Living Matter Engineering and Biofabrication

Engineering and materials science help us to understand how stem cells behave. We translate this knowledge to build lab-made 3D tissues for regenerative medicine and personalized disease models.

The function of living tissues and organs is intimately linked to their architecture. In our lab, we are fascinated by how cells interact with this complex environment, communicate with each other, sense and remodel their surroundings, to determine how tissues develop and function, both in healthy and diseased states. In our research, we investigate how to capture this dynamics in vitro to build lab-made, engineered tissues for personalized therapies, disease models and regenerative medicine. To achieve this goal, we develop advanced biofabrication and bioprinting technologies, smart cell-instructive biomaterials and state-of-the-art stem cell technologies. 

The lab comprises a multidisciplinary team of scientists, from biologists to biomedical engineers, chemists and physicists from Utrecht University and from the University Medical Center Utrecht. The team, in collaboration with the Malda lab, contributes to and manages the Utrecht Biofabrication facility.

Biofabrication technologies

Biofabrication, relying on automated bioprinting and bioassembly technologies, allow great freedom of design to precisely control the spatial positioning and patterning of multiple cell types and materials. At the LevatoLab, we are at the forefront of biofabrication technology development to enhance the complexity and functionality of our tissue engineered constructs. We focus on the design of new approaches, multi-technology printing platforms and applications of a broad array of 3D (bio)printing technologies, including Digital light projection (DLP) printing, Extrusion-based bioprinting (EBB), Melt and Cell Electrowriting (MEW), Laser induced-forward transfer (LIFT), Fused depoistion modeling (FDM). In addition of these layer-by-layer manufacturing approach, our lab pioneered the development of Volumetric Bioprinting (VBP), which we investigate for sculpting complex and large living constructs in a matter of few seconds. We actively integrate artificial intelligence and advanced automated control tools into bioprinting processes. 

See also: Bio-inks for printing living tissues (Eye-opener video with Riccardo Levato)

Biomaterials Design

In order to harness the structural and biological potential our tissue engineering approaches have to offer, the development of smart biomaterials that are stimuli-responsive and possess bioactive components is essential. We design a wide array of novel materials, scaffolds for tissue engineering, and hydrogels as printable bioinks and bioresins that interact with cells and mimic the native dynamics of the extracellular milieu, with the goal of enhancing the biological functionality of (printed) cells, and boost material-guided tissue maturation and regeneration. We work with a wide array of natural-origin materials (gelatin, silk, hyaluronan, etc.), as well as synthetic hydrogels and biodegradable polyesters, investigating novel photochemistries and dynamic and supremolecular interactions. 

Bioengineering  Functional Living Systems from Stem Cells and Organoids

With state-of-the-art organoids and stem cell, our aim is to develop tissue analogues with native-like functionality. Moreover, we investigate novel cell engineering and synthetic biology tools (i.e. optogenetics) for their potential to enhance the therapeutic capacity of (stem) cells. We study how smart cell-instructive biomaterials and advanced biofabrication technologies can be used to drive morphogenesis from cultured cells, and assess the emergence of organ-level functions that are lacking in insufficiently organized structures. Be it as implantable tissue engineered constructs, organ-on-a-chip devices, or advanced in vitro models for drug testing and disease modeling, the Levato Lab research focuses on a wide array of tissue-specific applications, most notably pancreas tissue engineering and diabetes research, bone and bone marrow modelling, vascularization in tissue engineering, liver, cardiac tissue repair, breast tissue and cancer modelling.  

Contact for internships

Dr. ir. Riccardo Levato: R.Levato@uu.nl

People

Name 

Position 

Contact/Linkedin 

Riccardo Levato 

Associate Professor 

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Paulina Nunez Bernal 

Postdoctoral Researcher 

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Alessia Longoni 

Postdoctoral Researcher 

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Oksana Dudaryeva 

Postdoctoral Researcher 

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Pere Catala Quilis 

Postdoctoral Researcher 

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Marième Gueye 

PhD-student 

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Davide Ribezzi 

PhD-student 

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Gabriel Grossbacher 

PhD-student 

 

Marc Falandt 

PhD-student 

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Estée Grandidier 

PhD-student 

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Maj-Britt Buccholz 

PhD-student 

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Nuria Gines Rodriguez 

PhD-student 

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Sammy Florczak 

PhD-student 

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Camille Bonhomme 

PhD-student 

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Gaja Zanoni 

PhD-student 

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Bram Nijhoff 

Research technician 

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Charlotte Brice 

Research technician 

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