prof. dr. P. (Piet) Gros
P.Gros@uu.nl
Gegenereerd op 2015-07-31 19:26:10


Chair
Biomacromolecular Crystallography
Endowed by Utrecht University
Date of appointment 01.02.2002
Inaugural lecture date 14.04.2003
Profile

 

 

Research focus

Piet Gros studies the molecular mechanisms of recognition and regulation that underlie biological processes. His major expertise is in the area of protein crystallography methodology and its application to large human plasma proteins and cell-surface receptors and membrane proteins.

Regulation in cells and organisms is achieved by the interplay of proteins acting in concert to achieve a control over time and space. In many cases, the proteins involved are multi-domain proteins that function in large complexes. This enables a collective set of proteins to form a protein machinery that carries out elementary regulatory functions such as initiation, amplification, localization and inhibition. His research  goal is to reveal the mechanisms of tertiary and quaternary-induced activation in multi-domain proteins and multi-protein complexes that underlie the recognition and regulation processes.

Key research results include structural studies of (i) complement proteins and complexes, which have revealed the molecular mechanisms that underlie key steps in the human immune defense by the complement system, (ii) adhesion of von Willebrand factor (A1 domain) to its platelet receptor GpIb  and (iii) several bacterial outer-membrane proteins involved in pathogenicity. Piet Gros collaborates with national and international biomedical and biological research groups in these areas. The projects provide a structural basis for host-pathogen interactions and for understanding molecular disorders in diseases, which is crucial for structure-based approaches to develop novel drugs and vaccines.

Piet Gros is extremely grateful for the many excellent contributions of the PhD students, post-doctoral fellows and technicians in his lab.

For more information see: Research page of the Piet Gros' lab

 

Honours

  • EMBO member (since 2013)
  • Knight in the Order of the Netherlands Lion (Ridder in de Orde van de Nederlandse Leeuw; since 2013).
  • Spinoza prize of the Netherlands Organisation for Scientific Research, NWO (2010).
  • Member of the Royal Academy of Arts and Sciences, KNAW (since 2010).
  • ERC Advanced Grant, EU (2008).
  • E.J. Cohn lecturer, IDI Harvard Medical School USA (2008).
  • Pionier Award, Council for Chemical Sciences, NWO-CW (2000).
  • Young Chemist Awards, Council for Chemical Sciences, NWO-CW (1997 and 1999).

 

