prof. dr. A.S. (Anna) Akhmanova
a.akhmanova@uu.nl
Gegenereerd op 2018-09-22 13:49:59


Leeropdracht
Celbiologie
Benoemingsdatum 01-01-2011
Oratiedatum 01-02-2012
Profiel

Mijn groep bestudeert de organisatie en transportprocessen, die bijdragen aan celpolarisatie, differentiatie, ontwikkeling van gewervelde dieren en ziekten bij de mens. We zijn geïnteresseerd in het begrijpen, op systeemniveau, van hoe verschillende aspecten van celarchitectuur worden gecoördineerd.

De belangrijkste focus van ons onderzoek is het microtubuli celskelet. In ons onderzoek combineren we hoge-resolutie microscopie van levende cellen en kwantitatieve analyses van de dynamica van celskeletfilamenten, metingen van de dynamica van eiwitten, in vitro reconstitutie van dynamische cytoskelet-gebaseerde processen en verschillende methoden voor identificatie van eiwit-eiwit interacties, zoals massa spectrometrie.

Naast gekweekte cellen gebruiken we muis knock-out technologieën en verbinden we de genetische modificatie (zoals GFP knock-in technologie) met de imaging in levende cellen en weefsels. In samenwerking met wiskundigen werken we aan de ontwikkeling van geautomatiseerde analyse en modellering van cytoskeletdynamica en het membraantransport.

Microtubuli-gebaseerde processen zijn essentieel voor celdeling, het normaal functioneren van grote cellen zoals neuronen en voor intracellulair transport van pathogenen; daarom zijn onze studies relevant voor het bestrijden van abnormale celproliferatie, neurodegeneratie en infectieziekten.

  • Lid van de European Molecular Biology Organization (EMBO)
  • Lid van de Koninklijke Nederlandse Akademie van Wetenschappen (KNAW)
  • NWO Spinozapremie 2018
  • ERC Synergy-beurs 2013 (samen met Dr. Marileen Dogterom, TU Delft)
  • ALW Vernieuwingsimpuls VICI 2007
  • NWO Vernieuwingsimpuls VIDI 2001

Betrokken bij de opleiding(en)
Gegenereerd op 2018-09-22 13:49:59
Curriculum vitae

Anna Akhmanova studeerde biochemie en moleculaire biologie aan de Staatsuniversiteit van Moskou. Ze promoveerde in 1997 aan de Radboud Universiteit. Ze werkte als postdoc bij de afdeling Microbiologie en Evolutionaire Biologie aan de Radboud Universiteit en bij de afdeling Celbiologie in het Erasmus Medisch Centrum in Rotterdam. In 2001 startte ze haar eigen onderzoeksgroep bij de afdeling Celbiologie van het Erasmus Medisch Centrum in Rotterdam. Sinds 2011 is Anna Akhmanova hoogleraar celbiologie aan de Universiteit Utrecht.

Gegenereerd op 2018-09-22 13:49:59

Selected Publications

2018

Aher A and Akhmanova A. Tipping microtubule dynamics, one protofilament at a time. Curr Opin Cell Biol, 2018; 50: 86-93.

Martin M and Akhmanova A. Coming into Focus: Mechanisms of Microtubule Minus-End Organization. Trends Cell Biol, 2018.

Martin M, Veloso A, Wu J, Katrukha EA, and Akhmanova A. Control of endothelial cell polarity and sprouting angiogenesis by non-centrosomal microtubules. Elife, 2018; 7.

Jiang K, Faltova L, Hua S, Capitani G, Prota AE, Landgraf C, Volkmer R, Kammerer RA, Steinmetz MO, and Akhmanova A. Structural Basis of Formation of the Microtubule Minus-End-Regulating CAMSAP-Katanin Complex. Structure, 2018; 26: 375-382 e4.

 

2017

Akhmanova A and Maiato H. Closing the tubulin detyrosination cycle. Science, 2017; 358: 1381-1382.

