013). Amorphous Carbon Supported Nanoparticles Comprising Oxides Of Lanthanides And Method For Preparing Them. (Patent No. WO2013/144879).
2012
Wetenschappelijke publicaties
Banzhaf, M., van den Berg van Saparoea, B., Terrak, M., Fraipont, C., Egan, A., Philippe, J., Zapun, A.
, Breukink, E., Nguyen-Distèche, M., den Blaauwen, T., & Vollmer, W. (2012).
Cooperativity of peptidoglycan synthases active in bacterial cell elongation.
Molecular Microbiology,
85(1), 179-194.
https://doi.org/10.1111/j.1365-2958.2012.08103.xNikolaidis, I., Izoré, T., Job, V., Thielens, N.
, Breukink, E., & Dessen, A. (2012).
Calcium-dependent complex formation between PBP2 and lytic transglycosylase SltB1 of Pseudomonas aeruginosa.
Microbial Drug Resistance,
18(3), 298-305.
https://doi.org/10.1089/mdr.2012.0006 Helassa, N., Vollmer, W.
, Breukink, E., Vernet, T., & Zapun, A. (2012).
The membrane anchor of penicillin-binding protein PBP2a from Streptococcus pneumoniae influences peptidoglycan chain length.
The FEBS journal,
279(11), 2071-81.
https://doi.org/10.1111/j.1742-4658.2012.08592.xPaiva, A. D., Irving, N.
, Breukink, E., & Mantovani, H. C. (2012).
Interaction with lipid II induces conformational changes in bovicin HC5 structure.
Antimicrobial Agents and Chemotherapy,
56(9), 4586-4593.
https://doi.org/10.1128/AAC.00295-12 Paiva, A. D., de Oliveira, M. D., de Paula, S. O., Baracat-Pereira, M. C.
, Breukink, E., & Mantovani, H. C. (2012).
Toxicity of bovicin HC5 against mammalian cell lines and the role of cholesterol in bacteriocin activity.
Microbiology,
158(Pt 11), 2851-8.
https://doi.org/10.1099/mic.0.062190-0 Broekgaarden, M., Van Vught, R., De Kroon, A. I. P. M., Breukink, E., Van Gulik, T. M.
, & Heger, M. (2012).
Assessment of in vitro efficiency of multi-targeting liposomes containing zinc-phthalocyanine for photodynamic therapy.
Lasers in Surgery and Medicine,
44, 38-39.
https://doi.org/10.1002/lsm.22023 Te Welscher, Y. M., Van Leeuwen, M. R., De Kruijff, B., Dijksterhuis, J.
, & Breukink, E. (2012).
Polyene antibiotic that inhibits membrane transport proteins.
Proceedings of the National Academy of Sciences of the United States of America,
109(28), 11156-11159.
https://doi.org/10.1073/pnas.1203375109 Mulder, M. P. C., Fodran, P.
, Kemmink, J., Breukink, E. J., Kruijtzer, J. A. W., Minnaard, A. J.
, & Liskamp, R. M. J. (2012).
Mutual influence of backbone proline substitution and lipophilic tail character on the biological activity of simplified analogues of caspofungin.
Organic and Biomolecular Chemistry,
10(37), 7491-7502.
https://doi.org/10.1039/c2ob25951f Mulder, M. P. C., Fodran, P., Kemmink, J., Breukink, E. J., Kruijtzer, J. A. W., Minnaard, A. J., & Liskamp, R. M. J. (2012). Mutual influence of backbone proline substitution and lipopholic tail character on the biological activity of simplified analogues of caspofungin. Organic & Biomolecular Chemistry, 10, 7491-7502.
2011
Wetenschappelijke publicaties
Derouaux, A., Turk, S.
, Olrichs, N. K., Gobec, S.
, Breukink, E., Amoroso, A., Offant, J., Bostock, J., Mariner, K., Chopra, I., Vernet, T., Zervosen, A., Joris, B., Frère, J-M., Nguyen-Distèche, M., & Terrak, M. (2011).
Small molecule inhibitors of peptidoglycan synthesis targeting the lipid II precursor.