Key publications

  • Complement is activated by IgG hexamers assembled at the cell surface. C.A. Diebolder*, F.J. Beurskens*, R.N. de Jong, R.I. Koning, K. Strumane, M.A. Lindorfer, M. Voorhorst, D. Ugurlar, S. Rosati, A.J.R. Heck, J.G.J. van de Winkel, I.A. Wilson, A.J. Koster, R.P. Taylor, E. Ollmann Saphire, D.R. Burton, J. Schuurman, P. Gros# and P.W.H.I. Parren#, Science 343,1261-1263 (2014). *These authors contributed equally. #Corresponding authors. 
  • Structure of stem cell growth factor R-spondin 1 in complex with the ectodomain of its receptor LGR5. W.C. Peng*, W. de Lau*, F. Forneris, J.C.M. Granneman, M. Huch, H. Clevers and P. Gros, Cell Reports 3, 1885-1892 (2013). *These authors contributed equally.
  • Modelling dynamics in protein crystal structures by ensemble refinement. B.T. Burnley, P.V. Afonine, P.D. Adams and P. Gros, eLife 1:e00311. doi: 10.7554/elife.00311. (2012).
  • Assembly and regulation of the membrane attack complex based on structures of C5b6 and sC5b9. M.A. Hadders*, D. Bubeck*, P. Roversi, S. Hakobyan, F. Forneris, B.P. Morgan, M.K. Pangburn, O. Llorca, S.M. Lea and P. Gros, Cell Reports 1, 200-207 (2012). *These authors contributed equally.
  • Structures of C3b in complex with factors B and D give insight into complement convertase formation. F. Forneris, D. Ricklin, J. Wu, A. Tzekou, R.S. Wallace, J. Lambris and P. Gros, Science 330, 1816-1920 (2010).
  • Structural and functional implications of the alternative complement pathway C3 convertase stabilized by a staphylococcal inhibitor. S.H.M. Rooijakkers*, J. Wu*, M. Ruyken, R. van Domselaar, K.L. Planken, A. Tzekou, D. Ricklin, J.D. Lambris, B.J.C. Janssen, J.A.G. van Strijp and P. Gros, Nature Immunology 10, 721-727 (2009). *These authors contributed equally.
  • Structure of complement fragment C3b-factor H and implications for host protection by complement regulators. J. Wu, Y.-Q. Wu, D. Ricklin, B.J.C. Janssen, J.D. Lambris and P. Gros, Nature Immunology 10, 728-733 (2009).
  • Complement driven by conformational changes. P. Gros, F.J. Milder and B.J.C. Janssen, Nature Reviews Immunology 8, 48-58 (2008).
  • Structure of C8α-MACPF reveals mechanism of membrane attack in complement immune defense. M.A. Hadders, D.X. Beringer and P. Gros, Science 317, 1552-1554 (2007).
  • Factor B structure provides insights into the activation of the central protease of the complement system. F.J. Milder, L. Gomes, A. Schouten, B.J.C. Janssen, E.G. Huizinga, R.A. Romijn, W. Hemrika, A. Roos, M.R. Daha and P. Gros, Nature Structural and Molecular Biology 14, 224-228 (2007).
  • Structure of C3b reveals conformational changes underlying complement activity. B.J.C. Janssen, A. Christodoulidou, A. McCarthy, J.D. Lambris and P. Gros, Nature 444, 213-216 (2006).
  • Structures of complement component C3 provide insights in function and evolution of immunity. B.J.C. Janssen, E.G. Huizinga, H.C.A. Raaijmakers, A. Roos, M.R. Daha, K. Nilsson-Ekdahl, B. Nilsson and P. Gros, Nature 437, 505-511 (2005).
  • Structure of the translocator domain of a bacterial autotransporter. C.J. Oomen, P. van Ulsen, P. van Gelder, M. Feijen, J. Tommassen and P. Gros, EMBO Journal 23, 1257-1266 (2004).
  • Structures of Glycoprotein Ibα and its complex with von Willebrand Factor A1 domain. E.G. Huizinga, S. Tsuji, R.A.P. Romijn, M.E. Schiphorst, Ph.G. de Groot, J.J. Sixma and P. Gros, Science 297, 1176-1179 (2002).
  • Crystal structure of the Outer Membrane Protease OmpT from Escherichia coli suggests a novel catalytic site. L. Vandeputte-Rutten, R.A. Kramer, J. Kroon, N. Dekker, M.R. Egmond and P. Gros, EMBO Journal 20, 5033-5039 (2001).
  • Specific chemical and structural damage to proteins produced by synchrotron radiation. M. Weik. R.B.G. Ravelli, G. Kryger, S. McSweeney, M.L. Raves, M. Harel, P. Gros, I. Silman, J. Kroon and J.L. Sussman, PNAS U.S.A. 97, 623-628 (2000).
  • Adhesion of human β2-Glycoprotein I to phospholipids based on its crystal structure. B. Bouma, Ph.G. de Groot, J.M.H. van den Elsen, R.B.G. Ravelli, A. Schouten, M.J.A. Simmelink, R.H.W.M. Derksen, J. Kroon and P. Gros, EMBO Journal 18, 5166-5174 (1999).      
  • Crystallography and NMR System: a new software system for macromolecular structure determination. A.T. Brunger, P.D. Adams, G.M. Clore, W.L. DeLano, P. Gros, R.W. Grosse-Kunstleve, J.-S. Jiang, J. Kuszweski, M. Nilges, N.S. Pannu, R.J. Read, L.M. Rice, T. Simonson and G.L. Warren, Acta Crystallographica D54, 905-921 (1998).
  • Inclusion of Thermal Motion in Crystallographic Structures by Restrained Molecular Dynamics. P. Gros, W.F. van Gunsteren and W.G.J. Hol, Science 249, 1149-1152 (1990).
 
Involved in the following study programme(s)
Scientific expertise

Please, see link for research webpages
Gegenereerd op 2015-07-31 19:26:10
Curriculum vitae

Piet Gros (Dokkum, July 31, 1962)

Doctorandus (MSc) cum laude (highest degree) Chemistry, University of Groningen, 1985

Doctor (PhD) cum laude (highest degree) Protein Crystallography, promotor prof. W.G.J. Hol, University Groningen, 1990.

Post-doctoral fellow, lab of prof. W.F. van Gunsteren, ETH Zurich, Switzerland, 1990-1992.

Post-doctoral fellow, lab of prof. A.T. Brunger, Yale University New Haven CT USA, 1992-1994.

Senior researcher, Chemistry, Utrecht University, 1994-1998.

Assistant Professor, Chemistry, Utrecht University, 1998-2002.

Professor, Chemistry, Utrecht University, 2002-present

Chair dept. Chemistry, Utrecht University, 2007-2010.

Director Bijvoet Center, Utrecht University, 2012-present.

