Time resolved fluorescence anisotropy imaging

Nanoscale interactions between proteins and/or lipids in cells can be imaged using Förster resonance energy ransfer (FRET). To study homo-clustering of such molecules, imaging methods based on homo-FRET (FRET between identical fluorophores) can be used. In this project, we developed a novel confocal time-resolved fluorescence anisotropy imaging microscope (trFAIM) that allows homo-FRET imaging. The anisotropy images can be directly converted into cluster size images that reveal the degree of clustering of the labeled protein (or lipid) in cells.