Publications
MBB Publications 2024
van Aalst EJ, Yekefallah M, van Beekveld RAM, Breukink E, Weingarth M, Wylie BJ
Coordination of bilayer properties by an inward-rectifier K+ channel is a cooperative process driven by protein-lipid interaction
Journal of Structural Biology (2024) X9, 100101. DOI: 10.1016/j.yjsbx.2024.100101
Akdag M, van Schijndel V, Sinnige T
Islet amyloid polypeptide tagged with green fluorescent protein localises to mitochondria and forms filamentous aggregates in Caenorhabditis elegans
Biophysical Chemistry (2024) 307, 107180. DOI: 10.1016/j.bpc.2024.107180
Jekhmane S, Derks MGN, Maity S, Slingerland CJ, Tehrani KHME, Medeiros-Silva J, Charitou V, Ammerlaan D, Fetz C, Consoli NA, Cochrane RVK, Matheson EJ, van der Weijde M, Elenbaas BOW, Lavore F, Cox R, Lorent JH, Baldus M, Künzler M, Lelli M, Cochrane SA, Martin NI, Roos WH, Breukink E & Weingarth M
Host defence peptide plectasin targets bacterial cell wall precursor lipid II by a calcium-sensitive supramolecular mechanism
Nature Microbiology (2024) 9, 1778. DOI: 10.5281/zenodo.7985263
Kumar R, Breukink E, Weingarth M
Isolation and Molecular Characterization of the LTA precursor molecule Glc2-DAG, a potential target for antibiotics
ChemBioChem (2024) 25, e202400543. DOI: doi.org/10.1002/cbic.202400543
O’Brien BM, Moulick R, Jiménez-Avalos G, Rajasekaran N, Kaiser CM, Woodson SA
Stick-slip unfolding favors self-association of expanded HTT mRNA
Nature Communications (2024) 15, 8738. DOI: 10.1038/s41467-024-52764-x
Rajasekaran N, Kaiser CM
Navigating the complexities of multi-domain protein folding
Curr Opin Struct Biol (2024) 86, 102790. DOI: 10.1016/j.sbi.2024.102790
Xie M, Koch EHW, van Walree CA, Sobota A, Sonnen AFP, Killian JA, Breukink E, Lorent JH
Synergistic effects of oxidative and acid stress on bacterial membranes of Escherichia coli and Staphylococcus simulans
Commun Biol (2024) 7, 1161. DOI: 10.1038/s42003-024-06862-7
Xu Y, Wang X, Zaal EA , Berkers CR, Lorent JH, Heise T, Cox R, Pieters RJ, Breukink E
Specific labeling of newly synthesized lipopolysaccharide via metabolic incorporation of azido-galactose
BBA (2024) 1869, 4, 11, 159467. DOI:10.1016/j.bbalip.2024.159467
Yekefallah M, van Aalst EJ, van Beekveld RAM, Eason IR, Breukink E, Weingarth M, Wylie BJ
Cooperative Gating of a K+ Channel by Unmodified Biological Anionic Lipids Viewed by Solid-State NMR Spectroscopy
J Am Chem Soc (2024) 146, 4421. DOI: 10.1021/jacs.3c09266
MBB Publications 2023
Elenbaas BOW, Kremsreiter SM, Khemtemourian L, Killian JA, Sinnige T
Fibril elongation by human islet amyloid polypeptide is the main event linking aggregation to membrane damage
BBA Advances (2023) 100083. DOI: 10.1016/j.bbadva.2023.100083
Parmar A, Lakshminarayanan R, Iyer A, Goh, ETL, To TY, Yam JKH, Yang L, Newire E, Robertson MC, Prior SH, Breukink E, Madder A & Singh I
Development of teixobactin analogues containing hydrophobic, non-proteogenic amino acids that are highly potent against multidrug-resistant bacteria and biofilms
Eur. J. Med. Chem. (2023) 261, 12, 115853. DOI: 10.1016/j.ejmech.2023.115853