Gegenereerd op 2015-07-31 19:26:10
All publications
  2014 - Scholarly publications
Diebolder, Christoph A, Beurskens, Frank J, de Jong, Rob N, Koning, Roman I, Strumane, Kristin, Lindorfer, Margaret A, Voorhorst, Marleen, Ugurlar, Deniz, Rosati, Sara, Heck, Albert J R, van de Winkel, Jan G J, Wilson, Ian A, Koster, Abraham J, Taylor, Ronald P, Saphire, Erica Ollmann, Burton, Dennis R, Schuurman, Janine, Gros, Piet & Parren, Paul W H I (2014). Complement is activated by IgG hexamers assembled at the cell surface. Science, 343 (6176), (pp. 1260-3) (4 p.).
Forneris, Federico, Burnley, B. Tom & Gros, Piet (01.03.2014). Ensemble refinement shows conformational flexibility in crystal structures of human complement factor D. Acta crystallographica. Section D, biological crystallography, 70 (3), (pp. 733-743) (11 p.).
Vissers, Lisenka ELM, Bonetti, Monica, Paardekooper Overman, Jeroen, Nillesen, Willy M., Frints, Suzanna G M, de Ligt, Joep, Zampino, Giuseppe, Justino, Ana, Machado, José C., Schepens, Marga, Brunner, Han G., Veltman, Joris A., Scheffer, Hans, Gros, Piet, Costa, José L., Tartaglia, Marco, van der Burgt, Ineke, Yntema, Helger G. & den Hertog, Jeroen (18.06.2014). Heterozygous germline mutations in A2ML1 are associated with a disorder clinically related to Noonan syndrome. European Journal of Human Genetics
de Lau, Wim, Peng, Weng Chuan, Gros, Piet & Clevers, Hans (15.02.2014). The R-spondin/Lgr5/Rnf43 module - Regulator of Wnt signal strength. Genes and Development, 28 (4), (pp. 305-316) (12 p.).
  2013 - Scholarly publications
Burnley, B.T. & Gros, P. (2013). phenix.ensemble_refinement: a test study of apo and holo BACE1. Computational crystallography newsletter, 4, (pp. 51-58) (7 p.).
Peng, W.C., de Lau, W., Forneris, F., Granneman, J.C.M., Clevers, H.C. & Gros, P. (2013). Structure of Stem Cell Growth Factor R-spondin 1 in Complex with the Ectodomain of Its Receptor LGR5. Cell Reports [E], 3 (6), (pp. 1885-1892) (8 p.).
Peng, W.C., de Lau, W., Madoori, P.K., Forneris, F., Granneman, J.C.M., Clevers, H. & Gros, P. (2013). Structures of Wnt-Antagonist ZNRF3 and Its Complex with R-Spondin 1 and Implications for Signaling. PLoS ONE, 8 (12), (pp. e83110) (10 p.).
  2012 - Scholarly publications
Hadders, M.A., Bubeck, D., Roversi, P., Hakobyan, S., Forneris, F., Morgan, B.P., Pangburn, M.K., Llorca, O., Lea, S.M. & Gros, P. (2012). Assembly and regulation of the membrane attack complex based on structures of C5b6 and sC5b9. Cell Reports [E], 1 (3), (pp. 200-207) (8 p.).
van Galen, Josse, Olrichs, Nick K, Schouten, Arie, Serrano, Ramon L, Nolte-'t Hoen, Esther N M, Eerland, Ruud, Kaloyanova, Dora, Gros, Piet & Helms, J Bernd (2012). Interaction of GAPR-1 with lipid bilayers is regulated by alternative homodimerization. Biochimica et Biophysica Acta, 1818 (9), (pp. 2175-2183) (9 p.). Copyright © 2012 Elsevier B.V. All rights reserved..
Burnley, B.T., Afonine, P.V., Adams, P.D. & Gros, P. (2012). Modelling dynamics in protein crystal structures by ensemble refinement. eLife, 1, (pp. 1-29) (29 p.).
Volokhina, E.B., Westra, D., Xue, X, Gros, P., van der Kar, N.C.A.J. & van den Heuvel, L. (2012). Novel C3 mutation p.Lys65Gln in aHUS affects complement factor H binding. Pediatric Nephrology, 27, (pp. 1519-1524) (6 p.).
Forneris, F., Wu, J. & Gros, P. (2012). The modular serine proteases of the complement cascade. Current opinion in structural biology, 22 (3), (pp. 333-341) (9 p.).
  2011 - Scholarly publications
Roussel-Jazédé, V.M.C., van Gelder, P., Sijbrandi, R., Rutten, L., Otto, B.R., Luirink, J., Gros, P., Tommassen, J.P.M. & van Ulsen, J.P. (2011). Channel properties of the translocator domain of the autotransporter Hbp of Escherichia coli. Molecular Membrane Biology, 28 (3), (pp. 158-170) (13 p.).
Gros, P. (2011). In self-defense. Nature structural and molecular biology, 18 (4), (pp. 401-402) (2 p.).
  2010 - Scholarly publications
Serruto, D., Rappuoli, R., Scarselli, M., Gros, P. & van Strijp, J.A.G. (2010). Molecular mechanisms of complement evasion: learning from staphylococci and meningococci. Nature Reviews. Microbiology, 8, (pp. 393-399) (7 p.).
Forneris, F., Ricklin, D., Wu, J., Tzekou, A., Wallace, R. S., Lambris, J.D. & Gros, P. (2010). Structures of C3b in complex with factors B and D give insight into complement convertase formation. Science, 330 (6012), (pp. 1816-1820) (5 p.).
