8 October 2018

Protein complex plays essential role in maintaining bacterial membrane

Novel functional insight in protein complex, possible new target for antibiotics

Researchers at Utrecht University have gained new insights into the stucture and function of a protein complex that maintains the outer membrane of a bacteria. Shutting down this protein complex makes it impossible for the membrane to maintain itself, causing the bacteria to die. This protein could present a promising  target for novel antibiotics. The researchers will publish their findings in Nature Communications on 8 October.

“Many of our current antibiotics kill bacteria by making a hole in the outer membrane”, says Marc Baldus, Professor of NMR Spectroscopy and lead author of the publication. “With this new knowledge, we now know that we can attack the membrane in another way: by preventing the bacteria from maintaining its membrane. It’s as if we’d found another way to sabotage a car: not by destroying the engine, but by deactivating the oil pump, which causes the car to overheat and break down on its own.”

950 MHz
The NMR facility at Utrecht University

Protein complex in action

Scientists have long known that this protein complex plays a role in the bacteria’s outer membrane. “But we didn’t know exactly what role that was”, Baldus explains. The researchers studied the protein complex using NMR, nuclear magnetic resonance in functional membranes. “It works a bit like an MRI scanner at a hospital, but then at the atomic level. For the first time, we’ve been able to watch the protein complex in action, which helps us to better understand how the protein machine works, and that it is essential in keeping the cell alive.”

New antibiotics

The new insights could lead to new types of antibiotics, Baldus continues. “Bacteria that have developed a resistance to current antibiotics are still susceptible to this new area of attack.” Antibiotics resistance is a growing problem, with an increasing number of bacteria that are able to defend against the medication available at the moment. “We’ll still need to do a lot more research to develop this new type of antibiotics, but this knowledge is a vital first step forward.”

Publication

Formation of the β-barrel assembly machinery complex in lipid bilayers as seen by solid-state NMR
Cecilia Pinto, Deni Mance, Tessa Sinnige, Mark Daniëls, Markus Weingarth and Marc Baldus*
Nature Communications, 8 October 2018, DOI 10.1038/s41467-018-06466-w
*All authors are affiliated with Utrecht University.