Shukla R, Peoples AJ, Ludwig KC, Maity S, Derks MGN, De Benedetti S, Krueger AM, Vermeulen BJA, Harbig T, Lavore F, Kumar R, Honorato RV, Grein F, Nieselt K, Liu Y, Bonvin AMJJ, Baldus M, Kubitscheck U, Breukink E, Achorn C, Nitti A, Schwalen CJ, Spoering AL, Ling LL, Hughes D, Lelli M, Roos WH, Lewis K, Schneider T & Weingarth M
An antibiotic from an uncultured bacterium binds to an immutable target
Cell (2023) 186, 19, 4059. DOI: 10.1016/j.cell.2023.07.038
Shukla R, Peoples AJ, Ludwig KC, Maity S, Derks MGN, de Benedetti S, Krueger AM, Vermeulen BJA, Lavore F, Honorato RV, Grein F, Bonvin A, Kubitscheck U, Breukink E, Achorn C, Nitti A, Schwalen CJ, Spoering AL, Ling LL, Hughes D, Lelli M, Roos WH, Lewis K, Schneider T & Weingarth M
A new antibiotic from an uncultured bacterium binds to an immutable target
BioRxiv (2023), DOI: 10.1101/2023.05.15.540765
Wang X, van Beekveld RAM, Xu Y, Parmar A, Das S, Singh I & Breukink E
Analyzing mechanisms of action of antimicrobial peptides on bacterial membranes requires multiple complimentary assays and different bacterial strains
BBA Mem. (2023) 1865, 6, 184160. DOI: 10.1016/j.bbamem.2023.184160
Xie M, Koch EHW, van Walree CA, Sobota A, Sonnen AFP, Breukink E, Killian JA & Lorent JH
Two separate mechanisms are involved in membrane permeabilization during lipid oxidation
Biophysical J. (2023), E-pub ahead of print. DOI: 10.1016/j.bpj.2023.10.028
MBB Publications 2022
Beekveld R van, Derks M, Kumar R, Smid L, Maass T, Medeiros-Silva J, Breukink EJ, Weingarth M
Specific Lipid Studies in Complex Membranes by Solid-State NMR Spectroscopy. Chem. Eur. J. (2022) e202202472. DOI:10.1002/chem.202202472
Elenbaas BOW, Khemtemourian L, Killian JA, Sinnige T
Membrane-Catalyzed Aggregation of Islet Amyloid Polypeptide Is Dominated by Secondary Nucleation. Biochemistry (2022) Jul 19;61(14):1465. DOI: 10.1021/acs.biochem.2c00184
Kopf AH, Lijding O, Elenbaas BOW, Koorengevel MC, Dobruchowska JM, van Walree CA, Killian JA
Synthesis and Evaluation of a Library of Alternating Amphipathic Copolymers to Solubilize and Study Membrane Proteins. Biomacromolecules (2022) Mar 14;23(3):743. DOI: 10.1021/acs.biomac.1c01166
Li Y, Boes A, Cui Y, Zhao S, Liao Q, Gong H, Breukink E, Lutkenhaus J, Terrak M, Du S
Identification of the potential active site of the septal peptidoglycan polymerase FtsW. PLoS Genet. (2022) 18:e1009993. DOI:10.1371/journal.pgen.1009993
Renne MF, Bao X, Hokken MWJ, Bierhuizen AS, Hermansson M, Sprenger RR, Ewing TA, Ma X, Cox RC, Brouwers JF, de Smet CH, Ejsing CS, de Kroon AIPM
Molecular species selectivity of lipid transport creates a mitochondrial sink for di-unsaturated phospholipids. EMBO J (2022) 41:e106837. DOI: 10.15252/embj.2020106837
Shukla R, Lavore F, Maity S, Derks MGN, Jones CR, Vermeulen BJA, Melcrová A, Morris MA, Becker LM, Wang X, Kumar R, Medeiros-Silva J, van Beekveld RAM, Bonvin AMJJ, Lorent JH, Lelli M, Nowick JS, MacGillavry HD, Peoples AJ, Spoering AL, Ling LL, Hughes DE, Roos WH, Breukink EJ, Lewis K & Weingarth M
Teixobactin kills bacteria by a two-pronged attack on the cell envelope. Nature (2022) 608. DOI: 10.1038/s41586-022-05019-y
Sinnige T.