  2009 - Scholarly publications
Rutten, L., Mannie, J.-P.B.A., Stead, C.M., Raetz, C.R.H., Reynolds, C.M., Bonvin, A.M.J.J., Tommassen, J.P.M., Egmond, M.R., Trent, M.S. & Gros, P. (2009). Active-site architecture and catalytic mechanism of the lipid A deacylase LpxR of Salmonella typhimurium. Proceedings of the National Academy of Sciences of the United States of America, 106 (6), (pp. 1960-1964) (5 p.).
Poodt, P.W.G., Heijna, M.C.R., Schouten, A., Gros, P., van Enckevort, W.J.P. & Vlieg, E. (2009). Simple geometry for diffusion limited protein crystal growth: harnessing gravity to suppress convection. Crystal growth & design, 9 (2), (pp. 885-888) (4 p.).
Rutten, L., Wieczorek, B., Mannie, J.-P.B.A., Kruithof, C.A., Dijkstra, H.P., Egmond, M.R., Lutz, M., Klein Gebbink, R.J.M., Gros, P. & van Koten, G. (2009). Solid-state structural characterization of cutinase-ECE-pincer-metal hybrids. Chemistry-A European Journal, 15 (17), (pp. 4270-4280) (11 p.).
Rooijakkers, S.H.M, Wu, J., Ruyken, M., van Domselaar, R., Planken, K.L., Tzekou, A., Ricklin, D., Lambris, J.D., Janssen, B.J., van Strijp, J.A.G. & Gros, P. (2009). Structural and functional implications of the alternative complement pathway C3 convertase stabilized by a staphylococcal inhibitor. Nature immunology, 10 (7), (pp. 721-727) (7 p.).
  2008 - Scholarly publications
Gros, P., Milder, F.J. & Janssen, B.J.C. (2008). Complement driven by conformational. Nature Reviews. Immunology, 8, (pp. 48-58) (11 p.).
  2007 - Scholarly publications
Herczenik, E., Bouma, B., Korporaal, J.A., Strangi, R., Zeng, Q., Gros, P., van Eck, M., van Berkel, T.J.C., Gebbink, M.F.B.G. & Akkerman, J.W.N. (2007). Activation of human platelets by misfolded proteins. Arteriosclerosis, Thrombosis and Vascular Biology, 27, (pp. 1657-1665) (10 p.).
Morlot, C., Hemrika, W., Romijn, R.A., Gros, P., Cusack, S. & McCarthy, A.A. (2007). Cloning, expression, crystallization and preliminary X-ray analysis of the first two Ig domains from human Roundabout 1 (Robo1). Acta crystallographica. Section F, Structural biology and crystallization communications, F63, (pp. 689-691) (3 p.).
Janssen, B.J.C., Read, R.J., Brunger, A.T. & Gros, P. (2007). Crystallographic evidence for deviating C3b structure?. Nature, 448, (pp. E1-E2) (2 p.).
Arlaud, G.J., Barlow, P.N., Gaboriaud, C., Gros, P. & Narayana, S.V.L. (2007). Deciphering complement mechanisms: The contributions of structural biology. Molecular Immunology, 44, (pp. 3809-3822) (14 p.).
Milder, F.J., Gomes, L., Schouten, A., Janssen, B.J.C., Huizinga, E.G., Romijn, R.A., Hemrika, W., Roos, A, Daha, M.R. & Gros, P. (2007). Factor B structure provides insights into activation of the central protease of the complement system. Nature structural and molecular biology, 14, (pp. 224-228) (5 p.).
Morlot, C., Hemrika, W., Romijn, R.A., Gros, P., Cusack, S. & McCarthy, A.A. (2007). Production of Slit2 LRR domains in mammalian cells for structural studies and the structure of human Slit2 domain 3. Acta crystallographica. Section D, biological crystallography, D63, (pp. 961-978) (8 p.).
Rooijakkers, S.H.M, Milder, F.J., Bardoel, B.W., Ruyken, M., van Strijp, J.A.G. & Gros, P. (2007). Staphylococcal complement inhibitor: structure and active sites. Journal of Immunology, 179 (5), (pp. 2989-2998) (10 p.).
Morlot, C., Thielens, N.M., Ravelli, R.B.G., Hemrika, W., Romijn, R.A., Gros, P., Cusack, S. & McCarthy, A.A. (2007). Structural insights into the Slit-Robo complex. Proceedings of the National Academy of Sciences of the United States of America, 5, (pp. 1538-1544) (6 p.).
Hadders, M.A., Beringer, D. & Gros, P. (2007). Structure of C8a-MACPF Reveals Mechanism of Membrane Attack in Complement Immune Defense. Science, 317, (pp. 1552-1554) (3 p.).
Janssen, B.J.C., Halff, E.F., Lambris, J.D. & Gros, P. (2007). Structure of Compstatin in Complex with Complement Component C3c Reveals a New Mechanism of Complement Inhibition. Journal of Biological Chemistry, 282, (pp. 29241-29247) (7 p.).
  2006 - Scholarly publications
Janssen, B.J.C. & Gros, P. (2006). Conformational complexity of complement component C3. In J.D. Lambris (Eds.), Current topics in complement (pp. 291-312) (406 p.). New York: Springer.
