dr. A.I.P.M. (Toon) de Kroon
Gegenereerd op 2018-09-22 13:15:38


Research in my group is aimed at understanding the principles that govern membrane lipid homeostasis and identifying the underlying sensor-effector modules. These topics are investigated in the reference eukaryote baker’s yeast (S. cerevisiae) using biochemical, genetic and chemical biological approaches, complemented by lipidome analysis. A novel regulatory mechanism maintaining the balance between bilayer and non-bilayer lipids was discovered that adapts lipid acyl chain composition in response to changes in lipid class composition and vice versa. Current research focuses on the identity and molecular mechanism of the gene products involved including the membrane sensor. Acyltransferases were found to determine acyl chain desaturation, providing new insight into the regulation of membrane fluidity and generating new tools for manipulating membrane lipid composition. Photoactivatable and clickable phospholipid probe molecules have been developed that were successfully used to discover new lipid-protein interactions in biological membranes. In flanking application-oriented research, we aim to improve the performance of yeast in production processes involving hydrophobic substances by engineering the membrane lipid composition.

Strategische thema's / focusgebieden
Gegenereerd op 2018-09-22 13:15:38
Curriculum vitae


Toon de Kroon received his PhD cum laude (1991) at Utrecht University (promotores prof. dr. Ben de Kruijff and prof. dr. Hans de Gier). After post-doctoral research at Stanford University with prof. dr. Harden McConnell funded by an NWO-stipend, he returned to Utrecht University, and joined the faculty of the Department of Chemistry in 1995. Research in the De Kroon group focuses on phospholipid-protein interactions and their functions in cell biology, and on regulatory mechanisms of membrane lipid homeostasis. He teaches courses in the bachelor programs of Chemistry and of Biomedical Sciences, and coordinates a course in the master program MCLS. He is a member of several education-related committees. He received an NWO “Jonge Chemici” fellowship and several CW-ECHO grants, has participated in EU-Networks, and (co-)authored over 80 peer-reviewed publications.