Atherton J, Jiang K, Stangier MM, Luo Y, Hua S, Houben K, van Hooff JJE, Joseph AP, Scarabelli G, Grant BJ, Roberts AJ, Topf M, Steinmetz MO, Baldus M, Moores CA, and Akhmanova A. A structural model for microtubule minus-end recognition and protection by CAMSAP proteins. Nat Struct Mol Biol, 2017; 24: 931-943.

Jost M, Chen Y, Gilbert LA, Horlbeck MA, Krenning L, Menchon G, Rai A, Cho MY, Stern JJ, Prota AE, Kampmann M, Akhmanova A, Steinmetz MO, Tanenbaum ME, and Weissman JS. Combined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent. Mol Cell, 2017; 68: 210-223 e6.

Yang C, Wu J, de Heus C, Grigoriev I, Liv N, Yao Y, Smal I, Meijering E, Klumperman J, Qi RZ, and Akhmanova A. EB1 and EB3 regulate microtubule minus end organization and Golgi morphology. J Cell Biol, 2017; 216: 3179-3198.

Schmidt R, Fielmich LE, Grigoriev I, Katrukha EA, Akhmanova A, and van den Heuvel S. Two populations of cytoplasmic dynein contribute to spindle positioning in C. elegans embryos. J Cell Biol, 2017; 216: 2777-2793.

Wu J and Akhmanova A. Microtubule-Organizing Centers. Annu Rev Cell Dev Biol, 2017; 33: 51-75.

Kumar A, Manatschal C, Rai A, Grigoriev I, Degen MS, Jaussi R, Kretzschmar I, Prota AE, Volkmer R, Kammerer RA, Akhmanova A, and Steinmetz MO. Short Linear Sequence Motif LxxPTPh Targets Diverse Proteins to Growing Microtubule Ends. Structure, 2017; 25: 924-932 e4.

Noordstra I and Akhmanova A. Linking cortical microtubule attachment and exocytosis. F1000Res, 2017; 6: 469.

Jiang K, Rezabkova L, Hua S, Liu Q, Capitani G, Altelaar AFM, Heck AJR, Kammerer RA, Steinmetz MO, and Akhmanova A. Microtubule minus-end regulation at spindle poles by an ASPM-katanin complex. Nat Cell Biol, 2017; 19: 480-492.

Gumy LF, Katrukha EA, Grigoriev I, Jaarsma D, Kapitein LC, Akhmanova A, and Hoogenraad CC. MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus KIF5-Dependent Cargo Transport in Sensory Neurons. Neuron, 2017; 94: 347-362 e7.

van Riel WE, Rai A, Bianchi S, Katrukha EA, Liu Q, Heck AJ, Hoogenraad CC, Steinmetz MO, Kapitein LC, and Akhmanova A. Kinesin-4 KIF21B is a potent microtubule pausing factor. Elife, 2017; 6.

Bouchet BP and Akhmanova A. Microtubules in 3D cell motility. J Cell Sci, 2017; 130: 39-50.

Au FK, Jia Y, Jiang K, Grigoriev I, Hau BK, Shen Y, Du S, Akhmanova A, and Qi RZ. GAS2L1 Is a Centriole-Associated Protein Required for Centrosome Dynamics and Disjunction. Dev Cell, 2017; 40: 81-94.

 

2016

Bouchet BP, Noordstra I, van Amersfoort M, Katrukha EA, Ammon YC, Ter Hoeve ND, Hodgson L, Dogterom M, Derksen PWB, and Akhmanova A. Mesenchymal Cell Invasion Requires Cooperative Regulation of Persistent Microtubule Growth by SLAIN2 and CLASP1. Dev Cell, 2016; 39: 708-723.

Noordstra I, Liu Q, Nijenhuis W, Hua S, Jiang K, Baars M, Remmelzwaal S, Martin M, Kapitein LC, and Akhmanova A. Control of apico-basal epithelial polarity by the microtubule minus-end-binding protein CAMSAP3 and spectraplakin ACF7. J Cell Sci, 2016; 129: 4278-4288.

