11 October 2017 from 10:30 to 11:30

PhD Defence: Role of CNPY5 in B cell activation and immunoglobulin production

Activation of B cells into antibody-secreting plasma cells involves large changes in cellular morphology. The endoplasmic reticulum enlarges to accomodate the synthesis and assembly of immunoglobulins, in this process ER-resident chaperones and folding enzymes increase in abundance. One of the proteins that increases most in expression is CNPY5, the immune cell specific member of the CNPY family. This protein has been implicated in immunoglobulin folding and assembly, integrin secretion and B cell development. In this thesis we have characterised CNPY5 and the other members of the CNPY family, and showed that they are ER-resident proteins. We further characterise the role of CNPY5 in the production of IgM. In the absence of CNPY5 secretion of IgM Was reduced and assmebly inefficient. There was also an effect on the ER stress levels both before and during plasma cell differentiation. The results suggest that CNPY5 plays a role in the assembly of IgM and is required for high levels of immunoglobulin production.

Start date and time
11 October 2017 10:30
End date and time
11 October 2017 11:30
PhD candidate
N.G. (Naomi) Lodder
Dissertation
Role of CNPY5 in B cell activation and immunoglobulin production
PhD supervisor(s)
prof.dr. I. Braakman
Co-supervisor(s)
dr. P. van der Sluijs