Biochemical Pharmacology,
81(9), 1098-105.
https://doi.org/10.1016/j.bcp.2011.02.008Paiva, A. D., Breukink, E., & Mantovani, H. C. (2011).
Role of lipid II and membrane thickness in the mechanism of action of the lantibiotic bovicin HC5.
Antimicrobial Agents and Chemotherapy,
55(11), 5284-5293.
https://doi.org/10.1128/AAC.00638-11 Olrichs, N. K., Aarsman, M. E. G., Verheul, J.
, Arnusch, C. J., Martin, N. I., Hervé, M., Vollmer, W.
, de Kruijff, B., Breukink, E., & den Blaauwen, T. (2011).
A Novel in vivo Cell-Wall Labeling Approach Sheds New Light on Peptidoglycan Synthesis in Escherichia coli.
ChemBioChem,
12(7), 1124-1133.
https://doi.org/10.1002/cbic.201000552 Mohammadi, T., Van Dam, V., Sijbrandi, R., Vernet, T., Zapun, A., Bouhss, A.
, Diepeveen-De Bruin, M., Nguyen-Distéche, M.
, De Kruijff, B., & Breukink, E. (2011).
Identification of FtsW as a transporter of lipid-linked cell wall precursors across the membrane.
EMBO Journal,
30(8), 1425-1432.
https://doi.org/10.1038/emboj.2011.61 Olrichs, N. K., Aarsman, M. E. G., Verheul, J., Arnusch, C. J., Martin, N. I., Herve, M., Vollmer, W., de Kruijff, B., Breukink, E. J., & den Blaauwen, T. A. (2011). A novel in vivo cell-wall labelling approach sheds new light on peptidoglycan synthesis in Escherichia coli. ChemBioChem, 12, 1124-1133.
2010
Wetenschappelijke publicaties
Typas, A., Banzhaf, M., van den Berg van Saparoea, B., Verheul, J., Biboy, J., Nichols, R. J., Zietek, M., Beilharz, K., Kannenberg, K., von Rechenberg, M.
, Breukink, E., den Blaauwen, T., Gross, C. A., & Vollmer, W. (2010).
Regulation of peptidoglycan synthesis by outer-membrane proteins.
Cell,
143(7), 1097-109.
https://doi.org/10.1016/j.cell.2010.11.038Offant, J., Terrak, M., Derouaux, A.
, Breukink, E. J., Nguyen-Distèche, M., Zapun, A., & Vernet, T. (2010).
Optimization of conditions for the glycosyltransferase activity of penicillin-binding protein 1a from Thermotoga maritima.
The FEBS journal,
277(20), 4290-4298.
https://doi.org/10.1111/j.1742-4658.2010.07817.x2009
Wetenschappelijke publicaties
Pieters, R. J., Arnusch, C. J., & Breukink, E. J. (2009). Membrane permeabilization by multivalent anti-microbial peptides. Proteins peptide letters, 16, 736-742.
2008
Wetenschappelijke publicaties
Kramer, N. E., Hasper, H. E., van den Bogaard, P. T. C., Morath, S.
, de Kruijff, B., Hartung, T., Smid, E. J.
, Breukink, E. J., Kok, J., & Kuipers, O. P. (2008).
Increased D-alanylation of lipoteichoic acid and a thickened septum are main determinants in the nisin resistance mechanism of Lactococcus lactis.
Microbiology,
154, 1755-1762.
https://dspace.library.uu.nl/bitstream/handle/1874/33262/10Kramer-%2520Increased.pdf?sequence=1Terrak, M., Sauvage, E., Derouaux, A., Dehareng, D., Bouhss, A.
, Breukink, E. J., Jeanjean, S., & Nguyen-Distèche, M. (2008).
Importance of the conserved residues in the peptidoglycan glycosyltransferase module of the class A penicillin-binding protein 1b of Escherichia coli.