Molecular mechanisms of amyloid formation in living systems. Chem Sci. (2022) May 17;13(24):7080-7097. DOI: 10.1039/d2sc01278b
Walree CA van
Intramolecular Hydrogen Bonding in DIBMA Model Compounds. Macromol. Theory Simul. (2022) 2200042. DOI: 10.1002/mats.202200042
Xu Y, Hernández-Rocamora VM, Lorent JH, Cox R, Wang X, Bao X, Stel M, Vos G, van den Bos RM, Pieters RJ, Gray J, Vollmer W, Breukink EJ
Metabolic labeling of the bacterial peptidoglycan by functionalized glucosamine. iScience (2022) 25, 104753. DOI: 10.1016/j.isci.2022.104753
MBB Publications 2021
Bao X, Koorengevel MC, Groot Koerkamp MJA, Homavar A, Weijn A, Crielaard S, Renne MF, Lorent JH, Geerts WJC, Surma MA, Mari M, Holstege FCP, Klose C, de Kroon AIPM
Shortening of membrane lipid acyl chains compensates for phosphatidylcholine deficiency in choline-auxotroph yeast. EMBO J (2021); 40:e107966. DOI: 10.15252/embj.2021107966
van Eijk M, van Dijk A, van der Ent CK, Arets HGM, Breukink E, van Os N, Adrichem R, van der Water S, Gomez RL, Kristensen M, Hessing M, Jekhmane S, Weingarth M, Veldhuizen RAW, Veldhuizen EJA, Haagsman HP
PepBiotics, novel cathelicidin-inspired antimicrobials to fight pulmonary bacterial infections. Biochimica et Biophysica Acta (2021) 1865, 9, 129951. DOI: 10.1016/j.bbagen.2021.129951
Hernández-Rocamora VM, Baranova N, Peters K, Breukink E, Loose M, Vollmer W
Real-time monitoring of peptidoglycan synthesis by membrane-reconstituted penicillin-binding proteins. eLife (2021) 10, 79, e61525. DOI: 10.7554/eLife.61525
Janson K, Zierath J, Kyrilis FL, Semchonoka DA, Hamdi F, Skalidis I, Kopf AH, Das M, Kolar C, Rasche M, Vargas C, Keller S, Kastritis PL, Meister A
Solubilization of artificial mitochondrial membranes by amphiphilic copolymers of different charge. BBA mem (2021) 12:1863. DOI: 10.1016/j.bbamem.2021.183725
Molenkamp J, Den Outer A, Van Schijndel V, Sinnige T
Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans. J. Vis. Exp. (2021) e63365. DOI:10.3791/63365
Olerinyova A, Sonn-Segev A, Gault J, Eichmann C, Schimpf J, Kopf AH, Rudden LSP, Ashkinadze D, Bomba R, Frey L, Greenwald J, Degiacomi MT, Steinhilper R, Killian JA, Friedrich T, Riek R, Struwe WB, Kukura P
Mass Photometry of Membrane Proteins. Chem. (2021) 14;7(1):224. DOI: 10.1016/j.chempr.2020.11.011. PMID: 33511302
Sassine J, Pazos M, Breukink E, Vollmer W
Lytic transglycosylase MltG cleaves in nascent peptidoglycan and produces short glycan strands. The Cell Surface (2021) 7, 100053. DOI: 10.1016/j.tcsw.2021.100053
Zhao X, Wang X, Shukla R, Kumar R, Weingarth M, Breukink E, Kuipers OP
Brevibacillin 2V Exerts Its Bactericidal Activity via Binding to Lipid II and Permeabilizing Cellular Membranes. Frontiers in Microbiology (2021) 12, 694847. DOI: 10.3389/fmicb.2021.694847
Zhao X, Wang X, Shukla R, Kumar R, Weingarth M, Breukink E, Kuipers OP
Brevibacillin 2V, a Novel Antimicrobial Lipopeptide With an Exceptionally Low Hemolytic Activity. Frontiers in Microbiology (2021) 12, 693725. DOI: 10.3389/fmicb.2021.693725
MBB Publications 2020
Castello-Serrano I, Lorent JH, Ippolito R, Levental KR, Levental I.
Myelin-Associated MAL and PLP Are Unusual among Multipass Transmembrane Proteins in Preferring Ordered Membrane Domains. J. Phys. Chem. B. 2020; 124,28:5930–5939. DOI:10.1021/acs.jpcb.0c03028.
Cunningham RD, Kopf AH, Elenbaas BOW, Staal BBP, Pfukwa R, Killian JA, Klumperman B.
Iterative RAFT-Mediated Copolymerization of Styrene and Maleic Anhydride toward Sequence- and Length-Controlled Copolymers and Their Applications for Solubilizing Lipid Membranes. Biomacromolecules. 2020; 10;21(8):3287-3300. PMID: 32672942. DOI:10.1021/acs.biomac.0c00736.
Kopf AH, Dörr JM, Koorengevel MC, Antoniciello F, Jahn H, Killian JA.
Factors influencing the solubilization of membrane proteins from Escherichia coli membranes by styrene-maleic acid copolymers. Biochim Biophys Acta Biomembr. 2020; 1862(2):183125. DOI:10.1016/j.bbamem.2019.183125.
Li M, van Raath MI, Khakpour S, Seçilir A, Sliggers BC, Huang X, Ding B, Stor, G, van der Hulst RR, de Kroon AIPM, Heger M.
In vivo assessment of thermosensitive liposomes for the treatment of port wine stains by antifibrinolytic site-specific pharmaco-laser therapy. Pharmaceutics. 2020; 12:591. DOI:10.3390/pharmaceutics12060591.
Lorent JH, Levental KR, Ganesan L, Rivera-Longsworth G, Sezgin E, Doktorova M, Lyman E, Levental I.
Plasma membranes are asymmetric in lipid unsaturation, packing and protein shape. Nature Chem Biol. 2020; 16:644–652. DOI:10.1038/s41589-020-0529-6.
Luteijn RD, Praest P, Frank Thiele F, Sadasivam SM, Singethan K, Drijfhout JW, Bach C, de Boer SM, Lebbink RJ, Tao S, Helfer M, Bach NC, Protzer U, Costa AI, Killian JA, Drexler I, Wiertz EJHJ.
