Maxillofacial Bone Regeneration

Our lab aims to develop regenerative therapies targeting bone defects. We adopt cell-based and cell-free strategies across the spectrum from basic lab studies to pre-clinical and clinical trials with a focus on regeneration of bone, cartilage and blood vessels.

We are a young group working on bone regeneration of the facial structures. Patients with maxillofacial bone defects need bone tissue substitutes to reshape their face, to speak and to anchor teeth for chewing food. These bone defects may arise from an accident, cancer, infection or due to congenital malformations. Examples of defects include cleft palate, sinus floor augmentation, alveolar augmentation or segmental mandibular defects.

Our team designs bio-inspired regenerative approaches to restore such defects. We employ stem cell technologies or biomaterials advances with state-of-the-art regenerative technologies to recapitulate natural tissue development and regeneration.

The majority of our research projects aim at two cell-based routes to bone regeneration, inspired by natural mechanisms.

1) Recapitulation of endochondral bone formation so far has proven to offer great potential for translation into clinical application. This concept is based on the implantation of an engineered (lab-grown) cartilage tissue that following implantation can be remodeled by the body into patient-own bone. A modular strategy is currently adopted where small cartilage tissues are engineered that serve as building blocks to assemble a bone template of desired size and shape. Our modules are non-living, which enables scale-up, storage and off-the-shelf use. Current efforts are under the XLbone project (NWO).

2) The creation of large engineered tissue constructs requires the introduction of vasculature. These prevascularized bone constructs could be applied in large clinical defects with limited vascular supply, including e.g. mandibular defects after oncological resections or in non-unions. We have established methods to create capillary-like networks in biomaterial-based lab cultures. Also, regeneration of small diameter blood vessels is researched in our group. Current efforts are directed at the generation of multi-scale vascular networks and in vitro models.

Smaller projects in our lab are for example exploring the use of dental pulp stem cells and calcium phosphates to restore bone defects.

Contact for internships

Dr. Debby Gawlitta: D.Gawlitta@umcutrecht.nl

People

Name

Position

Contact/Linkedin

Debby Gawlitta, PhD

Associate Professor

LinkedIn

Kenny Man, PhD

Postdoc

 

Flurina Staubli

PhD student

 

Jonelle Meijer

PhD student

 

Rui He

PhD student

 

Tianyu Yang

PhD student

 

Nada Rahmani

PhD student

 

Alumni

Name

Past position

Additional info

Leanne de Silva

PhD in 2024

Clinical Translational Aspects in Bone Regeneration - From Bench to Bone

Paree Khokhani

PhD in 2024

Immunomodulatory Pathogen Recognition Receptor Ligands for Bone Regeneration

Lucas van Dijk

PhD in 2022

Enhancing bone regeneration by calcium phosphates with surface topography -

A translational evaluation of synthetic bone graft substitutes

Iris Pennings

 

PhD in 2020

Bioengineering of pre-vascularized bone tissue analogues

NBTE Thesis award 2019-2021

Alessia Longoni

PhD in 2020

Endochondral bone regeneration: stepping stones towards clinical translation

Barbara Klotz

PhD in 2020

Engineering Gelatin-Based Biomatrices for Pre-vascularisation of Bone Analogues

Lizette Utomo

Postdoc, 2018-2020

MACRON project (mandibular condyle regeneration)

Willemijn Boot

PhD in 2018

Recent developments in diagnosis and prophylaxis of orthopaedic implant-related infections

Marianne Koolen

PhD in 2018

Building better bone

Vivian Mouser

PhD in 2017

Bio-inks for 3D printing of cartilage implants – Tailoring gelMA and polyHPMA-lac-PEG hydrogels for the fabrication of spatially organized constructs

Jetze Visser

 

PhD in 2015 (cum laude)

 

Biofabrication of implants for articular joint repair - Cartilage regeneration in reinforced gelatin-based hydrogels

NBTE Thesis award 2013-2015

Linda Kock

Postdoc, 2012-2014