20 March 2019 from 10:30 to 11:30

PhD defence of Sajjad Mohammadian

Integrated Super Resolution Fluorescence Microscopy and Transmission Electron Microscopy

Correlative Light and Electron Microscopy (CLEM) is a robust method for studying microscopic objects and structures. On the one hand, light microscopy (usually Fluorescence Microscopy (FM)) provides high sensitivity, great specificity and a large field of view. On the other hand, electron microscopy (EM) has a magnificent ability to study nanometer scale structures in great detail. Combining these two techniques benefits from their individual strengths and has been done for several decades. However, to bring correlative microscopy to the world of high speed, high accuracy and user friendliness, several challenges have to be overcome. First, the resolution of FM is far worse than EM. This resolution gap hinders the proper comparison between target structures in FM and EM. Second, accurately overlaying fluorescence and electron microscopy images, essential to correlative microscopy, can be difficult. Finally, the speed of correlative microscopy experiments is usually low due to use of separate setups.
The first challenge has been tackled in recent years by the arrival of super resolution fluorescence microscopy (SR-FM) techniques. In this thesis, I focus on several challenges in correlative microscopy. I first introduce a novel method for accurately overlaying correlative microscopy images using fiducial markers and image processing algorithms. Next, I describe the successful integration of a super resolution fluorescence microscope inside a transmission electron microscope (TEM) and two proof of principle applications in biology and material science. Investigation of sample support properties and a promising dye for future applications using this integrated system form the last part of my study.

Start date and time
20 March 2019 10:30
End date and time
20 March 2019 11:30
PhD candidate
S. Mohammadian
Dissertation
Integrated Super Resolution Fluorescence Microscopy and Transmission Electron Microscopy
PhD supervisor(s)
prof. dr. H.C. Gerritsen