Rutten, L., Geurtsen, J.J.G., Lambert, W., Smolenaers, J.J.M., Bonvin, A.M., de Haan, A., van der Ley, P., Egmond, M.R., Gros, P. & Tommassen, J.P.M. (2006). Crystal structure and catalytic mechanism of the LPS 3-O-deacylase PagL from Pseudomonas aeruginosa. Proceedings of the National Academy of Sciences of the United States of America, 103, (pp. 7071-7076) (6 p.).
Brown, K.M., Kondeatis, E., Vaughan, R.W., Kon, S.P., Farmer, C.K.T., Taylor, J.D., He, X., Johnston, A., Horsfield, C., Janssen, B.J.C., Gros, P., Zhou, W., Sacks, S.H. & Sheerin, N.S. (2006). Influence of Donor C3 Allotype on Late Renal-Transplantation Outcome. New England Journal of Medicine, 354, (pp. 2014-2023) (10 p.).
Wirtz, K.W.A., Schouten, A. & Gros, P. (2006). Phosphatidylinositol transfer proteins: From closed for transport to open for exchange. Advances in enzyme regulation, 46, (pp. 301-311) (11 p.).
Janssen, B.J.C. & Gros, P. (2006). Structural insights into the central complement component C3. Molecular Immunology, 44, (pp. 3-10) (8 p.).
van Ulsen, J.P., Gros, P. & Tommassen, J.P.M. (2006). Structure and function of the translocator domain of bacterial autotransporters. In R. Grisshammer & S. Buchanan (Eds.), Structural biology of membrane proteins (pp. 270-287) (18 p.). Cambridge, U.K..: RSC.
Janssen, B.J.C., Christodoulidou, A., McCarthy, A.A., Lambris, J.D. & Gros, P. (2006). Structure of C3b reveals conformational changes that underlie complement activity. Nature, 444, (pp. 213-216) (4 p.).
Milder, F.J., Raaijmakers, H.C.A., Vandeputte, D.A.A., Schouten, A., Huizinga, E.G., Romijn, R.A., Hemrika, W., Roos, A, Daha, M.R. & Gros, P. (2006). Structure of complement component C2a: implications for convertase formation and substrate binding. Structure, 14 (10), (pp. 1587-1597) (11 p.).
  2005 - Scholarly publications
Oomen, C.J., Hoogerhout, P., Kuipers, B., Vidarsson, G., van Alphen, Loek & Gros, P. (2005). Crystal Structure of an Anti-meningococcal Subtype P1.4 PorA Antibody Provides Basis for Peptide-Vaccine Design. Journal of Molecular Biology, 351, (pp. 1070-1080) (11 p.).
Janssen, B.J.C., Huizinga, E.G., Raaijmakers, H.C.A., Roos, A, Daha, M.R., Nilsson-Ekdahl, K., Nilsson, B. & Gros, P. (2005). Structures of complement component C3 provide insights into the function and evolution of immunity. Nature, 437, (pp. 505-511) (7 p.).
Weik, M., Schreurs, A.M.M., Leiros, H.-K. S., Zaccai, G., Ravelli, R.B.G. & Gros, P. (2005). Supercooled liquid-like solvent in trypsin crystals: implications for crystal annealing and temperature-controlled Z-ray radiation damage studies. Journal of Synchrotron Radiation, 12, (pp. 310-317) (8 p.).
  2004 - Scholarly publications
Oomen, C.J., van Ulsen, J.P., van Gelder, P., Feijen, M., Tommassen, J.P.M. & Gros, P. (2004). Structure of the translocator domain of a bacterial autotransporter. EMBO Journal, 23, (pp. 1257-1266) (10 p.).
Scheres, S.H.W. & Gros, P. (2004). The potentials of conditional optimization in phasing and model building of protein crystal structures. Acta crystallographica. Section D, biological crystallography, 60, (pp. 2202-2209) (8 p.).
  2003 - Scholarly publications
Vandeputte-Rutten, L., Bos, M.P., Tommassen, J.P.M. & Gros, P. (2003). Crystal structure of neisserial surface protein A (NspA), a conserved outer membrane protein with vaccine potential. Journal of Biological Chemistry, 278 (27), (pp. 24825-24830) (6 p.).
Dekker, C.G., de Kruijff, B. & Gros, P. (2003). Crystal structure of SecB from Escherichia coli. Journal of Structural Biology, 144, (pp. 313-319) (7 p.).
Scheres, S.H.W. & Gros, P. (2003). Development of a force field for conditional optimization of protein structure. Acta crystallographica. Section D, biological crystallography, 59, (pp. 438-446) (9 p.).
Oomen, C.J., Hoogerhout, P., Bonvin, A.M.J.J., Kuipers, B., Brughhe, H., Timmermans, H., Hasely, S.R., van Alphen, L. & Gros, P. (2003). Immunogenicity of peptide-vaccine candidates predicted by molecular dynamics simulations. Journal of Molecular Biology, 328, (pp. 1083-1089) (7 p.).
Agianian, B., Tucker, P.A., Schouten, A., Leonard, K., Bullard, B. & Gros, P. (2003). Structure of a Drosophila Sigma Class Glutathione S-transferase Reveals a Novel Active Site Topography Suited for Lipid Peroxidation Products. Journal of Molecular Biology, 326, (pp. 151-165) (15 p.).
  2002 - Scholarly publications