Journal of Biological Chemistry,
283(42), 28464-28470.
https://dspace.library.uu.nl/bitstream/handle/1874/33206/14Terrak-Importance.pdf?sequence=1Derouaux, A., Wolf, B., Fraipont, C., Breukink, E. J., Nguyen-Distèche, M., & Terrak, M. (2008). The monofunctional glycosyltransferase of Escherichia coli localizes to the cell division site and interacts with the penicillin-binding protein 3 (PBP3), FtsW and FtsN. Journal of Bacteriology, 190(5), 1831-1834.
te Welscher, Y. M., ten Napel, H. H., Masià Balagué, M., Souza, C. M., Riezman, H., de Kruijff, B., & Breukink, E. J. (2008). Natamycin blocks fungal growth by binding specifically to ergosterol without permeabilizing the membrane. Journal of Biological Chemistry, 283(10), 6393-6401.
Arnusch, C. J., Bonvin, A. M. J. J., Verel, A. M., Jansen, W. T. M.
, Liskamp, R. M. J., de Kruijff, B., Pieters, R. J., & Breukink, E. J. (2008).
The vancomycin-nisin(1-12) hybrid restores activity against vancomycin resistant enterococci.
Biochemistry,
47(48), 12661-12663.
https://doi.org/10.1021/bi801597b Parisot, J., Carey, S., Breukink, E. J., Chan, W. C., Narbad, A., & Bonev, B. B. (2008). Molecular mechanism of target recognition by Subtilin, a class I Lanthionine antibiotic. Antimicrobial Agents and Chemotherapy, 52(2), 612-618.
2007
Wetenschappelijke publicaties
Arnusch, C. J., Branderhorst, H.
, De Kruijff, B., Liskamp, R. M. J., Breukink, E., & Pieters, R. J. (2007).
Enhanced membrane pore formation by multimeric/oligomeric antimicrobial peptides.
Biochemistry,
46(46), 13437-13442.
https://doi.org/10.1021/bi7015553 Hasegawa, M., Kawasaki, A., Yang, K., Fujimoto, Y., Masumoto, J., Breukink, E. J., Nuñez, G., Fukase, K., & Inohara, N. (2007). A role of lipophilic peptidoglycan-related molecules in induction of Nod1-mediated immune responses. Journal of Biological Chemistry, 282(16), 11757-11764.
Ghalit, N., Reichwein, J. F.
, Hilbers, H. W., Breukink, E. J., Rijkers, D. T. S., & Liskamp, R. M. J. (2007).
Synthesis of bicyclic alkene/alkane-bridged nisin mimics by ring-closing metathesis and their biochemical evaluation as Lipid II binders: toward the design of potential novel antibiotics.
ChemBioChem,
8, 1540-1554.
https://dspace.library.uu.nl/bitstream/handle/1874/26104/Ghalit-Synthesis%2520of%2520bicyclic.pdf?sequence=12006
Wetenschappelijke publicaties
Ganchev, D. N., Hasper, H. E.
, Breukink, E., & De Kruijff, B. (2006).
Size and orientation of the lipid II headgroup as revealed by AFM imaging.
Biochemistry,
45(19), 6195-6202.
https://doi.org/10.1021/bi051913eHsu, S. T., Breukink, E. J., & Kaptein, R. (2006). Structural motifs of lipid II-binding lantibiotics as a blueprint for novel antibiotics. Anti-Infective Agents in Medicinal Chemistry, 5(3), 245-254.
Populariserende publicaties
Breukink, E. (2006). Nieuw wapen tegen resistentie. Pharmaceutisch Weekblad, 141(26), 886-887.
2004
Wetenschappelijke publicaties
Hasper, H. E.
, De Kruijff, B., & Breukink, E. (2004).
Assembly and stability of nisin-Lipid II pores.
Biochemistry,
43(36), 11567-11575.
https://doi.org/10.1021/bi049476bHsu, S-T. D.
, Breukink, E., Tischenko, E.
, Lutters, M. A. G., De Kruijff, B., Kaptein, R., Bonvin, A. M. J. J., & Van Nuland, N. A. J. (2004).
The nisin-lipid II complex reveals a pyrophosphate cage that provides a blueprint for novel antibiotics.
