The course is a two-day laboratory workshop in Leiden. This training instructs the attendee in best practices procedures for in silico and in vitro assays related to acute and chronic cardiotoxicity.
Participants will learn among other things:
- Regulatory context to apply these methodologies as well as strength, weaknesses and future prospects (UCB - Dr Delaunois)
- Cell handling of human iPSC-derived cardiomyocytes (e.g. thawing procedure, cells seeding) (NCardia)
- Preparation of cryopreserved hiPSC-derived cardiomyocytes in 48/96 or 348 well plates (NCardia)
- Exposure of cells with cardiotoxic compounds using different readouts such as CiPA-like MEA (Micro-electrode array) and calcium flux assay (NCardia)
- Risk Assessment, validation, analysis interpretation and decision making using HiPS-cells for early drug discovery and development (Jannsen Pharma - Dr. Kreir)
- In silico assessment of the effects of various compounds in MEA/hiPSC-CM assays: modelling and numerical simulations (inria)
The training will start with half day lectures and will be followed by 1.5 days of practical training (Hands on-training) where participants, divided in small group will get the chance to manipulate and see the critical steps of the methods with the experts. This is a perfect way to quickly approach a method, as well as data analysis and interpretation.
Draft Agenda will be soon available.
The training will only take place if a minimum of 4 participants is reached.
Registration fee includes the participant booklet, catering and social dinner.