Weik, M., Berges, J., Raves, M., Gros, P., McSweeney, S., Silman, I., Sussman, J.L., Houee-Levin, C. & Ravelli, R.B.G. (2002). Evidence for the formation of disulfide radicals in protein crystals upon X-ray irridiation. Journal of Synchrotron Radiation, 9, (pp. 342-346) (5 p.).
Kramer, R.A., Brandenburg, K., Vandeputte-Rutten, L., Werkhoven, M., Gros, P., Dekker, N. & Egmond, M.R. (2002). Lipopolysaccharide regions involved in the activation of Escherichia coli outer membrane protease OmpT. European Journal of Biochemistry, 269, (pp. 1746-1752) (7 p.).
Vandeputte-Rutten, L. & Gros, P. (2002). Novel proteases: common themes and surprising features. Current opinion in structural biology, 12, (pp. 704-708) (5 p.).
Schouten, A., Agianian, B., Westerman, J., Kroon, J., Wirtz, K.W.A. & Gros, P. (2002). Structure of apo-phosphatidylinositol transfer protein a provides insight into membrane association. EMBO Journal, 21 (9), (pp. 2117-2121) (5 p.).
Huizinga, E.G., Tsuji, S., Romijn, R.A.P., Schiphorst, M.E., de Groot, P.G., Sixma, J.J. & Gros, P. (2002). Structures of glycoprotein Iba and its complex with von Willebrand Factor A1 domain. Science, 297, (pp. 1176-1179) (4 p.).
van Tiel, C.M., Schouten, A., Snoek, G.T., Gros, P. & Wirtz, K.W.A. (2002). The structure of phosphatidylinositol transfer protein a reveals sites for phospholipid binding and membrane association with major implications for its function. FEBS letters, 531, (pp. 69-73) (5 p.).
  2001 - Scholarly publications
Bouma, B.N., Westerman, J., Dekker, N., Gros, P. & Wirtz, K.W.A. (2001). Activation of phosphatidylinositol transfer protein alfa and beta isoforms from inclusion bodies. Biochimica et Biophysica Acta, 1546, (pp. 216-225) (10 p.).
Dekker, C., Agianian, B., Weik, M., Zaccai, G., Kroon, J., de Kruijff, B. & Gros, P. (2001). Biophysical characterization of the influence of salt on tetrameric SecB. Biophysical Journal, 81, (pp. 455-462) (8 p.).
Scheres, S.H.W. & Gros, P. (2001). Conditional optimization: a new formalism for protein structure refinement. Acta Crystallographica, D57, (pp. 1820-1828) (9 p.).
Vandeputte-Rutten, L., Kramer, R.A., Kroon, J., Dekker, N., Egmond, M.R. & Gros, P. (2001). Crystal structure of the outer membrane protease OmpT from Escherichia coli suggests a novel catalytic site EMBO Journal. EMBO Journal, 20, (pp. 5033-5039) (7 p.).
Kramer, R.A., Vandeputte-Rutten, L., de Roon, G.J., Gros, P., Dekker, N. & Egmond, M.R. (2001). Identification of essential acidic residues of outer membrane protease OmpT supports a novel active site. FEBS letters, 505, (pp. 426-430) (5 p.).
Romijn, R.A.P., Bouma, B., Wuyster, W., Gros, P., Kroon, J., Sixma, J.J. & Huizinga, E.G. (2001). Identification of the collagen-binding site of the von Willebrand Factor A3-domain. Journal of Biological Chemistry, 276, (pp. 9985-9991) (7 p.).
Kroon, J., Weik, M., Kryger, G., Schreurs, A.M.M., Bouma, B.N., Silman, I., Sussman, J.L. & Gros, P. (2001). Solvent behaviour in flash-cooled protein crystals at cryogenic temperatures. Acta Crystallographica, D57, (pp. 566-573) (8 p.).
Kroon, J., Weik, M., Ravelli, R.B.G., Silman, I., Sussman, J.L. & Gros, P. (2001). Specific protein dynamics near the solvent glass transition assayed by radiation-induced structural changes. Protein Science, 10, (pp. 1953-1961) (9 p.).
Brunger, A.T., Adams, P.D., DeLano, W.L., Gros, P., Grosse-Kunstleve, R.W., Jiang, J.-S., Pannu, N.S., Read, R.J., Rice, L.M. & Simonson, T. (2001). The structure determination language of the crystallography anf NMR system. In M.G. Rossmann & E. Arnolds (Eds.), International Tables for X-ray Crystallography (Volume F) Crystallography of Biological Macromolecules Dordrecht: Kluwer Academic Publishers.
Huizinga, E.G., Schouten, A., Connolly, T.M., Kroon, J., Sixma, J.J. & Gros, P. (2001). The structure of leech anti-plated protein, an inhibitor of haemostasis. Acta crystallographica. Section D, biological crystallography, D 57, (pp. 1071-1078) (8 p.).
  2001 - Professional publications
Agianian, B., Clayton, P., Leonard, J.M., Tucker, P.A. & Gros, P. (2001). Crystallization and preliminary X-ray analysis of Drosophila glutathione S-transferase-2. Acta crystallographica. Section D, biological crystallography, D57, (pp. 725-727) (3 p.).
  2000 - Scholarly publications