A broad-spectrum antiviral peptide blocks infection of viruses by binding to phosphatidylserine in the viral envelope. Cells 2020; 9:1989. PMID: 32872420. DOI:10.3390/cells9091989.
Patton-Vogt J, de Kroon AIPM.
Phospholipid turnover and acyl chain remodeling in the yeast ER. Biochim Biophys Acta Mol Cell Biol Lipids. 2020; 1865(1). pii: S1388-1981(19)30076-9. DOI:10.1016/j.bbalip.2019.05.006.
Pawlik G, Renne MF, Kol MA, de Kroon AIPM.
The topology of the ER-resident phospholipid methyltransferase Opi3 of Saccharomyces cerevisiae is consistent with in trans catalysis. J. Biol. Chem. 2020; 295:2473-2482. DOI:10.1074/jbc.RA119.011102.
Renne MF, Bao X, de Kroon AIPM.
Water soluble lipid precursor contaminants in yeast culture medium ingredients. FEMS Yeast Res. 2020; 20(5). DOI:10.1093/femsyr/foaa029.
Shukla R, Medeiros-Silva J, Parmar A, Vermeulen BJA, Das S, Lucini Paioni A, Jekhmane S, Lorent JH, Bonvin AMJJ, Baldus M, Lelli M, Veldhuizen EJA, Breukink E, Singh I, Weingarth M.
Mode of action of teixobactins in cellular membranes. Nature Commun. 2020; 11:2848. DOI:10.1038/s41467-020-16600-2.
Yurtsever D, Lorent JH
Structural Modifications Controlling Membrane Raft Partitioning and Curvature in Human and Viral Proteins. J Phys Chem B. 2020 Sep 3; 124(35):7574–7585. PMID: 32813532. DOI:10.1021/acs.jpcb.0c03435.
MBB Publications 2019
Anaokar S, Kodali R, Jonik B, Renne MF, Brouwers JFHM, Lager I, de Kroon AIPM, Patton-Vogt J.
The glycerophosphocholine acyltransferase Gpc1 is part of a phosphatidylcholine (PC)-remodeling pathway that alters PC species in yeast. J Biol Chem. 2019 Jan 25;294(4):1189-1201. doi: 10.1074/jbc.RA118.005232. Epub 2018 Dec 4.
Boes A, Olatunji S, Breukink E, Terrak M.
Regulation of the Peptidoglycan Polymerase Activity of PBP1b by Antagonist Actions of the Core Divisome Proteins FtsBLQ and FtsN. MBio. 2019 Jan 8;10(1). pii: e01912-18. doi: 10.1128/mBio.01912-18
Domínguez Pardo JJ, van Walree CA, Egmond MR, Koorengevel MC, Killian JA.
Nanodiscs bounded by styrene-maleic acid allow trans-cis isomerization of enclosed photoswitches of azobenzene labeled lipids Chem Phys Lipids. 2019 Feb 16. pii: S0009-3084(18)30245-7. doi: 10.1016/j.chemphyslip.2019.02.002. [Epub ahead of print
Kopf AH, Koorengevel MC, van Walree CA, Dafforn TR, Killian JA.
A simple and convenient method for the hydrolysis of styrene-maleic anhydride copolymers to styrene-maleic acid copolymers. Chem Phys Lipids. 2019 Jan;218:85-90. doi: 10.1016/j.chemphyslip.2018.11.011. Epub 2018 Dec 1
Kuru E, Radkov A, Meng X, Egan A, Alvarez L, Dowson A, Booher G, Breukink E, Roper DI, Cava F, Vollmer W, Brun Y, VanNieuwenhze MS.
Mechanisms of Incorporation for D-Amino Acid Probes That Target Peptidoglycan Biosynthesis. ACS Chem Biol. 2019 Dec 20;14(12):2745-2756. doi: 10.1021/acschembio.9b00664. Epub 2019 Dec 5.
Medeiros-Silva J, Jekhmane S, Breukink E, Weingarth M.
Towards the native binding modes of Lipid II targeting antibiotics. Chembiochem. 2019 Feb 6. doi: 10.1002/cbic.201800796. [Epub ahead of print]
Morè N, Martorana AM, Biboy J, Otten C, Winkle M, Serrano CKG, Montón Silva A, Atkinson L, Yau H, Breukink E, den Blaauwen T, Vollmer W, Polissi A.
Peptidoglycan Remodeling Enables Escherichia coli To Survive Severe Outer Membrane Assembly Defect. MBio. 2019 Feb 5;10(1). pii: e02729-18. doi: 10.1128/mBio.02729-18.
Mueller EA, Egan AJ, Breukink E, Vollmer W, Levin PA.
