PhD Defence: Involvement of CRISP2 in the organization of dense protein structures in porcine sperm

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Dissertation summary

In this thesis, we study cysteine rich secretory protein 2 (CRISP2) in pig sperm. This protein is present in dense protein structures in the sperm head and tail, structures that are essential for sperm functioning. They allow compaction and thus minimize the size of the sperm cell and are also responsible for the shape and robustness of the sperm cell. We showed that CRISP2 is involved in the organization of these dense protein structures. We also showed that CRISP2 is rapidly dissolved from the dense protein structures of sperm, immediately after fertilization. The redox state of the sperm cell and of the fertilized oocyte are likely the determinants for the CRISP2 behavior in building and dissociation of the dense protein structures. We showed that CRISP2 entraps a set of proteins during the formation of dense protein structures and these proteins are released after fertilization. Thus, the central role of CRISP2 to form dense protein structures in the testis and in the epididymal environments and to dissolve post fertilization from these structures deserve further attention with special emphasis on the role of CRISP2 in (de)condensation dynamics.

Start date and time
End date and time
Location
The Academiegebouw (Domplein 29) and digital
PhD candidate
M. Zhang
Dissertation
Involvement of CRISP2 in the organization of dense protein structures in porcine sperm
PhD supervisor(s)
dr. B.M. Gadella
prof. dr. J.B. Helms
More information
Full text via Utrecht University Repository