Utrecht Monoclonal Antibody Facility
Monoclonal antibodies are important for specific basic research, diagnostics and therapeutics. The facility offers:
- Immunisation of mice, wild type or knock out
- Screening of serum titers
- Generation and screening of hybridomas
- IgG2c antibodies
New Immunisation method
We offer a new and very efficient cellular immunisation method. With this method, antibodies can be raised against low immunogenic membrane proteins, such as multispanning membrane proteins, conformational epitopes and we can generate agonistic and antagonistic mABs. On request we can make mABs against soluble proteins/peptides with Complete Freund’s adjuvant.
Procedure/Services UMAB
- Dossier management for ethical approval (Animal Experiments Committee)
- Handling of mice
- Collecting spleen of positive mice
- Fusion of spleen cells to generate hybridomas
- Selection of positive hybridomas
- Long term storage
Extra services such as antibody purification on request.
Contact
Jeanette Leusen
University Medical Center Utrecht
Laboratory for Translational Immunology
Heidelberglaan 100
3584 CX Utrecht
Email: j.h.w.leusen@umcutrecht.nl
Website: bit.ly/Utrecht-UMAB