Plasticity of Escherichia coli cell wall metabolism promotes fitness and antibiotic resistance across environmental conditions. Elife. 2019 Apr 9;8. pii: e40754. doi: 10.7554/eLife.40754
Pazos M, Peters K, Casanova M, Palacios P, VanNieuwenhze M, Breukink E, Vicente M, Vollmer W.
Publisher Correction: Z-ring membrane anchors associate with cell wall synthases to initiate bacterial cell division. Nat Commun. 2019 Jan 30;10(1):591. doi: 10.1038/s41467-019-08410-y.
Saravanan MS, Ryazanov S, Leonov A, Nicolai J, Praest P, Giese A, Winter R, Khemtemourian L, Griesinger C, Killian JA.
The small molecule inhibitor anle145c thermodynamically traps human islet amyloid peptide in the form of non-cytotoxic oligomers. Sci Rep. 2019 Dec 13;9(1):19023. doi: 10.1038/s41598-019-54919-z.
Schildknegt D, Lodder N, Pandey A, Egmond MR, Pena F, Braakman I, van der Sluijs P.
Characterization of CNPY5 and its family members. Protein Sci. 2019 Jul;28(7):1276-1289. doi: 10.1002/pro.3635. Epub 2019 May 16
van Willigen M, Vonk AM, Yeoh HY, Kruisselbrink E, Kleizen B, van der Ent CK, Egmond MR, de Jonge HR, Braakman I, Beekman JM, van der Sluijs P.
Folding-function relationship of the most common cystic fibrosis-causing CFTR conductance mutants. Life Sci Alliance. 2019 Jan 18;2(1). pii: e201800172. doi: 10.26508/lsa.201800172. Print 2019 Feb.
MBB Publications 2018
Dominguez Pardo JJ, Koorengevel MC, Uwugiaren N, Weijers J, Kopf AH, Jahn H, van Walree CA, van Steenbergen MJ, Killian JA
Membrane Solubilization by Styrene-Maleic Acid Copolymers: Delineating the Role of Polymer Length. Biophys J. 2018 Jul3; 115(1):129-138 doi 10.1016/bpj 2018.05.032
Egan AJF, Maya-Martinez R, Ayala I, Bougault CM, Banzhaf M, Breukink E, Vollmer W, Simorre JP
Induced conformational changes activate the peptidoglycan synthase PBP1B. Mol. Microbiol. 2018; 110:335. doi: 10.1111/mmi.14082
Henrich E, Löhr F, Pawlik G, Peetz O, Dötsch V, Morgner N, de Kroon AIPM, Bernhard F
Lipid Conversion by Cell-Free Synthesized Phospholipid Methyltransferase Opi3 in Defined Nanodisc Membranes Supports an in Trans Mechanism. Biochemistry 2018; 57:40. doi: 10.1021/acs.biochem.8b00807
Hernández-Rocamora VM, Otten CF, Radkov A, Simorre JP, Breukink E, VanNieuwenhze M, Vollmer W.
Coupling of polymerase and carrier lipid phosphatase prevents product inhibition in peptidoglycan synthesis. Cell Surf. 2018; 2:1. doi: 10.1016/j.tcsw.2018.04.002
Hoffmann ARF, Saravanan MS, Lequin O, Killian JA, Khemtemourian L
A single mutation on the human amyloid polypeptide modulates fibril growth and affects the mechanism of amyloid-imduced membrane damage. Biochim Biophys Acta 2018 Feb 27 doi 10.1016/j.bbamem.2018.02.018
Jiang H, Tang X, Zhou Q, Zou J, Li P, Breukink E, Gu Q
Plantaricin NCB from Lactobacillus plantarum causes cell membrane disruption to Micrococcus luteus without targeting lipid II. Apl. Microbiol Biotechnol. 2018 Jul 7 doi: 10.1007.s00253-018-9182-3
Liu Y, Moura ECCM, Dörr JM, Scheidelaar S, Heger M, Egmond MR, Killian JA, Mohammadi T, Breukink E.
Bacillus subtilis MraY in detergent-free system of nanodiscs wrapped by styrene-maleic acid copolymers.PLoS One. 2018 Nov 5;13(11):e0206692. doi: 10.1371/journal.pone.0206692. eCollection 2018
Medeiros-Silva J, Jekhmane S, Lucini Paioni A, Gawarecka K, Baldus M, Swiezewska E, Breukink E, Weingarth M
High-resolution NMR studies of antibiotics in cellular membranes Nature Communications 2018; 9:3963. doi: 10.1038/s41467-018-06314-x
Montón Silva A, Otten C, Biboy J, Breukink E, VanNieuwenhze M, Vollmer W, den Blaauwen T.
The Fluorescent D-Amino Acid NADA as a Tool to Study the Conditional Activity of Transpeptidases in Escherichia coli.Front Microbiol. 2018 Sep 4;9:2101. doi: 10.3389/fmicb.2018.02101. eCollection 2018.