Gegenereerd op 2018-09-22 13:15:38
Alle publicaties
  2016 - Wetenschappelijke publicaties
Renne, Mike F, Bao, Xue, De Smet, Cedric H & de Kroon, Anton I P M (19-01-2016). Lipid Acyl Chain Remodeling in Yeast. Lipid Insights, 8 (Suppl 1), (pp. 33-40) (8 p.).
Van Raath, M. Ingmar, Weijer, Ruud, Nguyen, Gia Hung, Choi, Bernard, De Kroon, Anton I. & Heger, Michal (01-08-2016). Tranexamic acid-encapsulating thermosensitive liposomes for site-specific pharmaco-laser therapy of port wine stains. Journal of Biomedical Nanotechnology, 12 (8), (pp. 1617-1640) (24 p.).
  2015 - Wetenschappelijke publicaties
Furse, Samuel, Wienk, Hans, Boelens, Rolf, de Kroon, Anton I. P. M. & Killian, J. Antoinette (14-09-2015). E. coli MG1655 modulates its phospholipid composition through the cell cycle. FEBS Letters, 589 (19), (pp. 2726-2730) (5 p.).
Furse, Samuel & De Kroon, Anton I P M (19-05-2015). Phosphatidylcholine's functions beyond that of a membrane brick. Molecular Membrane Biology, 32 (4), (pp. 117-119) (3 p.).
  2013 - Wetenschappelijke publicaties
de Kroon, A.I.P.M., Rijken, P.J. & De Smet, C.H. (2013). Checks and balances in membrane phospholipid class and acyl chain homeostasis, the yeast perspective. Progress in Lipid Research, 52 (4), (pp. 374-394) (21 p.).
Broekgaarden, M., de Kroon, A.I.P.M., van Gulik, T.M. & Heger, M. (2013). Development and In Vitro Proof-of-Concept of Interstitially Targeted Zinc- Phthalocyanine Liposomes for Photodynamic Therapy. Current Medicinal Chemistry, 21 (3), (pp. 377-391) (15 p.).
Tavassoli, S., Chao, J.T., Young, B.P., Cox, R.C., Prinz, W.A., de Kroon, A.I.P.M. & Loewen, C.I.R. (2013). Plasma membraneā€”endoplasmic reticulum contact sites regulate phosphatidylcholine synthesis. EMBO Reports, 14, (pp. 434-440) (17 p.).
De Smet, C.H., Cox, R., Brouwers, J.F.H.M. & de Kroon, A.I.P.M. (2013). Yeast cells accumulate excess endogenous palmitate in phosphatidylcholine by acyl chain remodeling involving the phospholipase B Plb1p. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1831 (6), (pp. 1167-1176) (10 p.).
  2012 - Wetenschappelijke publicaties
Broekgaarden, Mans, Van Vught, Remko, De Kroon, Anton I.P.M., Breukink, Eefjan, Van Gulik, Thomas M. & Heger, Michal (01-03-2012). Assessment of in vitro efficiency of multi-targeting liposomes containing zinc-phthalocyanine for photodynamic therapy. Lasers in Surgery and Medicine, 44, (pp. 38-39) (2 p.).
De Smet, C.H., Vittone, E., Scherer, M., Houweling, M., Liebisch, G., Brouwers, J.F.H.M. & de Kroon, Toon (01-04-2012). The yeast acyltransferase Sct1p regulates fatty acid desaturation by competing with the desaturase Ole1p. Molecular Biology of the Cell, Apr; 23 ((7)), (pp. 1146-1156) (11 p.).
  2011 - Wetenschappelijke publicaties
Bilgin, M., Markgraf, D.F., Duchoslav, E., Knudsen, J., Jensen, O.N., de Kroon, A.I.P.M. & Ejsing, C.S. (2011). Quantitative profiling of PE, MMPE, DMPE, and PC lipid species by multiple precursor ion scanning: a tool for monitoring PE metabolism. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1811 (12), (pp. 1091-1089) (9 p.).
  2010 - Wetenschappelijke publicaties
van Meer, G. & de Kroon, A.I.P.M. (2010). Lipid map of the mammalian cell. Journal of Cell Science, 124, (pp. 5-8) (4 p.).
Gubbens, J. & de Kroon, A.I.P.M. (2010). Proteome-wide detection of phospholipid-protein interactions in mitochondria by photocrosslinking and click chemistry. Molecular BioSystems, 6 (10), (pp. 1751-1759) (9 p.). Cited By (since 1996): 1.
van Gestel, R.A., Rijken, P.J., Surinova, S., O'Flaherty, M., Heck, A.J.R., Killian, J.A., de Kroon, A.I.P.M. & Slijper, M. (2010). The influence of the acyl chain composition of cardiolipin on the stability of mitochondrial complexes; An unexpected effect of cardiolipin in Ī±-ketoglutarate dehydrogenase and prohibitin complexes. Journal of Proteomics, 73 (4), (pp. 806-814) (9 p.). Cited By (since 1996): 1 Eighth International Conference on Teaching Statistics.
  2009 - Wetenschappelijke publicaties
Rijken, P.J., Houtkooper, R.H., Akbari, H., Brouwers, J.F.H.M., Koorengevel, M.C., de Kruijff, B., Frentzen, M., Vaz, F.M. & de Kroon, A.I.P.M. (2009). Cardiolipin molecular species with shorter acyl chains accumulate in Saccharomyces cerevisiae mutants lacking the acyl coenzyme A-binding protein Acb1p: New insights into acyl chain remodeling of cardiolipin. Journal of Biological Chemistry, 284 (40), (pp. 27609-27619) (11 p.).