Wu J, de Heus C, Liu Q, Bouchet BP, Noordstra I, Jiang K, Hua S, Martin M, Yang C, Grigoriev I, Katrukha EA, Altelaar AFM, Hoogenraad CC, Qi RZ, Klumperman J, and Akhmanova A. Molecular Pathway of Microtubule Organization at the Golgi Apparatus. Dev Cell, 2016; 39: 44-60.

Guesdon A, Bazile F, Buey RM, Mohan R, Monier S, Garcia RR, Angevin M, Heichette C, Wieneke R, Tampe R, Duchesne L, Akhmanova A, Steinmetz MO, and Chretien D. EB1 interacts with outwardly curved and straight regions of the microtubule lattice. Nat Cell Biol, 2016; 18: 1102-8.

Liu Q, Liu F, Yu KL, Tas R, Grigoriev I, Remmelzwaal S, Serra-Marques A, Kapitein LC, Heck AJ, and Akhmanova A. MICAL3 Flavoprotein Monooxygenase Forms a Complex with Centralspindlin and Regulates Cytokinesis. J Biol Chem, 2016; 291: 20617-29.

Bianchi S, van Riel WE, Kraatz SH, Olieric N, Frey D, Katrukha EA, Jaussi R, Missimer J, Grigoriev I, Olieric V, Benoit RM, Steinmetz MO, Akhmanova A, and Kammerer RA. Structural basis for misregulation of kinesin KIF21A autoinhibition by CFEOM1 disease mutations. Sci Rep, 2016; 6: 30668.

Bouchet BP, Gough RE, Ammon YC, van de Willige D, Post H, Jacquemet G, Altelaar AM, Heck AJ, Goult BT, and Akhmanova A. Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions. Elife, 2016; 5.

Doodhi H, Prota AE, Rodriguez-Garcia R, Xiao H, Custar DW, Bargsten K, Katrukha EA, Hilbert M, Hua S, Jiang K, Grigoriev I, Yang CH, Cox D, Horwitz SB, Kapitein LC, Akhmanova A, and Steinmetz MO. Termination of Protofilament Elongation by Eribulin Induces Lattice Defects that Promote Microtubule Catastrophes. Curr Biol, 2016; 26: 1713-1721.

Sharma A, Aher A, Dynes NJ, Frey D, Katrukha EA, Jaussi R, Grigoriev I, Croisier M, Kammerer RA, Akhmanova A, Gonczy P, and Steinmetz MO. Centriolar CPAP/SAS-4 Imparts Slow Processive Microtubule Growth. Dev Cell, 2016; 37: 362-376.

Akhmanova A and van den Heuvel S. Tipping the spindle into the right position. J Cell Biol, 2016; 213: 293-5.

van de Willige D, Hoogenraad CC, and Akhmanova A. Microtubule plus-end tracking proteins in neuronal development. Cell Mol Life Sci, 2016; 73: 2053-77.

Kuijpers M, van de Willige D, Freal A, Chazeau A, Franker MA, Hofenk J, Rodrigues RJ, Kapitein LC, Akhmanova A, Jaarsma D, and Hoogenraad CC. Dynein Regulator NDEL1 Controls Polarized Cargo Transport at the Axon Initial Segment. Neuron, 2016; 89: 461-71.

Hoogenraad CC and Akhmanova A. Bicaudal D Family of Motor Adaptors: Linking Dynein Motility to Cargo Binding. Trends Cell Biol, 2016; 26: 327-340.

 

2015

van Beuningen SFB, Will L, Harterink M, Chazeau A, van Battum EY, Frias CP, Franker MAM, Katrukha EA, Stucchi R, Vocking K, Antunes AT, Slenders L, Doulkeridou S, Sillevis Smitt P, Altelaar AFM, Post JA, Akhmanova A, Pasterkamp RJ, Kapitein LC, de Graaff E, and Hoogenraad CC. TRIM46 Controls Neuronal Polarity and Axon Specification by Driving the Formation of Parallel Microtubule Arrays. Neuron, 2015; 88: 1208-1226.