Morlot C, Straume D, Peters K, Hegnar OA, Simon N, Villard AM, Contreras-Martel C, Leisico F, Breukink E, Gravier-Pelletier C, Le Corre L, Vollmer W, Pietrancosta N, Håvarstein LS, Zapun A.
Structure of the essential peptidoglycan amidotransferase MurT/GatD complex from Streptococcus pneumoniae.Nat Commun. 2018 Aug 9;9(1):3180. doi: 10.1038/s41467-018-05602-w.
Pazos M, Peters K, Casanova M, Palacios P, VanNieuwenhze M, Breukink E, Vicente M, Vollmer W
Z-ring membrane anchors associate with cell wall synthases to initiate bacterial cell division. Nat Commun. 2018 Nov 30;9(1):5090. doi: 10.1038/s41467-018-07559-2.
Renne MF, de Kroon AIPM
The role of phospholipid molecular species in determining the physical properties of yeast membranes. FEBS Letters 2018; 592:13300-1345. doi: 10.1002/1873-3468.12944. PMID: 5947837
MBB Publications 2017
Bersch B, Dörr JM, Hessel A, Killian JA, Schanda P
Proton-Detected Solid-State NMR Spectroscopy of a Zinc Diffusion Facilitator Protein in Native Nanodiscs. Angew Chem Int Ed Engl. 2017 Jan 27. doi: 10.1002/anie.201610441. PMID: 28128538
Calvez P, Breukink E, Roper DI, Dib M, Contreras-Martel C, Zapun A
Substitutions in PBP2b from β-lactam resistant Streptococcus pneumoniae have different effects on enzymatic activity and drug reactivity. J Biol Chem. 2017 Jan 6. pii: jbc.M116.764696. doi: 10.1074/jbc.M116.764696. PMID: 28062575
De Kroon AIPM
Lipidomics in research on yeast membrane lipid homeostasis. Biochim Biophys Acta. 2017 Aug;1862(8):797-799. doi: 10.1016/j.bbalip.2017.02.007. Epub 2017 Feb 17. PMID: 28219720
Dominguez Pardo JJ, Dörr JM, Iyer A, Cox RC, Scheidelaar S, Koorengevel MC, Subramaniam V, Killian JA
Solubilization of lipids and lipid phases by the styrene-maleic acid copolymer. Eur Biophys J. 2017 Jan;46(1):91-101. doi: 10.1007/s00249-016-1181-7. PMID: 27815573
Dominguez Pardo JJ, Dörr JM, Renne MF, Ould-Braham T, Koorengevel MC, van Steenbergen MJ, Killian JA
Thermotropic properties of phosphatidylcholine nanodiscs bounded by styrene-maleic acid copolymers. J. Chem Phys Lip 2017 Nov;208:58-64. doi: 10.1016/j.chemphyslop.2017.08.0101
Dörr JM, van Coevorden MH, Hoogenraad CC, Killian JA
Solubilization of human cells by the styrene-maleic acid copolymer: insights from fluorescence microscopy. Biochim Biophys Acta 2017 Nov;1859(11):2155-2160. doi: 10.1016/j.bbamem.2017.08.010.
't Hart P, Wood T, Hajmohammadebrahimtehrani K, van Harten R, Sleszynska M, Breukink E, Martin N
De novo identification of lipid II binding lipopeptides with antibacterial activity against vancomycin-resistant bacteria. Chem. Sci. 2017;8:7991-7997 doi: 10.1039/c7sc03413.
