PhD defence: Enhancing Recombinant Monoclonal Antibody Production in CHO Cell Cultures: Leveraging the Power of Multivariate Analysis and Chemical Additives in Upstream Bioprocessing

This thesis explores two strategies to improve monoclonal antibody (mAb) production in CHO cells: multivariate data analysis (MVDA) with spectroscopic data and the use of chemical additives.

MVDA with UV-Vis spectroscopy offers a rapid and cost-effective method for monitoring crucial parameters such as cell density and antibody titer, particularly useful for resource-limited laboratories. Furthermore, this research investigates the impact of chemical additives like adenosine, cordycepin, and ectoine on cell proliferation and mAb production, aiming to increase yield and quality. In short, this research presents promising strategies for improving mAb production, especially in resource-constrained environments.