Hamelers, I.H.L., Staffhorst, R.W.H.M., Voortman, J., de Kruijff, B., Reedijk, J., van Bergen en Henegouwen, P.M.P. & de Kroon, A.I.P.M. (2009). High cytotoxicity of cisplatin nanocapsules in ovarian carcinoma celles depends on uptake by caveolae-mediated endocytosis. Clinical Cancer Research, 15, (pp. 1259-1268) (10 p.).
Hamelers, I.H.L., Staffhorst, R.W.H.M., Voortman, J., de Kruijff, B., Reedijk, J., van Bergen en Henegouwen, P.M.P. & de Kroon, A.I.P.M. (2009). High cytotoxicity of cisplatin nanocapsules in ovarian carcinoma cells depends on uptake by caveolae-mediated endocytosis. Clinical Cancer Research, 15 (4), (pp. 1259-1268) (10 p.).
Bryde, S. & de Kroon, A.I.P.M. (2009). Nanocapsules of platinum anticancer drugs: development towards therapeutic use. Future medicinal chemistry, 8 (1), (pp. 1467-1480) (14 p.).
Hamelers, I.H.L. & de Kroon, A.I.P.M. (2009). Nanocapsules of platinum-based anticancer drugs. In Andrea Bonetti (Eds.), Platinum and other heavy metal compounds in cancer chemotherapy (pp. 27-32) (383 p.). Totowa, N.J.: Humana Press.
Gubbens, J., Ruijter, E., Fays, L.E.V., Damen, J.M.A., de Kruijff, B., Slijper, M., Rijkers, D.T.S., Liskamp, R.M.J. & de Kroon, A.I.P.M. (2009). Photocrosslinking and click chemistry enable the specific detection of proteins interacting with phospholipids at the membrane interface. Chemistry and Biology, 16, (pp. 3-14) (12 p.).
Heger, M., Salles, I.I., de Kroon, A.I.P.M. & Deckmyn, H. (2009). Platelets and PEGylated lecithin liposomes: When stealth is allegedly picked up on the radar (and eaten). Microvascular Research, 78 (1), (pp. 1-3) (3 p.).
  2008 - Wetenschappelijke publicaties
Staffhorst, R.W.H.M., van der Born, K., Erkelens, C.A.M., Hamelers, I.H.L., Peters, G.J., Boven, E. & de Kroon, A.I.P.M. (2008). Antitumor activity and biodistribution of cisplatin nanocapsules in nude mice bearing human ovarian carcinoma xenografts. Anti-Cancer Drugs, 19 (7), (pp. 721-727) (7 p.).
Gubbens, J., Slijper, M., de Kruijff, B. & de Kroon, A.I.P.M. (2008). Protein complexes in bacterial and yeast mitochondrial membranes differ in their sensitivity towards dissociation by SDS. Biochimica et Biophysica Acta, 1784 (12), (pp. 2012-2018) (7 p.).
  2007 - Wetenschappelijke publicaties
de Kroon, A.I.P.M. (2007). Metabolism of phosphatidylcholine and its implications for lipid acyl chain composition in Saccharomyces cerevisiae. Biochimica et Biophysica Acta, 1771, (pp. 343-352) (10 p.).
Hamelers, I.H.L. & de Kroon, A.I.P.M. (2007). Nanocapsules: a novel formulation technology for platinum-based anticancer drugs. Future Lipidology, 2, (pp. 445-453) (9 p.).
Hamelers, I.H.L. & de Kroon, A.I.P.M. (2007). Nanocapsules: a novel lipid formulation platform for platinum-based anti-cancer drugs. Journal of Liposome Research, 17, (pp. 183-189) (7 p.).
Rijken, P.J., de Kruijff, B. & de Kroon, A.I.P.M. (2007). Phosphatidylcholine is essential for efficient functioning of the mitochondrial glycerol-3-phosphate dehydrogenase Gut2 in Saccharomyces cerevisiae. Molecular Membrane Biology, 24, (pp. 269-281) (13 p.).
Gubbens, J, Vader, P., Damen, J.M.A., O'Flaherty, M.C., Slijper, M., de Kruijff, B. & de Kroon, A.I.P.M. (2007). Probing the membrane interface-interacting proteome using photactivatable lipid cross-linkers. Journal of Proteome Research, 6, (pp. 1951-1962) (12 p.).
  2006 - Wetenschappelijke publicaties
Hamelers, I.H.L., van Loenen, E., Staffhorst, R.W.H.M., de Kruijff, B. & de Kroon, A.I.P.M. (2006). Carboplatin nanocapsules: a highly cytotoxic, phospholipid-based formulation of carboplatin. Molecular Cancer Therapeutics, 2006, (pp. 2007-2012) (6 p.).
Boumann, H.A., Gubbens, J, Koorengevel, M.C., Oh, C-S., Martin, C.E., Heck, A.J.R., Patton-Vogt, J., Henry, S.A., de Kruijff, B. & de Kroon, A.I.P.M. (2006). Depletion of phosphatidylcholine in yeast induces shortening and increased saturation of the lipid acyl chains: evidence for regulation of intrinsic membrane curvature in a eukaryote. Molecular Biology of the Cell, 17, (pp. 1006-1017) (12 p.).
Helleman, J., Burger, K.N.J., Hamelers, I.H.L., Boersma, A.W.M., de Kroon, A.I.P.M., Stoter, G. & Nooter, K. (2006). Impaired cisplatin influx in an A2780 mutant cell line. Cancer Biology and Therapy, 5 (8), (pp. 943-949) (7 p.).