Khemtemourian L, Guillemain G, Foufelle F, Killian JA
Residue specific effects of human islet polypeptide amyloid on self-assembly and on cell toxicity. Biochimie. 2017 Aug; pii: S0300-9084(17)30186-4. doi: 10.1016/j.biochi.2017.07.015. PMID: 28778718
Leclercq S, Derouaux A, Olatunji S, Fraipont C, Egan AJ, Vollmer W, Breukink E, Terrak M
Interplay between Penicillin-binding proteins and SEDS proteins promotes bacterial cell wall synthesis. Sci Rep. 2017 Feb 24;7:43306. doi: 10.1038/srep43306. PMID: 28233869
Parmar A, Prior SH, Iyer A, Vincent CS, Van Lysebetten D, Breukink E, Madder A, Taylor EJ, Singh I
Defining the molecular structure of teixobactin analogues and understanding their role in antibacterial activities. Chem Commun (Camb). 2017 Jan 26. doi: 10.1039/c6cc09490b. PMID: 28124045
Reiniers MJ, van Golen RF, Bonnet S, Broekgaarden M, van Gulik TM, Egmond MR, Heger M
Preparation and Practical Applications of 2',7'-Dichlorodihydrofluorescein in Redox Assays. Anal Chem. 2017 Apr 4;89(7):3853-3857. doi: 10.1021/acs.analchem.7b00043. Epub 2017 Mar 15. PMID: 28224799
Swainsbury DJK, Scheidelaar S, Foster N, van Grondelle R, Killian JA, Jones MR
The effectiveness of styrene-maleic acid (SMA) copolymers for solubilisation of integral membrane proteins from SMA-accessible and SMA-resistant membranes.Biochim Biophys Acta. 2017 Jul 24. pii: S0005-2736(17)30236-5. doi: 10.1016/j.bbamem.2017.07.011. PMID: 28751090. Epub 2017 Jul 24
MBB Publications 2016
Cleverley RM, Rismondo J, Lockhart-Cairns MP, Van Bentum PT, Egan AJ, Vollmer W, Halbedel S, Baldock C, Breukink E, Lewis RJ Subunit Arrangement in GpsB, a Regulator of Cell Wall Biosynthesis Microb.Drug Resist. 2016 Jun 3
Liu Y, Rodrigues JP, Bonvin AM, Zaal EA, Berkers CR, Heger M, Gawarecka K, Swiezewska E, Breukink E, Egmond MR New insight in the catalytic mechanism of bacterial MraY from enzyme kinetics and docking studies J.Biol.Chem. 2016 291(29):15057-68
Kleijn LH, Oppedijk SF, 't Hart P, van Harten RM, Martin-Visscher LA, Kemmink J, Breukink E, Martin NI Total Synthesis of Laspartomycin C and Characterization of Its Antibacterial Mechanism of Action J.Med.Chem. 2016 59(7):3569-74
Broekgaarden M, van Vught R, Oliveira S, Roovers RC, van Bergen En Henegouwen PM, Pieters RJ, Van Gulik TM, Breukink E, Heger M Site-specific conjugation of single domain antibodies to liposomes enhances photosensitizer uptake and photodynamic therapy efficacy. Nanoscale 2016 8(12):6490-4
Renne MF, Bao X, De Smet CH, de Kroon AI Lipid Acyl Chain Remodeling in Yeast Lipid Insights. 2016 Jan 19;8(Suppl 1):33-40. doi: 10.4137/LPI.S31780.
Gruber JM, Scheidelaar S, van Roon H, Dekker JP, Killian JA, van Grondelle R Photophysics in single light-harvesting complexes II: from micelle to native nanodisks Proc. of SPIE 2016 Vol. 9714 97140A-1
Dörr JM, Scheidelaar S, Koorengevel MC, Dominguez JJ, Schäfer M, van Walree CA, Killian JA The styrene-maleic acid copolymer: a versatile tool in membrane research Eur Biophys J. 2016 Jan;45(1):3-21.
Oppedijk SF, Martin NI, Breukink E Hit 'em where it hurts: The growing and structurally diverse family of peptides that target lipid-II Analogues. Biochemistry. 2016 Jan 12;55(1):232-7. doi: 10.1021/acs.biochem.5b01173.
MBB Publications 2015
‘t Hart P, Oppedijk S.F., Breukink E, Martin N.I. New Insights into Nisin’s Antibacterial Mechanism Revealed by Binding Studies with Synthetic Lipid II Analogues Biochemistry 2015 Dec 22
Sosic I, Anderluh M, Sova M, Gobec M, Mlinaric Rašcan I, Derouaux A, Ana Amoroso A, Terrak M, Breukink E, and Gobec S Structure-Activity Relationships of Novel Tryptamine-Based Inhibitors of Bacterial Transglycosylase. J Med Chem. 2015 Dec24;58(24):9712-21
Egan AJ, Biboy J, Van ‘t Veer I, Breukink E, Vollmer W Activities and regulation of peptidoglycan synthases. Philos Trans R Society Lond B Biology Science. 2015 Oct 5; 370(1679)
Olthof ED, Gulich AF, Renne MF, Landman S, Joosten LA, Roelofs HM, Wanten GJ Immune activation by medium-chain triglyceride-containing lipid emulsions is not modulated by n-3 lipids or toll-like receptor 4. Toxicol In Vitro 2015 Oct;29(7): 1851-8
Blanchaert B, Wyseure T, Breukink E, Adams E, Declerck P, Van Schepdael A Development of a liquid chromatography/mass spectrometry assay for the bacterial transglycosylation reaction through measurement of Lipid II Electrophoresis. 2015 Sep 3
Furse S, Wienk H, Boelens R, de Kroon AI, Killian JA E. coli MG1655 modulates its phospholipid composition through the cell cycle FEBS let 2015 Sep 14;589(19 PT B):2726-30
Furse S, de Kroon AI Phosphatidylcholine's functions beyond that of a membrane brick Mol Membr Biol. 2015 32(4):117-9
Furse S, Egmond MR, Killian JA Isolation of lipids from biological samples. Mol Membr Biol. 2015 32(3): 55-64
Koopmans T, Wood TM, 't Hart P, Kleijn LH, Hendrickx AP, Willems RJ, Breukink E, Martin NI Semisynthetic Lipopeptides Derived from Nisin Display Antibacterial Activity and Lipid II Binding on Par with That of the Parent Compound. J Am Chem Soc. 2015 Jul 29;137(29):9382-9. doi: 10.1021/jacs.5b04501.