Nature Structural and Molecular Biology,
11(10), 963-967.
https://doi.org/10.1038/nsmb830Bonev, B. B.
, Breukink, E. J., Swiezewska, E.
, de Kruijff, B., & Watts, A. (2004).
Targeting extracellular pyrophosphates underpins the high selectivity of nisin.
FASEB Journal,
18, 1862-1869.
https://dspace.library.uu.nl/bitstream/handle/1874/14608/Bonev-Targeting%2520extracellular.pdf?sequence=12003
Wetenschappelijke publicaties
Hsu, S-T. D.
, Breukink, E., Bierbaum, G., Sahl, H-G.
, De Kruijff, B., Kaptein, R., Van Nuland, N. A. J.
, & Bonvin, A. M. J. J. (2003).
NMR study of mersacidin and lipid II interaction in dodecylphosphocholine micelles: Conformational changes are a key to antimicrobial activity.
Journal of Biological Chemistry,
278(15), 13110-13117.
https://doi.org/10.1074/jbc.M2111442002002
Wetenschappelijke publicaties
Wills, M., Morris, D.
, Breukink, E., Bonnici, P. J.
, & Heck, A. J. R. (2002).
Stereocontrolled synthesis of angularly fused tricyclic ring systems by means of 1-metalla-1,3,5-hexatrienes (M = Cr, W).
Chemistry - A European Journal,
8(4), 910-916.
https://doi.org/10.1002/1521-3765(20020215)8:4<910::AID-CHEM910>3.0.CO;2-THsu, S.
, Breukink, E. J., de Kruijff, B., Kaptein, R., Bonvin, A. M. J. J., & van Nuland, N. A. J. (2002).
Mapping the targeted membrane pore formation mechanism by solution NMR: the nisin Z and lipid II interaction in SDS micelles.
Biochemistry,
41, 7670-7676.
https://doi.org/10.1021/bi025679t2001
Wetenschappelijke publicaties
Van Kraaij, C.
, Breukink, E., Rollema, H. S., Bongers, R. S., Kosters, H. A.
, De Kruijff, B., & Kuipers, O. P. (2001).
Engineering a disulfide bond and free thiols in the lantibiotic nisin Z.
European Journal of Biochemistry,
267(3), 901-909.
https://doi.org/10.1046/j.1432-1327.2000.01075.x2000
Wetenschappelijke publicaties
Breukink, E., Ganz, P.
, De Kruijff, B., & Seelig, J. (2000).
Binding of nisin Z to bilayer vesicles as determined with isothermal titration calorimetry.
Biochemistry,
39(33), 10247-10254.
https://doi.org/10.1021/bi000915q 1999
Wetenschappelijke publicaties
Lins, L., Ducarme, P.
, Breukink, E., & Brasseur, R. (1999).
Computational study of nisin interaction with model membrane.
Biochimica et Biophysica Acta - Biomembranes,
1420(1-2), 111-120.
https://doi.org/10.1016/S0005-2736(99)00090-5Breukink, E., Wiedemann, I., Van Kraaij, C., Kuipers, O. P., Sahl, H-G.
, & De Kruijff, B. (1999).
Use of the cell wail precursor lipid II by a pore-forming peptide antibiotic.
Science,
286(5448), 2361-2364.
https://doi.org/10.1126/science.286.5448.2361 1998
Wetenschappelijke publicaties
Van Kraaij, C.
, Breukink, E., Noordermeer, M. A., Demel, R. A., Siezen, R. J., Kuipers, O. P.
, & De Kruijff, B. (1998).
Pore formation by nisin involves translocation of its C-terminal part across the membrane.
Biochemistry,
37(46), 16033-16040.
https://doi.org/10.1021/bi980931bBreukink, E., Van Kraaij, C., Van Dalen, A., Demel, R. A., Siezen, R. J.
, De Kruijff, B., & Kuipers, O. P. (1998).
The orientation of nisin in membranes.