Akhmanova A and Steinmetz MO. Control of microtubule organization and dynamics: two ends in the limelight. Nat Rev Mol Cell Biol, 2015; 16: 711-26.

Akhmanova A and Hoogenraad CC. Microtubule minus-end-targeting proteins. Curr Biol, 2015; 25: R162-71.

 

2014

Yau KW, van Beuningen SF, Cunha-Ferreira I, Cloin BM, van Battum EY, Will L, Schatzle P, Tas RP, van Krugten J, Katrukha EA, Jiang K, Wulf PS, Mikhaylova M, Harterink M, Pasterkamp RJ, Akhmanova A, Kapitein LC, Hoogenraad CC (2014). Microtubule minus-end binding protein CAMSAP2 controls axon specification and dendrite development. Neuron, 82: 1058-73.

Schlager MA, Serra-Marques A, Grigoriev I, Gumy LF, Esteves da Silva M, Wulf PS, Akhmanova A, Hoogenraad CC (2014). Bicaudal d family adaptor proteins control the velocity of Dynein-based movements. Cell Rep, 8:1248-56.

Preciado Lopez M, Huber F, Grigoriev I, Steinmetz MO, Akhmanova A, Koenderink GH, Dogterom M. (2014). Actin-microtubule coordination at growing microtubule ends. Nat Commun, 5:4778.

Jiang K, Hua S, Mohan R, Grigoriev I, Yau KW, Liu Q, Katrukha EA, Altelaar AF, Heck AJ, Hoogenraad CC, Akhmanova A. 2014. Microtubule minus-end stabilization by polymerization-driven CAMSAP deposition. Dev Cell, 28:295-309.

Doodhi H, Katrukha EA, Kapitein LC, Akhmanova A (2014). Mechanical and geometrical constraints control Kinesin-based microtubule guidance. Curr Biol 24:322-8.

2013

van der Vaart B, van Riel WE, Doodhi H, Kevenaar JT, Katrukha EA, Gumy L, Bouchet BP, Grigoriev I, Spangler SA, Yu KL, Wulf PS, Wu J, Lansbergen G, van Battum EY, Pasterkamp RJ, Mimori-Kiyosue Y, Demmers J, Olieric N, Maly IV, Hoogenraad CC, and Akhmanova A (2013). CFEOM1-associated kinesin KIF21A is a cortical microtubule growth inhibitor. Dev Cell. 27: 145-60.

Mohan R, Katrukha EA, Doodhi H, Smal I, Meijering E, Kapitein LC, Steinmetz MO, and Akhmanova A (2013). End-binding proteins sensitize microtubules to the action of microtubule-targeting agents. Proc Natl Acad Sci U S A. 110: 8900-5.

Hu DJ, Baffet AD, Nayak T, Akhmanova A, Doye V, and Vallee RB (2013). Dynein recruitment to nuclear pores activates apical nuclear migration and mitotic entry in brain progenitor cells. Cell 154: 1300-13.

van Spronsen M, Mikhaylova M, Lipka J, Schlager MA, van den Heuvel DJ, Kuijpers M, Wulf PS, Keijzer N, Demmers J, Kapitein LC, Jaarsma D, Gerritsen HC, Akhmanova A, Hoogenraad CC (2013) TRAK/Milton Motor-Adaptor Proteins Steer Mitochondrial Trafficking to Axons and Dendrites. Neuron 77: 485-502.


2012

van der Vaart B, Franker MA, Kuijpers M, Hua S, Bouchet BP, Jiang K, Grigoriev I, Hoogenraad CC, Akhmanova A (2012) Microtubule plus-end tracking proteins SLAIN1/2 and ch-TOG promote axonal development. J Neurosci 32: 14722-14728.

Splinter D, Razafsky DS, Schlager MA, Serra-Marques A, Grigoriev I, Demmers J, Keijzer N, Jiang K, Poser I, Hyman AA, Hoogenraad CC, King SJ, Akhmanova A (2012) BICD2, dynactin, and LIS1 cooperate in regulating dynein recruitment to cellular structures. Mol Biol Cell 23: 4226-4241.