Oomen, C.J., Bonvin, A.M.J.J., Haseley, S.R., Hoogerhout, P., van Alphen, L., Kroon, J. & Gros, P. (2000). Rational design of a subtype-specific peptide vaccine against Neisseria meningitidis. In J.P. Tam G. Barany G.B. Fields (Eds.), Peptides for a New Millenium (pp. 702-703) (2 p.). Dordrecht, The Netherlands: Kluwer Academic Publishers, 16th American Peptide Symposium.
Weik, M., Ravelli, R.B.G., Kryger, G., McSweeney, S., Raves, M., Harel, M., Gros, P., Silman, I., Kroon, J. & Sussman, J.L. (2000). Specific chemical and structural damage to proteins produced by synchrotron radiation. Proceedings of the National Academy of Sciences of the United States of America, 97, (pp. 623-628) (6 p.).
de Groot, P.G., Bouma, B.N., Lutters, B.C.H., Simmelink, M.J.A., Derksen, R.H.W.M. & Gros, P. (2000). Structure-function studies on B2-glycoprotein I. Journal of Autoimmunity, 15, (pp. 87-89) (3 p.).
de Groot, P.G., Bouma, B., Lutters, B.C.H., Simmelink, M.J.A., Derksen, R.H.W.M. & Gros, P. (2000). Structure-function studies on beta2-Glycoprotein I. Journal of Autoimmunity, 15, (pp. 87-89) (3 p.).
  2000 - Popularising publications
Kroon, J. & Gros, P. (2000). "Sneak Preview" Stralingsschade. Chemsch2Weekblad 4, (pp. 5-5) (1 p.).
  1999 - Scholarly publications
Bouma, B.N., de Groot, P.G., van den Elsen, J.M.H., Ravelli, R.B.G., Simmelink, M.J.A., Derksen, R.H.W.M., Kroon, J. & Gros, P. (1999). Adhesion mechanism of human beta 2-glycoprotein I to phospholipids based on its crystal structure. EMBO Journal, 18, (pp. 5166-5174) (9 p.).
Bouma, B., de Groot, Ph.G., van den Elsen, J.M.H., Ravelli, R.B.G., Schouten, A., Simmelink, M.J.A., Derksen, R.H.W.M., Kroon, J. & Gros, P. (1999). Adhesion of human beta2-Glycoprotein I to phospholipids based on its crystal structure. EMBO Journal, 18, (pp. 5166-5174) (9 p.).
van den Elsen, J.M.H., Vandeputte-Rutten, L., Kroon, J. & Gros, P. (1999). Bactericidal antibody recognition of meningococcal PorA by induced fit: comparison of liganded and unliganded Fab structures. Journal of Biological Chemistry, 274, (pp. 1495-1501) (7 p.).
Dekker, C.G., de Korte-Kool, G., Kroon, J., de Kruijff, B. & Gros, P. (1999). Crystals of acetylated SecB diffract to 2.3 Angstrom resolution. Journal of Structural Biology, 127, (pp. 237-242) (6 p.).
  1998 - Scholarly publications
Brunger, A.T., Adams, P.D., Clore, G.M., DeLano, W.L., Gros, P., Grosse-Kunstleve, R.W., Jiang, J.-S., Kuszweski, J., Nilges, M., Pannu, N.S., Read, R.J., Rice, L.M., Simonson, T. & Warren, G.L. (1998). Crystallography and NMR System: a new software system for macromolecular structure determination. Acta crystallographica. Section D, biological crystallography, D54, (pp. 905-921) (17 p.).
Werten, S., Stelzer, G., Goppelt, A., Langen, F.M., Gros, P., Timmers, H.T.M., van der Vliet, P.C. & Meisterernst, M. (1998). Interaction of PC4 with melted DNA inhibits transcription. EMBO Journal, 17, (pp. 5103-5111) (9 p.).
  1997 - Scholarly publications
van den Elsen, J.M.H., Herron, J.N., Hoogerhout, P., Poolman, J.T., Boel, E., Logtenberg, T., Wilting, J., Crommelin, D.J.A., Kroon, J. & Gros, P. (1997). Bactericidal Antibody Recognition of a PorA Epitope of Neisseria meningitidis: Crystal structure of a Fab fragment in complex with a fluorescein-conjugated peptide. Proteins: Structure function and bioinformatics, 29 (1), (pp. 113-125) (13 p.).
Huizinga, E.G., van der Plas, R.M., Kroon, J., Sixma, J.J. & Gros, P. (1997). Crystal structure of the A3 domain of human von Willebrand factor: implications for collagen binding. Structure, 5, (pp. 1147-1156) (10 p.).
Brandsen, J., Werten, S., van der Vliet, P.C., Meisterernst, M., Kroon, J. & Gros, P. (1997). C-Terminal domain of transcription cofactor PC4 reveals a dimeric ssDNA binding site. Nature Structural Biology, 4, (pp. 900-903) (4 p.).
Brandsen, J., Werten, S., van der Vliet, P.C., Meisterernst, M., Kroon, J. & Gros, P. (1997). C-terminal domain of transcription cofactor PC4 reveals dimeric ssDNA binding site. Nature Structural Biology, 4, (pp. 900-904) (5 p.).
Sixma, J.J., van Zanten, G.H., Huizinga, E.G., van der Plas, R.M., Verkleij, M., Wu, Y.P., Gros, P. & de Groot, P.G. (1997). Platelet adhesion to collagen: an update. Thrombosis and Haemostasis, 78, (pp. 434-438) (5 p.).
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Gegenereerd op 2015-07-31 19:26:10

