PhD defence: Synthesis and Functional Characterization of Novel Chimeric Vaccine Adjuvants & Chemo-Enzymatic Approaches to Synthesize Heparan Sulfate Oligosaccharides

to

The work described in this thesis focuses on the development of novel chimeric vaccine adjuvants and the development of novel controlled chemo-enzymatic methods to synthesize a diverse library of heparan sulfate oligosaccharides for structure-activity study.

In chapter 1, a general introduction is given on vaccines and vaccine adjuvants. Vaccination is the most important medical achievement of mankind. It protects human from various destructive diseases, improves the quality of life, and extends the average life span.

Chapter 2 introduces the biosynthesis of heparan sulfate (HS) and the development of the chemical and chemo-enzymatic methods for the synthesis of heparan sulfate oligosaccharides. The HS synthesis methods provided in this chapter are promising for acquiring large collection of HS molecules.

Chapter 3 describes the chemical synthesis and functional characterization of novel chimeric NOD2/TLR2 agonists. In this study, our purpose was to evaluate the potential synergistic effects of NOD2 and TLR2 stimulation by a covalently linked ligand on the induction of proinflammatory cytokine secretion in vitro.

Chapter 4 and chapter 5 describe the chemical synthesis of 6-O modification tetrasaccharide precursors and further enzymatic diversification on these precursors to access to heparan sulfate oligosaccharides.

Start date and time
End date and time
Location
Academiegebouw, Domplein 29 & online (livestream link)
PhD candidate
L. Zhang
Dissertation
Synthesis and Functional Characterization of Novel Chimeric Vaccine Adjuvants & Chemo-Enzymatic Approaches to Synthesize Heparan Sulfate Oligosaccharides
PhD supervisor(s)
prof. dr. G.J.P.H. Boons
Co-supervisor(s)
dr. ir. D.T.S. Rijkers