Biochemistry,
37(22), 8153-8162.
https://doi.org/10.1021/bi972797l 1997
Wetenschappelijke publicaties
Van Kraaij, C., Breukink, E., Rollema, H. S., Siezen, R. J., Demel, R. A., De Kruijff, B., & Kuipers, O. P. (1997). Influence of charge differences in the C-terminal part of nisin on antimicrobial activity and signaling capacity. European Journal of Biochemistry, 247(1), 114-120.
Breukink, E., Van Kraaij, C., Demel, R. A., Siezen, R. J., Kuipers, O. P.
, & De Kruijff, B. (1997).
The C-terminal region of nisin is responsible for the initial interaction of nisin with the target membrane.
Biochemistry,
36(23), 6968-6976.
https://doi.org/10.1021/bi970008u Den Blaauwen, T., De Wit, J. G., Gosker, H., Van Der Does, C.
, Breukink, E-J., De Leij, L., & Driessen, A. J. M. (1997).
Inhibition of preprotein translocation and reversion of the membrane inserted state of SecA by a carboxyl terminus binding MAb.
Biochemistry,
36(30), 9159-9168.
https://doi.org/10.1021/bi970344a1996
Wetenschappelijke publicaties
Van Raalte, A. L. J., Demel, R. A., Verberkmoes, G., Breukink, E., Keller, R. C. A., & De Kruijff, B. (1996). Influence of the signal sequence and chaperone SecB on the interaction between precursor protein prePhoE and phospholipids. European Journal of Biochemistry, 235(1-2), 207-214.
1995
Wetenschappelijke publicaties
Breukink, E., Nouwen, N., Van Raalte, A., Mizushima, S., Tommassen, J.
, & De Kruijff, B. (1995).
The C terminus of SecA is involved in both lipid binding and SecB binding.
Journal of Biological Chemistry,
270(14), 7902-7907.
https://doi.org/10.1074/jbc.270.14.7902 1994
Wetenschappelijke publicaties
Kusters, R., Breukink, E., Gallusser, A., Kuhn, A., & De Kruijff, B. (1994). A dual role for phosphatidylglycerol in protein translocation across the Escherichia coli inner membrane. Journal of Biological Chemistry, 269(2), 1560-1563.
1993
Wetenschappelijke publicaties
Van der Wolk, J., Klose, M., Breukink, E., Demel, R. A., De Kruijff, B., Freudl, R., & Driessen, A. J. M. (1993). Characterization of a Bacillus subtilis SecA mutant protein deficient in translocation ATPase and release from the membrane. Molecular Microbiology, 8(1), 31-42.
Breukink, E., Keller, R. C. A.
, & De Kruijff, B. (1993).
Nucleotide and negatively charged lipid-dependent vesicle aggregation caused by SecA. Evidence that SecA contains two lipid-binding sites.
FEBS Letters,
331(1-2), 19-24.
https://doi.org/10.1016/0014-5793(93)80289-7 1992
Wetenschappelijke publicaties
Kruijff, B. D., Breukink, E., Demel, R. A., T Hof, R. V., Jongh, H. H. J. D., Jordi, W., Keller, R. C. A.
, Killian, J. A., Kroon, A. I. P. M. D., Kusters, R., & Pilon, M. (1992).
Lipid involvement in protein translocation.
New Comprehensive Biochemistry,
22(C), 85-101.
https://doi.org/10.1016/S0167-7306(08)60085-X Breukink, E., Demel, R. A., De Korte-Kool, G., & De Kruijff, B. (1992). SecA insertion into phospholipids is stimulated by negatively charged lipids and inhibited by ATP: A monolayer study. Biochemistry, 31(4), 1119-1124.
Breukink, E., Kusters, R., & De Kruijff, B. (1992). In-vitro studies on the folding characteristics of the Escherichia coli precursor protein prePhoE. Evidence that SecB prevents the precursor from aggregating by forming A functional complex. European Journal of Biochemistry, 208(2), 419-425.
1989
Wetenschappelijke publicaties
Kusters, R., de Vrije, T., Breukink, E., & De Kruijff, B. (1989). SecB protein stabilizes a translocation-competent state of purified prePhoE protein. Journal of Biological Chemistry, 264(35), 20827-20830.