My research group uses protein crystallography to study bio-molecular recognition and regulation processes with emphasis on bio-medically important proteins, in particular in the area of infection and immunity. Regulation in cells and organisms is achieved by the interplay of proteins acting in concert to achieve a control over time and space. In many cases, the proteins involved are multi-domain proteins that function in large complexes. This enables a collective set of proteins to form a protein machinery that carries out elementary regulatory functions such as initiation, amplification, localization and inhibition. Our goal is to reveal the mechanisms of tertiary and quaternary-induced activation in multi-domain proteins and multi-protein complexes that underlie these recognition and regulation processes.

Over the last few years we have mainly focused on infection and immunity, in particular (1) the immune response by the complement system and complement-evasion mechanisms of pathogens and (2) outer-membrane proteins of pathogenic bacteria. We collaborate with national and international biomedical and biological research groups in these areas. The projects provide a structural basis for host-pathogen interactions and for understanding molecular disorders in diseases, which is crucial for structure-based approaches to develop novel drugs and vaccines.

In addition, we remain to have a keen interest in crystallographic methodology with respect to the phase problem and structure refinement.

 

 

The project information listed below is reproduced from an internal university database.

Project:
Institute for Chemical Immunology
01.01.2014 to 31.12.2023
General project description

The Institute for Chemical Immunlogy is an NWO Gravitation project coordinator by Leiden University. It will define and exploit a new field termed chemical immunology and train a novel generation of interdisciplinary scientists. It aims to promote academic excellence; with economic and clinical translation of new treatment options for a large population of patients that currently only have limited treatment options.

Role Researcher Funding Utrecht University Project members UU
External project members:
prof. dr. Sjaak Neefjes (NKI; coordinator)
prof. dr. Hermen Overkleeft (LU)
prof. dr. Carl Figdor (RU)
prof. dr. Ton Schumacher (NKI)
Project:
ManiFold
01.09.2012 to 31.08.2016
General project description

ManiFold is an Innovative Doctoral Programme organised by members of the Bijvoet Center for Biomolecular Research and its Graduate Research School, at Utrecht University in the Netherlands. The European Union selected the most innovative doctoral programmes in Europe for funding in the prestigious Marie-Curie Initial Training Networks scheme. ManiFold was the only Innovative Doctoral Programme in the Life Sciences funded in 2012.

Role Researcher Funding EU grant Project members UU

Gegenereerd op 2015-07-31 19:26:11
Additional functions and activities

Member of Scientific Advisory Board of The Aegean Conferences (Philadelphia, USA)

Member of Scientific Advisory Board of Utrecht-Protein Express B.V. (Utrecht)

Chair of Board of Dutch Complement Platform

Gegenereerd op 2015-07-31 19:26:11
Full name
prof. dr. P. Gros Contact details
Hugo R. Kruytgebouw

Padualaan 8
Room N806
3584 CH  UTRECHT
The Netherlands


Phone number (direct) +31 30 253 3127
Phone number (department) +31 30 253 3502
Email
P.Gros@uu.nl
Fax +31 30 253 3940
Postal address
Postbus 80.054
3508 TB    UTRECHT
The Netherlands
Gegenereerd op 2015-07-31 19:26:11
Last updated 16.12.2014