Techniques used

In our group we use an array of molecular-, genetic-, bioinformatic- and biochemistry tools to understand plant functioning and response to environmental signalling. Techniques include, but are not limited to:

  • General recombinant DNA technology. (assembly of DNA constructs, PCR, automated DNA sequence analysis; use of DNA analysis software; internet-based DNA databases analysis etc.).
  • Genetic experiments with plants: crossings, genomic DNA isolations, Southern blotting, and molecular marker analysis such as CAPS, SNP and SSLP.
  • Heterologous expression of genes in E.coli, Saccharomyces and Pichia and Yeast two-hybrid screens.
  • Transient expression assays in plant protoplasts.
  • Genetic modification of plants -mostly Arabidopsis- using the Agrobacterium tumefaciens mediated floral dip method. Selection of transgenic plantlines.
  • Analysis of gene expression in wildtype, mutant and transgenic plants: Quantitative PCR (Q-PCR), and Western blotting techniques
  • Genome wide expression profiling using Next Generation sequencing and DNA micro-arrays. (Web based) Data processing and bioinformatics. 
  • Physiological and biochemical analysis, e.g. sugar metabolism, responses to phytohormones and light. Automated advanced HPLC techniques. Protein isolations binding- and activity assays.
  • Metabolomics
  • Advanced cytogenetics techniques to visualise  chromatin and quantify compaction states, including Fluorescent in situ hybridisation (FISH) and immunolabeling.
  • Advanced microscopy, a.o. fluorescence microscopy and confocal microscopy.
  • Chromatin Immunoprecipitation (ChIP) and ChiP-sequencing techniques to identify DNA binding sites of proteins
  • Chemical genetics. Library screening, compound confirmation and optimization, application