Cutting–edge techniques and expertise in the molecular and morphological analysis.

Mission

The facilitation of advanced, qualified and standardised molecular and pathological analysis of genetically engineered animals for genetic research of academic and industrial parties.

Strategy

To provide cutting–edge techniques and expertise in the molecular and morphological analysis of genetically modified rodents and zebrafish by a team of board-certified veterinary pathologists with graduate and postgraduate training in genetics as well as cellular and molecular biology.

To offer a combined comparative molecular pathology and biomedical research training for veterinarians to earn a PhD and board certification, in order to fill the nationwide gap of veterinary pathologists1 needed for qualified analysis of genetically modified animals.

Comparative Pathological Phenotyping and Consultation

We offer full necropsy service including gross examination of tissues, slide preparation, and histological evaluation performed by board-certified veterinary pathologists specialized in analyzing rodent and zebrafish models. Pathological findings will be described, digitalized and quantified for manuscript preparation or grant applications. In addition, images will be used to develop a comprehensive database as a resource for trend analyses by bioinformatics.

Moreover, the pathological findings will be interpreted by a comparative expert team composed of veterinary and human pathologists as well as animal model scientists to evaluate the significance of the pathological findings as animal model for human diseases. Finally in vivo and in vitro studies will be suggested to further explore the molecular mechanism of the gene of interest.

Molecular Phenotyping

We offer transgenic reporter marker analysis, such as LacZ and GFP for monitoring tissue cell-type specific expression or for isolating genetically modified cells for further molecular studies. In addition we offer quantitative measurements for proliferation/ apoptosis/ differentiation/ inflammation/fibrosis status utilizing immunostainings on rodent and zebrafish tissues. Moreover we perform morphometric marker analysis as well as electronmicroscopy services.

Facilities

Facilities include necropsy rooms, tissue processing/histology laboratory, electron microscopy laboratory, immunostaining laboratory, microscopy facilities including a 5-headed microscope, digital gross and microscopic photography facilities, histomorphometry equipment including Qwin software. Equipment for evaluating blood samples is available in the University Veterinary Diagnostic Laboratory of the Companion Animal Clinic.