Slootweg JC, van Herwerden EF, van Doremalen MF, Breukink E, Liskamp RM, Rijkers DT Synthesis of nisin AB dicarba analogs using ring-closing metathesis: influence of sp(3)versus sp(2) hybridization of the a-carbon atom of residues dehydrobutyrine-2 and dehydroalanine-5 on the lipid II binding affinity. Org Biomol Chem. 2015 May 19;13(21):5997-6009
Gray AN, Egan AJ, Van't Veer IL, Verheul J, Colavin A, Koumoutsi A, Biboy J, Altelaar AF, Damen MJ, Huang KC, Simorre JP, Breukink E, den Blaauwen T, Typas A, Gross CA, Vollmer W Coordination of peptidoglycan synthesis and outer membrane constriction during Escherichia coli cell division. Elife 2015 May7;4
Cybulski LE, Ballering J, Moussatova A, Inda ME, Vazquez DB, Wassenaar TA, de Mendoza D, Tieleman DP, Killian JA Activation of the bacterial thermosensor DesK involves a serine zipper dimerization motif that is modulated by bilayer thickness.Proc Natl Acad Sci U S A. 2015 ; 112(20):6353-8
Spelbrink RE, Lensing H, Egmond MR, Giuseppin ML Potato Patatin Generates Short-Chain Fatty Acids from Milk Fat that Contribute to Flavour Development in Cheese Ripening Appl Biochem Biotechnol. 2015 Mar 27.
Scheidelaar S, Koorengevel MC, Pardo JD, Meeldijk JD, Breukink E, Killian JA Molecular model for the solubilization of membranes into nanodisks by styrene maleic Acid copolymers. Biophys J. 2015 Jan 20;108(2):279-90
Bury D, Dahmane I, Derouaux A, Dumbre S, Herdewijn P, Matagne A, Breukink E, Mueller-Seitz E, Petz M, Terrak M Positive cooperativity between acceptor and donor sites of the peptidoglycan glycosyltransferase. Biochem Pharmacol. 2015 Jan 15;93(2):141-50
MBB Publications 2014
Slootweg JC, Peters N, Quarles van Ufford HL, Breukink E, Liskamp RM, Rijkers DT (2014), Semi-synthesis of biologically active nisin hybrids composed of the native lanthionine ABC-fragment and a cross-stapled synthetic DE-fragment 1;22(19):5345-53.
Dörr JM, Koorengevel MC, Schäfer M, Prokofyev AV, Scheidelaar S, Van der Cruijsen EAW, Dafforn TR, Baldus M, Killian JA (2014), Detergent-free isolation, characterization and functional reconstitution of a tetrameric K+ channel: the power of native nanodiscs Proc Natl Acad Sci U S A. 111, 18607-18612.
David J. K. Swainsbury,+ Stefan Scheidelaar,+ Rienk van Grondelle, J. Antoinette Killian,* and Michael R. Jones* (2014), Bacterial Reaction Centers Purified with Styrene Maleic Acid Copolymer Retain Native Membrane Functional Properties and Display Enhanced Stability**. Angew Chem Int Ed Engl. 53, 11803-11807.
Luteijn RD, Hoelen H, Kruse E, van Leeuwen WF, Grootens J, Horst D, Koorengevel M, Drijfhout JW, Kremmer E, Früh K, Neefjes JJ, Killian A, Lebbink RJ, Ressing ME, Wiertz EJ (2014), Cowpox Virus Protein CPXV012 Eludes CTLs by Blocking ATP Binding to TAP. J Immunol. 193, 1578-1589.
Mohammadi T, Breukink E., The Chlamydial Anomaly Clarified? Chembiochem. 2014 Jun 2. doi: 10.1002/cbic.201402143
Egan AJ, Jean NL, Koumoutsi A, Bougault CM, Biboy J, Sassine J, Solovyova AS, Breukink E, Typas A, Vollmer W, Simorre JP., Outer-membrane lipoprotein LpoB spans the periplasm to stimulate the peptidoglycan synthase PBP1B. Proc Natl Acad Sci U S A. 2014 May 12
van Vught R, Pieters RJ, Breukink E., Site-specific functionalization of proteins and their applications to therapeutic antibodies. Comput Struct Biotechnol J. 2014 Feb 14;9:e201402001
Mohammadi T, Sijbrandi R, Lutters M, Verheul J, Martin N, den Blaauwen T, de Kruijff B, Breukink E., Specificity of the transport of Lipid II by FtsW in Escherichia coli. J Biol Chem. 2014 Apr 7