Ratheesh A, Gomez GA, Priya R, Verma S, Kovacs EM, Jiang K, Brown NH, Akhmanova A, Stehbens SJ, Yap AS (2012) Centralspindlin and alpha-catenin regulate Rho signalling at the epithelial zonula adherens. Nat Cell Biol 14: 818-828.

Jiang K, Toedt G, Montenegro Gouveia S, Davey NE, Hua S, van der Vaart B, Grigoriev I, Larsen J, Pedersen LB, Bezstarosti K, Lince-Faria M, Demmers J, Steinmetz MO, Gibson TJ, Akhmanova A (2012) A Proteome-wide screen for mammalian SxIP motif-containing microtubule plus-end tracking proteins. Curr Biol 22: 1800-1807.

2011

van der Vaart B, Manatschal C, Grigoriev I, Olieric V, Gouveia SM, Bjelic S, Demmers J, Vorobjev I, Hoogenraad CC, Steinmetz MO, Akhmanova A (2011) SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase. J Cell Biol 193: 1083-1099.

Kovacs EM, Verma S, Ali RG, Ratheesh A, Hamilton NA, Akhmanova A, Yap AS (2011) N-WASP regulates the epithelial junctional actin cytoskeleton through a non-canonical post-nucleation pathway. Nat Cell Biol 13: 934-943.

Jiang K, Akhmanova A (2011) Microtubule tip-interacting proteins: a view from both ends. Curr Opin Cell Biol 23: 94-101.

Grigoriev I, Yu KL, Martinez-Sanchez E, Serra-Marques A, Smal I, Meijering E, Demmers J, Peranen J, Pasterkamp RJ, van der Sluijs P, Hoogenraad CC, Akhmanova A (2011) Rab6, Rab8, and MICAL3 cooperate in controlling docking and fusion of exocytotic carriers. Curr Biol 21: 967-974.

Akhmanova A, Dogterom M (2011) Kinesins lead aging microtubules to catastrophe. Cell 147: 966-968.

2010

Splinter D, Tanenbaum ME, Lindqvist A, Jaarsma D, Flotho A, Yu KL, Grigoriev I, Engelsma D, Haasdijk ED, Keijzer N, Demmers J, Fornerod M, Melchior F, Hoogenraad CC, Medema RH, Akhmanova A (2010) Bicaudal D2, dynein, and kinesin-1 associate with nuclear pore complexes and regulate centrosome and nuclear positioning during mitotic entry. PLoS Biol 8: e1000350.

Montenegro Gouveia S, Leslie K, Kapitein LC, Buey RM, Grigoriev I, Wagenbach M, Smal I, Meijering E, Hoogenraad CC, Wordeman L, Steinmetz MO, Akhmanova A (2010) In vitro reconstitution of the functional interplay between MCAK and EB3 at microtubule plus ends. Curr Biol 20: 1717-1722.

Gouveia SM, Akhmanova A (2010) Cell and molecular biology of microtubule plus end tracking proteins: end binding proteins and their partners. Int Rev Cell Mol Biol 285: 1-74.

Akhmanova A, Steinmetz MO (2010) Microtubule +TIPs at a glance. J Cell Sci 123: 3415-3419.

Akhmanova A, Hammer JA, 3rd (2010) Linking molecular motors to membrane cargo. Curr Opin Cell Biol 22: 479-487.

2009

Lomakin AJ, Semenova I, Zaliapin I, Kraikivski P, Nadezhdina E, Slepchenko BM, Akhmanova A, Rodionov V (2009) CLIP-170-dependent capture of membrane organelles by microtubules initiates minus-end directed transport. Dev Cell 17: 323-333.

Komarova Y, De Groot CO, Grigoriev I, Gouveia SM, Munteanu EL, Schober JM, Honnappa S, Buey RM, Hoogenraad CC, Dogterom M, Borisy GG, Steinmetz MO, Akhmanova A (2009) Mammalian end binding proteins control persistent microtubule growth. J Cell Biol 184: 691-706.