de Kroon, A.I.P.M., Stafhorst, R.W.H.M., de Kruijff, B. & Burger, K.N.J. (2006). Nanocapsules: a new vehicle for intracellular delivery of drugs. In R. Harris (Eds.), Cell Biology Protocols (pp. 250-254) (5 p.). London: Wiley.
  2005 - Wetenschappelijke publicaties
de Kroon, A.I.P.M., Staffhorst, R.W.H.M., de Kruijff, B. & Burger, K.N.J. (2005). Cisplatin nanocapsules. Methods in Enzymology, 391, (pp. 118-125) (8 p.).
Boumann, H.A. & de Kroon, A.I.P.M. (2005). The contributions of biosynthesis and acyl chain remodelling to the molecular species profile of phosphatidylcholine in yeast. Biochemical Society Transactions, 33, (pp. 1146-1149) (4 p.).
  2004 - Wetenschappelijke publicaties
Chupin, V.V., de Kroon, A.I.P.M. & de Kruijff, B. (2004). Molecular architecture of nanocapsules, bilayer enclosed solid particles of cisplatin. Journal of the American Chemical Society, 126, (pp. 13816-13821) (6 p.).
Velinova, M.J., Staffhorst, R.W.H.M., Mulder, W.J.M., Dries, A.S., Jansen, B.A.J., de Kruijff, B. & de Kroon, A.I.P.M. (2004). Preparation and stability of lipid-coated nanocapsules of cisplatin: anionic phospholipid specificity. Biochimica et Biophysica Acta - Biomembranes, 1663, (pp. 135-142) (8 p.).
Boumann, H.A., de Kruijff, B., Heck, A.J.R. & de Kroon, A.I.P.M. (2004). The selective utilization of substrates in vivo by the phosphatidylethanolamine and phosphatidylcholine biosynthetic enzymes Ept1p and Cpt1p in yeast. FEBS Letters, 569, (pp. 173-177) (5 p.).
Boumann, H.A., Chin, P.T.K., Heck, A.J.R., de Kruijff, B. & de Kroon, A.I.P.M. (2004). The yeast phospholipid N-methyltransferases catalyzing the synthesis of phosphatidylcholine preferentially convert di-C16:1 substrates both in vivo and in vitro. Journal of Biological Chemistry, 279, (pp. 40314-40319) (6 p.).
Kol, M.A., de Kroon, A.I.P.M., Killian, J.A. & de Kruijff, B. (2004). Transbilayer movement of phospholipids in biogenic membranes. Biochemistry, 43, (pp. 2673-2681) (9 p.).
Kol, M.A., Kuster, D.W.D., Boumann, H.A., de Cock, J.J.P.A., Heck, A.J.R., de Kruijff, B. & de Kroon, A.I.P.M. (2004). Uptake and remodeling of exogenous phosphatidylethanolamine in E. coli. Biochimica et Biophysica Acta, 1636, (pp. 205-212) (8 p.).
  2003 - Wetenschappelijke publicaties
Kol, M.A., van Laak, A.N.C., Rijkers, D.T.S., Killian, J.A., de Kroon, A.I.P.M. & de Kruijff, B. (2003). Phospholipid flop induced by transmembrane peptides in model membranes is modulated by lipid composition. Biochemistry, 42, (pp. 231-237) (7 p.).
Boumann, H.A., Damen, J.M.A., Versluis, C., Heck, A.J.R., de Kruijff, B. & de Kroon, A.I.P.M. (2003). The two biosynthetic routes leading to posphatidylcholine in yeast produce different sets of molecular species. Evidence for lipid remodeling. Biochemistry, 42, (pp. 3054-3059) (6 p.).
  2002 - Wetenschappelijke publicaties
Janssen, M.J.F.W., de Jong, H.M., de Kruijff, B. & de Kroon, A.I.P.M. (2002). Cooperative activity of phospholipid-N-methyltransferases localized in different membranes. FEBS Letters, 513, (pp. 197-202) (6 p.).
  2001 - Wetenschappelijke publicaties
de Kroon, A.I.P.M., Ahting, U., Niggemeyer, B., Neupert, W., de Kruijff, B., Lill, R. & Diekert, K. (2001). Apocytochrome c requires the TOM complex for translocation across the mitochondrial outer membrane. EMBO Journal, 20, (pp. 5626-5635) (10 p.).
Diekert, K., de Kroon, A.I.P.M., Kispal, G. & Lill, R. (2001). Isolation and subfractionation of mitochondria from the yeast saccharomyces cerevisiae. Methods in Cell Biology, 20, (pp. 5626-5635) (10 p.).
Kol, M.A., de Kroon, A.I.P.M., Rijkers, D.T.S., Killian, J.A. & de Kruijff, B. (2001). Membrane-spanning peptides induce phospholipid flop: a model for phospholipid translocation across the inner membrane of E. coli. Biochemistry, 40, (pp. 10500-10506) (7 p.).
^ naar boven
Gegenereerd op 2018-09-22 13:15:38

Penningmeester Nederlandse Vereniging voor Biochemie en Moleculaire Biologie (NVBMB) sinds 2016

Gegenereerd op 2018-09-22 13:15:38
Volledige naam
dr. A.I.P.M. de Kroon Contactgegevens
Hugo R. Kruytgebouw

Padualaan 8
Kamer Z 806

Telefoonnummer direct 030 253 3424
Telefoonnummer afdeling 030 253 3966
Gegenereerd op 2018-09-22 13:15:38
Laatst bijgewerkt op 04-10-2016