Hematopoietic stem cells (HSCs) are responsible for the production of all mature hematopoietic cells during the entire life of an organism. It is now known that HSCs are generated from a specific subset of endothelial cells in the main arteries of the developing mouse and zebrafish embryos. In the aorta of the mouse embryo, HSCs are first detected as part of intra-aortic hematopoietic clusters, transient small aggregates of cells in contact with the blood circulation. In the zebrafish embryo, HSCs undergo a sub-aortic ingression before being released into the blood stream. In both species, HSCs are later amplified in secondary sites before migrating to the bone or kidney marrow where they will reside during adulthood. In the past ten years, technological advances such as single-cell mRNA Sequencing, have deepened our understanding of the molecular mechanisms underlying the endothelial to hematopoietic transition. Additionally, novel sophisticated lineage-tracing methods now allow us to track the progeny of single hematopoietic cells. This thesis aim is to characterize the mechanisms underlying HSC production and function in the mouse and zebrafish. In addition, this thesis contains a variety of novel methods derived from single-cell mRNA-Sequencing technologies that have been applied in the field of hematopoiesis.
17 May 2018 from 14:30 to 15:30
PhD Defence: The single-cell transcriptional landscape of hematopoiesis
Start date and time17 May 2018 14:30
End date and time17 May 2018 15:30
PhD candidateChloé Baron
DissertationThe single-cell transcriptional landscape of hematopoiesis
PhD supervisor(s)Alexander van Oudenaarden