Honnappa S, Gouveia SM, Weisbrich A, Damberger FF, Bhavesh NS, Jawhari H, Grigoriev I, van Rijssel FJ, Buey RM, Lawera A, Jelesarov I, Winkler FK, Wuthrich K, Akhmanova A, Steinmetz MO (2009) An EB1-binding motif acts as a microtubule tip localization signal. Cell 138: 366-376.

2008

Grigoriev I, Gouveia SM, van der Vaart B, Demmers J, Smyth JT, Honnappa S, Splinter D, Steinmetz MO, Putney JW, Jr., Hoogenraad CC, Akhmanova A (2008) STIM1 is a MT-plus-end-tracking protein involved in remodeling of the ER. Curr Biol 18: 177-182.

Akhmanova A, Yap AS (2008) Organizing junctions at the cell-cell interface. Cell 135: 791-793.

Akhmanova A, Steinmetz MO (2008) Tracking the ends: a dynamic protein network controls the fate of microtubule tips. Nat Rev Mol Cell Biol 9: 309-322.

2007

Grigoriev I, Splinter D, Keijzer N, Wulf PS, Demmers J, Ohtsuka T, Modesti M, Maly IV, Grosveld F, Hoogenraad CC, Akhmanova A (2007) Rab6 regulates transport and targeting of exocytotic carriers. Dev Cell 13: 305-314.

2006

Lansbergen G, Grigoriev I, Mimori-Kiyosue Y, Ohtsuka T, Higa S, Kitajima I, Demmers J, Galjart N, Houtsmuller AB, Grosveld F, Akhmanova A (2006) CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta. Dev Cell 11: 21-32.

2005

Mimori-Kiyosue Y, Grigoriev I, Lansbergen G, Sasaki H, Matsui C, Severin F, Galjart N, Grosveld F, Vorobjev I, Tsukita S, Akhmanova A (2005) CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex. J Cell Biol 168: 141-153.

Akhmanova A, Mausset-Bonnefont AL, van Cappellen W, Keijzer N, Hoogenraad CC, Stepanova T, Drabek K, van der Wees J, Mommaas M, Onderwater J, van der Meulen H, Tanenbaum ME, Medema RH, Hoogerbrugge J, Vreeburg J, Uringa EJ, Grootegoed JA, Grosveld F, Galjart N (2005) The microtubule plus-end-tracking protein CLIP-170 associates with the spermatid manchette and is essential for spermatogenesis. Genes Dev 19: 2501-2515.

Akhmanova A, Hoogenraad CC (2005) Microtubule plus-end-tracking proteins: mechanisms and functions. Curr Opin Cell Biol 17: 47-54.

2004

Lansbergen G, Komarova Y, Modesti M, Wyman C, Hoogenraad CC, Goodson HV, Lemaitre RP, Drechsel DN, van Munster E, Gadella TW, Jr., Grosveld F, Galjart N, Borisy GG, Akhmanova A (2004) Conformational changes in CLIP-170 regulate its binding to microtubules and dynactin localization. J Cell Biol 166: 1003-1014.

 

 

^ naar boven
Gegenereerd op 2018-09-22 13:49:59
Nevenfuncties
  • Elife, Deputy Editor
  • PLoS Biology, Editorial Board Member
  • Journal of Cell Science, Advisory Board Member
  • Traffic, Editorial Board Member
  • BioArchitecture, Editorial Board Member
  • Membership in national and international grant panels
  • Faculty Member of the “FACULTY OF 1000 BIOLOGY” Cell Biology section
Gegenereerd op 2018-09-22 13:49:59
Volledige naam
prof. dr. A.S. Akhmanova Contactgegevens
Hugo R. Kruytgebouw

Padualaan 8
Kamer O503
3584 CH  UTRECHT

Telefoonnummer direct 030 253 2328
Fax 030 253 2837
Gegenereerd op 2018-09-22 13:49:59
Laatst bijgewerkt op 18-06-2018